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UJI ANTIBAKTERI EKSTRAK ETANOL DAUN TAHONGAI (Kleinhovia hospita Linn) TERHADAP PERTUMBUHAN BAKTERI Actinobacillus actinomycetemcomitans IN-VITRO Cynthia Clarissa; Masyhudi Amir; Verry Asfirizal
Jurnal Kedokteran Mulawarman Vol 7, No 3 (2020): Jurnal Kedokteran Mulawarman
Publisher : Fakultas Kedokteran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30872/j.ked.mulawarman.v7i3.5020

Abstract

Tahongai (Kleinhovia hospita Linn) merupakan tanaman herbal yang banyak dijadikan obat tradisional oleh masyarakat suku Dayak. Tahongai memiliki berbagai khasiat yang dapat digunakan untuk menunjang kesehatan tubuh. Didalam tanaman ini juga terdapat senyawa aktif yang dapat berfungsi sebagai zat antibakteri. Terutama pada bagian daun Tahongai, memiliki kandungan senyawa aktif seperti flavonoid, alkaloid, saponin, dan terpenoid yang dapat berfungsi sebagai senyawa antibakteri. Penelitian ini bertujuan untuk mengetahui efek antibakteri dari ekstrak daun tanaman Tahongai terhadap pertumbuhan bakteri Actinobacillus actinomycetemcomitans secara In-vitro. Desain penelitian ini adalah post test only control group design dengan menggunakan uji Disc Diffusion (Kirby-Bauer). Penelitian ini menggunakan bakteri A. actinomycetemcomitans yang diberi perlakuan ekstrak etanol daun Tahongai (K. hospita Linn) dengan konsentrasi 5%, 10%, 15%, dan 20%. Kemudian dilakukan pengulangan sebanyak lima kali. Hasil penelitian menunjukkan bahwa ekstrak etanol daun Tahongai (K. hospita Linn) tidak mampu menghambat pertumbuhan bakteri A. actinomycetemcomitans pada konsentrasi 5%, 10%, 15%, dan 20%. 
EFEKTIVITAS EKSTRAK ETANOL DAUN BELUNTAS PLUCHEA INDICA (L.) LESS TERHADAP BAKTERI SALIVA SECARA IN VITRO Annisa Fairus Syafira; Masyhudi Masyhudi; Sinar Yani
ODONTO : Dental Journal Vol 6, No 2 (2019): December 2019
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.6.2.68-75

Abstract

Background: Pluchea indica (L.) Less leaf contains antibacteria substances that can inhibit the growth of bacteria that cause dental and oral diseases. Previous research shows that Pluchea indica (L.) Less leaf extract can inhibit the growth of Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa bacteria. This research aimed to find out effectiveness of Pluchea indica (L.) Less leaves to decrease in the number of salivary bacteria colony.Method: This research used a sample of saliva obtained from a person who has dental caries. The saliva had been treated with Pluchea indica (L.) Less leaves ethanol extract with concentration 2,5%, 3,5%, 4,5%, 5,5%, and 6,5%. Bacterial colonies were calculated using a colony counter and no identification was performed.Result: The mean of bacteria colony number in Plate Count Agar media without Pluchea indica (L.) Less leaves ethanol extract is 142,67. After being given Pluchea indica (L.) Less leaves ethanol extract with concentration of 2,5%, 3,5%, and 4,5%, the mean of bacteria colony number sequentially are 15,67, 10,67, 4, and at a concentration of 5.5% and 6.5% no more bacterial colonies were found.Conclusion: Pluchea indica (L.) Less leaves ethanol extract effective to inhibit and eliminate oral bacteria.
The Antibacterial Activity of Leaves Ethanol Extract, Stem Bark, and Latex of Angsana (Pterocarpus indicus Willd.) towards Bacterial Growth Streptococcus mutans as in vitro Dera Armedita; Verry Asfrizal; Masyhudi Amir
ODONTO : Dental Journal Vol 5, No 1 (2018): July 2018
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.5.1.1-8

Abstract

Background: Indonesia has many types of plants, which have medicinal properties and are used to cure various diseases. One of plant that has medicinal properties is the Angsana plant (Pterocarpus indicus Willd.) which traditionally can cure dental and oral diseases.Method: This research used experimental laboratory with disc diffusion methods. Angsana (Pterocarpus indicus Willd.) was taken from as city ornamental tree in Samarinda city. The bacteria which used were Streptococcus mutans. The samples consisted of 11 treatment groups is leaves ethanol extract, stem bark, and Angsana latex respectively with concentration 25%, 50%, 75%, positive control group (chlorhexidine 0,2%), and negative control group (sterile aquades). Data analysis using Shapiro-Wilk test and One-Way Annova test.Result: The results showed that leaves ethanol extract, stem bark, and Angsana latex (Pterocarpus indicus Willd.). All concentrations have antibacterial activity against the growth of Streptococcus mutans. The highest and the lowest concentrations that can inhibit the growth of Streptococcus mutans are 50% ethanol stem bark extract and 25% leaves ethanol extract.Conclusion: The conclusion of this study proves that the leaves, stem bark ethanol extract, and Angsana latex (Pterocarpus indicus Willd.) Have antibacterial activity against the growth of Streptococcus mutans.
Antibacterial activity of bangle rhizome essential oil (zingiber montanum) against streptococcus mutans Devina RS. Pardosi; Cicih B. Purnamasari; Swandari Paramita; Lilies A. Astuti; Masyhudi; Enos T. Arung
Journal of Dentomaxillofacial Science Vol. 7 No. 1 (2022): (Available online: 1 April 2022)
Publisher : DiscoverSys Inc.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15562/jdmfs.v7i1.1398

Abstract

Objective: This research was conducted to determine the potential of bangle rhizome essential oil (zingiber montanum) as an antibacterial against the growth of streptococcus mutans. Material and Methods: Tests are carried out using the diffusion method for the well’s technique. In this study, eugenol was used as a positive control. Essential oil was diluted with acetone and obtained essential oil of bangle rhizome with a concentration of 3.12%, 6.25%, 12.5%, 25%, and 50%. Results: That bangle rhizome essential oil succeeded in inhibiting the growth of streptococcus mutans with the minimum inhibitory concentration 3.12% and with the optimal inhibitory concentration 50%. Conclusion: It is concluded from this research that essential oil from bangle rhizome is a strong, new natural antibacterial agent for streptococcus mutans. There was a slight difference of antibacterial effectiveness between eugenol, as positive control, and essential oil from bangle rhizome on streptococcus mutans bacterial growth observed from their inhibition zones.
Risk prevalence of the temporomandibular disorder in Students Faculty of Medicine, Mulawarman University, Class of 2018-2020 Imran Irsal; Nanda Pratiwi; Masyhudi; Elliana Martalina; Portuna P. Kambaya; Alhawaris; Aziz Mohpul; Acing H. Mude
Journal of Dentomaxillofacial Science Article In Press
Publisher : DiscoverSys Inc.

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Objective: This study described the risk prevalence of Temporomandibular Disorder in students Faculty of Medicine, Mulawarman University, Class of 2018-2020. Material and Methods: This research is a descriptive study using a survey method. Results: The sample consisted of 504 participants, 136 of them were men (27%) and 368 of them were women (73%), with an average age of 19.36 years old. Conclusion: It is concluded from this research that women had a high risk of experiencing temporomandibular disorder by 65.9%. The age that has a high risk of experiencing temporomandibular disorder is 20 years old with a prevalence of 29.0%. Class 2019 students who have a high risk of experiencing TMD are 32.5%.
The Antibacterial Activity of Leaves Ethanol Extract, Stem Bark, and Latex of Angsana (Pterocarpus indicus Willd.) towards Bacterial Growth Streptococcus mutans as in vitro Dera Armedita; Verry Asfrizal; Masyhudi Amir
Odonto : Dental Journal Vol 5, No 1 (2018): July 2018
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (470.697 KB) | DOI: 10.30659/odj.5.1.1-8

Abstract

Background: Indonesia has many types of plants, which have medicinal properties and are used to cure various diseases. One of plant that has medicinal properties is the Angsana plant (Pterocarpus indicus Willd.) which traditionally can cure dental and oral diseases.Method: This research used experimental laboratory with disc diffusion methods. Angsana (Pterocarpus indicus Willd.) was taken from as city ornamental tree in Samarinda city. The bacteria which used were Streptococcus mutans. The samples consisted of 11 treatment groups is leaves ethanol extract, stem bark, and Angsana latex respectively with concentration 25%, 50%, 75%, positive control group (chlorhexidine 0,2%), and negative control group (sterile aquades). Data analysis using Shapiro-Wilk test and One-Way Annova test.Result: The results showed that leaves ethanol extract, stem bark, and Angsana latex (Pterocarpus indicus Willd.). All concentrations have antibacterial activity against the growth of Streptococcus mutans. The highest and the lowest concentrations that can inhibit the growth of Streptococcus mutans are 50% ethanol stem bark extract and 25% leaves ethanol extract.Conclusion: The conclusion of this study proves that the leaves, stem bark ethanol extract, and Angsana latex (Pterocarpus indicus Willd.) Have antibacterial activity against the growth of Streptococcus mutans.
EFEKTIVITAS EKSTRAK ETANOL DAUN BELUNTAS PLUCHEA INDICA (L.) LESS TERHADAP BAKTERI SALIVA SECARA IN VITRO Annisa Fairus Syafira; Masyhudi Masyhudi; Sinar Yani
Odonto : Dental Journal Vol 6, No 2 (2019): December 2019
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (662.098 KB) | DOI: 10.30659/odj.6.2.68-75

Abstract

Background: Pluchea indica (L.) Less leaf contains antibacteria substances that can inhibit the growth of bacteria that cause dental and oral diseases. Previous research shows that Pluchea indica (L.) Less leaf extract can inhibit the growth of Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa bacteria. This research aimed to find out effectiveness of Pluchea indica (L.) Less leaves to decrease in the number of salivary bacteria colony.Method: This research used a sample of saliva obtained from a person who has dental caries. The saliva had been treated with Pluchea indica (L.) Less leaves ethanol extract with concentration 2,5%, 3,5%, 4,5%, 5,5%, and 6,5%. Bacterial colonies were calculated using a colony counter and no identification was performed.Result: The mean of bacteria colony number in Plate Count Agar media without Pluchea indica (L.) Less leaves ethanol extract is 142,67. After being given Pluchea indica (L.) Less leaves ethanol extract with concentration of 2,5%, 3,5%, and 4,5%, the mean of bacteria colony number sequentially are 15,67, 10,67, 4, and at a concentration of 5.5% and 6.5% no more bacterial colonies were found.Conclusion: Pluchea indica (L.) Less leaves ethanol extract effective to inhibit and eliminate oral bacteria.
Uji Efek Antiinflamasi Ekstrak Etanol Mitragyna (Mitragyna Speciosa) Pada Mencit (Mus Musculus) Dengan Metode Induksi Xylene Pada Telinga Mencit Wilman Rante Marampa; Sjarif Ismail; Masyhudi Amir
Syntax Literate Jurnal Ilmiah Indonesia
Publisher : Syntax Corporation

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (86.546 KB) | DOI: 10.36418/syntax-literate.v7i6.7279

Abstract

Latar Belakang: Daun sapat (Mitragyna speciosa) diketahui mengandung zat aktif flavanoid, dan alkaloid, serta memiliki efek antiinflamasi dengan metode edema kaki yang diinduksi karagenan pada tikus. Namun, efek antiinflamasi ekstrak etanol daun Mitragyna speciosa (MS) dengan metode induksi xylene pada telinga mencit belum pernah dilaporkan. Tujuan: Mengetahui efek antiinflamasi ekstrak Mitragyna speciosa dengan metode induksi xylenepada telinga mencit Metode: Mencit jantan dibagi ke dalam 5 kelompok (n=5): kontrol negatif (CMC 0,5%), kontrol positif (natrium diklofenak 0,013 mg/gr BB), dosis I (ekstrak MS 0,5 mg/gr BB), dosis II (ekstrak MS 1 mg/gr BB), dosis III (ekstrak MS 2 mg/gr BB) diberikan secara oral sebelum induksi xylene pada telinga mencit. Ketebalan telinga diukur dengan digimatic calliper pada menit ke-30,60,90, dan 120 setelah diinduksi xylene. Efek antiinflamasi dinyatakan dengan nilai AUC (Area Under Curve) edema dan disajikan dalam mean ± SD. Uji statistik dengan ANOVA berbeda bermakna jika p<0,05 Hasil: Nilai AUC edema masing-masing kelompok yaitu kontrol negatif (77±11,6), kontrol positif (31,72 ± 6,67), dosis I (46,37 ± 10,04), dosis II (32,33± 11,24), dan dosis III (26,69 ± 6,57). Uji ANOVA (p=0,01), dosis I, dosis II, dan dosis III berbeda bermakna (p<0,05) dengan kontrol negatif Kesimpulan: Ekstrak MS mempunyai efek anti-inflamasi pada telinga mencit yang diinduksi xylene
Perawatan Gigi yang dilakukan Mahasiswa Pengguna Ortodonti Cekat di Universitas Mulawarman pada Masa Pandemi Covid-19: Dental Care Performed by Students Using Fixed Orthodontics at Mulawarman University during the COVID-19 Pandemic Ananda Putri Apriliyana; Masyhudi Masyhudi; Saiful Rokhim
Jurnal Sains dan Kesehatan Vol. 5 No. 3 (2023): J. Sains Kes.
Publisher : Fakultas Farmasi, Universitas Mulawarman, Samarinda, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25026/jsk.v5i3.707

Abstract

Fixed orthodontic treatment requires the ability to maintain dental and oral hygiene which is more difficult and takes a long time, continuously, and some orthodontic procedures produce droplets and aerosols, so many orthodontic patients have to postpone their treatment schedule to minimize the risk of spreading COVID-19. The purpose of this study was to find out how dental care was carried out by students using fixed orthodontics at Mulawarman University during the COVID-19 pandemic. This research is a descriptive observational research type and the sample was selected by simple random sampling technique. The sample consisted of 96 respondents from 13 faculties at Mulawarman University. The data obtained are primary data from the questionnaire. The results showed that more respondents brushed their teeth twice a day (59.38%), consumed sugar (48.96%), visited the dentist (44.79%) and were willing to attend dentist appointments during the COVID-19 pandemic. (50%), used a soft toothbrush (38.54%), and did not use dental hygiene aids (56.25%). It is hoped that the results of this study can be useful for further research related to oral hygiene for fixed orthodontic users so that proper oral hygiene maintenance during orthodontic treatment can be carried out. Keywords: fixed orthodontics, dental care, oral hygiene, pandemic, droplet, aerosol Abstrak Perawatan ortodonti cekat membutuhkan kemampuan pemeliharaan kebersihan gigi dan mulut yang lebih sulit dan memakan waktu yang lama, berkelanjutan, serta beberapa prosedur ortodonti menghasilkan droplet dan aerosol, sehingga banyak pasien pengguna ortodonti yang harus menunda jadwal perawatan mereka untuk meminimalisir risiko penyebaran COVID-19. Tujuan penelitian ini adalah untuk mengetahui cara perawatan gigi yang dilakukan mahasiswa pengguna ortodonti cekat di Universitas Mulawarman pada masa pandemi COVID-19. Penelitian ini merupakan jenis penelitian observasional deskriptif dan sampel dipilih dengan teknik simple random sampling. Sampel terdiri dari 96 responden yang berasal dari 13 Fakultas di Universitas Mulawarman. Data yang diperoleh yakni data primer dari kuesioner. Hasil penelitian menunjukkan responden lebih banyak menyikat gigi dua kali sehari (59,38%), mengonsumsi gula (48,96%), mengunjungi dokter gigi (44,79%) dan bersedia menghadiri janji temu dengan dokter gigi selama masa pandemi COVID-19 (50%), menggunakan tipe sikat gigi soft (38,54%), dan tidak menggunakan alat bantu kebersihan gigi (56,25%). Diharapkan hasil penelitian ini dapat bermanfaat untuk penelitian selanjutnya terkait kebersihan mulut bagi pengguna ortodonti cekat sehingga pemeliharaan kebersihan mulut yang tepat selama perawatan ortodonti dapat dilakukan. Kata Kunci: ortodonti cekat, perawatan gigi, kebersihan mulut, pandemi, droplet, aerosol
Hubungan Tingkat Stres dengan Stomatitis Aftosa Rekuren pada Mahasiswa Universitas Mulawarman: Correlation Between Stress Level and Reccurent Aphtous Stomatitis on Mulawarman University’s Students Hanida Fitri Hasanah; Verry Asfirizal; Silfra Yunus Kende; Masyhudi Masyhudi; Cicih Bhakti Purnamasari
Jurnal Sains dan Kesehatan Vol. 5 No. 2 (2023): J. Sains Kes.
Publisher : Fakultas Farmasi, Universitas Mulawarman, Samarinda, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25026/jsk.v5i2.1726

Abstract

Recurrent aphthous stomatitis (SAR) is an oral disease characterized by recurrent ulcers limited to the oral mucosa without other systemic disorders in the patient. SAR can create discomfort and disturbance, especially when performing the functions of mastication, swallowing, and speech. There are predisposing factors that trigger RAS, one of which is stress. This study aimed to determine the relationship between stress levels and the incidence of Recurrent Aphthous Stomatitis (SAR) in undergraduate students at Mulawarman University.  This research is a cross-sectional study with a purposive sampling technique. The sample was 380 Mulawarman university students. The data analyses used were univariate and bivariate analyses with a chi square test.  The results showed that 82% of students (311 students) were in the moderate category, and 61% (231 students) did not experience RAS. The chi-square test showed no significant relationship between mild and moderate stress levels and moderate and severe stress on SAR. There was a significant relationship between mild and severe stress levels on SAR, where students with mild stress levels tended not to experience SAR 1.731 times compared to students with severe stress. There was a relationship between stress levels and recurrent aphthous stomatitis in Mulawarman University students. Keywords: RAS, Stress, Students Abstrak Salah satu penyakit mulut yang sering terjadi ialah stomatitis aftosa rekuren (SAR), dengan karakteristik ulkus berulang terbatas pada mukosa mulut tanpa gangguan sistemik lainnya pada pasien. SAR dapat menyebabkan rasa tidak nyaman dan terganggu, terutama pada saat melakukan fungsi pengunyahan, penelanan, dan berbicara. Sejumlah faktor presdisposisi diduga memicu terjadinya SAR, diantaranya yaitu stres. Tujuan penelitian ini untuk mengetahui hubungan tingkat stres dengan kejadian Stomatitis Aftosa Rekuren (SAR) pada mahasiswa sarjana dan diploma Universitas Mulawarman. Penelitian ini merupakan studi cross-sectional dengan teknik pengambilan sampel purposive sampling dengan sampel sebanyak 380 mahasiswa universitas Mulawarman, analisis data menggunakan analisis univariat dan bivariat dengan uji chi-square. Hasil penelitian menyatakan stres pada mahasiswa sebesar 82% (311 mahasiswa) berada pada kategori sedang dan sebanyak 61% (231 mahasiswa) tidak mengalami SAR. Uji chi-square menunjukkan tidak ada hubungan yang signifikan antara tingkat stres ringan dan sedang maupun stres sedang dan berat terhadap SAR dan terdapat hubungan yang signifikan antara tingkat stres ringan dan berat terhadap SAR, dimana mahasiswa dengan tingkat stres ringan cenderung tidak mengalami SAR 1,731 kali lipat dibandingkan mahasiswa dengan stres berat. Terdapat hubungan antara tingkat stres dengan stomatitis aftosa rekuren pada mahasiswa Universitas Mulawarman. Kata Kunci: SAR, Stres , Mahasiswa