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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Lingkungan AL KAUNIYAH Jurnal Kimia dan Kemasan Journal of Tropical Biodiversity and Biotechnology Caraka Tani: Journal of Sustainable Agriculture BIOEDUSCIENCE Spektra: Jurnal Fisika dan Aplikasinya Pharmacoscript Sainstech Farma: Jurnal Ilmu Kefarmasian Jurnal Kimia dan Kemasan Biodiversitas Journal of Biological Diversity Nusantara Bioscience
Windri Handayani
Department Of Biology, Faculty Of Mathematics And Natural Sciences, Universitas Indonesia, Depok
Aerospace Engineering Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Education Electrical & Electronics Engineering Energy Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Physics Public Health Other

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The Development of Gametophyte Sterilization Method for Liverworts Acrolejeunea fertilis (Reinw., Blume and Nees) Schiffn. In vitro Culture Mouleidi Dwi Putri; Windri Handayani; Astari Dwiranti; Andi Salamah; Niarsi Merry Hemelda; Afiatry Putrika
HAYATI Journal of Biosciences Vol. 28 No. 1 (2021): January 2021
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.28.1.39

Abstract

In vitro culture gametophytes of leafy liverworts often have problems in their sterilization process. These problems due to the high level of contamination and the fragile structure of the gametophyte leafy liverworts. The structures can be easily to damage after exposure to disinfectant. This study aimed to observe the concentration and the exposure time of “Bayclin” commercial bleach to suppress contamination with the viability of Acrolejeunea fertilisgametophytic explants. This research was conducted using control and 6 combination treatments with “Bayclin” concentration (1.00%, 1.25%, and 1.50%) and exposure time 60 and 120 seconds, then accompanied by the addition of Tetracycline 2.5 mg/ml. The qualitative parameters observed were the explant color, the type and location of contamination, and the growth of explants. The quantitative parameters were the percentage of contamination, the percentage of growth, and the number of new branches. The results showed that “Bayclin” 1.25% and 1.50% with 60 seconds exposure time has the lowest percentage of contamination which is 70% until the 7th days after planting. The most common type of internal contamination from the explant is bacteria and fungi. However, the growth of the new branch still occurs in some explants even though it has been contaminated and browned.
The Development of In Vitro Culture Sterilization Method of Gametophyte Explant Lopholejeunea sp. Anna Widyastuti; Afiatry Putrika; Astari Dwiranti; Andi Salamah; Niarsi Merry Hemelda; Windri Handayani
HAYATI Journal of Biosciences Vol. 28 No. 2 (2021): April 2021
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.28.2.110

Abstract

In vitro cultures of leafy liverworts are still facing significant challenges due to high-level of explant contamination. The sterilization process can easily damage the structure of liverwort after exposure to the disinfectant. This study was to determine the concentration and time exposure of commercial bleach as a disinfectant to suppress contamination using the gametophyte culture of Lopholejeunea sp. The experiment consisted of control and six treatment combinations of commercial bleach with concentration 0.5, 0.75, and 1% (v/v), and exposure time (60 and 90 seconds). The type and location of contamination, the color of the explants after sterilization, and response after 30 days were observed. The results showed that the 0.75% bleach with 60 and 90 seconds exposure time had a lower contamination until the 7th day of culture. The most common type of contamination is bacteria and fungi that arise from the explant. Despite the contamination, it did not inhibit shoot formation. Further studies still needed to determine the type of fungicides and antibiotics with the most potent concentration and exposure time should be tested to obtain an axenic and viable culture of liverworts Lopholejeunea sp.
KOMPOSISI KIMIA, AKTIVITAS ANTIBAKTERI, DAN POTENSI SEBAGAI KEMASAN AKTIF BEBERAPA MINYAK ATSIRI DARI TANAMAN REMPAH INDONESIA Retno Yunilawati; Windri Handayani; Dwinna Rahmi; Aminah Aminah; Cuk Imawan
Jurnal Kimia dan Kemasan Vol. 43 No. 1 April 2021
Publisher : Balai Besar Kimia dan Kemasan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24817/jkk.v43i1.6704

Abstract

Penelitian ini bertujuan untuk  mengetahui komposisi kimia dan aktifitas antibakteri dari 6 jenis minyak atsiri Indoensia. Minyak atsiri  tersebut adalah minyak ketumbar, minyak bunga cengkeh, minyak sereh dapur, minyak kulit jeruk purut, minyak kayu manis, dan minyak kapulaga. Penelitian dilakukan dalam dua tahap yaitu karakterisasi minyak atsiri menggunakan Gas Chromatography Mass Spectrometer (GC-MS) dan penentuan aktivitas antibakteri dari masing-masing minyak atsiri dengan bakteri gram positif Staphylococcus aureus NBRC 100910 dan bakteri gram negatif Escherichia coli NBRC 3301. Aktivitas antibakteri dinyatakan sebagai diameter zona hambat yang terbentuk pada saat pengujian. Minyak ketumbar, minyak sereh dapur dan minyak kayu manis memiliki aktivitas antibakteri  yang kuat  terhadap E. coli NBRC 330 dan S.aureus NBRC 100910 dengan diameter zona hambat pada E. coli NBRC 330 masing-masing sebesar 24 mm, 47 mm dan 34 mm; dan pada S.aureus NBRC 100910 masing masing sebesar 22 mm, 25 mm dan 35 mm. Minyak bunga cengkeh memiliki aktivitas antibakteri dengan tingkat sedang pada E. coli NBRC 330 (19 mm) dan kuat pada S.aureus NBRC 100910 (28 cm). Minyak kapulaga menunjukkan aktivitas antibakteri dengan tingkat sedang  terhadap dua bakeri tersebut dengan diameter zona hambat 14 mm untuk E. coli NBRC 330 dan 12 mm untuk S.aureus NBRC 100910. Minyak kulit jeruk purut hanya memiliki aktivitas antibakteri terhadap S.aureus NBRC 100910 dengan diameter zona hambat sebesar 22 mm. Minyak ketumbar, minyak sereh dapur, dan minyak kayu manis berpotensi besar untuk diaplikasikan dalam memperpanjang umur simpan makanan melalui teknologi kemasan aktif berdasarkan aktivitas antibakterinya.
KOMPOSISI KIMIA, AKTIVITAS ANTIBAKTERI, DAN POTENSI SEBAGAI KEMASAN AKTIF BEBERAPA MINYAK ATSIRI DARI TANAMAN REMPAH INDONESIA Retno Yunilawati; Windri Handayani; Dwinna Rahmi; Aminah Aminah; Cuk Imawan
Jurnal Kimia dan Kemasan Vol. 43 No. 1 April 2021
Publisher : Balai Besar Kimia dan Kemasan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24817/jkk.v43i1.6704

Abstract

Penelitian ini bertujuan untuk  mengetahui komposisi kimia dan aktifitas antibakteri dari 6 jenis minyak atsiri Indoensia. Minyak atsiri  tersebut adalah minyak ketumbar, minyak bunga cengkeh, minyak sereh dapur, minyak kulit jeruk purut, minyak kayu manis, dan minyak kapulaga. Penelitian dilakukan dalam dua tahap yaitu karakterisasi minyak atsiri menggunakan Gas Chromatography Mass Spectrometer (GC-MS) dan penentuan aktivitas antibakteri dari masing-masing minyak atsiri dengan bakteri gram positif Staphylococcus aureus NBRC 100910 dan bakteri gram negatif Escherichia coli NBRC 3301. Aktivitas antibakteri dinyatakan sebagai diameter zona hambat yang terbentuk pada saat pengujian. Minyak ketumbar, minyak sereh dapur dan minyak kayu manis memiliki aktivitas antibakteri  yang kuat  terhadap E. coli NBRC 330 dan S.aureus NBRC 100910 dengan diameter zona hambat pada E. coli NBRC 330 masing-masing sebesar 24 mm, 47 mm dan 34 mm; dan pada S.aureus NBRC 100910 masing masing sebesar 22 mm, 25 mm dan 35 mm. Minyak bunga cengkeh memiliki aktivitas antibakteri dengan tingkat sedang pada E. coli NBRC 330 (19 mm) dan kuat pada S.aureus NBRC 100910 (28 cm). Minyak kapulaga menunjukkan aktivitas antibakteri dengan tingkat sedang  terhadap dua bakeri tersebut dengan diameter zona hambat 14 mm untuk E. coli NBRC 330 dan 12 mm untuk S.aureus NBRC 100910. Minyak kulit jeruk purut hanya memiliki aktivitas antibakteri terhadap S.aureus NBRC 100910 dengan diameter zona hambat sebesar 22 mm. Minyak ketumbar, minyak sereh dapur, dan minyak kayu manis berpotensi besar untuk diaplikasikan dalam memperpanjang umur simpan makanan melalui teknologi kemasan aktif berdasarkan aktivitas antibakterinya.
Karakterisasi Senyawa Volatil dan Uji Antibakteri dari Citrus bergamia dan Citrus sinensis Windri Handayani; Retno Yunilawati
SAINSTECH FARMA Vol 14 No 2 (2021): Sainstech Farma: Jurnal Ilmu Kefarmasian
Publisher : FAKULTAS FARMASI, INSTITUT SAINS DAN TEKNOLOGI NASIONAL

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37277/sfj.v14i2.964

Abstract

Minyak atsiri merupakan bahan organik yang bersifat volatil dan mengandung beberapa konsituen bahan aktif yang memiliki bioaktivitas tertentu. Minyak atsiri dari tanaman jeruk memiliki aroma yang khas dan berbagai kegunaan baik sebagai bahan aditif pada makanan ataupun bahan obat. Pada penelitian ini, minyak atsiri yang berasal dari Citrus bergamia dan Citrus sinensis dikarakterisasi dengan menggunakan kromatografi gas-spektrometri massa (GC-MS). Senyawa yang dominan ditemukan ialah l-limonene yang terkandung C. sinensis, sedangkan pada C. bergamia senyawa dominan ialah Linalool acetate diikuti oleh d-Limonene. Pengujian terhadap aktivitas antibakteri diujikan terhadap bakteri Gram positif Staphylococcus aureus dan bakteri Gram negatif Escherichia coli dengan metode difusi cakram. Hasil yang diperoleh menunjukkan adanya aktivitas penghambatan yang lebih tinggi pada bakteri S. aureus oleh C. bergamia dari pada C. sinensis. Hal tersebut menunjukkan potensi biosprospeksi minyak atsiri dari C. bergamia sebagai agen antibakteri.
Karakteristik Lumut di Ruang Terbuka Hijau (RTH) di Area Permukiman Jakarta Selatan Sarah Tsabituddinillah; Afiatry Putrika; Niarsi Merry Hemelda; Andi Salamah; Windri Handayani; Astari Dwiranti; Mega Atria
Al-Kauniyah: Jurnal Biologi Vol 16, No 1 (2023): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v16i1.21811

Abstract

AbstrakPermukiman merupakan salah satu ruang terbuka hijau (RTH) yang terdapat di daerah urban, khususnya Jakarta. Salah satu kelompok tumbuhan yang ditemui pada RTH tersebut adalah lumut. Keberadaan lumut di permukiman urban menunjukkan adanya kemampuan lumut untuk bertahan pada lingkungan yang terganggu. Penelitian ini bertujuan untuk mengetahui spesies lumut serta karakteristik lumut di salah satu permukiman Jakarta Selatan. Lumut dikoleksi dengan metode transect-line pada 6 titik tepi jalan dan jelajah bebas pada 3 taman di permukiman tersebut Jakarta Selatan. Pengamatan karakteristik morfologi dan anatomi lumut dilakukan dengan penilaian kualitatif dan kuantitatif. Berdasarkan hasil penelitian terdapat 2 divisi lumut, yaitu Bryophyta (lumut sejati) dan Marchantiophyta (lumut hati) di lokasi penelitian. Bryophyta terdiri dari 6 famili, 9 genus, dan 16 spesies. Sementara itu,  Marchantiophyta terdiri dari 2 famili, 2 genus, dan 3 spesies. Pottiaceae merupakan famili dengan jumlah spesies terbanyak ditemukan, yaitu 5 spesies. Fissidens biformis adalah spesies dengan jumlah sampel terbanyak. Lumut tersebut ditemukan pada substrat tanah, batu, dan batang pohon. Kisaran luas tutupan lumut yang ditemukan yaitu 2–100%. Karakteristik seperti ukuran tubuh yang kecil, bentuk hidup, bentuk daun, ornamentasi pada permukaan daun, modifikasi sel daun, serta keberadaan sporofit atau gemma diduga mendukung lumut beradaptasi di lingkungan urban.AbstractSettlement is one of urban green open spaces in Jakarta. One of the plant groups found in the open green spaces is the bryophytes. The presence of bryophytes in the settlement areas indicates the ability of bryophytes to survive in a disturbed environment. This study aims to determine bryophytes species and their characteristic in the settlements area of South Jakarta. Bryophyte collected by transect-line at 6 sites of roadside and broad survey at 3 sites of park. The morphological and anatomical characteristics were observed with qualitative and quantitative assessments. Mosses and liverworts are groups that found in study sites. The mosses consists of 6 families, 9 genera, and 16 species. Meanwhile, the liverworts consists of 2 families, 2 genera, and 3 species. Pottiaceae is has the highest species richness in the location. Meanwhile the highest number of samples was Fissidens biformis. The bryophytes were attached in the soil, rock, and tree trunk. The coverage of bryophyte is about 2–100%. Characteristics such as small body size, life-forms, leaf shape, the ornamentation on the leaf surface, modified leaf cells, and the presence of sporophyte or gemmae are thought to support the adaptation of bryophyte in urban environments.
Karakteristik Lumut di Ruang Terbuka Hijau (RTH) di Area Permukiman Jakarta Selatan Sarah Tsabituddinillah; Afiatry Putrika; Niarsi Merry Hemelda; Andi Salamah; Windri Handayani; Astari Dwiranti; Mega Atria
Al-Kauniyah: Jurnal Biologi Vol 16, No 1 (2023): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v16i1.21811

Abstract

AbstrakPermukiman merupakan salah satu ruang terbuka hijau (RTH) yang terdapat di daerah urban, khususnya Jakarta. Salah satu kelompok tumbuhan yang ditemui pada RTH tersebut adalah lumut. Keberadaan lumut di permukiman urban menunjukkan adanya kemampuan lumut untuk bertahan pada lingkungan yang terganggu. Penelitian ini bertujuan untuk mengetahui spesies lumut serta karakteristik lumut di salah satu permukiman Jakarta Selatan. Lumut dikoleksi dengan metode transect-line pada 6 titik tepi jalan dan jelajah bebas pada 3 taman di permukiman tersebut Jakarta Selatan. Pengamatan karakteristik morfologi dan anatomi lumut dilakukan dengan penilaian kualitatif dan kuantitatif. Berdasarkan hasil penelitian terdapat 2 divisi lumut, yaitu Bryophyta (lumut sejati) dan Marchantiophyta (lumut hati) di lokasi penelitian. Bryophyta terdiri dari 6 famili, 9 genus, dan 16 spesies. Sementara itu,  Marchantiophyta terdiri dari 2 famili, 2 genus, dan 3 spesies. Pottiaceae merupakan famili dengan jumlah spesies terbanyak ditemukan, yaitu 5 spesies. Fissidens biformis adalah spesies dengan jumlah sampel terbanyak. Lumut tersebut ditemukan pada substrat tanah, batu, dan batang pohon. Kisaran luas tutupan lumut yang ditemukan yaitu 2–100%. Karakteristik seperti ukuran tubuh yang kecil, bentuk hidup, bentuk daun, ornamentasi pada permukaan daun, modifikasi sel daun, serta keberadaan sporofit atau gemma diduga mendukung lumut beradaptasi di lingkungan urban.AbstractSettlement is one of urban green open spaces in Jakarta. One of the plant groups found in the open green spaces is the bryophytes. The presence of bryophytes in the settlement areas indicates the ability of bryophytes to survive in a disturbed environment. This study aims to determine bryophytes species and their characteristic in the settlements area of South Jakarta. Bryophyte collected by transect-line at 6 sites of roadside and broad survey at 3 sites of park. The morphological and anatomical characteristics were observed with qualitative and quantitative assessments. Mosses and liverworts are groups that found in study sites. The mosses consists of 6 families, 9 genera, and 16 species. Meanwhile, the liverworts consists of 2 families, 2 genera, and 3 species. Pottiaceae is has the highest species richness in the location. Meanwhile the highest number of samples was Fissidens biformis. The bryophytes were attached in the soil, rock, and tree trunk. The coverage of bryophyte is about 2–100%. Characteristics such as small body size, life-forms, leaf shape, the ornamentation on the leaf surface, modified leaf cells, and the presence of sporophyte or gemmae are thought to support the adaptation of bryophyte in urban environments.
Comparative Study of The Physiological Condition of Six Tree Species to Air Pollution in Depok City, West Java Ajenk Ayunda; Ratna Yuniati; Windri Handayani
BIOEDUSCIENCE Vol 7 No 2 (2023): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/9749

Abstract

Background: Air pollution is a threat to the environment. Sources of air pollutants in urban environments can be in the form of dust, heavy metals, and hydrocarbons. Plants can help clean air pollutants from the atmosphere by absorption through the stomata, accumulating them, or by adsorption on the leaf surface. The Air Pollution Tolerance Index (APTI) is used as an evaluation benchmark for the Physiological conditions of plants exposed to air pollution. This research aims to study the physiological conditions of six tree species in air conditions in the Depok City area and to assess the plants' tolerance level based on the APTI calculation. Methods: The physiological parameters measured to calculate APTI were ascorbic acid, total chlorophyll, leaf extract pH, and Relative Water Content (RWC). The six tree species used as objects in this study were Artocarpus altilis, Artocarpus heterophyllus, Bauhinia purpurea, Ficus septica, Filicium decipiens, and Nephelium lappaceum. Results: Differences in the physiological conditions of six tree species in the Depok City area based on the average ascorbic acid values, total chlorophyll, leaf extract pH, and RWC. In addition, there are also differences in APTI parameters between the two research locations. Conclusions: Filicium decipiens is the plant species with the highest APTI, while Artocarpus heterophyllus has the lowest. Based on the APTI scores, Bauhinia purpurea, Ficus septica, Filicium decipiens, Nephelium lappaceum belong to moderately tolerant category, Artocarpus altilis belongs to an intermediate category, Artocarpus heterophyllus belongs to sensitive category.
Plukenetia volubilis L.: A New Record of a Cultivated Alien Species in Java Tianara, Alexander; Handayani, Windri; Irsyam, Arifin Surya Dwipa; Hariri, Muhammad Rifqi; Dewi, Asih Perwita; Peniwidiyanti, Peniwidiyanti; Baidlowi, Muhammad Hisyam; Rosleine, Dian; Atria, Mega
Journal of Tropical Biodiversity and Biotechnology Vol 9, No 1 (2024): March
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.84523

Abstract

Plukenetia volubilis L. has been documented as a new record for the first time in Java, Indonesia. The species is easily distinguished from the native species, P. corniculata Sm., by its exstipellate basilaminar-glands, long cylindrical column, and wingless fruit-lobes. Plukenetia volubilis is cultivated mainly in South America for its beneficial values as food and medicine and was recently introduced to Asia. However, its occurrence in Java has not been reported. We collected specimens from West Java (Depok City, Bandung Barat and Sumedang Regency) and East Java (Malang Regency). Morphological description, identification key, and photographs of the species are provided.
Stem-base Rot Disease Detection in Oil Palm using RGB (Red, Green, Blue) and OCN (Orange, Cyan, NIR) Image Fusion Method Based on ResNet50 Panggabean, Prima Ria Rumata; Rista, Rista; Saputro, Adhi Harmoko; Handayani, Windri
Spektra: Jurnal Fisika dan Aplikasinya Vol. 10 No. 1 (2025): SPEKTRA: Jurnal Fisika dan Aplikasinya, Volume 10 Issue 1, April 2025
Publisher : Program Studi Fisika Universitas Negeri Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21009/SPEKTRA.101.02

Abstract

Current image acquisition and processing methods still need to be improved to effectively detect oil palm diseases. A precise and fast method to detect stem base rot disease in oil palm trees can be developed using drone technology and image processing approaches. An OCN (Orange, Cyan, NIR) camera is added to a standard drone and equipped with an RGB (Red, Green, Blue) camera. Combining the two cameras is proposed to generate multispectral imagery using an image fusion method called early fusion. A Multispectral Convolution Neural Network (MCNN) is also introduced to detect stem base rot disease by analysing the leaf patterns of oil palms. Healthy and unhealthy leaf samples were collected from oil palm plantations in Bogor. The images that have passed the image processing stage with the fusion method become inputs for modelling to identify stem base rot disease in oil palm. The results of the research using the multispectral image fusion method (RGB and OCN) based on the ResNet50 architecture can be used to identify stem base rot disease in oil palm effectively, as evidenced by the training and validation accuracy of 97.75% and 96.48%.
Co-Authors ABDULLAH RASYID Adhi Harmoko Saputro Aini, Fadita Nurul Ajenk Ayunda Aminah Aminah Aminah Umar Andi Salamah Anna Widyastuti ASIH PERWITA DEWI, ASIH PERWITA Astari Dwiranti Ayunda, Ajenk Baidlowi, Muhammad Hisyam Cuk Imawan Cynthia Putri Yuwana Dian Rosleine, Dian Dwinna Rahmi HALIMAH HALIMAH Irsyam, Arifin Surya Dwipa ITA KHOERUNNISA LAELIA NUGRAHANI Lutfan Alharits Maryenti, Tety MASTERIA YUNOVILSA PUTRA Mega Atria Mouleidi Dwi Putri Muhammad Rifqi Hariri Munawarohthus, Sholikha Niarsi Merry Hemelda Nissa, Maftucha Panggabean, Prima Ria Rumata Peniwidiyanti, Peniwidiyanti Putrika, Afiatry Ramadhani, Nadhifa Tazkia RATNA YUNIATI Ratna Yuniati Ratna Yuniati Retno Yunilawati Retno Yunilawati Richard Owen Tanadi Rika, Amelia Rista, Rista Sarah Tsabituddinillah Syafriana, Vilya Tianara, Alexander Upi Chairun Nisa Yasman Yasman Yuniati, Ratna Yuwana, Cynthia Putri