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All Journal Jurnal Teknologi Dan Industri Pangan Zuriat Jurnal Hortikultura Buletin Penelitian Tanaman Rempah dan Obat BERITA BIOLOGI Buletin Penelitian Tanaman Rempah dan Obat
Ika Mariska
Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian Jl. Tentara Pelajar No.3A Cimanggu Bogor 16114
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Education Industrial & Manufacturing Engineering

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Characterization of B-glukosidase Enzyme from Vanilla Bean Dwi Setyaningsih; Maggy T Soehartono; Anton Apriyantono; Ika Mariska
Jurnal Teknologi dan Industri Pangan Vol. 18 No. 2 (2007): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (481.137 KB)

Abstract

The Indonesian natural vanilla is know for having a unigue woody, smooky, and phenolic flavor. Development of the aroma and flavor vanilla was formed by the action of a hydrolytic enzyme B-glucosidase on glucovanillin. The objective of this research was to characterize vanilla B-glucosidase. The vanilla B-glucosidase activity was increased by detergent. The enzyme was found as heat labile. Scalding should be conducted at 400C for 2-3 minutes. The result from B-glucosidase activity in each part of vanilla and microscopic analisis of vanilla bean slice showed that the highest B-glucosidase activity and vanillin concentrations were found in the seed funicles and placental tissue the of vanilla bean. The activity of vanilla B-glucosidase was optimum at pH 6,0, and temperature of 400C, found as and activation energy was 5,78 kcal/mole. After 44 minutes incubation time at 400C. The activity was reduced down to 10%. The apparent of moleculer weight was 100-400 kDa according to gel setration (Sephacryl S-300) analysis. Key words : Vanilla planifolia, B-glucosidase
OPTIMASI PROSES MASERASI VANILI (Vanilla planifolia Andrews) HASIL MODIFIKASI PROSES KURING [Maceration Process Optimation of Vanili (Vanilla Planifolia Andrews) from Modified Curing] Dwi Setyaningsih; Meika S Rusli; Melawati .; Ika Mariska
Jurnal Teknologi dan Industri Pangan Vol. 17 No. 2 (2006): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

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Abstract

Modified cured vanilla was processed to vanilla extract by maceration method. The aim of this research were to optimize the method of maceration, type of vanilla bean with highest vanillin content, extraction solvent composition,and other variables that could optimize the vanillin content and characterize the extract from half dried cured vanilla. The optimation used response surface method with 22 factorial and 23 factorial. One step of maceration could extract vanillin (average 2.3 g/l) much more than two steps maceration (average 2.1 g/l). Vanillin content of the half dried cured vanilla (average 0.98 g/l) was higher than cured vanilla 1 and cured vanilla 2 (average 0.41 g/l and 0.32 g/l). The suitable ethanol-water composition for half dried cured vanilla was 7:3 (vanillin content 1.78 g/l). The first optimation was conducted with two variables maceration time and sucrose concentrations. The maximum vanillin content of the first optimation was 4.5 g/l at maceration time of15.9 days and sucrose concentration of 7.3 g. The second optimation used two variables: maceration time and glycerol concentrations. The maximum vanillin content of the second optimation was 3.8 g/l at maceration time of 22 days and glycerol concentration 19.9 ml. The third optimation process used three variables:maceration time, sucrose concentrations and glycerol concentrations. The maximum vanillin content of the third optimation was 3.4 g/l at maceration time of 12 days sucrose concentration of 7 g, and glycerol concentration 4.7 ml. The characteristic of vanilla extract resulted from half dried cured vanilla maceration were vanillin content (3.4-4.5 g/l), total acid (380-410 ml 0.1 N NaOH/l), total ash (1.3-3.4 g/l), total soluble ash (0.8-2.9 g/l), alkalinity of total ash (462.6-536.7), alkalinity of soluble ash (139.1-216.5), and lead number (4.5-4.6). Key words : Vanilla planifolia, Optimization, vanilla ekstrak  
Regenerasi Tanaman Sedap Malam Melalui Organogenesis dan Embriogenesis Somatik Ika Rostika; Ika Mariska; R Purnamaningsih
Jurnal Hortikultura Vol 15, No 4 (2005): Desember 2005
Publisher : Indonesian Center for Horticulture Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jhort.v15n4.2005.p%p

Abstract

Secara konvensional perbanyakan tanaman sedap malam dilakukan melalui umbi. Semakin kecil ukuran umbi semakin lama tanaman berbunga. Penerapan teknik kultur in vitro diharapkan dapat membantu perbanyakan tanaman secara masal. Hingga saat ini, teknik kultur in vitro tanaman sedap malam belum pernah dilaporkan di Indonesia. Penelitian ini bertujuan memperoleh formulasi media yang efektif menginduksi organogenesis dan embriogenesis kultur in vitro tanaman sedap malam serta memacu regenerasinya. Percobaan dibagi menjadi 4 tahap, yaitu (1) induksi tunas, (2) multiplikasi tunas, (3) induksi kalus embriogenik, dan (4) regenerasi kalus embriogenik. Media induksi tunas yang diuji adalah MS+BA 0 ppm, MS+BA 3 ppm, MS+BA 5 ppm, dan MS+BA 7 ppm. Pemacuan multiplikasi tunas lanjut dilakukan pada media subkultur MS+BA 7 ppm+glutamin 100 ppm, MS+BA 7 ppm, DKW+TDZ 7 ppm, dan DKW+TDZ 7 ppm+glutamin 100 ppm. Untuk induksi kalus embriogenik, media induksi kalus yang diujikan adalah MS+2,4-D 2,5 ppm, MS +2,4-D 5 ppm, dan MS+2,4-D 10 ppm. Untuk meregenerasikan kalus embriogenik, media yang diujikan MS+BA 2 ppm+TDZ 0,2 ppm, MS+BA 3 ppm+TDZ 0,4 ppm, MS+zeatin 1ppm+kinetin 1ppm, dan MS+zeatin 0,5 ppm+kinetin 2 ppm. Hasil percobaan menunjukkan bahwa pembentukan tunas terbanyak diperoleh dari media BA 3 ppm (80%) namun inisiasi tunas tercepat dihasilkan pada media BA 0 ppm. Formula media MS+BA 7 ppm+glutamin 100 ppm menghasilkan jumlah tunas dan akar terbanyak. Penggunaan MS+2,4-D 5 ppm dapat menginduksi kalus embriogenik dengan persentase pembentukan nodul sebesar 18,75% dan jumlah nodul yang terbentuk sebanyak 3,6 dengan visual kalus yang paling baik. Setelah disubkultur, calon tunas terbanyak (17) dihasilkan dari perlakuan MS+BA 2 ppm+TDZ 0,4 ppm. Kalus embriogenik pada media MS+zeatin 0,5 ppm+kinetin 2 ppm dapat berkembang membentuk benih somatik.Regeneration of tuberose through organogenesis and embryogenesis. Tuberose is normally propagated by the tuber. The smaller size of tuber the longer time plant to flower. The application of in vitro culture technique might be used for mass propagation. Up to know, the research of in vitro culture of tuberose in Indonesia has not been reported. The objective of the study was to find out media formulation for organogenesis and embryogenesis. The experiments consisted of 4 steps of (1) shoot induction, (2) shoot multiplication, (3) induction of embryogenic callus, and (4) regeneration of embryogenic callus. The treatments for shoot induction were MS+BA 0 ppm, MS+BA 3 ppm, MS+BA 5 ppm, and MS+BA 7 ppm. The shoots were multiplied on media MS+BA 7ppm+glutamine 100ppm, MS+BA 7 ppm, DKW+TDZ 7 ppm, and DKW+TDZ 7 ppm+glutamin 100 ppm. For induction of embryogenic callus, the treatments were MS+2.4-D 2.5 ppm, MS+2,4-D 5 ppm, and MS+2.4-D 10 ppm. For regeneration of embryogenic callus, the treatments were MS+BA 2 ppm+TDZ 0.2 ppm, MS+BA 3 ppm +TDZ 0.4 ppm, MS+zeatin 1ppm+kinetin 1ppm, and MS+zeatin 0.5 ppm+kinetin 2 ppm. The results showed that the highest shoot formation was obtained from media MS+BA 3 ppm but the earliest shoot initiation was obtained from media MS+BA 0 ppm. The media formulation of MS+BA 7 ppm+glutamine 100 ppm gave the highest number of shoot and root. The application of media MS+2.4-D 5 ppm could induce embryogenic callus with high percentage of nodul formation (18.75%) and high number of nodul (3.6) with the best visual calli. After subculturing, the highest number of nodul (17) was obtained from media MS+BA 2 ppm+TDZ 0.4 ppm. The embryogenic callus from media MS+zeatin 0.5 ppm+kinetin 2 ppm could develop to form somatic seed.
Kultur Tunas Angelica acutiloba Melalui Teknik Kultur Jaringan Ika Mariska; Endang Gati
Buletin Penelitian Tanaman Rempah dan Obat Vol 2, No 1 (1987): Buletin Penelitian Tanaman Rempah dan Obat
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/bullittro.v2n1.1987.1-5

Abstract

Variabilitas Genetik Manggis Hasil Iradiasi Sinar Gamma Melalui Analisis RAPD Hamda Fauza; Murdaningsih H. Karmana; Neni Rostini; Ika Mariska
Zuriat Vol 14, No 2 (2003)
Publisher : Breeding Science Society of Indonesia (BSSI) / PERIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/zuriat.v14i2.6802

Abstract

Penelitian bertujuan untuk mengetahui variabilitas genetik tanaman manggis generasi M1 berdasarkan pola pita RAPD sebagai respon terhadap beberapa dosis irradiasi sinar gamma. Dosis iradiasi sinar gamma yang digunakan adalah 0 krad, 1 krad, 2 krad, dan 3 krad. Sumber bahan tanaman yang akan diiradiasi berasal dari biji buah manggis yang berasal dari satu tanaman. Hasil penelitian menunjukkan terdapat perbedaan variabilitas genetik di antara individu tanaman baik antar perlakuan maupun di dalam perlakuan. Perlakuan dengan dosis 1 krad memperlihatkan variabilitas genetik yang lebih luas disbanding perlakuan lain. Untuk mendapatkan variabilitas genetik yang luas disarankan menggunakan dosis iradiasi sinar gamma sebesar 1 krad.
PERTUMBUHAN DAN VARIABILITAS FENOTIPIK MANGGIS HASIL IRADIASI SINAR GAMMA Hamda Fauza; Murdaningsih H. Karmana; Neni Rostini; Ika Mariska
Zuriat Vol 16, No 2 (2005)
Publisher : Breeding Science Society of Indonesia (BSSI) / PERIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/zuriat.v16i2.6770

Abstract

Manggis (Garcinia mangostana L.) merupakan salah satu komoditas hortikultura buah-buahan tropik Indonesia, yang bernilai ekonomi tinggi. Manggis termasuk tanaman yang mengalami reproduksi secara apomiksis, sehingga variabilitasnya sempit. Mendapatkan jenis baru menggunakan metode pemuliaan tanaman konvensional dibatasi oleh sempitnya variabilitas genetik yang ada. Salah satu upaya yang dapat dilakukan untuk meningkatkan variabilitas genetik adalah melalui mutasi. Mutasi dapat dilakukan melalui induksi mutagen fisik, salah satunya dengan iradiasi sinar gamma. Penelitian bertujuan untuk mengetahui pertumbuhan dan variabilitas fenotipik tanaman manggis generasi M1 sebagai respon terhadap beberapa dosis  iradiasi sinar gamma. Dosis iradiasi sinar gamma yang digunakan adalah 0 krad (A), 1 krad (B), 2 krad (C), dan 3 krad (D). Percobaan di lapangan ditata dalam rancangan acak kelompok (RAK). Sumber bahan tanaman yang akan diiradiasi berasal dari biji buah manggis yang berasal dari satu tanaman. Hasil penelitian menunjukkan terdapat perbedaan variabilitas fenotipik di antara individu tanaman, baik antar perlakuan maupun di dalam perlakuan. Iradiasi sinar gamma dengan dosis 1 krad memperlihatkan pengaruh yang paling baik pada pertumbuhan tanaman di lapangan. Disarankan untuk melanjutkan penelitian ini ke tahap selanjutnya dalam melihat variabilitas genetik tanaman manggis dengan menggunakan marka DNA.
MIKROPROPAGASI SUKUN (Artocarpus communis Forst), TANAMAN SUMBER KARBOHIDRAT ALTERNATIF Yati Supriati; Ika Mariska; Sri Hutami
BERITA BIOLOGI Vol 7, No 4 (2005)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v7i4.1049

Abstract

Bread fruit (Artocarpus communis Forst) is one of tropical fruit, which has a high contain of carbohydrate.In certain area, it becomes an alternative staple food when the main staple foods are scarce.The amount of carbohydrate in breadfruit is almost the same with the one in sweet potato, but it is higher than in potato. The main constraint of the development of breadfruit is the limited of seedling availability.Tissue culture technique has been known for its excellent result for plant propagation, because this technique has ability in producing seedling in a large quantity, in uniform growth rate and in a relative short time.The experiment was conducted at Cell Tissue Culture Division, Indonesian Center Agricultural Biotechnology and Genetic Resource Research and Development (1CAB1OGRAD) from February 2003 until December 2004.There were some steps experiments with series of combination medium as treatments. The first steps was shoot multiplication at Sk-2 medium with WPM + BA (0; 0,5; 1,0; 1,5 and 2,0 mg/1) + Thidiazuron (0; 0,4 mg/1);The second step was elongation shoot at Sk-3 with WPM + kinetin (1,2 and 3 mg/1) + GAa(0 and 5 mg/1), and the third was root initiation and proliferation, by comparing WPM + IBA (0, 2, 4 and 6 mg/1) + charcoal (0;0,5 %) and WPM (1; 0,5) + BA (0; 1,5 and 5 mg.l) or NAA (1,2 and 3 mg/1). For the step of acclimatization, soil and compost were used in comparison of (1;1 and 1:2).The result showed that the best media for shoot multiplication of breadfruit was WPM + BA 2 mg/1 + TDZ 0 4 with shoot number of 15,5., while the best media for shoot elongation was WPM + Kinetin 1 mg/1 + GA, 5 mg/1.WPM + IBA 3 mg/1 was the best formula for root proliferation with the highest root number about 6.5 and percentage of shoot producing root about 60%. For acclimatization, soil and compost in combination of 1:1 was the best media for planlet of breadfruit with the success rate about 70%. Charcoal is not necessary in root initiation and proliferation.
Kultur Tunas Angelica acutiloba Melalui Teknik Kultur Jaringan Ika Mariska; Endang Gati
Buletin Penelitian Tanaman Rempah dan Obat Vol 2, No 1 (1987): Buletin Penelitian Tanaman Rempah dan Obat
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/bullittro.v2n1.1987.1-5

Abstract

Co-Authors Anton Apriyantono Dwi Setyaningsih Endang Gati Hamda Fauza Ika Rostika Maggy T Soehartono Meika S Rusli Melawati . Murdaningsih H. Karmana Neni Rostini RAGAPADMI PURNAMANINGSIH Sri Hutami Yati Supriati