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All Journal Jurnal Sain Veteriner Dentika Dental Journal Jurnal Teknosains Majalah Kedokteran Gigi Indonesia ODONTO Dental Journal Dental Journal (Majalah Kedokteran Gigi) Padjadjaran Journal of Dentistry Jurnal Material Kedokteran Gigi Odonto dental journal

Widowati Siswomihardjo

Universitas Gadjah Mada

Author-ID : 4323450

Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Dentistry Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Public Health Veterinary

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JUMLAH OSTEOBLAS PADA PROSES PENYEMBUHAN TULANG PASCA IMPLANTASI HIDROKSIAPATIT SINTESIS DARI KALSIT: NUMBER OF OSTEOBLAST IN BONE HEALING PROCESS AFTER IMPLANTATION OF SYNTHETIC HYDROXYAPATITE FROM CALCITE Hengky Bowo Ardhiyanto; Widowati Siswomihardjo; Tetiana Haniastuti
Dentika: Dental Journal Vol. 17 No. 2 (2012): Dentika Dental Journal
Publisher : TALENTA

Tulang memiliki respons penyembuhan secara alami, namun defek yang besar seringkali menyebabkan tulang tidak dapatberegenerasi dengan baik. Hidroksiapatit (HA) adalah material pengganti tulang yang sudah sering digunakan sebagaibone graft. Material ini dapat disintesis dari berbagai sumber, antara lain dari batuan kalsit. Tujuan penelitian ini adalahuntuk mengetahui jumlah sel osteoblas pada proses penyembuhan tulang setelah diimplantasi dengan hidrosiapatit sintesisdari kalsit sebagai material bone graft. Limapuluh delapan ekor tikus Sprague dawley dibagi menjadi 3 kelompok.Kelompok pertama adalah tikus yang diimplantasi dengan HA kalsit, kelompok kedua adalah tikus yang diimplantasidengan HA 200, dan kelompok ketiga tidak mendapat perlakuan (kontrol). Defek dibuat pada kondilus tulang femurdengan ukuran yang sama (diameter 3mm dan kedalaman 3mm). Enam ekor tikus pada masing-masing kelompokdikorbankan pada hari ke-14, 28 dan 56. Spesimen kemudian diproses secara histologis dan diwarnai denganhematoksilin-eosin. Hasil penelitian menunjukkan jumlah sel osteoblas pada tikus yang diimplantasi dengan HA 200lebih tinggi jika dibandingkan dengan HA sintesis kalsit. Jumlah sel osteoblas pada tikus yang diimplantasi dengan HAsintesis kalsit lebih tinggi bila dibandingkan dengan kelompok kontrol, secara statistik tidak terdapat perbedaan yangbermakna jumlah osteoblas antar kelompok (p>0,05). Sebagai kesimpulan, implantasi HA sintesis dari kalsit sebagaibone graft dapat meningkatkan jumlah sel osteoblas pada proses penyembuhan tulang, namun peningkatannya tidakbermakna.

EFFECT OF IMMERSION TIME TO MECHANICAL STRENGTH OF POLYVINYL ALCOHOLâ€“ HYDROXY APATITE COMPOSITE AS BIODEGRADABLE BONE PLATE : PENGARUH WAKTU PERENDAMAN TERHADAP KEKUATAN MEKANIS KOMPOSIT POLIVINIL ALKOHOLâ€“HIDROKSI APATIT SEBAGAI FIKSASI FRAKTUR TULANG Mochammad Taha Maâ€™ruf; Widowati Siswomihardjo; Marsetyawan Heparis Nur Ekanda Soesatyo; Alva Edy Tontowi
Dentika: Dental Journal Vol. 17 No. 4 (2013): Dentika Dental Journal
Publisher : TALENTA

Polymer based material such as Poly-L-Lactic-Acid (PLLA) have been used for titanium substitution. However, the last research indicates that it was not an ideal material as bone fracture fixation device. It is a challenge to develop other materials as biodegradable bone plate for titanium substitution, such as polyvinyl alcohol (PVA). In this study, polyvinyl alcohol has composited with Hydroxy apatite (HA) and reinforced with catgut fiber. The aim of this study was to know the effect of immersion time to mechanical strength of PVAâ€“ HA composite with catgut reinforced as biodegradable bone plate after biodegradation test for 30 and 60 days. Specimens were prepared for tensile and bending strength tests, immersed in phosphate buffered saline (PBS) solution with pH 7.4 at 370C. Specimens were weighed before and after biodegradation test, tensile and bending test were done after removed for 30 and 60 days. The result showed that PVAHA composite reinforced with catgut fiber has stable mechanical strength after 30 and 60 days biodegradation process. The mechanical strength decreased in 30 and 60 days in all intervention groups, but it still has adequate mechanical strength as biodegradable bone plate. In conclusion, PVA-HA composite reinforced with catgut fiber can be developed as biodegradable bone plate replace titanium and other commercial bone plate.

EFEK KOMPOSISI PARAFIN DAN MALAM LEBAH TERHADAP EKSPANSI TERMAL LINIER MALAM MODEL: EFFECT PARAFFIN AND BEESWAX RATIO TO LINEAR EXPANSION OF MODELLING WAX Fitri Isnaini; Dyah Irnawati; Widowati Siswomihardjo
Dentika: Dental Journal Vol. 14 No. 1 (2009): Dentika Dental Journal
Publisher : TALENTA

Modelling wax is a pattern wax for complete denture fabrication. It consists of various waxes and other components. Thecomposition influenced the physical properties of modelling wax. The objective of this study was to examine the effect ofparaffn to beeswax ratio on the linear thermal expansion (LTE) of modelling wax. The modelling waxes consist of paraffin (Pertamina, Kalimantan), beeswax (SEA, Yogyakarta), carnauba wax (PT Bratachem, Indonesia), and dammar(Kalimantan). Five compositions of waxes were made with different paraffin to beeswax ratio (paraffin: beeswax: carnauba wax: dammar in % weight): K1 = 70:20:4:6, K2 = 72.5:17,5:4:6, K3 = 75:15:4:6, K4 = 77.5:12.5:4:6, and K5 =80:10:4:6. Six specimens (305 x 22.2 x 14.3 mm) were made of each composition. The specimens were immersed inbathing water at 25Â°C and 40Â°C for 20 minutes, and the specimen length was measured by travelling microscope aftereach immersion. The percentage of the LTE was measured. Data were analyzed by one-way Anova and LSD0.05. Theresults showed that the LTE of modelling waxes was between 0.27 + 0.02 % (70:20 ratio) and 0.73 + 0.06 % (80:10ratio). The Paraffin to beeswax ratio influenced the LTE of modelling waxes and showed the significant differencesbetween all compositions (p<0.5). As conclusion, the paraffin to beeswax ratio influenced the linear thermal expansion ofmodelling wax.

EVALUATION ON RESIDUAL MONOMER OF HDDMA MATRIX SYSTEM ON FIDER REINFORCED CONDOSITES (FRC) : EVALUASI MONOMER RESIDU DARI SISTEM MATRIKS HDDMA PADA FIDER REINFORCED CONDOSITES(FRC) Siti Sunarintyas; Widowati Siswomihardjo; Dyah Irnawati; Jukka Pekka Matinlinna
Dentika: Dental Journal Vol. 18 No. 2 (2014): Dentika Dental Journal
Publisher : TALENTA

Matrix system used bis-GMA was reported hazardously. An alternative monomer such as 1,6-hexanediol dimethacrylate(HDDMA) was under research. The aim of this study was to evaluate residual monomer content of HDDMA basedmatrix compositions on FRCs (fiber-reinforced composites). Three monomers based on bis-GMA (Sigma-Aldrich,USA),methylmethacrylate (MMA, ProSciTech, Australia), HDDMA (Esstech, USA) were used and also camphorquinone (CQ,Esstech, USA), N,N-cyanoethyl methylaniline (CEMA, Esstech, USA), E-glass fibers (StickTech, Finland). The matrixratios (weight %) were 78.4% bis-GMA+19.6% MMA+1.0% CQ+1.0% CEMA (control-group), 78.4% HDDMA+19.6% MMA+1.0% CQ+1.0% CEMA (EXP-1group), and 49.0% HDDMA+49.0% MMA+1.0% CQ+1.0% CEMA(EXP-2group). Samples with fibers embedded in matrix were light-cured then powdered. Powder of 150mg was dilutedin acetonitrile to 10ml. The solution was filtered then injected into HPLC (20ÂµL). Residual monomer content wasevaluated by mobile phase of acetonitrile and water of 7:3, flow rate 1mL/minute. The size of column was C185Âµ,125mm length, 4mm diameter. UV detection used 275nm. Data were analyzed by ANOVA. The result showed theaverage of residual monomer content (in %) was: 0.02125Â±0.00018 (control-group), 0.01660Â±0.00016 (EXP-1group),0.01676Â±0.00033 (EXP-2group). The ANOVA showed significant difference of monomer content among the groups(p<0.05). The LSD showed significant difference between EXP-1 and control-groups; also between EXP-2 and controlgroups (p<0.05). There was no significant difference between EXP-1 and EXP-2 groups (p>0.05). In conclusion, a resinmatrix system based on HDDMAâ€“MMA (EXP-1 and EXP-2 groups) revealed significant difference of residualmonomer content to bis-GMAâ€“MMA (control-group) system. The HDDMA-MMA matrix system had less residualmonomer content than bis-GMA-MMA.

Pengaruh Lama Perendaman Fiber Reinforced Composite dengan Fiber Sisal (Agave sisalana) Terkalissai dalam Saliva Buatan Terhadap Perubahan Dimensi Wibowo, Diva Agrita Dentisia; Widjijono, Widjijono; Siswomihardjo, Widowati
Jurnal Material Kedokteran Gigi Vol 7 No 1 (2018): JMKG Vol 7 No 1 Maret 2018
Publisher : Ikatan Peminat Ilmu Material dan Alat Kedokteran Gigi (IPAMAGI)

Fiber reinforced composite is a material composed of matrix and reinforced by fiber. Fiber sisal is a natural fiber an alternatives synthetic fiber developed as dental material. Resin composite matrix is a hydrophilic substance that cause dimensional change. Alkalization of sisal fiber reduces the ability of hydrophilic sites which affects the dimensional changes. The aim of this study was to determine how different period of immersion affect dimensional change in samples. The samples used in this research were FRC made from flowable resin composite (Master Flow, Brazil), and alkalized sisal fiber. The samples were shaped into cuboid measuring 25x2x2 mm. Fiber was placed in the middle of sample with continuous unidirectional direction and then irradiated for 20 seconds using LCU. A total of 16 samples were divided to 4 groups, each group with the different period of immersion 0, 7, 14 and 21 days respectively. Dimensional changes were measured by the final dimension minus the initial dimension. Dimensional change was measured by the length, width, and height using digital sliding caliper with 0.001 mm nonius. The samples were immersed in the artificial saliva with pH 7. The data was analyzed using one way Anova and followed by LSD0.05. The result shows the mean value and standard deviation of dimensional changes with period of immersion at 0, 7, 14 and 21 days were 0.264Â±0.085, 2.856Â±0.089, 4.417Â±0.115 and 4.294Â±0.087 mm3. The F value obtained from one way Anova was 1.640Â±103 and the significance was 0.001 (p<0.05), indicated a significance difference. LSD presented a significance difference of mean to all treat,ent except for 14th and 21th day. This study concluded that various period of immersion increase effect to the dimensional changes of FRC with alkalized fiber sisal.

Effect of immersion time in mouthwash on the flexural strength of polyethylene fiber-reinforced composite Akmal, Syazwani; Siswomihardjo, Widowati; Sunarintyas, Siti
Majalah Kedokteran Gigi Indonesia Vol 6, No 1 (2020): April
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.40194

Fiber-reinforced composite (FRC) fixed dentures are exposed to various oral environments. One of the ways in maintaining good oral condition is by using mouthwash. Questions have been araised about the safety of prolongeduse of mouthwash towards FRC. The aim of this study was to evaluate the effect of immersion time in mouthwash on the flexural strength of polyethylene FRC. The specimens used were polyethylene FRC (Construct, KerrLab, USA) and flowable composite resin (Master Flow Biodinamica, Brazil). Block shaped specimens (2x2x25 mm) was light cured (n=12). Specimens were divided into threek groups of immersion: Group 1 (without immersion); Group 2 (24 hour immersion); Group 3 (48 hour immersion). The mouthwash used was Listerine Multi-Protect (Johnson & Johnson, Indonesia) (21.6% alcohol content). Flexure strength was measured by Universal Testing Machine. The results showed the average strength value (MPa) for Group 1 was 91.318 Â± 12.466, Group 2 was 62.253 Â± 8.027, and Group 3 was55.033 Â± 3.373. Statistical analysis (ANOVA) showed that immersion time in mouthwash influenced the flexural strength of polyethylene FRC (p<0.05). LSD0.05 showed there were significant differences of flexural strength between Group1-Group 2, Group 1-Group 3 but not for Group 2-Group 3. In conclusion, the flexural strength of polyethylene FRC were decreased by the immersion time in mouthwash.

Campuran kitosan dengan resin akrilik sebagai bahan gigi tiruan penghambat Candida albicans Titik Ismiyati; Widowati Siswomihardjo; Marsetyawan Heparis Nur Ekandaru Soesatyo; R. Rochmadi
Majalah Kedokteran Gigi Indonesia Vol 3, No 3 (2017): December
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

The mixture of acrylic resin and chitosan as denture material to inhibit Candida albicans. The inhibition of Candida albicans in denture resin has an important role to prevent the development of denture stomatitis. Chitosan is a natural polymer compound derived from shrimp waste which can function as an antifungal Acrylik resin cannot be mixed with chitosan. To obtain a homogeneous mixture, the mixture was added a coupling agen acrylic acid and acetone. The research objective was to study the mixture of acrylic resin and chitosan with solvent acrylic acid and acetone as a denture that can inhibit the growth of Candida albicans. Methods: The samples used discs in 10 mm diameter and 2 mm thickness, made from heat cured acrylic resin mixed with chitosan dissolved in acrylat acid and acetone. They were divided into 4 groups. Group 1 was acrylic resin without chitosan as a control, group 2, 3 and 4 were the mixture of acrylic resin and 5 ml chitosan in 0.5%, 1%, and 2% concentration respectively. The fourier transform irfrared spectroscopy (FTIR) and the digital optical microscope were used to synthesize and analyze. The Kruskal Wallis was used to analyze the data. The results showed that the mixture of acrylic resin with chitosan significantly inhibited the growth of Candida albicans. Conclusion: a mixture of acrylic resins and chitosan can be fungistatic, so it can be developed as an antifungal denture material. ABSTRAKPenghambatan Candida albicans pada gigi tiruan resin akrilik dapat memainkan peran penting dalam mencegah perkembangan denture stomatitis. Kitosan adalah senyawa polimer alam yang berasal dari limbah udang yang dapat berfungsi sebagai antijamur. Resin akrilik tidak dapat bercampur dengan kitosan. Untuk mendapatkan campuran yang homogen, campuran tersebut ditambah coupling agent asam akrilat dan aseton. Penelitian ini bertujuan untuk mengkaji campuran resin akrilik dan kitosan dan asam akrilat pelarut aseton sebagai bahan gigi tiruan penghambat Candida albicans. Spesimen penelitian berbentuk cakram berdiameter 10 mm dan tebal 2 mm dibuat dari resin akrilik kuring panas (QC 20) dicampur dengan kitosan dari cangkang udang yang ditambahkan asam akrilat dalam pelarut aseton. Spesimen dibagi menjadi 4 kelompok. Kelompok 1 terdiri dari resin akrilik tanpa kitosan sebagai kontrol, sedangkan kelompok 2, 3, dan 4 terdiri dari campuran resin akrilik dengan kitosan 5 ml pada konsentrasi 0,5%, 1%, dan 2%, secara berurutan. Hasil campuran resin akrilik dengan kitosan dianalisis dengan menggunakan Fourier Transform Infrared spectroscopy (FTIR), dan digital mikroskop optik. Efek antijamur diuji dengan menggunakan metode dilusi. Data yang didapat dianalisis statistik dengan Kruskal Wallis. Hasil penelitian menunjukkan bahwa campuran resin akrilik dengan kitosan signifikan menghambat pertumbuhan Candida albicans. Berdasarkan hasil yang diperoleh, dapat disimpulkan bahwa campuran resin akrilik dan kitosan dapat bersifat fungistatik, sehingga dapat dikembangkan sebagai bahan gigi tiruan antijamur.

Effect of immersion time in mouthwash on the flexural strength of polyethylene fiber-reinforced composite Syazwani Akmal; Widowati Siswomihardjo; Siti Sunarintyas
Majalah Kedokteran Gigi Indonesia Vol 6, No 1 (2020): April
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.40194

Fiber-reinforced composite (FRC) fixed dentures are exposed to various oral environments. One of the ways in maintaining good oral condition is by using mouthwash. Questions have been araised about the safety of prolongeduse of mouthwash towards FRC. The aim of this study was to evaluate the effect of immersion time in mouthwash on the flexural strength of polyethylene FRC. The specimens used were polyethylene FRC (Construct, KerrLab, USA) and flowable composite resin (Master Flow Biodinamica, Brazil). Block shaped specimens (2x2x25 mm) was light cured (n=12). Specimens were divided into threek groups of immersion: Group 1 (without immersion); Group 2 (24 hour immersion); Group 3 (48 hour immersion). The mouthwash used was Listerine Multi-Protect (Johnson & Johnson, Indonesia) (21.6% alcohol content). Flexure strength was measured by Universal Testing Machine. The results showed the average strength value (MPa) for Group 1 was 91.318 ± 12.466, Group 2 was 62.253 ± 8.027, and Group 3 was55.033 ± 3.373. Statistical analysis (ANOVA) showed that immersion time in mouthwash influenced the flexural strength of polyethylene FRC (p<0.05). LSD0.05 showed there were significant differences of flexural strength between Group1-Group 2, Group 1-Group 3 but not for Group 2-Group 3. In conclusion, the flexural strength of polyethylene FRC were decreased by the immersion time in mouthwash.

UTILIZATION OF JKN DENTAL PROSTHETIC SERVICES AT FIRST LEVEL DENTAL HEALTH FACILITIES IN BALI Gusti Ayu Yohanna Lily; Widowati Siswomihardjo; Julita Hendrartini; Haryo Mustiko Dipoyono; Dewa Made Wedagama
ODONTO : Dental Journal Vol 8, No 2 (2021): December 2021
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.8.2.126-130

ABSTRACTBackground: The utilization of dental prosthesis services in Indonesia is rare, compared to other dental and oral health services. The use of prosthesis services in Bali is still relatively low compared to other parts of Western Indonesia. Based on data from BPJS Kesehatan Denpasar Branch Office, the demand for dental prostheses services by participants in the three working areas of BPJS Kesehatan Denpasar Branch Office is still low. Method: This research was conducted using quantitative research. The population were all participants providers of FKTP BPJS Kesehatan Badung Regency, and samples were taken through purposive sampling. Quantitative data were analyzed using binary logistic regression.Result: The results that the predisposing factors for JKN program participants (income, knowledge of dental prostheses, knowledge of JKN dental prosthesis services, perceptions of service quality, and perceptions of cost); enabling factor (access), and the need factor (the perception of pain when losing a tooth) contributions have a significant positive effect on the utilization rate of JKN dental prostheses. The predisposing factors (age and education) and enabling factor (social support) do not have a significant effect on the utilization rate of JKN dental prostheses. Conclusion: The results that the predisposing factors for JKN program participants contributions have a significant positive effect on the utilization rate of JKN dental prostheses.

PENGARUH KHLORHEKSIDIN DIGLUKONAT 2% DAN GLISERIN SEBAGAI BAHAN PENCAMPUR KALSIUM HIDROKSIDA TERHADAP SISA KALSIUM HIDROKSIDA PADA SEPERTIGA APIKAL DINDING SALURAN AKAR GIGI Emmawati Prawitasari, Diatri Nari Ratih, dan Widowati Siswomihardjo
Jurnal Teknosains Vol 3, No 1 (2013): December
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/teknosains.6127

The most widely used intracanal medicament is calcium hydroxide (Ca(OH2). However, the residue ofCa(OH)2 in the root canal must be removed prior to obturation. The residue of Ca(OH)2 in the root canalwalls would result in apical leakage. This is due to the unstable dimension of Ca(OH)2, reduced flowand working time of the sealers, and decreased adhesion of sealers and gutta percha to root canal walls.This study aimed to determine the effect of Ca(OH)2 vehicles and agitation irrigation techniques on theresidue of Ca(OH)2 in the apical third of the root canal walls.Twelve extracted mandibular first premolars were used in this study. The crowns of the teethwere removed at the apical part of cemento enamel junction with a length of 14 mm from the apical.The canals were prepared with a Step Back technique to obtain Master Apical File ( MAF ) # 40. Thespecimens were randomly devided into groups I, the pastes of Ca(OH)2+chlorhexidine digluconate 2%.In groups II, the pastes of Ca (OH)2+ glycerin were applied. Each specimen of the study was sectionedin the sagittal direction, then was photographed under a stereo microscope at 120x magnification. Thepercentages of Ca(OH)2 residue were calculated using UTHSCSA image tool 3 software. Data wereanalyzed using T-test at 95% level of significance.The results revealed that the group of Ca(OH)2+ chlorhexidine digluconate 2% paste showed thelowest number of residual Ca(OH)2.The conclusion of this study was that the vehicles affected the totalresidue of Ca(OH)2.

Co-Authors Afra Hatim Agustina Dwi Wijayanti Akmal, Syazwani Alma Linggar Jonarta Alva Edy Tontowi Arifianto, Dinar Dewa Made Wedagama Dian Handayani Dinar Arifianto Dyah Anindya Widyasrini Dyah Irnawati Fadriyanti, Okmes Fitri Isnaini Gusti Ayu Yohanna Lily Harsini Haryo Mustiko Dipoyono Hasna Syifa Yuniva Hengky Bowo Ardhiyanto Heri Wijayanto Heribertus Dedy Kusuma Yulianto Hilda Mutia Hanum Ichwan, Solachuddin Jauhari Arief Jukka Pekka Matinlinna Julita Hendrartini Karina Larasati Liftia Layyinatus Syifa Marsetyawan H.N.E. Soesatyo Marsetyawan Heparis Nur Ekanda Soesatyo Mochammad Taha Ma’ruf Nahar Taufiq Nasution, Ismet Danial Prisca Dhyaning Dentiana R. Rochmadi Rahmawati Sri Praptiningsih Raymund Octavius Kusuma Buwana Rochmadi Rochmadi Setyo Budhi Setyo Budhi Siti Sunarintyas Soesatyo, Marsetyawan Heparis Nur Ekandaru Sri Kavi Subramaniam Syazwani Akmal Titik Ismiyati Verani, Dhea Maurilla W. Widjijono Wibowo, Diva Agrita Dentisia Yasnawi, Mohammad Imanuddin Yayun Siti Rochmah

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