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Samsudin Samsudin
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Pengaruh Penambahan Gliserol pada Media Perbanyakan terhadap Daya Simpan Biofungisida Trichoderma Widi Amaria; Yulius Ferry; Samsudin Samsudin; Rita Harni
Jurnal Tanaman Industri dan Penyegar Vol 3, No 3 (2016): Jurnal Tanaman Industri dan Penyegar
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jtidp.v3n3.2016.p159-166

Abstract

Daya simpan biofungisida yang mengandung Trichoderma virens dan T. amazonicum untuk mengendalikan penyakit jamur akar putih (JAP) pada tanaman karet penting untuk diketahui agar tetap efektif ketika diaplikasikan. Komposisi media perbanyakan dalam biofungisida Trichoderma sp. dapat mempengaruhi lama hidup dan viabilitas konidia selama penyimpanan. Tujuan penelitian adalah mengetahui pengaruh penambahan gliserol dalam media perbanyakan terhadap daya simpan biofungisida T. virens dan T. amazonicum. Penelitian dilakukan di Laboratorium Proteksi Tanaman, Balai Penelitian Tanaman Industri dan Penyegar (Balittri), Sukabumi, mulai bulan Januari sampai Juli 2014. Percobaan menggunakan rancangan acak lengkap (RAL) dengan 8 perlakuan dan 3 ulangan. Biofungisida yang dibuat terdiri dari: (1) penambahan gliserol (0%, 3%, 6%, dan 9%) pada media perbanyakan T. virens dan (2) penambahan gliserol (0%, 3%, 6%, dan 9%) pada media perbanyakan T. amazonicum. Masing-masing hasil perbanyakan dicampur dengan bahan pembawa talk, dikeringanginkan, selanjutnya dikemas dalam kantong plastik dan disimpan selama 4 bulan. Pengamatan dilakukan setiap bulan, meliputi: jumlah konidia dan populasi Trichoderma sp., serta kadar air biofungisida. Hasil penelitian menunjukkan penambahan gliserol pada media perbanyakan dapat membantu mempertahankan viabilitas T. virens dan T. amazonicum serta daya simpan biofungisida. Penambahan gliserol 6% sampai 9% pada media perbanyakan T. virens dan T. amazonicum merupakan konsentrasi terbaik, menghasilkan konidia 7,98 x 107 – 8,59 x 107 konidia/g dan kelimpahan populasi 11,67 x 103 – 14,67 x 103 cfu/g pada biofungsida yang disimpan selama 4 bulan.
Pengaruh Beberapa Jenis Formula Insektisida Nabati untuk Melindungi Buah Kakao dari Serangan Penggerek Funny Soesanthy; Samsudin Samsudin
Jurnal Tanaman Industri dan Penyegar Vol 1, No 2 (2014): Jurnal Tanaman Industri dan Penyegar
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jtidp.v1n2.2014.p69-78

Abstract

Penggunaan insektisida sintetik yang terus menerus untuk mengendalikan penggerek buah kakao (PBK) dapat merusak keseimbangan ekosistem di perkebunan kakao. Oleh sebab itu, diperlukan cara pengendalian yang relatif aman bagi manusia dan lingkungan, yaitu menggunakan insektisida nabati. Tujuan penelitian adalah menguji keefektifan formula insektisida nabati berbahan dasar ekstrak daun bandotan-metanol, bawang putih-etanol, dan kemiri sunan untuk melindungi buah kakao dari infestasi PBK. Penelitian dilaksanakan mulai bulan Januari-Desember 2013. Bahan uji yang digunakan adalah bandotan-metanol+serai wangi (BMS), bandotan-metanol+minyak cengkeh (BMC), bandotan-metanol+bawang putih-etanol (BMP), bawang putih-etanol+serai wangi (PES), bawang putih-etanol+minyak cengkeh (PEC), kemiri sunan+bawang putih-etanol (KSP), kemiri sunan+bandotan-metanol (KSB), α-eleostearic acid (kontrol negatif), dan air (kontrol positif). Formula dibuat di Laboratorium Proteksi Tanaman, Balai Penelitian Tanaman Industri dan Penyegar (Balittri) Sukabumi, dan pengujiannya dilakukan di perkebunan kakao PT. Bumiloka Swakarya, Sukabumi. Perlakuan disusun dalam unit-unit percobaan yang masing-masing terdiri dari 16 pohon (4 x 4 pohon) dan diulang tiga kali. Pada setiap plot dipilih 30 buah kakao sehat berukuran 6-10 cm. Konsentrasi formula 5% dan 10% dengan volume larutan 250 ml/pohon disemprotkan ke seluruh permukaan buah dan cabang-cabang horizontal, dengan interval 2 minggu sekali sebanyak 6 kali. Pengamatan dilakukan terhadap tingkat serangan PBK dan kerusakan buah yang dipanen. Hasil penelitian menunjukkan bahwa formula KSB (kemiri sunan 25% + bandotan 5%) pada konsentrasi 10 ml/l menghasilkan nilai persentase serangan PBK terendah, sedangkan formula BMP (bandotan 5% + bawang putih 5%) pada konsentrasi 10 ml/l menyebabkan intensitas serangan PBK dan kehilangan hasil terendah.Kata kunci: Insektisida nabati, penggerek buah kakao, formulaThe use of synthetic insecticide continuously to control the cocoa pod borer (CPB) can cause serious damage to the ecosystem balance in the cocoa plantations. Therefore, a control measures that are relatively safe for humans and the environment, such as the use of botanical insecticide are needed. The purpose of the study was to analyze the effectiveness of plant-based insecticide from leaves goat weed-methanol and garlic-ethanol extract, and phillipine tung oil formula to protect cocoa pods from CPB infestation. The study was conducted from January to December 2013. The test materials used were goat weed-methanol+citronella (BMS), goat weed-methanol+clove oil (BMC), goat weed-methanol+garlic-ethanol (BMP), garlic-ethanol+citronella (PES), garlic-ethanol+clove oil (PEC), phillipine tung oil+garlic-ethanol (KSP), phillipine tung oil+goat weed-methanol (KSB), α-eleostearic acid (negative control), and water (positive control). All of the formulas were made in the Plant Protection Laboratory, Indonesian Industrial and Beverages Crops Research Institute (IIBCRI) Sukabumi, whereas the field testing was conducted in cocoa plantations of PT Bumiloka Swakarya, Sukabumi. Each experimental plot consisted of a 4x4 trees, repeated 3 times. In each plot selected 30 healthy cocoa pods measuring 6-10 cm in length. Distance between plots was 2 arrays of trees. Formulas concentration were 5% and 10%, which then sprayed onto the entire surface of the pods and horizontal branches using a knapsack sprayer, 6 times at intervals of 2 weeks. Solution volume was 250 ml / tree. Observations were made on the level of CPB infestation and pod damage harvested. The results showed that the lowest percentage of CPB infestation was on KSB 10 (philippine tung oil 25% + goat weed 5%), whereas the lowest percentage of intensity and yields loss were on BMP 10 (goat weed 5% + garlic 5%).
Potensi Lecanicillium lecanii untuk Pengendalian Helopeltis antonii pada Tanaman Teh Gusti Indriati; Samsudin Samsudin; Widi Amaria
Jurnal Tanaman Industri dan Penyegar Vol 2, No 2 (2015): Jurnal Tanaman Industri dan Penyegar
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jtidp.v2n2.2015.p99-106

Abstract

Lecanicillium lecanii is an entomopathogenic fungus that potential to control tea mosquito bug Helopeltis antonii. The research aimed to determine the potential of L. lecanii in controlling H. antonii in tea plant. The research was conducted at the Laboratory of Plant Protection, Indonesian Industrial and Beverages Crops Research Institute (IIBCRI) Sukabumi, from January to October 2014. The study consisted of infectivity and antifeedancy of L. lecanii on H. antonii nymph, as well as its in vitro compatibility with botanical insecticide, using completely randomized design (CRD). The infectivity and antifeedancy tests of L. lecanii on H. antonii nymph were carried out using 4 densities: 106, 107, 108, 109 conidia/ml, and control. Each treatment of infectivity test was repeated 4 times with observed variables were mortality and development of nymphs. Meanwhile, antifeedancy test was repeated 10 times with the observed variable was percentage of feeding reduction. The in vitro compatibility test of L. lecanii was done by using Piper retrofractum (PR), Tephrosia vogelii (TV) and Annona squamosa (AS) powder with the concentration of 2.5%, 5.0%, 7.5%, respectively and control, which repeated 4 times. The results showed that L. lecanii capable to infect and kill H. antonii nymph at about 65% in laboratory and reduce feeding about 83.04%. Addition of A. squamosa and T. vogelii powder at 2.5%–7.5% concentration were not significantly affecting the growth of L. lecanii colonies on PDA media. On the other hand, addition of P. retrofractum powder with the same concentration was considerably inhibiting the growth of L. lecanii. This result demonstrates that A. squamosa and T. vogelii powder are compatible with L. lecanii. 
Pengaruh Metode Pengolahan dan Suhu Penyangraian terhadap Karakter Fisiko-Kimia Kopi Robusta Nanda Supriana; Usman Ahmad; Samsudin Samsudin; Eko Heri Purwanto
Jurnal Tanaman Industri dan Penyegar Vol 7, No 2 (2020): Jurnal Tanaman Industri dan Penyegar
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jtidp.v7n2.2020.p61-72

Abstract

The processing of coffee can be classified into 3 types namely: dry, semi-wet and wet processing. And then, roasting is the process of forming the taste and aroma of coffee. The study was conducted at Pakuwon Experimental Station, Coffee and Cacao Processing Units, and Integrated Laboratory of Balittri, and TTPHP Laboratory of IPB Bogor, from July until October 2019. The study aimed to investigate (1) the effect of processing methods on physical quality characters of green beans, and (2) the effect of processing methods and roasting temperatures on chemical characters of coffee roasted and organoleptic characters of coffee brewed. The completely randomized design in factorial 2 factors and 3 replications was used in this study. The first factor were 3 type of processing methods (dry, semi-wet, and wet), and the second factor were 2 level of roasting temperatures (180oC and 210oC). The variables observed in the green beans were physical quality characters, while in roasted coffee were water content, caffeine content, and antioxidant activity, and in brewed coffee were organoleptic characters. Result showed that the best green beans was produced from a wet processing method with a minimum total defect value of 21.33 (quality 2). Roasting temperature has a very significant effect (p<0.01) on water content and antioxidant activity, while caffeine content is not significantly affected. Based on the results of cupping test, the best brewed coffee (final score >80.00) was obtained from the treatments of a dry, semi-wet and wet processing method with a roasting temperature of 210°C.
INFEKTIFITAS Spodoptera exigua NUCLEOPOLYHEDROVIRUS (SeNPV) YANG DIPERKAYA DENGAN BAHAN PENGAKTIF TERHADAP LARVA Spodoptera exigua Huebner Samsudin Samsudin; Teguh Santoso
BERITA BIOLOGI Vol 11, No 3 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v11i3.499

Abstract

Spodoptera exigua Huebner nucleopolyhedrovirus (SeNPV) is an entomophathogenic virus of onion caterpillar S. exigua larvae commonly used as bioinsecticide. The major limitations of SeNPV for biocontrol of onion caterpillar is it requires long time for the virus to kill the insect host. This research was aimed to find out the material as phagostimulant to increase the infectivity of SeNPV and to determine the optimum boric acid concentration as an enhancer for SeNPV activities. This research was conducted at laboratory using Cipanas isolate of SeNPV and third instar of S. exigua larvae. Phagostimulant used are 5% ofsucrose, soybean sauces, molases, sugar and turmeric filtrate,and the enhancers used are 0,1%, 1%, 5% and 10% concentration of boric acid. This result indicated that soybean sauces and sucrose increased S. exigua consumption and enhanced the infectivity of SeNPV. When mixed with polyhedra of SeNPV, 1% to 5% sucrose significantly increased S. exigua consumption and increased the infectivity of SeNPV, while 10% sucrose tended to decrease the feeding of S. exigua. Boric acid enhanced the infectivity of SeNPV. However, the increase of boric acid concentration should be restricted maximum at 5% for avoiding the negative impacts on the environment.
Co-Authors Abdul Muis Hasibuan Bedy Sudjarmoko Dewi Listyati Efi Taufik Eko Heri Purwanto Funny Soesanthy Gusti Indriati Nanda Supriana Rita Harni Rita Harni Rubiyo Rubiyo Syafaruddin Syafaruddin Teguh Santoso Usman Ahmad Widi Amaria YULIUS FERRY