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All Journal Jurnal Sain Veteriner Buletin Peternakan Jurnal Veteriner JOURNAL OF COASTAL DEVELOPMENT Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati IGKOJEI: Jurnal Pengabdian Masyarakat
Hastari Wuryastuty
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Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Education Social Sciences Veterinary Other

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Journal : Jurnal Veteriner

 1 
Avian Influenza Virus H5N1 Remained Exist in Gastrointestinal Tracts of House Flies 24 Hours Post-infection) (VIRUS FLU BURUNG H5N1 TETAP BERADA DALAM SALURAN PENCERNAAN LALAT RUMAH 24 JAM PASCAINFEKSI) Tisna Tyasasmaya; Hastari Wuryastuty; Wasito Wasito; Kai Sievert
Jurnal Veteriner Vol 17 No 2 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

House flies (Musca domestica L.) are one of the major pests found in poultry farms resulting in not onlyannoyance and indirect damage to the poultry production but also transmitting many infectious organismsmechanically and biologically. A highly pathogenic avian influenza virus H5N1 (HPAIV H5N1) collectedfrom field house flies in Java Island, have fully succeeded identified and isolated in 2008. The AIV H5N1isolates were further used in the present study to determine the presence and persistence of the AIV H5N1in the laboratory infected house flies. One hundred house flies from a free AIV poultry farm in Yogyakarta,Indonesia were used in this study. The collected house flies were fasted for 12 hours then divided equallyin control and treated groups. The treated group was allowed to imbibe Dulbecco’s modified eagle medium(DMEM) containing AIV H5N1 while the control group imbibed medium without virus for one hour. Theflies from each group were collected at 12 and 24 hours post-exposure, respectively. All flies were immobilizedat 4°C, immersed in absolute ethanol for a few seconds and dissected under the stereomicroscope to collectthe gastrointestinal (GI) tracts. Based on the RT-PCR results, it is concluded that AIV H5N1 remainedexist in GI tracts of house flies for at least 24 hours post-exposure.
Chitosan Mempertipis Dinding dan Memperbesar Diameter Lumen Arteri Koroner Tikus Putih yang Diberi Pakan Lemak Tinggi (CHITOSAN COULD THINEN WALL AND WIDEN LUMEN DIAMETER CORONARY ARTERY OF SPRAGUE DAWLEY RATS INDUCED BY HIGH FAT RATION) Sri Isdadiyanto; Sukarti Moeljopawiro; Nyoman Puniawati; Hastari Wuryastuty
Jurnal Veteriner Vol 14 No 3 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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The objective of this study was to analyze the influence of chitosan on coronary artery wall thicknessand lumen diameter of Sprague Dawley rats induced by high fat ration. The animals for this study weretwenty adult male rats divided into four groups, i.e. group I as the control was fed with basal rationcontaining normal fat for 3 months, group II was fed ration containing high fat for three months, group IIIwas fed ration containing high fat and given chitosan 180 mg per kg body weight per day orally in 2 mLaquadest for 3 months, group IV was fed ration containing high fat for three months and after one monthgiven chitosan 180 mg per kg body weight per day orally in 2 mL aquadest for two months. Each groupconsisted of five animals. After 90 days, the rats were necropsied and the hearts were collected tohistopathological. The difference between treatments was statistical analysis by Anava, and continued byLowest Significant Difference Test to locate the difference. Normal fat ration did not influence coronaryartery wall thickness and coronary artery lumen diameter in rats. High fat ration increased coronaryartery wall thickness and chitosan could be decreased coronary artery wall thickness in rats. High fatration + chitosan that given simultaneously could be increased coronary artery lumen diameter in rats.Based on the result of this study, it was concluded that high fat ration was a major factor able to causeatherosclerosis and chitosan was able to prevent atheroma plaque formation.
Co-Authors Agnes Murdiati Dhirgo Adji Eni Harmayani Guntari Titik Mulyani Hartiningsih . Hary Purnamaningsih Ida Tjahajati Irkham Widiyono Kai Sievert Laksono Trisnantoro Luh Made Sudimantini Nyoman Puniawati Prabowo Purwono Putro R. Wasito Soedarmanto Indarjulianto Soedarmanto Indarjulianto Sri Isdadiyanto Sri Isdadiyanto Sukarti Moeljopawiro Tisna Tyasasmaya Wasito Wasito Y. Marsono