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KERAGAAN TANAMAN KAKAO ASAL EMBRIO SOMATIK DI LAPANGAN NUR AJIJAH; ENNY RANDRIANI; RUBIYO RUBIYO; DEWI SUKMA; SUDARSONO SUDARSONO
Jurnal Penelitian Tanaman Industri Vol 21, No 2 (2015): Juni 2015
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v21n2.2015.57-68

ABSTRAKSekitar 75 juta bibit kakao asal embrio somatik (ES) telah ditanam di lapangan. Evaluasi keragaan tanaman tersebut perlu terus dilakukan. Penelitian yang bertujuan untuk mengevaluasi keragaan tanaman kakao asal ES di lapangan telah dilaksanakan di delapan lokasi di Kabupaten Soppeng, Sulawesi Selatan dan kabupaten Mamuju (Sulawesi Barat) pada bulan Juni 2012 sampai Desember 2013. Pengamatan dilakukan terhadap tanaman umur 1,5-4 tahun meliputi tinggi jorget, lingkar batang, jumlah cabang primer, persentase tanaman berbunga, persentase tanaman berbuah, jumlah buah per pohon, jumlah biji per buah, bobot kering biji serta kejadian serangan hama dan penyakit. Tanaman kakao asal ES memiliki arsitektur seperti tanaman yang berasal dari biji, yaitu memiliki pertumbuhan dimorfik dan membentuk jorget. Rataan tinggi jorget bervariasi antar lokasi, sebagian besar berkisar 100 -150 cm. Di lapangan ditemukan tanaman asal ES dengan tinggi jorget > 2 m. Perlu dievaluasi lebih lanjut apakah tinggi jorget > 2 m merupakan bentuk penyimpangan atau hanya pengaruh faktor lingkungan. Pada saat dilakukan pengamatan, tanaman kakao asal ES yang dievaluasi sudah berbunga dan berbuah dengan persentase berbunga 34-100%, berbuah 16-100%, dan dengan hasil buah per pohon 6-37 buah. Bobot buah yang dipanen berkisar 277-418 g dengan rataan jumlah biji per buah 43 biji dan bobot kering per biji 0.5 -1.4 g. Kejadian serangan penggerek buah di 3 lokasi pengamatan mencapai 82, 34.8 dan 49.6%, sedangkan busuk buah 2, 4.3 dan 18%. Studi lebih lanjut diperlukan untuk memastikan bahwa tanaman kakao asal ES mempunyai karakteristik tanaman dan hasil yang tidak berbeda dengan tanaman kakao asal bibit tradisional yang telah biasa digunakan petani.Kata kunci: Theobroma cacao L., bibit ES, perbanyakan masal, pertumbuhan dan komponen hasil, variasi somaklonal Field Performance of Cacao Somatic Embryos Derived PlantsABSTRACTApproximately 75 millions of cacao seedlings propagated through somatic embryogenesis (SE) have been planted in the field. Evaluation of the performance of those SE derived plants needs to be continued. The research aimed to evaluate the performance of the cacao SE derived plants in the field have been implemented in 8 locations in Soppeng district, South Sulawesi and the Mamuju district, West Sulawesi in June 2012 and December 2013. Collected data include: jorquette height, trunk circumference, jorquette branch numbers, flowering and fruiting tree percentages, fruit numbers per tree, pod weight, bean number per pod, bean dry weight and the disease and pest infection rates. Cacao plants derived from SE showed similar plant architectures to those of seed derived ones, such as having dimorphic growth and forming jorquette. The jorquette height of the majority of cacao trees in the evaluated regionsranged from 100-150 cm. A few SE derived cacao trees show jorquette height > 2 m. However, it needs further studies to determine whether they indicate either abnormality or environment effects. The SE derived cacao trees in the studied locations has bear flowers and fruits. The percentages of cacao trees with flowers or fruits at the age of 2.5 years ranged from 34-100% or 16-100%, respectively. The observed pods number per tree was 6-37 pods, the pod weight was 277-418 g, the beans number per pod was 42-43 and the bean dry weight was 0.5 -1.4 g. The incidences of fruit borer infection at the three evaluated locations were 82, 34.8 dan 49.6%, while the incidences of black pod infection were 2, 4.3 dan 18%. To conclude whether the SE derived cacao trees are comparable to those of the traditional cacao planting materials requires more comprehensive studies.Keywords: Theobroma cacao L., SE derived seedling, mass propagation, growth and yield components, somaclonal variation

Stabilitas Hasil Tiga Klon Kopi Robusta Bengkulu sebagai Klon Unggul Lokal Enny Randriani; Edi Wardiana
Jurnal Tanaman Industri dan Penyegar Vol 2, No 3 (2015): Jurnal Tanaman Industri dan Penyegar
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jtidp.v2n3.2015.p159-168

The population of Robusta coffee in Bengkulu province were mostly old thus it need rejuvenation or rehabilitation. Local clones of Robusta coffee, such as Sidodadi, Payung Hijau and Payung Kuning, have been cultivated for rehabilitation in Bengkulu Province at an altitude of >700 m above sea level (asl). This study aimed to analyze the yield stability of the three Robusta coffee clones at medium to high altitudes. The study was conducted from January to September 2015 in Curup Regency (670 m asl) and Kepahiang Regency (900 m and 1300 m asl), Bengkulu Province. The three tested Robusta coffee clones were 5 years old Sidodadi, Payung Hijau, and Payung Kuning,which propagated through plagiotropic grafting. Each experiment unit consisted of 5 plant samples and were obtained by randomized sampling with 5 replications respectively. The parameters observed were fresh weight of berry/plant, dry weight of beans/plant, fresh weight/berry, and dry weight/beans. The data analyzed using combined variance followed by yield stability test of Eberhart and Russell method. The results showed that Payung Hijau clone had the highest bean yield compared to the other two clones (Sidodadi and Payung kuning). The production of Payung Hijau clone reached 1.19 kg dry weight of bean/plant, 4.31 g fresh weight/berry, and 0.63 g dry weight/bean. Therefore, the Payung Hijau clone was potentially used as scions source for the coffee plantation rejuvenation or rehabilitation.

Pengaruh Iradiasi Sinar Gamma terhadap Pertumbuhan Stek Tunas Apikal dan Aksilar Kopi Arabika Meynarti Sari Dewi Ibrahim; Enny Randriani
Jurnal Tanaman Industri dan Penyegar Vol 7, No 3 (2020): Jurnal Tanaman Industri dan Penyegar
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jtidp.v7n3.2020.p137-148

The Arabica coffee is predominantly self-pollinated plants thereby contributing to low genetic diversity. The effort to increase the genetic diversity of Arabica coffee through crossing strategy is time-consuming, and induce mutation is necessary to enhance the rate of genetic variation. The aims of this study were to observe the effect of gamma-ray irradiation on the growth of apical and axillary bud cuttings and to determine the value of LD50 on apical cuttings and Arabica coffee axillaries. The study was conducted at the Tissue Culture Laboratory, Industrial and Beverage Crops Research Institute, from January to December in 2018.. The planting material that was irradiated was Arabica coffee plantlets resulting from somatic embryogenesis propagation. Irradiation is carried out at the National Nuclear Energy Agency. The irradiated plantlets were cut and subcultured onto MS medium without growth regulators, 30 g L-1 sucrose, and 2.5 g L-1 phytagel were added. The design used a completely randomized design with 10 replications. The treatments tested were the dose of gamma-ray irradiation (0, 10, 20, 30, 40, and 50 Gy). The results showed that gamma-ray irradiation had an effect on all observed parameters. The mortality percentage of apical shoot cuttings began to be found at 30 Gy, while axillary cuttings at 20 Gy increased with an increasing dose of gamma-ray irradiation. The number of shoots and leaves varied between irradiation doses on both apical and axillary cuttings. The LD50 value of apical shoot cuttings was 36.80 Gy, while axillary cuttings were 22.24 Gy

Efisiensi Teknis Usaha Tani Kopi Arabika Garut Kuning (AGK) Bedy Sudjarmoko; Enny Randriani
Jurnal Tanaman Industri dan Penyegar Vol 6, No 1 (2019): Jurnal Tanaman Industri dan Penyegar
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jtidp.v6n1.2019.p13-20

Coffee is one of the strategic commodities in West Java for its important economic role in West Java. However, studies on coffee farming efficiency are still rarely found. Information on efficiency is critical because it affects the benefits the coffee farmers gain and its development program. This study aimed to investigate factors affecting the technical efficiency and technical efficiency level of Arabica Garut Kuning (AGK) coffee farming in Garut Regency, carried out from June to September 2016 using survey methods. Research location was determined by purposive sampling and 72 respondents were randomly chosen from Arabica coffee farmer population in Garut Regency. The data was analysed using stochastic frontier production, estimated by Maximum Likelihood Estimates (MLE) method. The results showed that the factors influencing the technical efficiency of coffee farming are land area, use of Urea fertilizer, ZA fertilizer, SP 36 fertilizer, herbicide and labor. The technical efficiency of farmers is quite high (average of 0.81). Means farmers have been technically efficient especially in allocating resources and utilizing existing cultivation technology. To further improve the technical efficiency as well as its revenue share of AGK, support from local governments and other stakeholders is still urgently needed.

Pemetaan Atribut Sensori Kopi Kobura Berdasarkan Perbedaan Cara Panen dan Pengolahan di Tingkat Petani Enny Randriani; Elsera Br Tarigan; Edi Wardiana
Jurnal Tanaman Industri dan Penyegar Vol 8, No 3 (2021): Jurnal Tanaman Industri dan Penyegar
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jtidp.v8n3.2021.p109-120

In general, Kobura coffee farmers in South Ogan Komering Ulu (OKU) harvest coffee with the criteria of red fruit and a mixture of red and green, while the processing method is a dry process. However, the sensory attributes of these activities are not yet known. This study aims to map the sensory qualities of Kobura coffee at the farmer level based on differences in harvesting and processing methods. The research was carried out in Banding Agung District, South OKU Regency, and the Coffee and Cocoa Bioindustry Laboratory, Balittri, Sukabumi, from March to December 2019. The design used was a completely randomized design with 3 treatment factors and two replications. The first factor was three clones of Kobura coffee (Kobura 1, 2, 3), the second factor was two harvesting methods (red and mix of red + green fruit), and the third factor is two processing methods (dry and wet). The variable observed were the sensory attributes analyzed by multivariate analysis of variance, principal component analysis, cluster analysis, and path analysis. The results showed that the harvesting and processing methods of Kobura coffee at the farmer level in South OKU did not significantly affect the sensory. Furthermore, each cluster's harvesting and processing methods were evenly distributed because the proportion between red and green fruit was not clearly identified in the mixed fruit harvest. Superior genetic and optimal altitude factors make Kobura coffee categorized into Fine Robusta with a final score of 81.13-85.75. The final score is directly and dominantly influenced by mouthfeel (body), flavor, overall, and fragrance, respectively.

Analisis Kekerabatan Genetik Kultivar Kopi Arabika Berbuah Kuning dan Berbuah Merah Berdasarkan Marka SSR Nur Kholilatul Izzah; Enny Randriani; Dani Dani
Jurnal Tanaman Industri dan Penyegar Vol 2, No 3 (2015): Jurnal Tanaman Industri dan Penyegar
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jtidp.v2n3.2015.p113-122

Yellow berry of Arabica coffee (AGK-1) cultivated in Garut is a local cultivar that potentially could be released as a superior variety. However, its genetic background has not been studied. Information of genetic background is one of the requirement in releasing of a new variety. The objective of this research was to analyze the genetic relationships of AGK-1 cultivar with 11 red berries Arabica coffee cultivars based on simple sequence repeats (SSR) markers to find polymorphisms and clustering. The research was carried out at the Molecular Laboratory of Indonesian Industrial and Beverage Crops Research Institute and Biology Molecular Laboratory of Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development, from January to March 2015. These twelve Arabica coffee cultivars were obtained from Garut Regency. A total of 12 SSR primers were used to investigate the genetic relationships of the plant. The result showed that 12 SSR markers were adequate to identify the relationships among 12 Arabica coffee cultivars. The genetic clustering obtained in this study is related to plant morphology, particularly plant growth characters, such as tall, semi dwarf and dwarf. AGK-1 cultivar genetically related to ABP-2, one of red berries cultivars that originated from Brazil. Both of these cultivars have the same growing type characters (i.e. semi dwarf). AGK-1 cultivar that has yellow berry color presumably derived from an open pollinated red berries parents, mainly ABP-2 cultivar. Therefore, AGK-1 is a unique cultivar that could be released as a local superior variety.

Ketahanan 13 Nomor Koleksi Plasma Nutfah Jambu Mete terhadap Penyakit Busuk Akar Fusarium Dani Dani; Efi Taufiq; Handi Supriadi; Enny Randriani; Ilham Nur Ardhi Wicaksono
Jurnal Tanaman Industri dan Penyegar Vol 3, No 2 (2012): Buletin Riset Tanaman Rempah Dan Aneka Tanaman Industri
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jtidp.v3n2.2012.p171-176

Busuk akar Fusarium merupakan salah satu penyakit penting yang menyerang tanaman jambu mete pada fase bibit maupun dewasa. Oleh sebab itu, upaya perakitan varietas tanaman jambu mete yang tahan terhadap serangan penyakit tersebut perlu dilakukan. Tujuan penelitian adalah menguji ketahanan nomor-nomor koleksi plasma nutfah jambu mete terhadap serangan penyakit busuk akar Fusarium. Seleksi dilakukan terhadap bibit hasil persarian terbuka dari 13 nomor aksesi jambu mete koleksi plasma nutfah di Kebun Percobaan (KP) Cikampek. Penelitian dilaksanakan pada fase bibit di dalam rumah plastik dengan naungan paranet intensitas 50% di KP Pakuwon. Perlakuan disusun dalam Rancangan Acak Kelompok dengan tiga ulangan. Inokulasi buatan menggunakan isolat Fusarium sp. dari tanah yang dicairkan sampai kepadatan konidia 108 konidia/ml. Hasil pengamatan menujukkan bahwa seluruh nomor aksesi jambu mete yang diuji tidak tahan terhadap serangan penyakit busuk akar Fusarium. Persentase kejadian penyakit paling tinggi ditunjukkan oleh nomor aksesi Lembor 2, M Z Lux, dan Ekoae Kecil, yaitu mencapai 93,33%, sedangkan aksesi JN 26 menunjukkan persentase kejadian sebesar 63,33%. Tingkat keparahan penyakit paling tinggi ditunjukkan oleh nomor aksesi Menini 15, yaitu mencapai 83,56%, meskipun secara statistik tidak berbeda nyata dengan Kodi 2 dan Kobawani yang masing-masing 82,92% dan 82,48%. Resistance of 13 Cashew Germplasm Accessions to Fusarium Root Rot Disease ABSTRACT Root rot caused by Fusarium is an important cashew disease which attacks any stage of cashew growing from seedlings to adult plant. Therefore, findings of new cashew variety being resistant to the disease should be done in breeding program. The aim of this work was to assess resistance of cashew accession numbers to the disease. Cashew seedlings derived from open pollinated of 13 cashew accessions were observed at germplasm collection of the crop planted at Cikampek Research Station. This work was held in nursery with 50% light intensity of paranet at Pakuwon Research Station. Treatments were arranged in randomized complete block design with three replications. Artificial innoculation used Fusarium isolated from soil was diluted in sterilized water with density of 108 conidia/ml was innoculated to the seedlings. Result showed that allf cashew accessions tested were sucecptable to the disease attack. Lembor 2, M Z Lux, and small Ekoae accessions revealed high in disease incidence which reached 93.33%. Whereas, JN 26 showed the lowest disease incidence, i.e. only 63.33%. The most severe disease symptom was show by Menini 15 (83.56%), although it was not significantly different from Kodi 2 and Kobawani reaching of 82.92% and 82.48%, respectively.

Evaluasi Tingkat Serangan Penggerek Buah Kopi Hypothenemus hampei (Coleoptera: Curculionidae) pada Kultivar Kopi Arabika AGK-1 Funny Soesanthy; Enny Randriani; Syafaruddin Syafaruddin
Jurnal Tanaman Industri dan Penyegar Vol 3, No 3 (2016): Jurnal Tanaman Industri dan Penyegar
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jtidp.v3n3.2016.p167-174

Kopi Arabika berbuah kuning (AGK-1) merupakan kultivar unggul lokal yang telah dikembangkan secara luas di wilayah Kabupaten Garut dan beberapa kabupaten lainnya di Jawa Barat. Keunggulan kultivar ini adalah produktivitas tinggi, ukuran biji besar, dan mutu citarasa spesialti. Salah satu kendala dalam budi daya tanaman kopi adalah serangan kumbang penggerek buah kopi (PBKo) Hypothenemus hampei, yang merupakan hama penting tanaman kopi di seluruh dunia. Tujuan penelitian adalah mengevaluasi tingkat serangan hama PBKo pada kultivar AGK-1 di lapangan dan laboratorium. Penelitian dilakukan mulai bulan Maret sampai Agustus 2016. Pengamatan tingkat serangan PBKo di lapangan dilakukan di blok Ciawer dan Legok Gede, Desa Margamulya, Kecamatan Cikajang, Kabupaten Garut, Jawa Barat pada tanaman berumur 4 tahun (1.450 m dpl), 6 tahun (1.300 m dpl), 9 tahun (1.350 m dpl), dan 11 tahun (1.300 m dpl). Pengujian tingkat serangan PBKo terhadap buah kopi AGK-1 dilakukan di Laboratorium Proteksi Tanaman Balai Penelitian Tanaman Industri dan Penyegar (Balittri), Sukabumi, menggunakan metode pilihan dan tanpa pilihan. Hasil penelitian menunjukkan bahwa persentase serangan H. hampei pada kultivar AGK-1 di lapangan pada semua umur tanaman dan elevasi sangat rendah (3,24%–6,76%). Hasil pengujian di laboratorium menunjukkan persentase serangan PBKo pada kultivar AGK-1 cukup tinggi, baik menggunakan metode tanpa pilihan maupun pilihan, yaitu masing-masing 51,25% dan 17,5%. Akan tetapi, tingkat kerusakan biji yang ditimbulkan tergolong rendah dan tidak berbeda nyata dengan kultivar/varietas lainnya, yaitu 19,37% dan 6,25%.

Pemanfaatan Teknik Random Amplified Polymorphic DNA ( RAPD ) Untuk Pengelompokan Secara Genetik Plasma Nutfah Jambu Mete (Annacardium occidentale L.) Enny Randriani; Cici Tresniawati; Syafaruddin Syafaruddin
Jurnal Tanaman Industri dan Penyegar Vol 3, No 1 (2012): Buletin Riset Tanaman Rempah Dan Aneka Tanaman Industri
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jtidp.v3n1.2012.p1-6

Budidaya jambu mete di Indonesia selama ini belum menggunakan varietas unggul sehingga mengakibatkan rendahnya produksi, yaitu sekitar 493 kg/ha/tahun. Peningkatan genetik terkendala oleh kurangnya informasi tentang variabilitas genetik jambu mete. Dalam merakit suatu varietas unggul diperlukan variabilitas genetik yang luas dari plasma nutfah yang tersedia. Tujuan dari penelitian ini adalah mengetahui tingkat kekerabatan dan keragaman genetik koleksi plasma nutfah jambu mete berdasarkan profil pita DNA menggunakan teknik RAPD. Penelitian dilaksanakan di Laboratorium Biologi Molekuler BB-Biogen, Bogor mulai bulan Mei-November 2010. Materi genetik yang digunakan adalah JN26, Oniki1, Oniki2, Oniki3, Kodi4, NDR31, Nigeria P9, Nigeria P2, JN7, Srilanka, Mojokerto, Pamotan, Karimun, Larantuka, BO2, SM9, JT21 dengan menggunakan 25 primer. Adapun kegiatannya meliputi pengumpulan materi koleksi plasma nutfah jambu mete (17 aksesi). Dilanjutkan kegiatan di Laboratorium dengan tahapan-tahapan kegiatan, seperti ekstraksi dan purifikasi DNA, loading dan running produk PCR dan analisis RAPD serta analisis data. Hasil penelitian menunjukkan dari dua puluh lima primer PCR-RAPD yang digunakan untuk mengamplifikasi sebanyak 17 sampel jambu mete, terdapat 24 primer yang memberikan pita DNA, 21 di antaranya polimorfisme dan tiga primer menunjukkan monomorfis. Hasil analisis kekerabatan 17 sampel jambu mete dengan program NTSys 2.1 menunjukkan bahwa terdapat variasi genetik yang cukup tinggi. Pada koefisien 88%, 17 jambu mete tersebut mengelompok menjadi lima, kelompok yang pertama terdiri dari delapan individu (Oniki1, Kodi4, JN26, NDR31, Srilanka, Mojokerto, Karimun, dan JT21), kelompok dua terdiri dari lima individu (Nigeria P9, B02, Nigeria P2, JN7, Pamotan), kelompok tiga terdiri dua individu (SM9, dan Larantuka), kelompok empat terdiri dari satu individu (Oniki3), dan kelompok lima terdiri dari satu individu (Oniki2). Use of Random Amplified Polymorphic DNA (RAPD) Technique on grouping cashew (Anacardium occidentale L.) germplasmABSTRACT Many cashew plantations in Indonesia do not use superior variety. As a result, national cashew production is only 493 kg/ha/year. Genetic improvement is limited by the lack of information of genetic variability of germplasm. Wide genetic variability in cashew germplasms is necessary to produce superior variety. The purpose of this study was to evaluate the genetic variation and relationship of among cashew germplasms based on band pattern of DNA by using RAPD technique. The experiment was conducted at Molecular Biology Laboratory of BB-Biogen, Bogor since May till November 2009. Genetic materials used were JN26, Oniki1, Oniki2, Oniki3, Kodi4, NDR31, Nigeria P9, Nigeria P2, JN7, Srilanka, Mojokerto, Pamotan, Karimun, Larantuka, BO2, SM9, and JT21 by using 25 primers. The activity consisted of collecting of cashew germplasm (17 accessions), followed with laboratory activities such as: DNA extraction and purification, loading and running of PCR product, RAPD and data analysis. Results shows that 25 primers used were 24 primers shown DNA band pattern 21 of which there are polymorphism and 3 the monomorphism. Germplasm collection of cashew has wide variation. At 88% coefficient, 17 accessions of cashew were divided into five clusters. The first cluster consisted of 8 individuals (Oniki 1, Kodi4, JN26, NDR31, Srilanka, Mojokerto, Karimun, dan JT 21), the second cluster of five individuals (Nigeria P9, B02, Nigeria P2, JN7, Pamotan ), third cluster two individuals (SM9 and Larantuka), the fourth cluster of one individual (Oniki3) and the fifth cluster consisted of one individual (Oniki2).

Evaluasi Ukuran Biji Beras, Kadar Kafein, dan Mutu Cita Rasa Lima Kultivar Kopi Arabika Enny Randriani; Dani Dani; Edi Wardiana
Jurnal Tanaman Industri dan Penyegar Vol 1, No 1 (2014): Jurnal Tanaman Industri dan Penyegar
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jtidp.v1n1.2014.p49-56

Perbaikan mutu fisik, biokimia, dan cita rasa berbasis kultivar penting dilakukan saat ini untuk meningkatkan daya saing kopi Indonesia di pasar global. Penelitian ini bertujuan mengevaluasi keragaman ukuran biji, kadar kafein, dan mutu cita rasa lima kultivar kopi Arabika, yaitu ABP-1, ABP-2, ABP-3, AGK-1, dan S-795. Kelima kultivar tersebut ditanam pada tahun 2008 oleh petani di Desa Marga Mulya, Kecamatan Cikandang, Kabupaten Garut, Jawa Barat pada ketinggian 1.300 m di atas permukaan laut. Biji dari masing-masing kultivar dipanen pada bulan Juli-Agustus 2013 melalui prosedur pengolahan basah. Sampel sebanyak 100 biji beras dari masing-masing kultivar diambil secara acak untuk pengukuran panjang, lebar, tebal, dan bobot 100 biji beras. Pengukuran tersebut diulang sebanyak tiga kali. Analisis varian satu arah dan analisis gerombol dilakukan terhadap data hasil pengukuran. Selain itu, sampel sebanyak 500 gram biji beras dari masing-masing kultivar digunakan untuk pengujian mutu fisik, kimia, dan cita rasa. Ukuran biji beras diklasifikasikan berdasarkan standar SNI 01-2907-2008, sedangkan kandungan kafein diuji berdasarkan prosedur Official Method of Analysis AOAC. Penilaian mutu seduhan mengacu kepada protokol Specialty Coffee Association of America (SCAA). Hasil pengujian menunjukkan bahwa biji beras kultivar ABP-1, ABP-2, AGK-1, dan S-795 termasuk dalam kategori besar, meskipun berdasarkan analisis gerombol terbagi ke dalam dua kelompok. Hanya kultivar ABP-3 yang memiliki ukuran biji beras tergolong kecil dan mengelompok sendiri. Kandungan kafein biji kultivar ABP-1, ABP-2, dan S-795 di bawah 1%, sedangkan ABP-3 dan AGK-1 lebih besar dari 1%. Meskipun demikian, semua kultivar yang diuji termasuk dalam kategori spesialti karena nilai akhirnya mencapai > 80,00.Kata Kunci: Kopi Arabika, spesialti, seleksi, spesifik lokasiCultivar-based quality improvement of Arabica coffee is very important in order to increase competitiveness of Indonesian coffee product in global market. The objectives of this study were to identify the diversity of green bean size, levels of caffeine, and quality among five Arabica coffee cultivars that cultivated by farmers in West Java, ABP-1, ABP-2, ABP-3, AGK-1, and S-795. The research was conducted at 1.300 m above sea level in Garut, West Java, Indonesia. Ripe cherries samples of each cultivars grown in the same area was taken in July-August 2013. Seeds were separated from the rind using wet processing procedure. Sample of 100 green beans were randomly taken for measurement of length, width, thickness, and weight of 100 green beans. Measurements were repeated three times and collected data were analyzed with analysis of variance and analysis of clusters methods. In addition, samples of 500 grams of green beans were taken from each cultivars and subsequently used for testing the quality of the physical, chemical and cupping. Green bean size was determined according to SNI 01-2907-2008, while caffeine content was analysed using AOAC Official Method of Analysis. Cupping test protocol was refer to the Specialty Coffee Association of America (SCAA) method. The results showed that green bean size of ABP-1, ABP-2, AGK-, and S-795 cultivars were classified as large, even though they were clustered into two distinct groups. On the other hand, ABP-3 cultivar produced a small green bean size and solely separated into third group. Caffeine content of ABP-1, ABP-2, and S-795 cultivars were of < 1%, meanwhile ABP-3 and AGK-1 cultivars were of >1%. However, the quality and taste of all cultivars have very good cup quality (score> 80) and meets the criteria for specialty coffee.

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