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Utilization of Probiotic and Organic-Cn on Ruminal Ecosystem In Vitro Wulansih Dwi Astuti; Roni Ridwan; Baharuddin Tappa
Jurnal Ilmu Ternak dan Veteriner Vol 12, No 4 (2007): DECEMBER 2007
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (52.992 KB) | DOI: 10.14334/jitv.v12i4.493

Abstract

Improvement of nutrient intake was needed to increase cattle productivity. Probiotics and organic Cr, as feed additives, might have beneficially improve ruminant microbial ecosystem, and optimize digestion process by rumen microbes. The objective of this study was to examine the effects of combination between probiotic or mixed probiotcs and organic Cr on rumen fermentation activity. An in vitro study was held based on randomized block design with 5 treatments and 3 replications. The treatments were (A) Control ration, (B) Control ration + probiotic TSD 10, (C) Control ration + probiotic TSD 10 + organic Cr, (D) Control ration + mixed probiotics, and (E) Control ration + mixed probiotics + organic Cr. Organic Cr concentration was supplemented 2 ppm, whereas probiotics supplement contained 109 cfu/g. Supplementation of probiotic and organic Cr increased dry matter and organic matter digestibility but decreased total VFA and NH3 concentration. Treatment E (combination between mixed probiotics and organic Cr) gave the highest dry matter (52.45 %) and organic matter (51.96 %) digestibilities. It also tended to increase NH3 and total VFA production. Supplementation of single or mixed probiotics showed no difference for dry matter and organic matter digestibilities, and proportion of individual VFA. Mixed probiotics gave higher VFA and NH3 concentration compared to single probiotic. Probiotics supplementation resulted in a tendency for higher acetate proportion, while organic Cr supplementation resulted in a tendency of higher propionate proportion. Key Words: Probiotic, Organic Cr, Ruminal Microbes, VFA
In Vitro Ruminal Fermentation and Degradability of Rice Husk on Rice Bran Substitution Rusli Fidriyanto; Roni Ridwan; Wulansih Dwi Astuti; Rohmatussolihat Rohmatussolihat; Nurul Fitri Sari; Muh Watman; Yantyati Widyastuti
ANNALES BOGORIENSES Vol 24, No 1 (2020): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ann.bogor.2020.v24.n1.50-58

Abstract

Rice bran is a by-product of the rice milling process and has been well used as livestock feed. Rice bran is often adulterated with rice husk. The objective of this study was to evaluate the in vitro ruminal fermentation characteristics of rice bran with various compositions of rice husk and assess the relationship between rice husk addition and rice bran quality. The experiment was arranged in a completely randomized design with rice husk addition as a factor and three replications. Data of proximate value, gas production, ruminal degradability, and volatile fatty acid production were analyzed by analysis of variance. Moreover, significant effects of each treatment in the in vitro fermentation were further analyzed by Duncan's multiple range test (P<0.05). It was shown that the addition of rice husk to rice bran could increase acetic acid level, but it reduced potential gas production, gas production rate, organic matter and dry matter digestibility, and propionic acid level. Interestingly, the linear regression of dry matter digestibility, organic matter digestibility, and potential gas production showed the high adjusted R2 values. Moreover, this study also revealed that 10% of rice husk substitution on rice bran could significantly reduce the dry matter digestibility.
PENGARUH SUBSTITUSI RUMPUT GAJAH DENGAN LIMBAH SERAI PADA PAKAN RUMINANSIA TINGGI HIJAUAN TERHADAPKECERNAAN DAN PRODUKSI METAN SECARA IN VITRO Rusli Fidriyanto; Gunawan Priadi; Yashanti Berlinda Paradisa; Wulansih Dwi Astuti; Roni Ridwan; Rohmatussolihat Rohmatussolihat; Ki Ageng Sarwono; Muh Whatman; Yantyati Widyastuti
Agric Vol. 33 No. 2 (2021)
Publisher : Fakultas Pertanian dan Bisnis, Universitas Kristen Satya Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24246/agric.2021.v33.i2.p103-114

Abstract

Serai merupakan bahan baku untuk produksi minyak serai. Indonesia masuk dalam sepuluh negara terbesar produsen minyak serai di dunia. Industri pengolahan minyak serai akan menghasilkan limbah serai. Penelitian ini bertujuan untuk mengetahui pengaruh subtitusi rumput gajah (Pennisetum purpureum) dengan limbah serai terhadap fermentasi rumen secara in vitro. Penelitian ini menggunakan rancangan acak kelompok dengan 5 perlakuan (100R: limbah serai 0%, 75R: limbah serai 25%, 50R: limbah serai 50%, 25R: limbah serai 75%, 0R: limbah serai 100%) dan 4 waktu pengambilan rumen yang berbeda sebagai kelompok. Hasil penelitian menunjukkan bahwa substitusi rumput dengan limbah serai tidak memberikan perbedaan yang signifikan terhadap kadar bahan kering, namun dapat menurunkan kadar protein dan meningkatkan serat kasar serta lemak kasar secara signifikan (P<0,05). Hasil analisis keceranaan in vitro terhadap aktivitas fermentasi rumen, menunjukkan bahwa substitusi serai tidak mempengaruhi pH, kecepatan produksi gas, asam butirat, asam valerat, asam isobutirat, dan asam isovalerat baik dari segi jumlah maupun proporsi. Substitusi rumput dengan limbah serai sebesar 25% (75R) tidak menghasilkan penurunan kadar bahan organik, produksi gas potensial dan produksi metan (jam ke-24) namun terjadi penurunan kadar protein dan meningkatkan lag time secara signifikan (P<0,05). Penggunaan serai sebesar 50% dapat menurunkan produksi gas metan secara signifikan (P<0,05). Peningkatan konsentrasi limbah serai yang digunakan menyebabkan terjadinya perubahan profil VFA parsial yang disebabkan oleh adanya perubahan komposisi asam asetat. Penurunan rasio asam asetat/ propionat (A/P) secara signifikan terjadi pada penggunaan serai sebesar 100%. Penggunaan limbah serai dapat mensubtitusi rumput gajah hingga 25% pada pakan tinggi hijauan tanpa terjadi penurunan pada produksi gas potensial, kecernaan dan total VFA.