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All Journal Jurnal Sain Veteriner Jurnal Pengabdian dan Pengembangan Masyarakat
Clara Ajeng Artdita
Program Studi Teknologi Veteriner Departemen Teknologi Hayati Dan Veteriner Sekolah Vokasi Universitas Gadjah Mada
Humanities Education Environmental Science Languange, Linguistic, Communication & Media Library & Information Science Veterinary

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Klebsiella pneumoniae Isolated from Subclinical Mastitis Milk of Etawah Crossbreed Goat Clara Ajeng Artdita; Fajar Budi Lestari; Achmad Fauzi; Erian Pemila Ayu Tanzila
Jurnal Sain Veteriner Vol 36, No 2 (2018): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (9907.598 KB) | DOI: 10.22146/jsv.34319

Abstract

Klebsiella spp. is a common bacteria causing mastitis. Klebsiella pneumoniae and Klebsiella varicola is the most infected Klebsiella spp in the dairy farm. This study used 16 milk samples from 8 lactation goats in Sahabat Ternak farm, Sleman, Yogyakarta. Samples were tested by california mastitis test (CMT) reagent to determine the mastitis status, inoculated on blood agar (BA) then on Mac Conkey Agar (MCA) as a selective media for Gram negative bacteria. The colonies from BA and MCA were stained by Gram staining to determine Gram-positive or Gram-negative bacteria and its cell morphology. The colonies from MCA were identified by biochemical tests such as sugar fermentation tests (glucose, lactose, maltose, and saccharose), arginine, ornithine decarboxylase, indole, citrate, urea hydrolysis (Christensen’s method), lysin and malonate. The result of mastitis test showed 5 goats were positive result and 3 samples were negative. Bacterial staining showed 2 samples were Gram-negative, rods and the others were Gram-positive, coccus. The samples with rods shapes were continued by biochemical tests. The characterization result of biochemical test indicated that the rods shapes bacteria were Klebsiella pneumoniae. These bacteria identification conducted in Laboratorium Preklinis Program Studi Kesehatan Hewan, Sekolah Vokasi Universitas Gadjah Mada and the result confirmation using positive control of  Klebsiella pneumoniae conducted in Balai Laboratorium Kesehatan Yogyakarta. These results showed that milk from Etawah crossbreed goat was infected by Klebsiella pneumoniae.
Bacterial Detection Causing Subclinical Mastitis on Etawah Crossbreed Goat in Kokap, Kulon Progo, Yogyakarta Province Clara Ajeng Artdita; Morsid Andityas; Nur Ika Prihanani; Yohanes Wawan Budiyanto
Jurnal Sain Veteriner Vol 38, No 1 (2020): April
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.39818

Abstract

Resident in Kokap, Kulon Progo, Yogyakarta Province has another income sector, that is dairy goat (Etawah Crossbreed) farming and they have been joining into farm group, which is called Kelompok Ternak. There are many bacteria that causing dairy goat intra mamary infection, thus the milk production will be contaminated. Two hundred and four (204) milk samples from 103 lactation goat in four farm groups in Kokap, Kulon Progo, Yogyakarta is used in this study. Out of these, 21 samples (10,29%) were detected strong positive (++ and +++) for subclinical mastitis by using California Mastitis Test (CMT). Among this positive result, the next step was bacteria detection. This tests were held in Preclinic Laboratory Animal Health Study Program, Vocational School, UGM. The samples were inoculated in blood agar, then colony was identify by Gram staining. The next steph is biochemical tests using McConkey Agar (MCA) and eosin methylene blue (EMB) as a selective media for Gram negative bacteria,  manitol salt agar (MSA) as selective media for Staphylococcus, sugar fermented broth (glucose, lactose, mannitol, maltose, and sacharose), Kigler iron agar (KIA), lysine iron agar (LIA), motility indole ornithin (MIO), and Simmon’s citrate. The result was Staphylococcus aureus (80%), Staphylococcus epidermidis (20%), Escherichia coli (10%), and Klebsiella pneumoniae (10%). 
Identifikasi Molekuler Bakteri Staphylococcus sp. dan Staphylococcus aureus Penyebab Mastitis Subklinis pada Ternak Kambing Perah Clara Ajeng Artdita; Fatkhanuddin Aziz; Nurulia Hidayah; Achmad Fauzi; Triastuti Septi Wulandari; Reza Luthfi Hamid
Jurnal Sain Veteriner Vol 39, No 2 (2021): Agustus
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.60557

Abstract

Mastitis merupakan radang pada glandula mammae (ambing) ternak perah. Mastitis tipe subklinis sering dikaitkan pada kejadian mastitis di peternakan ruminansia kecil seperti kambing perah (kambing Peranakan Etawah, Saanen, dan Sapera). Patogen utama yang berperan dalam kejadian mastitis ini adalah genus Staphylococcus. Tujuan penelitian adalah untuk melakukan identifikasi bakteri Staphylococcus sp. dan Staphylococcus aureus sebagai penyebab mastitis subklinis pada kambing perah dengan menggunakan metode polymerase  chain reaction (PCR). Tahapan metode yang dilakukan adalah ekstraksi DNA dengan teknik spin-collumn system terhadap 26 isolat bakteri yang telah dilakukan uji biokimia sebelumnya dan amplifikasi gen spesifik 23s rRNA Staphylococcus sp. dan Staphylococcus aureus, serta methicillin resistant Staphylococcus aureus (MRSA), dilanjutkan dengan visualisasi menggunakan UV-transluminator. Hasil menunjukkan bahwa sebanyak 12 isolat sampel teridentifikasi Staphylococcus sp. dan 1 diantaranya teridentifikasi Staphylococcus aureus. Isolat yang teridentifikasi Staphylococcus aureus bukan termasuk MRSA. 
Pembentukan dan Pembinaan Kelompok Tani Kelinci dalam Rangka Pengembangan Desa Wisata di Soropati, Hargotirto, Kokap, Kulon Progo Naela Wanda Yusria Dalimunthe; Clara Ajeng Artdita; Fajar Budi Lestari
Jurnal Pengabdian dan Pengembangan Masyarakat Vol 2, No 1 (2019): MAY
Publisher : Pengabdian dan Pengembangan Masyarakat Sekolah Vokasi UGM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jp2m.48334

Abstract

Community Service Activities in 2018 have been implemented in Soropati Village, Hargotirto, Kokap, Kulon Progo. The purpose of this activity was to provide information on the maintenance and formation of rabbit farmer groups by involving the academics of the Diploma in Animal Health Program, Department of Biological and Veterinary Technology, Vocational School, Gadjah Mada University with Animal Health Centre in Kokap so that a better  communication between academics, officers, and breeders community should be conducted well. The activity is carried out by providing information and training transfers regarding the management of rabbit maintenance including rabbit species, housing management, feed, and breeding. The implementation of this program was carried out by giving professional speech and interactive discussions then comparative studies to rabbit farmers who had been successful, counseling and fostering. The result of this activity is that the Mantep Makaryo farmer group has been formed which is engaged in the maintenance and cultivation of rabbits. Development of sustainable breeders needs to be carried out to improve health management and the development of rabbit farms.
Co-Authors Achmad Fauzi Achmad Fauzi Erian Pemila Ayu Tanzila Fajar Budi Lestari Fajar Budi Lestari Fatkhanuddin Aziz, Fatkhanuddin Morsid Andityas Naela Wanda Yusria Dalimunthe Nur Ika Prihanani NURULIA HIDAYAH Reza Luthfi Hamid Triastuti Septi Wulandari Yohanes Wawan Budiyanto