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All Journal Jurnal Ilmu Ternak Veteriner WARTAZOA Indonesian Bulletin of Animal and Veterinary Sciences Jurnal Medika Veterinaria
Didik T Subekti
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

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Journal : Jurnal Ilmu Ternak Veteriner

 1 
Effect of methanol extracts of nony fruit on mice infected by RH strain of Toxoplasma gondii Didik T Subekti; Eka S.P Sari; Dwi R Widiastuti; Rica Haerlani; Eka Fitri Diani; Tolibin Iskandar; Dian R Laksmitawati
Jurnal Ilmu Ternak dan Veteriner Vol 10, No 4 (2005): DECEMBER 2005
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (230.196 KB) | DOI: 10.14334/jitv.v10i4.457

Abstract

Intraperitoneal infection of Type I Toxoplasma gondii on mice causes high mortality at a short time due to parasitic burden, immunosuppression, cell and tissue damage. The mice survival is increased after treated with drugs that reduce or destroy tachyzoite and modulate or recovered the immune system. Nony fruit (Morinda citrifolia) is popular as immunomudulator and has antoxoplasma properties. The purpose of this experiment is to evaluate the effect of ethanol extract of nony fruit and Fansidar® (pyrimethamine-sulfadiazine) to reduce tachyzoite and improve survival as well as immunomudulator on mice following toxoplasma infection. Mice was divided into six groups (10 mice respectively) consist of infected-non treated groups, infected + Fansidar®, infected + ethanol extract of nony on several doses (100, 50, 25%) and non infected-non treated groups. All mice on each groups were infected intraperitoneally by 5 x 106 and 2,5 x 103 RH strain of Toxoplasma gondii tachyzoite/mice respectively. The results have shown that Fansidar® was successfully to reduced tachyzoite and improved mice survival but the ethanol extract of nony fruit was failed.     Key Words: Survivality, Immunomodulator, Toxoplasma gondii, Nony extracts
Cloning and Clone Analysis of GRA1 Gene from Local Isolate Toxoplasma gondii Tachyzoite Didik T Subekti; W.T Artama; E Sulistyaningsih; S.H Poerwanto; Y Sari; F Bagaskoro
Jurnal Ilmu Ternak dan Veteriner Vol 13, No 1 (2008): MARCH 2008
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2765.962 KB) | DOI: 10.14334/jitv.v13i1.594

Abstract

The GRA1 gene of Toxoplasma gondii encoding protein called GRA1 protein. GRA1 protein known to be immunogenic and essentialy involved in modification of parasitophorus vacoule which has role in immune evasion and virulency of organism. The local isolate of T. gondii is successfuly isolated and known as highly pathogenic isolate similarly as its RH strain. Unfortunately, the homology sequence of GRA1 gene between those isolate still unknown. The purpose of the research are to clone the GRA1 gene and to analyze the homology from pathogenic T. gondii isolate and RH strain. Tachyzoite of T. gondii was grown in mice peritoneum by intraperitoneal injection. Then, total mRNA was isolated and purified. cDNA was synthesized from mRNA and then amplified using F1 dan R1 primers to get clone of GRA1 from local isolate. Homology analysis was perform using several bioinformatic softwares. The result showed that cDNA of GRA1 from local isolate has 84% homologs with RH strain of T.gondii. However, when subsequently editing performed to parts of suspected non coding sequence of cDNA GRA1 to get CDS of GRA1, the homology was increase to 100% compare to CDS of GRA1 of RH strain. Key words: GRA1, Toxoplasma gondii, Clonning, Expression
Co-Authors Aprilia Wardana Dian R Laksmitawati Dwi R Widiastuti E Sulistyaningsih Eka Fitri Diani Eka S.P Sari F Bagaskoro Febiola Rama Sari Nurfida K Arrasyid Rica Haerlani S.H Poerwanto Tolibin Iskandar Ummu Balqis W.T Artama Y Sari Yudha Fahrimal