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All Journal Jurnal Ternak Tropika
Rafika Febriani Putri
Fakultas Peternakan, Universitas Brawijaya Jalan Veteran, Ketawanggede, Kec. Lowokwaru, Kota Malang, Jawa Timur, Indonesia 65145

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Single Nucleotide Polymorphims (snps) Identification of Inhibin Sub Unit-α (inha) Gene on Madura Bulls Irida Novianti; Chairdin Dwi Nugraha; Rafika Febriani Putri; Ahmad Furqon; Wike Andre Septian; S. Rahayu; V. M. A. Nurgiartiningsih; Suyadi Suyadi
TERNAK TROPIKA Journal of Tropical Animal Production Vol 22, No 1 (2021): TERNAK TROPIKA Journal of Tropical Animal Production
Publisher : Jurusan Produksi Ternak, Fakultas Peternakan, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtapro.2021.022.01.10

Abstract

INHA gene is a gene that is suggested to have role in reproductive system. Single Nucleotide Polymorphisms (SNPs) of Madura Bulls were identified in this study. Polymerase Chain Reactions (PCR) was used to amplify INHA gene region and MEGA 7 program was utilized to align the amplified region sequences with sequence from Ensembl database. Four SNPs found in INHA and they are located at the first exon. Two SNPs were misssense mutations that causing the substitution of amino acid leucine21 by proline, and amino acid valine63 by methionine and the other two SNPs were synonymous mutation. One of the synonymous SNPs was a novel mutation. Based on those identified SNPs, they could be suggested as potential candidate markers of reproduction traits for Madura bulls. Moreover, through heterozygosity value from the observed bulls, it was indicated that the genotype was varied in population. Therefore a molecular selection program could be designed to determine the Madura superior bull.
Identifikasi Keragaman Genetik Gen Inhibin Subunit Alpha (INHA) dan Inhibin Subunit Beta A (INHBA) Pada Sapi Peranakan Ongole (PO) Rafika Febriani Putri; Chairdin Dwi Nugraha; Ahmad Furqon; Wike Andre Septian; Suyadi Suyadi
TERNAK TROPIKA Journal of Tropical Animal Production Vol 22, No 1 (2021): TERNAK TROPIKA Journal of Tropical Animal Production
Publisher : Jurusan Produksi Ternak, Fakultas Peternakan, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtapro.2021.022.01.9

Abstract

Gen Inhibin berperan dalam mengatur proliferasi sel, pertumbuhan kelenjar adrenal, hematopoiesis dan metabolisme tulang. Inhibin subunit alpha juga diketahui memiliki dampak yang signifikan terhadap berat badan, panjang tubuh dan lingkar badan. Penelitian ini bertujuan untuk mengidentifikasi keragaman gen INHA dan INHBA pada Sapi PO yang berturut-turut sebanyak 68 ekor sapi PO yang berasal dari UPT PT HMT Tuban dan 11 ekor sapi PO BBIB Singosari. Metode pendeteksian keragaman gen menggunakan metode sequencing. Hasil penelitian menunjukkan bahwa pada sapi PO populasi UPT Tuban ditemukan tiga genotipe (AA, AB dan BB) pada fragmen gen INHA dan INHBA, sapi PO populasi BBIB Singosari memiliki satu genotipe (AA) pada fragmen gen INHA serta dua genotipe (AA dan BB) pada fragmen gen INHBA. Berdasarkan hasil tersebut dapat disimpulkan bahwa gen INHA dan INHBA pada sapi PO populasi UPT Tuban dalam kondisi beragam (polimorfik). Informasi dalam penelitian ini dapat dijadikan dasar atau landasan dalam memulai program seleksi dan perkawinan sapi potong lokal Indonesia berbasis teknologi molekuler.
Hubungan Antara Umur, Bobot Badan, Lingkar Skrotum Dengan Produksi Spermatozoa Sapi Peranakan Ongole Chairdin Dwi Nugraha; Rafika Febriani Putri; Ahmad Furqon; Wike Andre Septian; Suyadi Suyadi
TERNAK TROPIKA Journal of Tropical Animal Production Vol 22, No 1 (2021): TERNAK TROPIKA Journal of Tropical Animal Production
Publisher : Jurusan Produksi Ternak, Fakultas Peternakan, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtapro.2021.022.01.3

Abstract

Sapi peranakan Ongole atau lebih dikenal dengan sapi PO merupakan salah satu ras sapi lokal yang banyak dipelihara di Indonesia. Pengembangan pada aspek reproduksi dalam pemilihan pejantan unggul menjadi sangat penting. Umur, bobot badan (BB), lingkar skrotum (LS) dan produksi semen merupakan kriteria dalam pemilihan pejantan unggul. penelitian ini bertujuan untuk mengetahui nilai korelasi antara umur, bobot badan dan lingkar skrotum terhadap total spermatozoa (TS) dan total spermatozoa motil (TSM). 11 ekor pejantan sapi PO di UPT PT & HMT Tuban dengan umur 2-8 tahun digunakan dalam penelitian ini. Total 48 ejakulasi dikoleksi selama empat minggu. Metode penelitian adalah observasional. Data dianalisis menggunakan SPSS 24 dengan metode OneWay ANOVA serta hubungan antar variabel menggunakan korelasi pearson (r). Hasil Penelitian menunjukkan bahwa umur berpengaruh nyata (p<0,05) terhadap nilai TS dan TSM. Umur, BB dan LS berkorelasi signifikan (p<0,05) terhadap TS dengan nilai masing masing secara berurutan yaitu (r=0,444), (r=0,615) dan (r=0,404). Umur, BB dan LS berkorelasi signifikan (p<0,05) terhadap TSM dengan nilai (r=0,459), (r=0,635) dan (r=0,402). Pemilihan pejantan unggul sapi PO perlu mempertimbangkan BB, umur dan LS yang tinggi sesuai Standar Nasional Indonesia.
The Effect of Different Breeds and Ages on Semen Production at Singosari National Insemination Center Ahmad Furqon; Irida Novianti; Wike Andre Septian; Rafika Febriani Putri; Chairdin Dwi Nugraha; Suyadi Suyadi
TERNAK TROPIKA Journal of Tropical Animal Production Vol 22, No 2 (2021): TERNAK TROPIKA Journal of Tropical Animal Production
Publisher : Jurusan Produksi Ternak, Fakultas Peternakan, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The study was aimed to evaluate semen production on different levels of breeds and ages. A total of 28 bulls were used in this study constisted of 13 Bali bulls, 6 Simmental bulls, and 9 Ongole Grade bulls. The bull’s age was ranged from 2 to 5 years old. The semen was collected at Singosari National Artificial Insemination Center three times a week during 2018. Bull’s semen was evaluated macroscopically and microscopically. Parameters analyzed in this study were semen volume, sperm motility and concentration. The result showed that the different semen quality was found among the bulls at different ages. Semen volume increased with time until 5 years genarally. Bali cattle had the lowest sperm concentration. Ongole Grade cattle had higher sperm motility and lower semen volume. Simmental had the lowest sperm motility. Simmental cattle had semen volume more than 6 mL. In conclusion, Ongole Grade cattle have higher semen production than Bali and Simmental. The failed semen collection was found among the bulls but the qualified semen production based on SNI would be used in the process of frozen semen at Singosari National Artificial Insemination Center
Co-Authors Ahmad Furqon Ahmad Furqon Ahmad Furqon Chairdin Dwi Nugraha Chairdin Dwi Nugraha Chairdin Dwi Nugraha Irida Novianti Nurgiartiningsih, V. M. A. S. Rahayu Suyadi Suyadi Suyadi Suyadi Wike andre Septian