Garuda - Garba Rujukan Digital

p-Index From 2021 - 2026

0.659

P-Index

This Author published in this journals

All Journal Jurnal Teknologi Dan Industri Pangan Jurnal Pengolahan Hasil Perikanan Indonesia Jurnal Natur Indonesia Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Majalah Kulit, Karet, dan Plastik agriTECH Indonesian Food and Nutrition Progress Biosaintifika: Journal of Biology & Biology Education Jurnal Pengelolaan Laboratorium Pendidikan Buletin Anatomi dan Fisiologi (Bulletin of Anatomy and Physiology) Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan

Indrati, Retno

Department Of Food And Agricultural Product Technology, Faculty Of Agricultural Technology, Universitas Gadjah Mada, Jl. Flora No.1 Bulaksumur, Yogyakarta, Indonesia 55281

Author-ID : 2869793

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Engineering Environmental Science Health Professions Industrial & Manufacturing Engineering Medicine & Pharmacology Public Health

Published : 31 Documents Claim Missing Document

Claim Missing Document

Articles

Title

1 2

Isolasi dan Seleksi Jamur Perusak Karet dari Berbagai Sumber Kontaminasi Potensial Maria Ulfah; Purnomo Darmadji; Retno Indrati
agriTECH Vol 21, No 4 (2001)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Ribbed smoked sheet (RSS) is Natural rubber product, mainly consist of Cis- 1,4 polyisoprene and relatively resistant to microbial decomposition with many other natural polymer. Natural rubber contains a minimum of 90% rubber hydrocarbon, plus small amounts of protein, resins, fatty acid, sugars and minerals. It is also possible that microorganisms using impurities as their carbon and energy sources could deteoriorate the rubber. Fungi which potentially deteriorate rubber eventually was accidentally taken by raw material areas, raw materials, processing steps, product and old RSS. Therefore it is necessary to isolate fungi and to evaluate potential fungi which deteriorated rubber. The results showed that 158 strains were isolated from various contaminant sources. From these 10 strains were from skin of rubber wood, 7 strains from tappinglump, 16 strains from spoutlump and 10 strains from cuplump, 47 strains from earth around rubber wood, 5 strains from fresh field latex, 3 strains from ammoniated field latex, 12 strains from wet sheet, 7 strains from air in wet in sorting room, 12 strains from RSS product, 11 strains from old RSS product (7 years old namely SU) and 8 strains from wet SU (1 month old namely SDU) Twenty four strains were selected as potential deteriorated rubber fungi. These 24 strains were classified into 10 genera (Aspergillus, Cladosporium, Emericella, Fusarium, Mucor, Neosartorya, Penicillium, Pestalotiopsis, Cladobotyrum, Pithomyces), and 4 unidentified strains (LM188, T55, SS37, T67).

Sifat-Sifat Enzime Lipase Amobil dari Rhizopus delemar dalam Membran Polipropilen Retno Indrati; Djagal W. Marseno; Yoshiyuka Ohta
agriTECH Vol 19, No 4 (1999)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Evaluasi Perlakuan Pendahuluan Menggunakan Kalsium Hidroksida untuk Biokonversi Jerami Padi Menjadi L-Asam Laktat oleh Rhizopus oryzae AT3 Dhina Aprilia Nurani Widyahapsari; Retno Indrati; Sigit Setyabudi; Sardjono Sardjono
agriTECH Vol 36, No 3 (2016)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

L-lactic acid can be used as a precursor of polylactic acid (PLA). PLA is a biodegradable biomaterial commonly used for biodegradable plastics. Lactic acid can be produced from lignocelluloses materials such as rice straw. Rice straw is composed of cellulose and hemicellulose that can be hydrolyzed to fermentable sugar by cellulolytic and hemicellulolytic enzymes then converted to L-lactic acid by Rhizopus oryzae. As most cellulose and hemicellulose present in lignocellulose biomass are not readily accessible for these enzyme, pretreatment is required to alter the structure of lignocellulose substrates. This research aimed to investigate the effect of lime pretreatment on rice straw bioconversion to L-lactic acid by Rhizopus oryzae AT3. Rice straw was pretreated with lime (Ca(OH)2) at 85 °C for 16 hours. Unpretreated and pretreated rice straw were hydrolyzed using crude enzyme that produced by Trichoderma reesei Pk1J2. Enzyme production was carried out by solid state fermentation using rice straw and rice brand as substrate. Enzymatic hydrolysis was carried out in flasks. Each flask was added with unpretreated or pretreated rice straw, buffer citrate solution and crude enzyme then hydrolyzed for 0-96 hours. Hydrolysate was fermented by Rhizopus oryzae AT3 for 0-6 days by using adsorbed carrier solid-state fermentation method with polyurethane foam as inert support material. Lime pretreatment at 85 °C for 16 hour led to significant solubilisation of lignin and hemicellulose. It involved lignocellulose structure modified that enhance enzymatic hydrolysis and resulted higher reducing sugars than unpretreated rice straw. The high reducing sugars was not related to high lactic acid yields. Fermentation of pretreated rice straw hydrolysate by Rhizopus oryzae AT3 did not only produce L-lactic acid but also other compound. On the other hand, fermentation of unpretreated rice straw hydrolysate only produced L-lactic acid. ABSTRAKPolimerisasi asam laktat menjadi polylactic acid untuk menghasilkan biodegradable plastic membutuhkan asam laktat dengan isomer spesifik. Rhizopus oryzae adalah mikroorganisme yang spesifik menghasilkan L-asam laktat. Selain itu Rhizopus oryzae dapat menggunakan limbah pertanian seperti jerami padi sebagai substrat. Komponen utama jerami padi merupakan lignoselulosa yang dapat dihidrolisa secara enzimatis menjadi komponen gula sederhana penyusunnya dan selanjutnya dapat dikonversi menjadi L-asam laktat oleh Rhizopus oryzae. Namun struktur lignoselulosa sangat kompak dan rapat, sulit untuk dihidrolisa secara enzimatis sehingga diperlukan adanya perlakuan pendahuluan untuk merombak struktur lignoselulosa agar mudah dihidrolisa. Penelitian ini bertujuan untuk mengetahui pengaruh perlakuan pendahuluan menggunakan kalsium hidroksida (Ca(OH)2) terhadap biokonversi jerami padi menjadi L-asam laktat oleh Rhizopus oryzae AT3. Perlakuan pendahuluan pada jerami padi dilakukan menggunakan (Ca(OH)2) disertai pemanasan suhu 85 °C selama 16 jam. Jerami padi dengan dan tanpa perlakuan pendahuluan dihidrolisa secara enzimatis menggunakan crude enzyme yang diproduksi oleh Trichoderma reesei Pk1J2. Produksi crude enzyme dilakukan dengan fermentasi substrat padat dengan campuran jerami padi dan dedak sebagai substrat. Hidrolisat jerami padi dengan dan tanpa perlakuan pendahuluan selanjutnya difermentasi oleh Rhizopus oryzae AT3 menggunakan metode adsorbed carrier solid state fermentation dengan polyurethane foam (PUF) sebagai bahan pendukung. Perlakuan pendahuluan menggunakan Ca(OH2) disertai pemanasan suhu 85 °C selama 16 jam dapat merubah komposisi lignoselulosa jerami padi yaitu dengan melarutkan lignin dan hemiselulosa. Perubahan komposisi lignoselulosa memudahkan kerja crude enzyme dalam menghidrolisa jerami padi sehingga menghasilkan gula reduksi lebih tinggi dibandingkan jerami padi tanpa perlakuan pendahuluan. Tingginya gula reduksi tidak serta merta meningkatkan yield L-asam laktat yang dihasilkan. Fermentasi hidrolisat jerami padi dengan perlakuan pendahuluan oleh Rhizopus oryzae AT3 menghasilkan yield L-asam laktat lebih rendah dibandingkan hidrolisat jerami padi tanpa perlakuan pendahuluan. Namun pada jerami padi dengan perlakuan pendahuluan dihasilkan senyawa lain selain asam laktat.

Skrining Lactobacillus plantarum Penghasil Asam Laktat untuk Fermentasi Mocaf Zulafa Noor; Muhammad Nur Cahyanto; Retno Indrati; Sardjono Sardjono
agriTECH Vol 37, No 4 (2017)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

This study was aimed to select the best isolates of Lactobacillus plantarum from 6 (six) of local isolates obtained from culture collections isolated from traditional foods. Selection of isolates was based on the growth rate, the number of cells, changes in pH and lactic acid production during cell growth on the MRS-broth at 37 °C for 24 hours. The results showed that the growth rate of each isolate varied, as measured by the length of the log phase, ranging from 8-20 h. It showed that some isolates quite fast towards a stationary phase and some quite slow. The number of cell ranged from 8.81 to 9.74 log CFU/mL, while the pH at the beginning of cell growth from 5.2 to 5.8, and at the end of growth from 3.4 to 3.7. Lactic acid production by the end of the growth (24 h) is 0.76 to 0.98%. The results showed isolate of L. plantarum UA3 was best having the fastest growth rate (8 h of log phase), the highest cell number (9.74 log CFU/mL), and the highest lactic acid produced (0.92 %) for 14 h incubation on MRS-broth at 37 °C. Application of selected isolate of L. plantarum UA3 on solid substrate fermentation using media grated cassava yield in 0.92% lactic acid after fermentation for 60 h, with a cell number of 9.54 log CFU/mL. ABSTRAKPenelitian ini bertujuan untuk memilih isolat Lactobacillus plantarum terbaik dari 6 (enam) macam isolat lokal yang diperoleh dari koleksi kultur yang berasal dari makanan tradisional. Pemilihan isolat didasarkan pada kecepatan pertumbuhan, jumlah sel, perubahan pH dan produksi asam laktat selama pertumbuhan pada media MRS-broth pada suhu 37 °C selama 24 jam. Hasil penelitian menunjukkan bahwa kecepatan pertumbuhan setiap isolat bervariasi, yang diukur dari lamanya fase log, yaitu berkisar antara 8–20 jam. Ini memperlihatkan bahwa beberapa isolat cukup cepat menuju fase stasioner dan beberapa lagi cukup lambat. Jumlah sel berkisar antara 8,81–9,74 log CFU/mL, sedangkan pH pada awal pertumbuhan sekitar 5,2–5,8, dan pada akhir pertumbuhan 3,4–3,7. Produksi asam laktat pada akhir pertumbuhan (24 jam) adalah 0,76–0,98%. Dari hasil tersebut isolat L. plantarum UA3 merupakan isolat terbaik dengan fase pertumbuhan log yang tercepat (8 jam), jumlah sel tertinggi (9,74 log CFU/mL), dan menghasilkan asam laktat paling tinggi (0,92%) pada inkubasi selama 14 jam dalam media MRS-broth suhu 37 °C. Aplikasi dari isolat terpilih L.plantarum UA3 dalam fermentasi substrat padat menggunakan media kasava parut menghasilkan asam laktat sebesar 0,92% setelah fermentasi selama 60 jam, dengan jumlah sel 9,54 logCFU/mL.

Tempe Lamtoro (Leucena leucocephala), Pengaruhnya Terhadap Absorpsi Zat Besi Pada Tikus Mary Astuty; Retno Indrati; Yotty Atmajaya
agriTECH Vol 8, No 1 (1988)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Isolasi Bakteri Penghasil Enzim Lipase Penghasil Alkali dari Kulit Hewan V.S. Pertiwi Rumiyati; Retno Indrati
agriTECH Vol 18, No 2 (1998)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Twenty seven bacterial strains isolated an a medium containing 0.50% palm oil as substrate and 0.015% spirit blue as an indicator from several raw hides of chicken, goat and cow showed an extracellular lipase activity. Of these strains, 10 were higher lipase producers. Lipases produced from five of these 10 isolates were stable on alkaline conditions, ranging from pH 8.0 to 11.0. Furthermore, activity of these enzymes was not inhibited by the presence of non-ionic, cationic nor ionic surfactants at concentration of 0.05% or 0.10%. Conversely, some of these surfactans could even activate the enzyme.

Pola Perubahan Protein Koro Benguk (Mucuna pruriens) Selama Fermentasi Tempe Menggunakan Inokulum Raprima Novia Aristi Rahayu; Muhammad Nur Cahyanto; Retno Indrati
agriTECH Vol 39, No 2 (2019)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Tempe is a nutritious healthy food because it contains bioactive compounds that are beneficial to human health. This product is good for those who are vegetarian. During the fermentation process, fungi produce proteases that break down the velvet bean’s proteins into protein fragments or peptides which have functional properties. The fungus strain and the duration of incubation time will affect the bioactive peptides formed. This study aimed to determine the effect of tempe inoculum on changes in peptide concentration and protein patterns during fermentation. The results showed that proteolytic activity increased rapidly at the beginning of tempe fermentation and reached its optimum activity in 96 h fermentation period (0.046 U/mL). The pH of tempe changed from 7.01 then decreased to 5.92 in 30 h incubation, after that it increased again to 7.25 at the end of fermentation time (120 h). Peptide concentration increased with increasing fermentation time. The degree of hydrolysis increased rapidly until 24 h of incubation, then began to be stable until 96 h of incubation (reaching the hydrolysis degree of 46.31%). SDS PAGE patterns showed the formation of proteins/peptides with a molecular weight of <25 kDa as a result of hydrolysis of velvet bean protein during tempe fermentation using Raprima inoculum

Emulsifying Characteristics of Gelatin Hydrolysate from Tilapia Skin Covalently Attached with N-hydroxysuccinimide Esters of Fatty Acids Nurin Imana Hidayati; Retno Indrati; Pudji Hastuti; Masahiro Ogawa
agriTECH Vol 40, No 1 (2020)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

This study aims to combine two modification methods, namely enzymatic hydrolysis and covalent attachment with hydrophobic groups, to increase the emulsifying properties of gelatin. The experiment was conducted by using a completely randomized design with three replicates. Enzymatic hydrolysis of gelatin resulted in higher contents of free amino groups, which could be attached to hydrophobic groups. Gelatin hydrolysates covalently attached with the N-hydroxysuccinimide esters of C14:0 and C18:0 fatty acids at a molar ratio of 3.0 showed high emulsifying activity but low stability. Among the samples obtained, gelatin hydrolysate covalently attached with C18:0 at a molar ratio of 3.0 revealed the highest emulsifying activity; however, this sample cannot be considered the best emulsifier among the samples because of its low stability.

Microbial Conversion of Rice Straw Into Lactic Acid Through Simultaneous and Separate Hydrolysis and Fermentation Yuliana, Eva; Indrati, Retno; Setyabudi, Francis Maria Constance Sigit; Ningsih, Dewi Pujo; Sardjono, Sardjono
agriTECH Vol 45, No 2 (2025)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/agritech.81924

Lactic acid is often produced from lignocellulosic materials through various steps, including pretreatment, hydrolysis, and fermentation. Therefore, this study aims to evaluate the conversion of lactic acid from rice straw using simultaneous and separate hydrolysis and fermentation. The process was initiated with and without pretreatment using Ca(OH)2 at 85 °C for 16 hours, followed by hydrolysis using Trichoderma reesei PK1J2 and fermentation with Rhizopus oryzae AT3. Hydrolysis and fermentation were performed simultaneously and separately. The results showed that pretreatment could reduce lignin content, but this process was not needed because Trichoderma reesei PK1J2 degraded lignin during hydrolysis. In addition, fermentation conditions in this study could not support the production of lactic acid by Rhizopus oryzae AT3. Based on biomass growth during the treatment, simultaneous hydrolysis and fermentation (108 mg/g dry substrate) was better compared to separate hydrolysis and fermentation (104 mg/g dry substrate).

Co-Authors Agnes Murdiati Bagus Fajar Pamungkas Bayu Kanetro Bayu Kanetro Bayu Kanetro Djagal W. Marseno Djagal Wiseso Marseno Djagal Wiseso Marseno Djagal Wiseso Marseno Endang S. Rahayu Hari Eko Irianto I Dewa Gde Mayun Permana Kapti Rahayu Kuswanto Kapti Rahayu Kuswanto Maria Ulfah Marta Tika Handayani Marwati Mary Astuty Masahiro Ogawa Muhammad Halim Muhammad Nur Cahyanto Ningsih, Dewi Pujo Nurin Imana Hidayati Pramesti Dewi Pudji Hastuti Pudji Hastuti Purnama Darmadji Purnomo Darmadji Rachma, Yasmin Aulia Rahayu, Novia Aristi Ria Millati Sardjono Sardjono Sardjono Sardjono Sardjono Sardjono Sardjono Sardjono Setyabudi, Francis M.C. Sigit Sigit Setyabudi Suci Apsari Pebrianti Suparmo Suparmo Supriyadi Supriyadi Supriyadi Supriyadi Suryanti Suryanti Suryanti Suryanti Sutardi Sutardi Sutardi Sutardi Sutardi Sutardi Titik Kurniawati Tranggono . Tranggono Tranggono Tyas Utami Tyas Utami V. Sri Pertiwi Rumiyati, V. Sri Pertiwi V.S. Pertiwi Rumiyati Widyahapsari, Dhina Aprilia Nurani Wisnu Adi Yulianto Yastanto, Anang Juni Yoshiyuka Ohta Yotty Atmajaya Yuliana, Eva Yusmarini Yusmarini Yustinus Marsono Yustinus Marsono Zuheid Noor Zuheid Noor Zuheid Noor Zuheid Noor Zulafa Noor

Title Search

Found 19 Documents Search Journal : agriTECH

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 19 Documents
Search
Journal : agriTECH