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KERAGAAN TANAMAN KAKAO ASAL EMBRIO SOMATIK DI LAPANGAN NUR AJIJAH; ENNY RANDRIANI; RUBIYO RUBIYO; DEWI SUKMA; SUDARSONO SUDARSONO
Jurnal Penelitian Tanaman Industri Vol 21, No 2 (2015): Juni 2015
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v21n2.2015.57-68

Abstract

ABSTRAKSekitar 75 juta bibit kakao asal embrio somatik (ES) telah ditanam di lapangan.  Evaluasi keragaan tanaman tersebut perlu terus dilakukan. Penelitian yang bertujuan untuk mengevaluasi keragaan tanaman kakao asal ES di lapangan telah dilaksanakan di delapan lokasi di Kabupaten Soppeng, Sulawesi Selatan dan kabupaten Mamuju (Sulawesi Barat) pada bulan Juni 2012 sampai  Desember 2013. Pengamatan dilakukan terhadap tanaman umur 1,5-4 tahun meliputi tinggi jorget, lingkar batang, jumlah cabang primer, persentase tanaman berbunga, persentase tanaman berbuah, jumlah buah per pohon, jumlah biji per buah, bobot kering biji serta kejadian serangan hama dan penyakit. Tanaman kakao asal ES memiliki arsitektur   seperti   tanaman   yang   berasal   dari   biji,   yaitu memiliki pertumbuhan  dimorfik  dan  membentuk  jorget.  Rataan  tinggi  jorget bervariasi antar lokasi, sebagian besar berkisar 100 -150 cm. Di lapangan ditemukan tanaman asal ES dengan tinggi jorget > 2 m. Perlu dievaluasi lebih lanjut apakah tinggi jorget > 2 m merupakan bentuk penyimpangan atau hanya pengaruh faktor lingkungan. Pada saat dilakukan pengamatan, tanaman kakao asal ES yang dievaluasi sudah berbunga dan berbuah dengan persentase berbunga 34-100%, berbuah 16-100%, dan dengan hasil buah per pohon 6-37 buah. Bobot buah yang dipanen berkisar 277-418 g dengan rataan jumlah biji per buah 43 biji dan bobot kering per biji 0.5 -1.4 g. Kejadian serangan penggerek buah di 3 lokasi pengamatan mencapai 82, 34.8 dan 49.6%, sedangkan busuk buah 2, 4.3 dan 18%. Studi lebih lanjut  diperlukan  untuk  memastikan  bahwa  tanaman  kakao  asal  ES mempunyai karakteristik tanaman dan hasil yang tidak berbeda dengan tanaman kakao  asal bibit tradisional yang telah biasa digunakan petani.Kata kunci:  Theobroma   cacao   L.,   bibit   ES,   perbanyakan   masal, pertumbuhan dan komponen hasil, variasi somaklonal Field Performance of Cacao Somatic Embryos Derived PlantsABSTRACTApproximately 75 millions of cacao seedlings propagated through somatic embryogenesis (SE) have been planted in the field. Evaluation of the performance of those SE derived plants needs to be continued. The research aimed to evaluate the performance of the cacao SE derived plants in the field have been implemented in 8 locations in Soppeng district, South Sulawesi and the Mamuju district, West Sulawesi in June 2012 and December 2013.   Collected   data   include:   jorquette   height,   trunk circumference,  jorquette branch  numbers,  flowering  and  fruiting tree percentages, fruit numbers per tree, pod weight, bean number per pod, bean dry weight and the disease and pest infection rates. Cacao plants derived from SE showed similar plant architectures to those of seed derived ones, such as having dimorphic growth and forming jorquette. The jorquette height of the majority of cacao trees in the evaluated regionsranged from 100-150 cm. A few SE derived cacao trees show jorquette height > 2 m. However, it needs further studies to determine whether they indicate either abnormality or environment effects. The SE derived cacao trees in the studied locations has bear flowers and fruits. The percentages of cacao trees with flowers or fruits at the age of 2.5 years ranged from 34-100% or 16-100%, respectively. The observed pods number per tree was 6-37 pods, the pod weight was 277-418 g, the beans number per pod was 42-43  and the bean dry weight was 0.5 -1.4 g. The incidences of fruit borer infection at the three evaluated locations were 82, 34.8 dan 49.6%, while the incidences of black pod infection were 2, 4.3 dan 18%. To conclude whether the SE derived cacao trees are comparable to those of the traditional cacao planting materials  requires  more comprehensive studies.Keywords:  Theobroma cacao L., SE derived seedling, mass propagation, growth and yield components, somaclonal variation
Pengaruh Tipe Kultur Kontainer pada Keberhasilan Pembentukan Embrio Somatik Kakao Nur Ajijah; Cici Tresniawati; Syafaruddin Syafaruddin
Jurnal Tanaman Industri dan Penyegar Vol 6, No 2 (2019): Jurnal Tanaman Industri dan Penyegar
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jtidp.v6n2.2019.p89-98

Abstract

Container culture have an important role in determining the success of in vitro culture since it will affect the development of culture, such as the formation of embryonic structures. The study aimed to determine the effect of culture container types on cacao somatic embryogenesis. The study was conducted at Tissue Culture Laboratory, Superior Seed Development Unit of IAARD, Bogor, from April to September 2016. The tests were conducted on the effect of container and explant types as well as the effect of container types and genotypes. The effects of container and explant types were tested using callus induced from petal and staminoid explants of Sca 6, whereas the effects of container types and genotypes were tested using callus induced from petal explants of Sca 6 and ICCRI 4. Afterwards, the somatic embryos were induced using petri dishes or culture bottles according to treatment. The results showed no significant interaction between container and explant types on the average percentage of the formation and number of somatic embryos (10.28% embryos/explants in culture bottles and 7.89% embryos/explants in petri dishes). Meanwhile, there was significant interaction between genotypes and container types in the initial period of somatic embryos formation (15 and 18 weeks after culture), but the effect was not significant in the final period of observation (21 weeks after culture). The results indicate that culture bottles, which have lower prices, can be used to replace petri dishes to induce the formation of somatic embryos in cacao.
Peningkatan Efisiensi Media Pembentukan Embrio Somatik Kakao Melalui Penggunaan Gula Pasir Nur Ajijah; Sri Hartati
Jurnal Tanaman Industri dan Penyegar Vol 7, No 1 (2020): Jurnal Tanaman Industri dan Penyegar
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jtidp.v7n1.2020.p29-38

Abstract

Availability of inexpensive and efficient tissue culture methods is needed in the development of mass propagation methods. The efficiency can be obtained through the use of low cost media components such as granulated sugar. The study aimed to determine the efficiency of cacao somatic embryo development medium using granulated sugar and its effect on somatic embryo formation. The study was conducted at Tissue Culture Laboratory for Superior Seed Development, IAARD, Bogor, from March to October 2016. Formation and maturation of somatic embryos was conducted using DKW medium without growth regulators. The treatments tested were carbon sources in the media (laboratory grade sucrose; a mixture of sucrose and granulated sugar with a ratio of 3:1, 2:2 and 1:3; granulated sugar) and cacao genotypes (Sca 6 and ICCRI 4). The study used in a randomized complete block design in factorial 2 favtors with 8 replications, which consisted of 10 explants per experimental unit. The results showed that carbon sources and genotypes significantly affected somatic embryos formation, whereas the interaction effect was not significant. The use of granulated sugar 30 g/l produced approximately 35.3% of somatic embryo formation and 3.5 somatic embryos per explant, which is not significantly different from sucrose 30 g/l (31.1% and 4.1), whereas the mixture of sucrose and granulated sugar produced the lowest average percentage of somatic embryo formation and the number of somatic embryos per explant (20.3%, 18.8%, 17.8% and 1.5; 2.2; 2.8 respectively). The low-cost somatic embryogenesis method was successfully developed with an efficiency of 98.8% per liter of media.
Pengaruh Sitokinin, Jenis Eksplan, dan Genotipe terhadap Embriogenesis Somatik Kakao Nur Ajijah; RR. Sri Hartati
Jurnal Tanaman Industri dan Penyegar Vol 3, No 2 (2016): Jurnal Tanaman Industri dan Penyegar
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jtidp.v3n2.2016.p71-82

Abstract

Information on the effect of cytokinins on cacao (Theobroma cacao L.) primary somatic embryogenesis and its interaction with explant types and genotypes is not yet known. This study aimed to evaluate the effect of cytokinins and its interaction with explant types and genotypes on cacao somatic embryogenesis. The study was conducted at tissue culture laboratory of IAARD, Bogor from April until December 2012 and October 2014 until February 2016. Three types of cytokinins i.e. kinetin (0.58, 1.16, and 2.32 μM), thidiazuron (0.01, 0.02, and 0.04 μM) and benzylaminopurine (0.55, 1.11, and 2.22 μM) in combination with 9 μM 2,4-D were tested for their effectiveness in inducing somatic embryogenesis from petals and staminoid explants of Cimanggu 1 genotype. Furthermore, three levels of kinetin (0.58, 1.16, and 2.32 μM) also in combination with 9 μM 2,4-D were evaluated for their influences on the somatic embryogenesis from petals and staminoid explants of three cacao genotypes i.e. Sulawesi 02, ICCRI 04 and Cimanggu 3. The result demonstrated that 2.32 μM kinetin and staminoids explant were more effective to induce cacao somatic embryogenesis of Cimanggu 1 genotype (7%, 0.23 embryos/explant). Additionally, there were interaction effects between the level of kinetin with explant types and genotype on the percentage of explants forming embryo at 12 weeks after culture. The highest percentage of somatic embryo formation was shown by ICCRI 04 genotype with the use of petals explant and a kinetin level of 1.16 μM (31.85%), but not significantly different from the level of kinetin 2.23 μM (25.55%). The formation of primary somatic embryos of cacao is largely determined by the type and level of cytokinins, type of explant, and genotype.
KERAGAAN TANAMAN KAKAO ASAL EMBRIO SOMATIK DI LAPANGAN NUR AJIJAH; ENNY RANDRIANI; RUBIYO RUBIYO; DEWI SUKMA; SUDARSONO SUDARSONO
Jurnal Penelitian Tanaman Industri Vol 21, No 2 (2015): Juni 2015
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (389.687 KB) | DOI: 10.21082/jlittri.v21n2.2015.57-68

Abstract

ABSTRAKSekitar 75 juta bibit kakao asal embrio somatik (ES) telah ditanam di lapangan.  Evaluasi keragaan tanaman tersebut perlu terus dilakukan. Penelitian yang bertujuan untuk mengevaluasi keragaan tanaman kakao asal ES di lapangan telah dilaksanakan di delapan lokasi di Kabupaten Soppeng, Sulawesi Selatan dan kabupaten Mamuju (Sulawesi Barat) pada bulan Juni 2012 sampai  Desember 2013. Pengamatan dilakukan terhadap tanaman umur 1,5-4 tahun meliputi tinggi jorget, lingkar batang, jumlah cabang primer, persentase tanaman berbunga, persentase tanaman berbuah, jumlah buah per pohon, jumlah biji per buah, bobot kering biji serta kejadian serangan hama dan penyakit. Tanaman kakao asal ES memiliki arsitektur   seperti   tanaman   yang   berasal   dari   biji,   yaitu memiliki pertumbuhan  dimorfik  dan  membentuk  jorget.  Rataan  tinggi  jorget bervariasi antar lokasi, sebagian besar berkisar 100 -150 cm. Di lapangan ditemukan tanaman asal ES dengan tinggi jorget > 2 m. Perlu dievaluasi lebih lanjut apakah tinggi jorget > 2 m merupakan bentuk penyimpangan atau hanya pengaruh faktor lingkungan. Pada saat dilakukan pengamatan, tanaman kakao asal ES yang dievaluasi sudah berbunga dan berbuah dengan persentase berbunga 34-100%, berbuah 16-100%, dan dengan hasil buah per pohon 6-37 buah. Bobot buah yang dipanen berkisar 277-418 g dengan rataan jumlah biji per buah 43 biji dan bobot kering per biji 0.5 -1.4 g. Kejadian serangan penggerek buah di 3 lokasi pengamatan mencapai 82, 34.8 dan 49.6%, sedangkan busuk buah 2, 4.3 dan 18%. Studi lebih lanjut  diperlukan  untuk  memastikan  bahwa  tanaman  kakao  asal  ES mempunyai karakteristik tanaman dan hasil yang tidak berbeda dengan tanaman kakao  asal bibit tradisional yang telah biasa digunakan petani.Kata kunci:  Theobroma   cacao   L.,   bibit   ES,   perbanyakan   masal, pertumbuhan dan komponen hasil, variasi somaklonal Field Performance of Cacao Somatic Embryos Derived PlantsABSTRACTApproximately 75 millions of cacao seedlings propagated through somatic embryogenesis (SE) have been planted in the field. Evaluation of the performance of those SE derived plants needs to be continued. The research aimed to evaluate the performance of the cacao SE derived plants in the field have been implemented in 8 locations in Soppeng district, South Sulawesi and the Mamuju district, West Sulawesi in June 2012 and December 2013.   Collected   data   include:   jorquette   height,   trunk circumference,  jorquette branch  numbers,  flowering  and  fruiting tree percentages, fruit numbers per tree, pod weight, bean number per pod, bean dry weight and the disease and pest infection rates. Cacao plants derived from SE showed similar plant architectures to those of seed derived ones, such as having dimorphic growth and forming jorquette. The jorquette height of the majority of cacao trees in the evaluated regionsranged from 100-150 cm. A few SE derived cacao trees show jorquette height > 2 m. However, it needs further studies to determine whether they indicate either abnormality or environment effects. The SE derived cacao trees in the studied locations has bear flowers and fruits. The percentages of cacao trees with flowers or fruits at the age of 2.5 years ranged from 34-100% or 16-100%, respectively. The observed pods number per tree was 6-37 pods, the pod weight was 277-418 g, the beans number per pod was 42-43  and the bean dry weight was 0.5 -1.4 g. The incidences of fruit borer infection at the three evaluated locations were 82, 34.8 dan 49.6%, while the incidences of black pod infection were 2, 4.3 dan 18%. To conclude whether the SE derived cacao trees are comparable to those of the traditional cacao planting materials  requires  more comprehensive studies.Keywords:  Theobroma cacao L., SE derived seedling, mass propagation, growth and yield components, somaclonal variation