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M. Azrai
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MOLECULAR CHARACTERIZATION OF QUALITY PROTEIN MAIZE AND DOWNY MILDEW RESISTANCE LINES BASED ON SIMPLE SEQUENCE REPEATS (SSRs) MARKER D. Ruswandi; N. Wicaksana; M. B. Pabendon; M. Azrai; M. Rachmadi; A. Ismail; N. Carsono; F. Damayanti; F. Kasim
Zuriat Vol 16, No 1 (2005)
Publisher : Breeding Science Society of Indonesia (BSSI) / PERIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/zuriat.v16i1.6779

Abstract

The information on germplasm diversity and genetic relatedness among elite breeding materials is an important element in maize breeding. Molecular characterization and genetic relationship of 11 QPM-DMR lines were analysed using thirty three SSRs markers. Genetic relationship was determined using Jaccard’s similarity coefficient, and dendogram was then constructed based on the unweighted pair-group method with arithmetical averages (UPGMA). Result showed that (i) all SSRs loci were informative for describing the genotypic variation as showed by their PIC, which ranged from 0.19 for umc1304 to 0.93 for phi112; (ii) the eleven maize inbred lines were clustered into one major group A and small groups B and C that corresponds well with the breeding programs adopted at different institutes of release, and (iii) thus, SSRs marker system is a valuable marker for varietals identification and for genetic diversity study of elite breeding materials.
Pemetaan QTL Resistensi Tanaman Jagung terhadap Penyakit Bulai Di Bogor M. Azrai; Murdaningsih H. K.; Neni Rostini; Sugiono Moeljopawiro; D. Ruswandi
Zuriat Vol 13, No 2 (2002)
Publisher : Breeding Science Society of Indonesia (BSSI) / PERIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/zuriat.v13i2.6747

Abstract

Sebagai bagian dari suatu penelitian kolaboratif AMBIONET (Asian Maize Biotechnology Network), Indonesia turut serta dalam kegiatan marker assisted selection (MAS) untuk pemuliaan jagung tahan bulai. Tujuan penelitian adalah untuk mengidentifikasi dan memetakan 146 marka RFLP (Restriction Fragment Length Polymorphism) yang berasosiasi dengan lokus karakter kuantitatif ketahanan dari 134 populasi genotip RIL (recombinant inbred lines) progeni CML 139 x Ki3. Penelitian dibagi atas dua tahap yaitu tahap analisis molekuler dan tahap pengujian di lapangan. Analisis molekuler memanfaatkan data dari Pusat Penelitian Internasional Jagung dan Terigu (CIMMYT), Mexico. Penelitian lapangan dilakukan di Bogor pada bulan Mei 2002 sampai Juli 2002. Data fenotipik (persentase serangan bulai) dianalisis dengan menggunakan program alpha lattice, sedangkan analisis QTL (Quantitative Trait Loci) menggunakan “software computer” program “Windows QTL Cartographer V.1.21”. Hasil analisis QTL menunjukkan bahwa dari 146 marka RFLP pada 134 populasi genotip RIL progeni CML 139 x Ki3, terdapat 14 marka yang terdistribusi pada enam kromosom dari 10 kromosom berdasarkan fenotaiping di Bogor. Efek aditif yang terdeteksi dalam analisis QTL pada umumnya bernilai negatif yang mengarah ke CML 139 (genotip peka).
Co-Authors A. Ismail D. Ruswandi F. Damayanti F. Kasim M. B. Pabendon M. Rachmadi Murdaningsih H. K. N. Carsono N. Wicaksana Neni Rostini SUGIONO MOELJOPAWIRO