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POTENSI HERBAL BUAH MAHKOTA DEWA (Phaleria Macrocarpa (scheff.) Boerl) YANG DIMANFAATKAN SEBAGAI MODIFIKATOR PERMUKAAN DAN ANTI-ADHESI BAKTERI S.mutans PADA PERMUKAAN MATERIAL RESTORASI RESIN KOMPOSIT : POTENTION OF PHALEVIA FRUIT WICH WAS BE BENEFITTED AS A SURFACE MODIFICATOR AND ANTI ADHESIANT OF S.mutans BACTERIA ON THE SURFACE OF RESTORATION COMPOSITE RESIN MATERIAL Heribertus Dedy Kusuma Yulianto; Morita
Dentika: Dental Journal Vol. 18 No. 2 (2014): Dentika Dental Journal
Publisher : TALENTA

Secara alamiah, bakteri rongga mulut mempunyai kecenderungan untuk melekat pada permukaan jaringan keras danlunak rongga mulut karena ketersediaan makanan untuk bakteri terakumulasi di permukaan. Kondisi patologis yangditimbulkan oleh perlekatan bakteri pada permukaan menjadi perhatian khusus dan menjadi objektif penelitian ini, yaituuntuk mencari metode yang efektif dalam menghambat perlekatan bakteri. Metode yang diteliti adalah metode pelapisanpermukaan/coating pada permukaan material restorasi RK yang bertujuan untuk memodifikasi sifat permukaan (surfacetreatment) material restorasi RK yang cenderung bersifat hidrofobik. Sifat hidrofobik ini ditengarai menjadi salah satufaktor penting yang berkontribusi terhadap perlekatan bakteri. Dengan melakukan modifikasi permukaan diharapkandapat mengurangi kemampuan perlekatan bakteri pada permukaan restorasi RK. Indikator yang digunakan untukmengamati perubahan sifat hidrofobisitas permukaan adalah perubahan sudut kontak (contact angle) cairan yangditeteskan pada permukaan. Sudut kontak permukaan yang lebih kecil mengindikasikan permukaan yang bersifat lebihhidrofilik. Untuk mengevaluasi efektivitas metode pelapisan pada permukaan yang bertujuan untuk memodifikasi sifatpermukaan, digunakan senyawa berbentuk cairan yang diekstrak dari buah mahkota dewa (phaleria macrocarpa (scheff.)boerl). Pengaruh permukaan yang dilapisi dengan cairan ekstraksi terhadap perlekatan bakteri diuji dengan menggunakanalat Scanning Electron Microscopy (SEM). Hasil penelitian menunjukkan metode pelapisan dengan cairan ekstrak buah mahkota dewa mampu menurunkan sifat hidrofobisitas permukaan RK, dan hasil gambaran SEM secara kualitatifmenunjukkan adanya penghambatan pembentukan koloni bakteri S.mutans. pada permukaan yang dilapisi dengan cairanekstrak buah mahkota dewa.

Dealing with the high-risk potential of COVID-19 cross-infection in dental practice Yulianto, Heribertus Dedy Kusuma; Purwanti, Nunuk; Utami, Trianna Wahyu; Dewi, Anne Handrini; Listyarifah, Dyah; Ruspita, Intan; Nur, Asikin; Susilowati, Heni
Majalah Kedokteran Gigi Indonesia Vol 6, No 1 (2020): April
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.56588

The World Health Organization reported that the SARS-CoV-2 virus has infected more than 5 million people around the world. Dental care providers and health care professionals need to be aware of the high-risk potential of crossinfection since the routes of virus transmission commonly happen through droplets and aerosols. This review aimed at collecting essential knowledge about the COVID-19 needed by dental practitioners. The review focused on the oral involvement in COVID-19, the role of oral transmission as the high-risk potential of cross-infection and recommended strategies to minimize the risk of cross-infection in dental practice. We searched all the published clinical features from PubMed, Google Scholar, Scopus and hand searched library online databases, from January 2015 until May 2020. Keywords used were â€œCOVID-19â€, â€œDentistryâ€, â€œDental protectionâ€, â€œCross-contaminationâ€, â€œAerosol and non aerosolâ€, and â€Povidone-iodineâ€ with their combinations. We identified 52 articles to review after the initial selection with inclusion and exclusion criteria. Results showed use of topical applications of povidine-iodine and viricidal mouthwash could significantly reduce the high-risk of cross-infection from dentistry patients who are asymptomatic with COVID-19 infection. Further safeguards include suspending all non-emergency procedures temporarily and closely screening patients for symptoms which may be suspected to be COVID-19 infection.

Dealing with the high-risk potential of COVID-19 cross-infection in dental practice Heribertus Dedy Kusuma Yulianto; Nunuk Purwanti; Trianna Wahyu Utami; Anne Handrini Dewi; Dyah Listyarifah; Intan Ruspita; Asikin Nur; Heni Susilowati
Majalah Kedokteran Gigi Indonesia Vol 6, No 1 (2020): April
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.56588

The World Health Organization reported that the SARS-CoV-2 virus has infected more than 5 million people around the world. Dental care providers and health care professionals need to be aware of the high-risk potential of crossinfection since the routes of virus transmission commonly happen through droplets and aerosols. This review aimed at collecting essential knowledge about the COVID-19 needed by dental practitioners. The review focused on the oral involvement in COVID-19, the role of oral transmission as the high-risk potential of cross-infection and recommended strategies to minimize the risk of cross-infection in dental practice. We searched all the published clinical features from PubMed, Google Scholar, Scopus and hand searched library online databases, from January 2015 until May 2020. Keywords used were “COVID-19”, “Dentistry”, “Dental protection”, “Cross-contamination”, “Aerosol and non aerosol”, and ”Povidone-iodine” with their combinations. We identified 52 articles to review after the initial selection with inclusion and exclusion criteria. Results showed use of topical applications of povidine-iodine and viricidal mouthwash could significantly reduce the high-risk of cross-infection from dentistry patients who are asymptomatic with COVID-19 infection. Further safeguards include suspending all non-emergency procedures temporarily and closely screening patients for symptoms which may be suspected to be COVID-19 infection.

RONGGA MULUT SEBAGAI RESERVOIR POTENSIAL UNTUK INFEKSI Pseudomonas aeruginosa Puspita Hajardhini; Heni Susilowati; Heribertus Dedy Kusuma Yulianto
ODONTO : Dental Journal Vol 7, No 2 (2020): December 2020
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.7.2.125-133

Introduction : Pseudomonas aeruginosa (P. aeruginosa) is Gram-negative bacteria which is normally inhabiting in environment, however it tends to be an opportunistic pathogen within oral cavity. It utilizes the oral cavity as potential reservoir to infect either lungs or oral cavity itself. Both planktonic and biofilm forms can mediate its infection in oral cavity so that making its difficulties to eradicate since its broad resistance to antibiotics.Method : We review the pathogenesis of oral infection and host defense mechanism to P. aeruginosa as well.Discussion: Several prevention strategies, both chemical and nonchemical, are elaborated to avoid oral bacterial contamination.Conclusion : Prevention strategies in dental aspect to P. aeruginosa is highly needed as its occurrence are susceptible to cross-infection.

Potensi Lendir Lele (Clarias batrachus) sebagai Saliva Buatan untuk Perawatan Mulut Kering Zipora Silka Yoretina; Deaoxi Renaschantika Djatumurti; Roissatun Nasikah; Hendri Susanto; Heribertus Dedy Kusuma Yulianto
Jurnal Perikanan Universitas Gadjah Mada Vol 23, No 2 (2021)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.62569

Xerostomia merupakan kondisi klinis pasien berupa mulut kering yang dapat meningkakan pertumbuhan jamur Candida albicans penyebab kandidiasis. Penanganan pasien xerostomia dapat dilakukakan dengan pemberian saliva buatan. Lendir lele memiliki karakristik dan kemampuan dalam mengganti fungsi saliva sekaligus sebagai antijamur Candida albicans. Penelitian ini bertujuan untuk mengetahui potensi lendir lele (Clarias batrachus) sebagai saliva buatan untuk perawatan mulut kering. Lendir lele (Clarias batrachus) diekstraksi kemudian diformulasikan menjadi saliva buatan dengan konsentrasi 13,2%, 14,4%, dan 17,2%. Pengujian dilakukan dengan mengukur tegangan permukaan menggunakan indikator besarnya sudut kontak tiap tetesan pada glass slide, mengukur derajat keasaman saliva buatan dengan pH meter, serta uji daya hambat pertumbuhan Candida albicans dengan metode disc-diffusion. Data kemudian dianalisis menggunakan uji ANOVA satu jalur dan LSD dengan tingkat kepercayaan 95%. Uji ANOVA menunjukkan perbedaan sudut kontak antara saliva buatan 13,2%, 14,4% dan 17,2% pada glass slide dengan saliva alami dan kontrol positif (p<0.05), Perbedaan daya hambat terhadap koloni Candida albicans signifikan antara saliva buatan dengan kontrol positif dan saliva alami (p<0.05). Berdasarkan uji pH, terdapat perbedaan signifikan ketiga konsentrasi saliva buatan (13,2; 14,4; 17,2%) dengan saliva alami dan kontrol positif. Lendir lele berpotensi sebagai bahan pembuatan saliva buatan dan dapat berfungsi sebagai anti-jamur (Candida albicans).

Kemampuan Pembasahan Saliva Buatan dengan Kandungan Ekstrak Mukus Lele (Clarias batrachus) Zipora Silka Yoretina; Anne Handrini Dewi; Hendri Susanto; Heribertus Dedy Kusuma Yulianto
Jurnal Perikanan Universitas Gadjah Mada Vol 23, No 2 (2021)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.62571

Xerostomia merupakan keluhan subyektif mulut kering yang sering berhubungan dengan adanya penurunan fungsi kelenjar saliva. Penanganan pasien xerostomia dapat dilakukan dengan pemberian saliva buatan. Mukus lele memiliki karakteristik dan kemampuan dalam menggantikan fungsi saliva, sehingga dengan konsentrasi yang tepat mampu berperan sebagai lubrikan yang baik. Penelitian ini bertujuan untuk mengetahui ada tidaknya kemampuan pembasahan antara saliva manusia dengan saliva buatan yang mengandung mukus lele (Clarias batrachus) pada permukaan hidrofobik. Mukus lele (Clarias batrachus) diekstraksi kemudian diformulasikan menjadi saliva buatan dengan menambahkan 0,1% methylparaben, 0,05% EDTA, 4,69% dekstrosa, dan aqua pro-injection. Pengujian dilakukan dengan mengukur kemampuan pembasahan saliva buatan menggunakan indikator besarnya sudut kontak sampel pada permukaan hidrofobik selama 5 menit. Data yang digunakan merupakan rerata nilai sudut kontak pada kedua sisi tetesan di menit ke-0, 0,5, 1, 1,5, 2, dan 5 yang dianalisis menggunakan aplikasi ImageJ. Hasil independent sample t-test menunjukkan sudut kontak saliva buatan mukus lele lebih kecil daripada saliva manusia (p<0,05) pada pengamatan menit ke-0, 0,5, 1, 1,5, 2, dan 5. Kemampuan pembasahan saliva buatan mukus lele memiliki perbedaan yang signifikan dibandingkan dengan saliva manusia, sehingga saliva buatan ini memberikan fungsi lubrikatif dan protektif yang tidak terlalu menyerupai saliva manusia.

RONGGA MULUT SEBAGAI RESERVOIR POTENSIAL UNTUK INFEKSI Pseudomonas aeruginosa Puspita Hajardhini; Heni Susilowati; Heribertus Dedy Kusuma Yulianto
Odonto : Dental Journal Vol 7, No 2 (2020): December 2020
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Introduction : Pseudomonas aeruginosa (P. aeruginosa) is Gram-negative bacteria which is normally inhabiting in environment, however it tends to be an opportunistic pathogen within oral cavity. It utilizes the oral cavity as potential reservoir to infect either lungs or oral cavity itself. Both planktonic and biofilm forms can mediate its infection in oral cavity so that making its difficulties to eradicate since its broad resistance to antibiotics.Method : We review the pathogenesis of oral infection and host defense mechanism to P. aeruginosa as well.Discussion: Several prevention strategies, both chemical and nonchemical, are elaborated to avoid oral bacterial contamination.Conclusion : Prevention strategies in dental aspect to P. aeruginosa is highly needed as its occurrence are susceptible to cross-infection.

The concentration effect of kulim leaf (scorodocarpus borneensis) extract on Streptococcus mutans ATCC 25175 bacterial hydrophobicity and adhesion Utami, Trianna Wahyu; Hapsari, Adhaninggar Ratna; Hanalda, Dhe Rifdania; Nur, Asikin; Yulianto, Heribertus Dedy Kusuma; Purwanti, Nunuk
Majalah Kedokteran Gigi Indonesia Vol 8, No 3 (2022): December
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.80636

With the ability to reduce hydrophobicity and inhibit the adhesion of S. mutans ATCC 25175 bacteria, Kulim leaf extract can be used as an alternative to chlorhexidine mouthwash in caries prevention. The objectives of this study are to determine the effect of Kulim leaf extract on hydrophobicity and adherence of the cariogenic bacteria S. mutans ATCC 25175. The test groups were divided into negative control (DMSO 2%), the treatment group (Kulim leaf extractconcentrations of 1.25%, 2.5%, 5%), and positive control (0.1% chlorhexidine). All of groups were received three replicated tests for hydrophobicity and adherence inhibition of S. mutans ATCC 25175 bacteria. The hydrophobicity test was conducted by providing 3 ml of bacterial suspension of S. mutans ATCC 25175 which had been adjusted to the McFarland 0.5 standard for each group. Furthermore, each test group was vortexed for one minute and left to stand for 15 minutes. Each treatment was tested with a wavelength spectrophotometer of 550 nm before and after the provision of 200 µl of n-hexadecane. The absorbance value on the spectrophotometer was then included in the hydrophobicity formula to determine the hydrophobicity percentage of S. mutans ATCC 25175 against n-hexadecane. To test the bacterial adhesion, the 96 wells microplate was inserted with the kulim leaf extract of each concentration,BHI-B, bacteria according to the McFarland 0.5 standard, and for the positive control and negative control. Afterwards, they were incubated at 37 °C for 24 hours before they were rinsed with distilled water, and stained with 0.1% crystal violet. Then, an optical density reading was performed using a microplate reader with a wavelength of 540 nm. The absorbance value was then included in the formula for percentage of bacterial adhesion inhibition. Post-Hoc LSD testshowed a significant difference in mean difference between the negative control group and the other treatment groups (p<0.05). In addition, it was revealed that there was no significant mean difference between treatment groups, and there was no significant difference between positive control and treatment groups of 2.5% and 5% in the hydrophobicity test. However, there was a significant difference between the positive control and the treatment group of 1.25% in the hydrophobicity test and the treatment group of 1.25%, 2.5%, and 5% in the adherence test. This study concluded that Kulim leaf extract concentration affected hydrophobicity and attachment of S. mutans ATCC 25175 with an effective concentration of 2.5%.

Antibacterial effect of alpha-mangostin from Garcinia mangostana L against oral streptococci and staphylococci biofilms: a systematic review Praptiningsih, Rahmawati Sri; Siswomihardjo, Widowati; Jonarta, Alma Linggar; Yulianto, Heribertus Dedy Kusuma; Syifa, Liftia Layyinatus; Rochmah, Yayun Siti; Ichwan, Solachuddin Jauhari Arief
Padjadjaran Journal of Dentistry Vol 36, No 2 (2024): July 2024
Publisher : Faculty of Dentistry Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol36no2.53957

ABSTRACT Introduction: Interactions among competing bacteria, which inhibit each other's growth to maintain the balance of microorganisms in the biofilm, can influence oral cavity homeostasis. Natural products contain compounds with the potential to combat various diseases, including caries. Alpha-mangostin from Garcinia mangostana L exhibits antibacterial properties against oral streptococci and staphylococci, acting through several mechanisms, including the disruption of peptidoglycan, which ultimately leads to cell brittleness and rupture. This study aims to assess the impact of antibacterial alpha-mangostin on oral streptococci and staphylococci biofilm. Methods: The research methodology employed secondary data collection through a systematic review study. We conducted searches across databases including PubMed, ScienceDirect, and Google Scholar to identify Indonesian and English references encompassing textbooks, research findings, reviews, and internet articles relevant to the topic published between 2014 and 2024. Following a thorough screening process, we selected articles deemed pertinent for inclusion in the review. Subsequently, these articles underwent evaluation for full-text accessibility, language compatibility, and availability of information from the respective journals, resulting in the selection of 20 articles. Result: Analysis was carried out on 20 articles. Ten articles described the effects of alpha-mangosteen's inhibit oral biofilm, 6 articles discussed the effects of alpha-mangosteen's antibacterial activity against Streptococcus mutans and Streptococcus sanguis, 3 article state mechanism alpha-mangostin inhibit membrane enzymes such as F(H+)-ATPase and the phosphoenolpyruvate-sucrose phosphotransferase system on Staphylococcus aureus. Additionally, one article describe inhibition mechanism of aldolase, glyceraldehyde-3-phosphate dehydrogenase, and lactic dehydrogenase on Staphylococcus epidermidis. Conclusion: Alpha-mangostin inhibits and disrupts biofilm defense mechanisms. It possesses antimicrobial properties effective against oral streptococci and staphylococci, including Streptococcus mutans, Streptococcus sanguis, Staphylococcus aureus and Staphylococcus epidermidis. These properties operate through various mechanisms, including enhancing the permeability of bacterial cell walls, ultimately leading to bacterial cell death.

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