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All Journal Agrointek RECHTSTAAT Indonesian Food Science and TechnologyJournal Jurnal Teknologi & Industri Hasil Pertanian AGRITEKNO, Jurnal Teknologi Pertanian Jurnal Ilmiah Inovasi Jurnal Agrinika : Jurnal Agroteknologi dan Agribisnis
Giyarto Giyarto
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Education Industrial & Manufacturing Engineering Other

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Journal : Indonesian Food Science and TechnologyJournal

 1 
Development of Enzyme Production Technology with Double Protease Immobilization Method (Calotropin and Papain) Witono, Yuli; Masahid, Ardiyan Dwi; Giyarto, Giyarto; Belgis, Maria; Jayanti, Puja Dwi; Wahyuni, Livia
Indonesian Food Science and Technology Journal Vol. 8 No. 2 (2025): Volume 8. Number 2, July 2025 |IFSTJ|
Publisher : Department of Technology of Agricultural product (THP) Jambi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/ifstj.v8i2.37509

Abstract

Abstract—Enzymes are biocatalysts widely applied in agriculture, food processing, chemical industries, and medical fields. Enzymes can be used singly or immobilized within a matrix. However, free enzymes often exhibit lower stability compared to immobilized enzymes. Therefore, this research aimed to evaluate the combined two proteolytic enzymes for extracting protein hydrolysates. The objective was to analyze enzymes' immobilization characteristics using carrageenan and determine the optimal concentration combination. Calotropin and papain were applied at concentrations of 2.5 mL, 3.5 mL, and 4.0 mL, respectively. The analysis parameters included yield, enzyme activity, soluble protein content, specific enzyme activity, stability, and kinetic parameters (Km and Vmax). The results showed that the combined proteases exhibited yields ranging from 44.43% to 48.63%; enzyme activities of 9.23–53.63 U/mL; soluble protein contents of 2.40–2.89 mg/mL; and specific enzyme activities of 3.54–18.62 U/mg protein. The enzymes were maintained stability at temperatures ranging from 30 to 60 °C. The kinetic parameters were determined as Km = 0.0034 g/mL and Vmax = 17.094 U/min. 
Co-Authors Achmad Subagio Adilah, Hamasah Agafatmi, Izzun Ahmad Nafi Azkiyah, Lailatul Indarto Indarto Irsyam, Mahrus Jannah, Sulihati Jayanti, Puja Dwi Lestari, Ning Puji Maria Belgis Masahid, Ardiyan Dwi Mukhammad Fauzi Ngizati, Naila Alfi Niken Widya Palupi Nur Aini Nurud Diniyah Rina Dias Agustin Rusdianto, Andrew S Wahyuni, Livia Yuli Witono