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All Journal Indonesian Journal of Pharmaceutical and Clinical Research Journal Of Carbazon
Hira Helwati
Chemistry Department, Faculty of Mathematics and Natural Sciences, Syiah Kuala University
Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health

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Uncovering the Endogenous Bacteria Involved in the Synthesis of Formaldehyde in Tiger Grouper Fish (Epinephelus fuscoguttaus) Sufriadi, Elly; Helwati, Hira; Nurfahmi, Nurfahmi
Journal of Carbazon Vol 1, No 1 (2023): June 2023
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jokarbazon.v1i1.32844

Abstract

This study aimed to isolate and identify specific bacteria responsible for the conversion of trimethylamine oxide (TMAO) into formaldehyde in tiger grouper (Epinephelus fuscoguttatus). The gastrointestinal tract of tiger grouper specimens was sampled, and bacterial colonies were isolated using nutrient agar (NA) and screened on TMAO media. Bacterial isolates capable of producing formaldehyde were identified. The implications of these findings extend to food safety, scientific research, medical diagnostics, and industrial applications. Further investigation is needed to elucidate the enzymatic pathways and genetic factors associated with formaldehyde production in these bacteria. Evaluation of the antibacterial compound production capability revealed inhibitory zones against Escherichia coli, indicating the potential for bactericidal properties. Gram staining and scanning electron microscopy (SEM) were employed to characterize the formaldehyde-producing bacteria, revealing their gram-positive nature and rod-shaped morphology. This study provides insights into the role of specific bacteria in formaldehyde production, paving the way for future research and applications in various fields.
Antibacterial Activities of Perfume: Combination Flower Magnolia alba, Cananga odorata and Mimusops elengi L, Fixed with Pogostemon cablin Oil Nasution, Rosnani; Azwar, Azalia Izdhihar; Helwati, Hira; Marianne
Indonesian Journal of Pharmaceutical and Clinical Research Vol. 2 No. 1 (2019): Indonesian Journal of Pharmaceutical and Clinical Research
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (787.644 KB) | DOI: 10.32734/idjpcr.v2i1.814

Abstract

Abstract: The making of perfume from the combination Magnolia alba (M. alba) flower oil, Cananga odorata (C. odorata) and Mimusops elengi L (M. elengi) fixed with Pogostemon cablin oil (P. cablin) have been done. Essential oil of M. alba and C. odorata was distilled by water distillation and extraction essential oil of M. elengi flower using n-hexane evaporated solvent method. Composition perfume through organoleptic test obtained with a ratio of 4% v/v, 4% v/v, and 2% v/v for each essential oil M. alba, C. odorata and M. elengi. Antibacterial activity of the perfume and each of the volatile oil was performed by the disc diffusion method. Antibacterial activity test showed that perfumes were able to inhibit the growth of Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) bacteria, but less active. Antibacterial activity of the perfume was more active against E. coli with an inhibitory zone of 8.3 mm and 39.81% inhibitory power than S. aureus bacterial 7.3 mm inhibition zone with 37.55% inhibitory power. Perfumes after storage for one, and two months had better antibacterial activity than before storage with an average inhibitory zone of 11-15 mm and inhibitory power of 50- 68,18%. Keywords: Antibacterial, E. Coli, C. Odorata, M. alba, M. elengi, P. cablin, , S. aureus
Co-Authors . Marianne Azwar, Azalia Izdhihar Nurfahmi, Nurfahmi Rosnani Nasution Sufriadi, Elly