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All Journal Jurnal Sain Veteriner Indonesian Journal of Biotechnology JURNAL ILMIAH PETERNAKAN TERPADU Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Sciences)
Fatkhanuddin Aziz, Fatkhanuddin
Program Studi Teknologi Veteriner Departemen Teknologi Hayati Dan Veteriner Sekolah Vokasi Universitas Gadjah Mada
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Economics, Econometrics & Finance Immunology & microbiology Materials Science & Nanotechnology Veterinary Other

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Genetic Determination and Clonal Relationships of Staphylococcus aureus Isolated from Dairy Cows in Baturraden, Central Java, Indonesia Fatkhanuddin Aziz; Siti Isrina Oktavia Salasia; Mitra Slipranata
Indonesian Journal of Biotechnology Vol 19, No 2 (2014)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (243.496 KB) | DOI: 10.22146/ijbiotech.9302

Abstract

Cases of mastitis in cows at Baturraden are leading to signifi cant and ongoing problems due to reducedproduction and lower milk quality. This study was designed to identify which of selected virulence determinantgenes of S. aureus are involved in the Baturraden infection, and to determine the clonal relationship amongthese isolates. Seventeen isolates were identifi ed as S. aureus based on their biochemical properties and speciesspecifi city for 23S rRNA and nuc genes. S. aureus isolates were genotypically characterized for the selectedvirulence determinants: coa, clfA, fnbA, fnbB, cap5, spa IgG and spa X- region genes. Clonal relationship analysisamong isolates was carried out using AFLP and results compared with previously confi rmed relationshipsbetween selected S. aureus isolated from other regions. The results show that eight isolates contain all thegenes, but six isolates lack fnbB and two isolates lack cap5 genes. AFLP analysis showed that all isolates of S.aureus originating from cows in Baturraden belong to one cluster. This study provides additional knowledgeabout S. aureus infection in Baturraden cows, including the number of virulence determinant genes that mayplay a role in pathogenicity.
Deteksi Gen Penyandi Sifat Resistensi Metisilin, Penisilin dan Tetrasiklin pada Isolat Staphylococcus aureus Asal Susu Mastitis Subklinis Sapi Perah Fatkhanuddin Aziz; Fajar Budi Lestari; Sarah Nuraidah; Endah Purwati; Siti Isrina Oktavia Salasia
Jurnal Sain Veteriner Vol 34, No 1 (2016): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (620.058 KB) | DOI: 10.22146/jsv.22816

Abstract

Detection of gene encoding resistance of bacteria could be used as an accurate method to determine resistance of Staphylococcus aureus which is causing mastitis in dairy cows to the several antibiotics. This research aimed to detect the gene encoding resistance of methicillin, penicillin and tetracycline from identified S. aureus. Sixty milk samples were collected from subclinical mastitis of cows from various dairy farming in Yogyakarta. Isolation and identification of S. aureus based on the culture, Gram staining and biochemical test. Phenotypes of S. aureus resistances against antibiotics were carried out by disc diffusion method, meanwhilespecies specific gene of S. aureus and the gene encoding methicillin, penicillin and tetracycline were confirmed by PCR method. The results showed 11 isolates representing of Methicillin Susceptible Staphylococcus aureus (MSSA) could be identified, wherein 5 isolates were harboring both of penicillin and tetracycline resistant genes respectively.
Deteksi Staphylococcus aureus dan Staphylococcus sp. Secara Langsung Dari Susu Segar Kambing Peranakan Etawa dengan Teknik PCR Fatkhanuddin Aziz; Fajar Budi Lestari; Sarah Nuraida S; Endah Purwati; Siti Isrina Oktavia Salasia
Jurnal Sain Veteriner Vol 38, No 2 (2020): Agustus
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.53802

Abstract

Genus Staphylococcus merupakan salah satu patogen bakteri penyebab mastitis yang menyebabkan kerugian ekonomi pada kambing Peranakan Etawa. Diantara Staphylococcus sp. yang dapat tumbuh dengan baik dalam susu segar, diketahui Staphylococcus aureus dapat membahayakan kesehatan manusia yang mengkonsumsi (food borne disease) karena kemampuannya dalam memproduksi enterotoksin yang tahan terhadap enzim pencernaan maupun pemanasan. Tujuan penelitian ini adalah mendeteksi Staphylococcus sp. dan S. aureus secara langsung dari susu kambing peranakan etawa dengan teknik PCR.Metode yang dilakukan adalah mengekstraksi DNA dari 60 sample susu segar dengan prinsip spin column-based nucleic acid purification dan kemudian dilakukan amplifikasi gen spesifik 23S rRNA Staphylococcus sp. dan S. aureus. Hasil PCR diketahui 37 (61%) sampel susu positif mengandung Staphylococcus sp. dan hanya 1 (1,6%) sampel mengandung S. aureus. Metode deteksi dengan PCR dapat digunakan untuk mendeteksi kontaminan Staphylococcus sp. dan S. aureus dengan waktu yang singkat
Identifikasi Molekuler Bakteri Staphylococcus sp. dan Staphylococcus aureus Penyebab Mastitis Subklinis pada Ternak Kambing Perah Clara Ajeng Artdita; Fatkhanuddin Aziz; Nurulia Hidayah; Achmad Fauzi; Triastuti Septi Wulandari; Reza Luthfi Hamid
Jurnal Sain Veteriner Vol 39, No 2 (2021): Agustus
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.60557

Abstract

Mastitis merupakan radang pada glandula mammae (ambing) ternak perah. Mastitis tipe subklinis sering dikaitkan pada kejadian mastitis di peternakan ruminansia kecil seperti kambing perah (kambing Peranakan Etawah, Saanen, dan Sapera). Patogen utama yang berperan dalam kejadian mastitis ini adalah genus Staphylococcus. Tujuan penelitian adalah untuk melakukan identifikasi bakteri Staphylococcus sp. dan Staphylococcus aureus sebagai penyebab mastitis subklinis pada kambing perah dengan menggunakan metode polymerase  chain reaction (PCR). Tahapan metode yang dilakukan adalah ekstraksi DNA dengan teknik spin-collumn system terhadap 26 isolat bakteri yang telah dilakukan uji biokimia sebelumnya dan amplifikasi gen spesifik 23s rRNA Staphylococcus sp. dan Staphylococcus aureus, serta methicillin resistant Staphylococcus aureus (MRSA), dilanjutkan dengan visualisasi menggunakan UV-transluminator. Hasil menunjukkan bahwa sebanyak 12 isolat sampel teridentifikasi Staphylococcus sp. dan 1 diantaranya teridentifikasi Staphylococcus aureus. Isolat yang teridentifikasi Staphylococcus aureus bukan termasuk MRSA. 
Antibiotic Resistance Detection of Staphylococcus aureus Isolated from Dairy Cattle and Pet Animals Yosyana, Alyaa Rifqoh Putri; Salasia, Siti Isrina Oktavia; Wasissa, Madarina; Alhadz, Ghias Ghifari; Aziz, Fatkhanuddin
Jurnal Sain Veteriner Vol 43, No 1 (2025): April
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.100211

Abstract

Antibiotics play a significant role in controlling bacterial infection, however, will no longer be effective because of antimicrobial resistance (AMR). Staphylococcus aureus has become resistant to various antibiotics, so detecting and analyzing genes encoding antibiotic resistance traits is important. This research aims to identify phenotypically, antibiotic sensitivity, and detect antibiotic-resistant genes in S. aureus isolated from dairy cattle and pet animals. Samples from dairy farms in Boyolali total of 30 samples and 62 samples of pet animals in Yogyakarta and Semarang were used. Phenotypic and genotypic identification results based on 23S rRNA and nuc genes showed 80% (24/30) dairy milk samples and 19,35% (12/62) pet animal samples were S. aureus positive. Based on antibiotic susceptibility test, dairy milk S. aureus isolates are resistant to penicillin G (50%), oxacillin (25%), tetracycline (21%), ampicillin (17%), gentamicin, cefoxitin, and amoxicillin (13%), clindamycin (4%), and still sensitive to erythromycin (100%). Pet animal S. aureus isolates showed resistance to oxacillin and erythromycin (13,3%), tetracycline, penicillin G, and clindamycin (6,67%), but still sensitive to gentamicin, ampicillin, cefoxitin, and ciprofloxacin and amoxicillin (100%). These results showed S. aureus dairy milk and pet animal isolates phenotypically have resistance almost 50% to various antibiotics but are still sensitive to erythromycin. The result of this research indicated there are majority of multidrug-resistant S. aureus strains in dairy milk and pet animals threaten public health. These results can be used as a basic strategy for controlling and preventing multidrug resistance in S. aureus.
Evaluation of molecular primers for sexing the magpie robin and green cucak via CHD1 gene amplification Fuadah, Khilmi; Rahmawati, Anisa; Fitriana, Fauziah; Aziz, Fatkhanuddin
Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Science) Vol. 15 No. 2 (2025): Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Ve
Publisher : Fakultas Peternakan Universitas Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46549/jipvet.v15i2.557

Abstract

Researchers and breeders are increasingly using the Polymerase Chain Reaction (PCR) method for bird sexing. However, the compatibility of the DNA template and the oligo primer used is the primary key to the success of PCR amplification. In the present study, we evaluated five types of popular PCR primers sexing to determine the sex of a pair of each the magpie robin (Copsychus malabaricus) and green cucak (Chloropsis sonnerati). We used DNA samples from each pair of males and females from the two species above, respectively, to test the five primers listed to amplify the target gene chromodomain helicase DNA binding 1 (CHD1). The results demonstrated that the primer sets of CHD1LF/CHD1LR, CHD1F/CHD1R, and P2/P8 could be used for Copsychus malabaricus, while only CHD1LF/CHD1LR was suitable for Chloropsis sonnerati. It is crucial to select the correct primer to improve bird sexing accuracy because several of the other primers examined in this study were unable to amplify the targeted CHD1 gene correctly.
Biofilm Properties and Their Association to Antibiotic Resistance of Staphylococcus aureus from Animal Isolates Aziz, Fatkhanuddin; Fitriana, Fauziah; Setyorini, Dian Ritma; Prihanani, Nur Ika; Andityas, Morsid
JURNAL ILMIAH PETERNAKAN TERPADU Vol. 13 No. 2 (2025)
Publisher : DEPARTMENT OF ANIMAL HUSBANDRY, FACULTY OF AGRICULTURE, UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jipt.v13i2.p558-571

Abstract

Staphylococcus aureus (S. aureus) is a Gram positive bacterium that has many virulence factor including the ability to produce biofilm. Biofilm formation is one of the important ability in the persistance and resistant to antibiotic treatment. This study aimed to determine the phenotypic and genotypic characteristics of S. aureus isolates from animal origin in their capacity to produce biofilm in vitro. Eight S. aureus isolates collection from goat mastitis and raw chicken meat origin were phenotypically evaluated the ability to produce biofilm in 96 well culture plate, while genotypic were determinated by detecting the icaA, icaC and icaD genes. We employed  minimum of inhibitory concentration (MIC) data from previous research to clarify their correlation to biofilm production in respected isolates. The results showed that 75% (6/8) of S. aureus isolates had ability to produced biofilm, whereas 50% (4/8) showed the elevation of biofilm production after glucose was added. PCR determination showed that majority isolates were positive for icaA, icaC and icaD genes, while one of the isolates was negative for the icaA. The statistical analysis tests indicated no correlation between the optical density of biofilm production and MIC of antibiotics. Further research is needed to clarify the association of biofilm and antibiotic resistance.
Komparasi Metode coa Polymorphism dan coa Typing pada Bakteri Staphylococcus aureus yang Diisolasi dari Sumber Berbeda Aziz, Fatkhanuddin; Fitriana, Fauziah; Setyorini, Dian Ritma; Prihanani, Nur Ika; Putri, Shafira Amalia; Maulina, Tifa Restyka; Dewi, Vira Kartika; Andityas, Morsid; Lestari, Fajar Budi; Hidayah, Nurulia; Ummami, Risa; Fauzi, Achmad
Jurnal Sain Veteriner Vol 42, No 1 (2024): April
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.89815

Abstract

Staphylococcus aureus (S. aureus) merupakan golongan bakteri koagulase positif yang menyebabkan berbagai macam penyakit klinis pada manusia dan hewan. Determinasi pola gen coa merupakan salah satu metode yang sering digunakan dalam lingkup studi epidemiologi S. aureus. Penelitian ini dilakukan untuk membandingkan metode coa polymorphism dan coa typing pada isolat S. aureus yang diisolasi dari sumber berbeda. Tujuh belas koleksi isolat S. aureus asal susu pasteurisasi, susu mastitis kambing peranakan etawa, dan daging ayam segar ditumbuhkan dari stok gliserol -80oC, kemudian dilakukan ekstraksi DNA dan amplifikasi gen penyandi koagulase (coa) dengan teknik PCR menggunakan primer untuk coa polymorphism dan coa typing. Diketahui, level diskriminasi coa typing lebih variatif dibandingkan dengan coa polymorphism pada 17 isolat yang diuji. Metode coa typing menunjukkan 7 tipe berbeda, sedangkan coa polymorphism hanya 3. Empat dari 17 isolat tidak dapat ditentukan  coa typing, menunjukkan potensi pengembangan tipe baru coa typing untuk isolat-isolat asal Indonesia, untuk kepentingan studi epidemiologi.
Co-Authors Achmad Fauzi Achmad Fauzi Alhadz, Ghias Ghifari Anisa Rahmawati Clara Ajeng Artdita Dewi, Vira Kartika Endah Purwati Endah Purwati Fajar Budi Lestari Fitriana, Fauziah Fuadah, Khilmi Hidayah, Nurulia Maulina, Tifa Restyka Mitra Slipranata Morsid Andityas NURULIA HIDAYAH Prihanani, Nur Ika Putri, Shafira Amalia Reza Luthfi Hamid Risa Ummami, Risa Sarah Nuraida S Sarah Nuraidah Setyorini, Dian Ritma Siti Isrina Oktavia Salasia Siti Isrina Oktavia Salasia Siti Isrina Oktavia Salasia Triastuti Septi Wulandari Wasissa, Madarina Yosyana, Alyaa Rifqoh Putri