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Lies Parede
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The isolation of Gurnbiro virus from larvae and darkling Ivelles (Carcinops pumilin) Parede, Lies; Indriani, Risa; ., Sukarsih
Indonesian Journal of Animal and Veterinary Sciences Vol 2, No 1 (1996)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (472.359 KB) | DOI: 10.14334/jitv.v2i1.42

Abstract

Gumboro (infectious bursal disease, IBD) virus was isolated from darkling beetles (Carrinaps pumilin) and their larvae in a commercial pulletchicken farm with repeated outbreaks incidence of Gumboro disease in Tangertng, West Java. In addition, these over populated beetles and their larvae were suspected to be infected and then shed the virus or acted as vectors. Isolation was done by repeated passages of virus using chicken embryo fibroblast cells as prime media, which then showed the evidence of cylop: ihic effecis. The isolation was followed by antigen detection by means of ELISA test.   Key words: Gumboro disease, infectious bursal disease, darkling beetle, Carcinops punulin  
The macroscopic and microscopic patology changes on broiler infected with local reovirus isolate Wahyuwardani, Sutiastuti; Huminto, Hernomoadi; Parede, Lies
Indonesian Journal of Animal and Veterinary Sciences Vol 10, No 1 (2005)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (337.56 KB) | DOI: 10.14334/jitv.v10i1.478

Abstract

Local reovirus isolate is the virus which could be isolated from runting and stunting syndrome. The ability of local reovirus isolate to induce runting and stunting syndrome in broilers chicken was investigated. The day old chicks (doc) were infected with local reovirus isolate assessed clinically and pathologically at 1, 2 and 3 weeks post inoculation. A total of 40 DOC were divided into two groups. The first group (20 doc) was orally infected with ± 2 x 103 local reovirus isolate particle and the other used as negative control group. The results showed that the isolate caused wet droppings, stunting, enteritis, pancreatitis, malabsorbtion, bursal atrophy and spleenic hypertrophy, which similar to runting and stunting syndrome (RSS) disease in chicken. The body weight was reduced to 14.7% on the inoculated group at 4 weeks post inoculation.     Key Words: Broiler, Local Reovirus Isolate, Runting and Stunting, Malabsorption
The macroscopic and microscopic patology changes on broiler infected with local reovirus isolate Sutiastuti Wahyuwardani; Hernomoadi Huminto; Lies Parede
Jurnal Ilmu Ternak dan Veteriner Vol 10, No 1 (2005): MARCH 2005
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (337.56 KB) | DOI: 10.14334/jitv.v10i1.478

Abstract

Local reovirus isolate is the virus which could be isolated from runting and stunting syndrome. The ability of local reovirus isolate to induce runting and stunting syndrome in broilers chicken was investigated. The day old chicks (doc) were infected with local reovirus isolate assessed clinically and pathologically at 1, 2 and 3 weeks post inoculation. A total of 40 DOC were divided into two groups. The first group (20 doc) was orally infected with ± 2 x 103 local reovirus isolate particle and the other used as negative control group. The results showed that the isolate caused wet droppings, stunting, enteritis, pancreatitis, malabsorbtion, bursal atrophy and spleenic hypertrophy, which similar to runting and stunting syndrome (RSS) disease in chicken. The body weight was reduced to 14.7% on the inoculated group at 4 weeks post inoculation.     Key Words: Broiler, Local Reovirus Isolate, Runting and Stunting, Malabsorption
The isolation of Gurnbiro virus from larvae and darkling Ivelles (Carcinops pumilin) Lies Parede; Risa Indriani; Sukarsih .
Jurnal Ilmu Ternak dan Veteriner Vol 2, No 1 (1996)
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (472.359 KB) | DOI: 10.14334/jitv.v2i1.42

Abstract

Gumboro (infectious bursal disease, IBD) virus was isolated from darkling beetles (Carrinaps pumilin) and their larvae in a commercial pulletchicken farm with repeated outbreaks incidence of Gumboro disease in Tangertng, West Java. In addition, these over populated beetles and their larvae were suspected to be infected and then shed the virus or acted as vectors. Isolation was done by repeated passages of virus using chicken embryo fibroblast cells as prime media, which then showed the evidence of cylop: ihic effecis. The isolation was followed by antigen detection by means of ELISA test.   Key words: Gumboro disease, infectious bursal disease, darkling beetle, Carcinops punulin  
Co-Authors Dewi Ratih Agungpriyono Hernomoadi Huminto I Gusti Ngurah Kade Mahardika Risa Indriani RISA INDRIANI Sukarsih . Sutiastuti Wahyuwardani Sutiastuti Wahyuwardani Wasmen Manalu