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UJI DAYA HAMBAT EKSTRAK DAUN SIRSAK (Annona muricata L.) DAN DAUN SIRIH (Piper betle L.) TERHADAP PERTUMBUHAN BAKTERI Escherichia coli Eka Suci Fitria; Imran Imran; Sulastrianah Sulastrianah
MEDULA JURNAL ILMIAH FAKULLTAS KEDOKTERAN UNIVERSITAS HALU OLEO Vol 1, No 2 (2014)
Publisher : Halu Oleo University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (577.279 KB) | DOI: 10.46496/medula.v1i2.197

Abstract

Soursop (A. muricata L.) and betel (P. betle L.) are common plants that traditionally used as a medicine. The aims of this study are to determine the Minimum Inhibitory Concentration (MIC) of soursop leaves benefits and betel leaves extract that can inhibit the growth of E. coli one of the cause of diarrhea and the most potentially extract between that plants. Methode of this research used post test control only design and cotrimoxazole (as a control). This study consists of three phases : (1)sample preparation, (2)extraction by maceration with methanol and obtained as much as 14.97 g of leaf extract of A. muricata.L and 2.88 g of P. betle L. after evaporated, (3)MIC using the disc diffusion method. The concentration of each extract are 80,000 ppm, 40,000 ppm, 20,000 ppm, 10,000 ppm, 5000 ppm, 2500 ppm, and 1250 ppm. Ten μL of each extracts was taken and saturated to a paper disc. The results of this study showed that the A. muricata L leaf extract begin to show inhibition at concentration 2500 ppm and P. betle.L is at concentration 5.000 ppm. The conclusion of this study is the MIC of A. muricata L. and P. betle L.leaf extract are 2500 ppm and 5000 ppm sequentially.Key words: A. muricata L., P. betle L., Minimum Inhibitory concentration, E. coli
Bioaktifitas Antibakteri Ekstrak Etanol Teripang Pasir (Holothuria scabra) terhadap Pertumbuhan Klebsiella pneumoniae Secara In Vitro Fifit Ervitha; Sulastrianah Sulastrianah; Indria Hafizah
MEDULA JURNAL ILMIAH FAKULLTAS KEDOKTERAN UNIVERSITAS HALU OLEO Vol 3, No 1 (2015)
Publisher : Halu Oleo University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (397.561 KB) | DOI: 10.46496/medula.v3i1.2538

Abstract

Holothuria scabra is  one of the biota that commonly found in Southeast Sulawesi waters and traditionally use as a source food and medicine by local tribe. They also harvesting this species for exported to several countrywith high demand.  Even this species have commmercially beneficience, but there is lack of publication about the bioactivity, especially H. Scabra derived from southeast Sulawesi. This study aims to determine secunder metabolite that contain in the extractand to examine antibacterial bioactivity of H. scabra ethanol extract againstKlebsiellapneumoniae by defining the Minimum Inhibitory Consentration. The method using an experimental with post test onlycontrol group design. The extraction is using maseration method and the extract then  diluted and divided intoten concentration 100%, 50%, 25%, 12,5%, 6,25%, 3,125%, 1,56%, 0,78%, 0,39%, and 0,19%. The secunder metabolite was determined by using TLC and the MIC was determined by using dilution method. All consentration.As the result, TLC test showing positif result for alcaloid and triterpenoid and the nutrient broth starting to show no turbidity in concetration 0,78%. The conclusion of this study is ethanol extract of H. Scabra contain alkaloid and triterpenoid as secunder metabolite and the MIC was in 0,78% concentration.Keywords: Holothuria scabra, Klebsiella pneumoniae,  MIC, alkaloid, terpenoid
PERBEDAAN KEJADIAN INFEKSI CACING ANTARA PETUGAS PENGANGKUT SAMPAH YANG MENGGUNAAN ALAT PELINDUNG DIRI DENGAN PETUGAS PENGANGKUT SAMPAH YANG TIDAK MENGGUNAKAN ALAT PELINDUNG DIRI Lezdyana Nur Islami; Sulastrianah Sulastrianah; Wa Ode Sitti Asfiah Udu
MEDULA JURNAL ILMIAH FAKULLTAS KEDOKTERAN UNIVERSITAS HALU OLEO Vol 2, No 1 (2014)
Publisher : Halu Oleo University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (61.979 KB) | DOI: 10.46496/medula.v2i1.242

Abstract

Soil  Transmitted  Helminth  (STH)  infection  globally  spread  around  the  worlds.  Incidence  of  helminth infection  more  common  in  childrens,  but  adult  with  special  job  like  garbage  workers  have  same  risk  with  that childrens. The aim of this study is to determine differences between usage of personal protective equipment with the helminth  infection  among garbage  workers that  completely  used  personal  protective  equipement  and  that  not completely used . The method of this study is an observational with cross sectional design. Population in this study are garbage workers at Sanitary Service Wakatobi Regency. Sampling method using total sampling with 59 samples. The data about usage of personal protective equipement have done by observation and faecal examination by direct slide. The differences analysed statistically using Chi-Square test. As a result, it is obtained that among the workers that  not completely using  personal  protective  equipment,  there  27  (60%) samples  are infected  and 18  (40%) samples  are  not  infected.  Among  the  workers  that completely using  personal  protective  equipment, there  is  4 (28,6%) samples are infected and 10 (71,4%) samples are not infected (p = 0,04). The conclusion of this study is there was relation between usage of personal protective equipment with helminth infection in garbage workers. Key Words: Helminth infection, garbage workers, personal protective equipment
Gingival overgrowth following mechanical trauma during tooth preparation: a case report Sulastrianah Sulastrianah; Sri Oktawati
Journal of Dentomaxillofacial Science Vol. 4 No. 2 (2019): (Available online: 1 August 2019)
Publisher : DiscoverSys Inc.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15562/jdmfs.v0i0.749

Abstract

Objective: To explain the management of gingival overgrowth caused by mechanical trauma during tooth preparation.Method: In this case report, a patient experience gingival overgrowth one day after trauma during tooth preparation for bridge. The patient feel severe paint especially when the inflamed gingiva is bitten during occlusion. In intraoral examination overgrowth is localized red to pink white, firm nodule gingiva. Management of the patient is surgical removal of the overgrowth gingivaResults: After one week of operation, the gingiva shows a good healing and no recurrency.Conclusion: Gingival overgrowth may develop as a result from trauma or chronic irritation. The High speed rotary instrument may also make the trauma in the gingiva during tooth preparation. The carefully is needed when using this instrument.
Upaya Tanggap Bencana Nasional Melalui Edukasi dan Pencegahan Penyebaran Covid-19 Di Kota Baubau Jamaluddin Jamaluddin; Sufiah Asri Mulyawati; Zida Maulina Aini; Sulastrianah Sulastrianah; Raja Al Fath Widyaiswara
Jurnal Pengabdian Kedokteran Indonesia Vol 1 No 1 (2020): September 2020
Publisher : Fakultas Kedokteran Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1455.619 KB) | DOI: 10.33096/jpki.v1i1.104

Abstract

Kota Bau – Bau terletak pada selat Buton yang mempunyai aktivitas kelautan yang sangat tinggi, berperan sebagai titik transit sekaligus daerah penghubung bagi jalur nasional sekunder. Coronavirus adalah zoonosis atau virus yang ditularkan antara hewan dan manusia. Penyebaran penyakit ini telah memberikan dampak luas secara sosial dan ekonomi. Kegiatan ini bertujuan untuk mengembangkan pengetahuan dan pemahaman mengenai pencegahan dan penanganan covid-19. Metode pelaksanaan kegiatan meliputi kegiatan daring maupun luring seperti edukasi kepada masyarakat melalui video penyuluhan, pembagian masker, Hand sanitizer, dan pembagian Alat pelindung Diri. Kegiatan yang dilakukan dalam pengabdian ini melibatkan seluruh elemen masyarakat secara bersama-sama, bersinergi, multidisiplin, dan bermitra sehingga dapat membantu mengendalikan penyebaran Covid 19 dan menurunkan jumlah kasus diwilayah Kota Bau Bau. Program kerja yang dilaksanakan berdasarkan potensi dan permasalahan yang ada di Kota Bau Bau. Hasil dari pengabdian masyarakat ini berupa edukasi penanganan dan pencegahan Covid 19 dan penerapan protokol kesehatan di era new normal, pendistribusian masker kain, pendistribusian hand sanitizer, pembuatan sarana cuci tangan, serta pembagian alat pelindung diri bagi tenaga medis di kota Bau Bau. Capaian dan luaran kegiatan pengabdian kepada masyarakat meliputi peningkatan pengetahuan dan kesadaran masyarakat tentang pencegahan Covid 19 dan penerapan protokol kesehatan, peningkatan penerapan Pola Hidup Bersih dan Sehat, tersedianya Alat Pelindung Diri bagi tenaga kesehatan khususnya pada puskesmas dan rumah sakit.
ANTIBACTERIAL AND ANTIFUNGAL ACTIVITY OF BIOACTIVE COMPOUND FROM SEMELE CORDIFORMIS Sulastrianah, Sulastrianah; Fathan Rasyid; Clara M. Tumanan; Hartati, Hartati; I P. Sudayasa
Journal of Innovation Research and Knowledge Vol. 4 No. 2: Juli 2024
Publisher : Bajang Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53625/jirk.v4i2.8060

Abstract

Marine biodiversity of South East Sulawesi has been used by local communities for medication since a long time ago. One of which is Semele cordiformis or ‘tude bombang’ in local languages. The community believes that the hot water extract of S. cordifirmis can help to cure hepatitis. But this has not been proven scientifically. The purpose of this study is to evaluated antibacterial and antifungal activity of S. Cordiformis as a pilot project to explore the bioactivity of this biota. Method: Antibacterial activity was measured based on the Minimum Inhibitory Concentration (MIC) of S. cordiformis ethanol extract against Staphylococcus aureus ATCC 25923. Paper disks containing the extracts of S. cordiformis in several concentrations were placed on agar and the inhibition zones were measured. Antifungal activity was also measured based on the MIC of the S. cordiformis ethyl acetate extract against Candida albicans ATCC 10231. Each measurement is carried out in triplo. Result: Ethanol extract of S. cordiformis with concentrations of 50% and 100% showed strong inhibition responses (12.25 mm and 16.42 mm).Concentrations of S. cordiformis Ethyl acetate extract of 3000 mg/mL and 6000 mg/mL have moderate inhibition responses (9.58 mm and 5.42 mm). Conclusion: Semele cordiformis used in this study have potency as an antibacterial and antifungal. Further invastigation involving isolation of more specific bioactive compound of the extract need more research
The Immunization Status and Nutritional Knowledge Level of the Mother Influence the Nutritional Status of Toddlers in the Nambo Primary Health Care Working Area, Kendari City Milati Hamidah; Sulastrianah; Nina Indriyani Nasruddin
MEDULA: Jurnal Ilmiah Fakultas Kedokteran Universitas Halu Oleo Vol. 10 No. 2 (2023): April
Publisher : MEDULA: Jurnal Ilmiah Fakultas Kedokteran Universitas Halu Oleo

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Abstract

Background: Due to their golden age of physical development and intelligence, infants nutritional status requires special consideration. Preliminary investigations at the Nambo Primary Health Care found that 19 toddlers suffered from wasted, with four being severely wasted. Purpose: The purpose of this study is to examine the influence of the toddler's immunization status and the mother's level of nutritional education on the nutritional status of infants in the Nambo Primary Health Care working area. Methods: This study utilized a case-control design in the Nambo Primary Health Care working area from November to December 2022. This investigation included 69 toddlers as participants (23 toddlers in the case group and 46 toddlers in the control group). We gathered data by administering a questionnaire and measuring the toddler's weight and height, which we analyzed using the chi-square test and Fisher’s Exact test. Results: Results indicated that immunization status significantly influences the nutritional status of infants (OR = 4.594; p = 0.034; 95% CI: 1.183–17.840). The mother's nutritional knowledge has a significant effect on the nutritional status of toddlers (OR = 10.587; p = 0.000; 95% CI = 3.118–37.818). Conclusions: The nutritional status of toddlers in the Nambo Primary Health Care Working Area, Kendari City, is influenced by the nutritional knowledge and vaccination status of their mothers.
Correlation between Knowledge and Hesitancy towards COVID-19 Vaccine in Pregnant Women in The Working Area of Ranomeeto Health Centre Yusril Marhaen; Juminten Saimin; Sulastrianah
MEDULA: Jurnal Ilmiah Fakultas Kedokteran Universitas Halu Oleo Vol. 10 No. 3 (2023): Suplemen
Publisher : MEDULA: Jurnal Ilmiah Fakultas Kedokteran Universitas Halu Oleo

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Abstract

Background: Acceptance or refusal of vaccinations can be influenced by hesitancy about the COVID-19 vaccine. Hesitancy is determined by knowledge, attitudes, practices, and public concerns about the safety, efficacy, risks, and benefits associated with the COVID-19 vaccination program. Purpose: To determine the association between knowledge and hesitancy about the COVID-19 vaccine in pregnant women in the Ranomeeto Health Center Working Area. Methods: This is an analytic observational study with a cross-sectional approach. This study was conducted at the Ranomeeto Health Center, Konawe Selatan District. The number of samples is 77 people, collected by purposive sampling technique. Knowledge was measured using a knowledge questionnaire about the covid-19 vaccine. Hesitation was measured using a questionnaire about the COVID-19 vaccine. Data were collected and analyzed using the Chi-Square statistical test with a significance value of p<0.05. Results: The results showed that most pregnant women had good knowledge about the COVID-19 vaccine (42.9%) and had no hecitation about the COVID-19 vaccine (61.0%). Statistical tests obtained a significant p-value between knowledge and hesitancy about the COVID-19 vaccine (p=0.010). Conclusion: There is a significant correlation between knowledge and hesitancy about the COVID-19 vaccine in pregnant women in the Working Area of the Ranomeeto Health Center.
Correlation Of Age And BMI With The Incident Of Nausea And Vomiting In Patients Post Sectio Caesarian Operation With Spinal Anesthesia In Aliyah Hospital 2 Agussalim Ali; Parawansah; Sulastrianah
MEDULA: Jurnal Ilmiah Fakultas Kedokteran Universitas Halu Oleo Vol. 10 No. 3 (2023): Suplemen
Publisher : MEDULA: Jurnal Ilmiah Fakultas Kedokteran Universitas Halu Oleo

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Abstract

Background. Postoperative nausea and vomiting are one of the complications experienced by patients with anesthesia and surgery. There are many factors that influence this occurrence, including age and BMI, where young age and obese BMI tend to experience postoperative nausea and vomiting. This is based on the fact that older age is easier to control nausea and vomiting than young people and obese BMI has more fat tissue to store anesthetic drugs so that the side effects of drugs can last longer. Method. This study used an observational analytic method with a cross sectional approach carried out at Aliyah Hospital 2. The number of samples in this study were 38 people who were taken using total sampling technique. Data collection was obtained from filling in the observation sheet and then the data was analyzed using the Fisher's Exact Test statistical test. The dependent variable in this study was postoperative nausea and vomiting and the independent variables were age and BMI. Results. The results of this study found that age did not have a significant relationship with the incidence of postoperative nausea and vomiting at Aliyah Hospital 2 (p = 0.298) and BMI had a significant relationship with the incidence of postoperative nausea and vomiting at Aliyah Hospital 2 (p = 0.019). Conclusion. From this study it can be concluded that the incidence of postoperative nausea and vomiting after spinal anesthesia is higher in patients with older age and excessive BMI.
Anti-inflammatory Activity of Kappaphycus Alvarezii Using Protein Denaturation Inhibition Method Windy Zafa Amalia; Sulastrianah; Arimaswati
MEDULA: Jurnal Ilmiah Fakultas Kedokteran Universitas Halu Oleo Vol. 11 No. 2 (2024)
Publisher : MEDULA: Jurnal Ilmiah Fakultas Kedokteran Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46496/medula.v11i2.37

Abstract

ABSTRACT Background: Inflammation is the body's reaction to injury or tissue damage. As an NSAID, diclofenac sodium is frequently used. But since this medication has adverse effects, a substitute is required. Flavonoids found in K. alvarezii may have therapeutic uses and anti-inflammatory potential. Purpose: The goal of this research was to investigate the anti-inflammatory effect of K. alvarezii utilizing the protein denaturation inhibition method. Method: The IC50 was utilized to test anti-inflammatory effect through protein denaturation inhibition. Diclofenac sodium served as a positive control. The data were then analyzed with an independent t-test. Result: The anti-inflammatory activity test produced an IC50 of 197.94 ppm for extract K. alvarezii and 35.7 ppm for diclofenac sodium. The statistical test found a significant difference between seaweed ethanol extract K. alvarezii and sodium diclofenac (p = 0.000). Keywords: anti-inflammatory; Kappaphycus alvarezii; protein denaturation inhibition.