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HANIFAH YUSUF
Bagian Farmakologi Fakultas Kedokteran Universitas Syiah Kuala
Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Energy Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Physics Public Health Veterinary

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Journal : Jurnal Natural

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Anticancer activity and apoptotic induction of Chromolaena odorata Linn leaves extract and fractions on hepatocellular carcinoma cell lines (HepG2) HANIFAH YUSUF; MARHAMI FAHRIANI
Jurnal Natural Volume 22 Number 1, February 2022
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1122.825 KB) | DOI: 10.24815/jn.v22i1.22854

Abstract

The purpose of this study is to screen and to evaluate anticancer activity and apoptosis induction of Chromolaena odorata (C. odorata) leaves extract and its fractions on HepG2 cancer cell lines. The C. odorata leaves were extracted by maceration using ethanol 80% then fractionated by using n-hexane, ethyl acetate and ethanol. MTT assay method was used to evaluate anticancer activity and flowcytometri method used to evaluate the induction of apoptosis. The phytochemical analysis and  characterization of the major compound of the extract and the fractions were done by using Liquid Chromatography-Mass Spectroscopy (LCMS). The phytochemical analyis showed that the crude ethanolic extract and fractions of C. odorata leaves contains alkaloid, flavonoids, phenolic, steroids, tannin and saponin. The result of LCMS shown the major compounds of the extract and fractions are 5,7,8,3′,4′-Pentamethoxyflavonone, 1-Carboethoxy-β-carboline, 3-Methylcanthin-2, 6- dion, Canthin-6-one. In the n-hexane fraction obtained 5,7,8,3′,4′-Pentamethoxyflavonone, Methyl ophiopogonanone A and Pd-C-Ⅱ. Meanwhile, in the ethyl acetate fraction contained, 3-(3′,4′-Dihydroxybenzyl)-7-hydroxychroman-4-one, 3-(4'-Hydroxy-benzyl)-5,7-dihydroxy-6-methyl-8- methoxy-chroman-4-one, Methyl opiopogonanone A and Sinensetin. The ethanol fraction, contained the compound 3-(3′,4′-Dihydroxybenzyl)-7-hydroxychroman-4-one, 3-(4'-Hydroxy-benzyl)-5,7-dihydroxy-6-methyl-8- methoxy-chroman-4-one and Methyl opiopogonanone A. The crude ethanolic extract, n-hexane fraction, ethyl acetate and ethanol fraction extract have anticancer activities with IC50 value of 23.44 mg/mL, 84.52mg/mL; 88.51mg/mL; and 167.49 mg/mL respectively. This research suggests the crude ethanolic extract of Chromolaena odorata leaves has a potential candidate for hepatocarcinoma treatment.
Anticancer activity and apoptotic induction of Chromolaena odorata Linn leaves extract and fractions on hepatocellular carcinoma cell lines (HepG2) HANIFAH YUSUF; MARHAMI FAHRIANI
Jurnal Natural Volume 22 Number 1, February 2022
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jn.v22i1.22854

Abstract

The purpose of this study is to screen and to evaluate anticancer activity and apoptosis induction of Chromolaena odorata (C. odorata) leaves extract and its fractions on HepG2 cancer cell lines. The C. odorata leaves were extracted by maceration using ethanol 80% then fractionated by using n-hexane, ethyl acetate and ethanol. MTT assay method was used to evaluate anticancer activity and flowcytometri method used to evaluate the induction of apoptosis. The phytochemical analysis and  characterization of the major compound of the extract and the fractions were done by using Liquid Chromatography-Mass Spectroscopy (LCMS). The phytochemical analyis showed that the crude ethanolic extract and fractions of C. odorata leaves contains alkaloid, flavonoids, phenolic, steroids, tannin and saponin. The result of LCMS shown the major compounds of the extract and fractions are 5,7,8,3′,4′-Pentamethoxyflavonone, 1-Carboethoxy-β-carboline, 3-Methylcanthin-2, 6- dion, Canthin-6-one. In the n-hexane fraction obtained 5,7,8,3′,4′-Pentamethoxyflavonone, Methyl ophiopogonanone A and Pd-C-Ⅱ. Meanwhile, in the ethyl acetate fraction contained, 3-(3′,4′-Dihydroxybenzyl)-7-hydroxychroman-4-one, 3-(4'-Hydroxy-benzyl)-5,7-dihydroxy-6-methyl-8- methoxy-chroman-4-one, Methyl opiopogonanone A and Sinensetin. The ethanol fraction, contained the compound 3-(3′,4′-Dihydroxybenzyl)-7-hydroxychroman-4-one, 3-(4'-Hydroxy-benzyl)-5,7-dihydroxy-6-methyl-8- methoxy-chroman-4-one and Methyl opiopogonanone A. The crude ethanolic extract, n-hexane fraction, ethyl acetate and ethanol fraction extract have anticancer activities with IC50 value of 23.44 mg/mL, 84.52mg/mL; 88.51mg/mL; and 167.49 mg/mL respectively. This research suggests the crude ethanolic extract of Chromolaena odorata leaves has a potential candidate for hepatocarcinoma treatment.
Co-Authors Andri Frediansyah Cut Murzalina Endang Mutiawati Rahayuningsih* Firzan Nainu Firzan Nainu Francesco Rosiello Helnida A. Maliga Jhonny K. Fajar Jonny K. Fajar Kurnia Fitri Jamil Marhami Fahriani Marhami Fahriani MARHAMI FAHRIANI Milda Husnah Muhammad Ilmawan Muhammad Ilmawan Reno Keumalazia Kamarlis Salin Sirinam Sandro GV. Rosa Sri Masyeni, Dewa Ayu Putri Sukamto Mamada Synat Keam Tristia Rinanda Youdiil Ophinni Youdiil Ophinni Yusni Yusni Yusni Yusni