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Deteksi Bakteri Penyebab Mastitis Subklinis pada Kambing Peranakan Etawah di Kokap, Kulon Progo, Daerah Istimewa Yogyakarta Artdita, Clara Ajeng; Andityas, Morsid; Prihanani, Nur Ika; Budiyanto, Yohanes Wawan
Jurnal Sain Veteriner Vol 38, No 1 (2020): April
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Resident in Kokap, Kulon Progo, Yogyakarta Province has another income sector, that is dairy goat (Etawah Crossbreed) farming and they have been joining into farm group, which is called Kelompok Ternak. There are many bacteria that causing dairy goat intra mamary infection, thus the milk production will be contaminated. Two hundred and four (204) milk samples from 103 lactation goat in four farm groups in Kokap, Kulon Progo, Yogyakarta is used in this study. Out of these, 21 samples (10,29%) were detected strong positive (++ and +++) for subclinical mastitis by using California Mastitis Test (CMT). Among this positive result, the next step was bacteria detection. This tests were held in Preclinic Laboratory Animal Health Study Program, Vocational School, UGM. The samples were inoculated in blood agar, then colony was identify by Gram staining. The next steph is biochemical tests using McConkey Agar (MCA) and eosin methylene blue (EMB) as a selective media for Gram negative bacteria, manitol salt agar (MSA) as selective media for Staphylococcus, sugar fermented broth (glucose, lactose, mannitol, maltose, and sacharose), Kigler iron agar (KIA), lysine iron agar (LIA), motility indole ornithin (MIO), and Simmonâ€™s citrate. The result was Staphylococcus aureus (80%), Staphylococcus epidermidis (20%), Escherichia coli (10%), and Klebsiella pneumoniae (10%).Â

Isolasi dan identifikasi Escherichia coli dari Sumber Air Minum Kandang Broiler serta Uji Aktivitas Antibakteri Lidah Buaya Nuraini, Dian Meididewi; Andityas, Morsid; Paramarta, Adi; Najib, Nur Rohman; Wijayanti, Agustina Dwi
Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Science) Vol 10 No 2 (2020): Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Vete
Publisher : Fakultas Peternakan Universitas Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46549/jipvet.v10i2.116

Abstract Colibacillosis is one of the most problematic issues in the boiler industry. However, the antibiotic overuse has induced Escherichia coli resistance so that other alternative to reduce colibacillosis is needed. One of the alternatives is using aloe vera (Aloe barbadensis Miller), which has been widely used as an antibacterial agent. This study aims to isolate and identify E. coli from the broiler drinking water source and test the aloe vera antibacterial activity against it. Escherichia coli were isolated from well in three broiler farms in Moyudan District, Sleman, Yogyakarta that previously had colibacillosis. Escherichia coli were isolated using eosin methylene blue (EMB) agar and the metallic sheen colony was tested to confirm the biochemist reaction. The pure isolate of E. coli was used in the aloe vera inhibition test using Muller Hinton agar (MHA) by a Well Diffusion method. Aloe vera was processed using aquades and ethanol 70%. The aquades infusion was diluted into 12.5%, 25%, 50%, 75%, and 100% and the extract ethanol 70% was diluted into 10%, 12.5, %, 25%, 40%, and 50%. The bacterial identification showed that one of three samples contained E. coli which was then used for inhibition test. The result showed no inhibition zone in the aquades infusion while ethanol extract showed an inhibition zone in concentration 25%, 40%, and 50% of aloe vera extract with a diameter 19.5 mm, 24 mm, and 25 mm. It can be concluded that aloe vera ethanol extract has inhibitory activity against E. coli in poultry drinking water with a minimum concentration of 25%. Keywords: Aloe vera; Broiler drinking water; Escherichia coli; Inhibitory activity Abstrak Colibacilosis masih menjadi permasalahan dalam industri broiler. Penggunaan antibiotik berlebihan telah menyebabkan resistensi sehingga perlu alternatif lain. Salah satu alternatif adalah menggunakan bahan alami seperti adalah lidah buaya (Aloe barbadensis Miller) yang memilliki aktivitas antibakteri. Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi E. coli dari sumber air minum di kandang broiler serta menguji aktivitas inhibisi lidah buaya terhadap bakteri tersebut. Air yang digunakan sebagai sumber E. coli berasal dari sumur di tiga peternakan broiler di Kecamatan Moyudan, Sleman, Yogyakarta yang memiliki riwayat infeksi colibacilosis. Bakteri E. coli diisolasi menggunakan media eosin methylene blue (EMB) dan diuji sifat biokimia untuk mengkonfirmasi sifat bakteri E. coli. Isolat murni E. coli digunakan pada uji daya hambat bakteri dengan metode difusi sumuran menggunakan media Muller Hinton Agar (MHA). Lidah buaya diproses menggunakan aquades dan ethanol 70%. Infusa aquades diencerkan menjadi konsentrasi 12,5%, 25%, 50%, 75%, dan 100% dan ekstrak ethanol 70% diencerkan menjadi 10%, 12., %, 25%, 40%, dan 50%. Hasil isolasi menunjukan bahwa satu sumber air dari sumur di Desa Kolowenang mengandung E. coli yang kemudian digunakan pada pengujian daya hambat. Hasil pengujian menunjukan tidak ada daya hambat yang terbentuk pada infusa aquades sedangkan ekstrak etanol lidah buaya 25%, 40%, dan 50% menunjukan adanya zona hambat sebesar 19,5 mm, 24 mm, dan 25 mm berturut-turut. Ekstrak etanol lidah buaya pada penelitian ini memiliki kemampuan menghambat pertumbuhan E. coli yang bersumber dari air minum broiler dengan konsentrasi terendah 25%. Kata kunci: Air minum broiler; Escherichia coli; Lidah buaya; Daya hambat bakteri

Bacterial Detection Causing Subclinical Mastitis on Etawah Crossbreed Goat in Kokap, Kulon Progo, Yogyakarta Province Clara Ajeng Artdita; Morsid Andityas; Nur Ika Prihanani; Yohanes Wawan Budiyanto
Jurnal Sain Veteriner Vol 38, No 1 (2020): April
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.39818

Resident in Kokap, Kulon Progo, Yogyakarta Province has another income sector, that is dairy goat (Etawah Crossbreed) farming and they have been joining into farm group, which is called Kelompok Ternak. There are many bacteria that causing dairy goat intra mamary infection, thus the milk production will be contaminated. Two hundred and four (204) milk samples from 103 lactation goat in four farm groups in Kokap, Kulon Progo, Yogyakarta is used in this study. Out of these, 21 samples (10,29%) were detected strong positive (++ and +++) for subclinical mastitis by using California Mastitis Test (CMT). Among this positive result, the next step was bacteria detection. This tests were held in Preclinic Laboratory Animal Health Study Program, Vocational School, UGM. The samples were inoculated in blood agar, then colony was identify by Gram staining. The next steph is biochemical tests using McConkey Agar (MCA) and eosin methylene blue (EMB) as a selective media for Gram negative bacteria, manitol salt agar (MSA) as selective media for Staphylococcus, sugar fermented broth (glucose, lactose, mannitol, maltose, and sacharose), Kigler iron agar (KIA), lysine iron agar (LIA), motility indole ornithin (MIO), and Simmon’s citrate. The result was Staphylococcus aureus (80%), Staphylococcus epidermidis (20%), Escherichia coli (10%), and Klebsiella pneumoniae (10%).

Profil Vulva dan Suhu Tubuh Kambing Peranakan Etawa pada Sinkronisasi Estrus Menggunakan Medroxy Progesterone Acetate dan Suplementasi Zinc (Zn) Muhammad Rosyid Ridlo; Risa Ummami; Naela Wanda Yusria Dalimunthe; Dhasia Ramandani; Nur Ika Prihanani; Morsid Andityas; Tri Satya Mastuti Widi
Jurnal Nasional Teknologi Terapan (JNTT) Vol 2, No 2 (2018): JULI
Publisher : Penelitian dan Pengabdian Kepada Masyarakat Sekolah Vokasi Universitas Gadjah Mada

Peranakan Etawa (PE) goat farming is one of the growing livestock sectors in Indonesia. Indonesia needs a sustainable breeding system that can guarantee the availability of goats. Estrus synchronization is a necessity that can help uniform the breeding time and manage the time of pregnancy and birth. Artificial estrus synchronization techniques that use a combination of Prosterone and Zinc minerals give good results of estrus synchronization in goats. The Aim of this research is to understand the combination efectiveness of estrus synchronization technique with progesterone acetate and Zinc supplementation. The design of this study used two treatment groups, each group consisting of three does PE goats. Estrus synchronization of experimental animals in treatment 1 and 2 was carried out using polyurethane sponge implant method which contained medroxi progesterone acetate 60 mg. Group 1 was given Sponge Progesterone for nine 9 days while giving Zinc (Zn) mineral supplement (Elemental Zn 20 mg, Kimia Farma) orally given 7 times. The frequency of Zinc administration every day begins during intra vaginal sponge insertion. Group 2 was treated same as group 1 with 14 days of implant time and 7 times of Zinc supplementation.The results showed that both treatments occurred estrus simultaneously with good estrus quality. Both treatments show the results of the estrus 8 score assessment with the estrus 3-9 range. The maximum estrus quality’s value of group 2 was higher (8.33 ± 0.57) compared to group 1 (8.0 ± 1.0). The estrus duration of group 2 occurs for 24 hours and group 1 for 7 hours. Estrus onset of Group 2 is earlier (58 hours) than group 1 (75 hours). Both treatment groups showed an increase in temperature at period of the occurrence of estrus. Body temperature during estrus time was 38.7 ± 0.20 0C to 39.60 ± 0.26 0C. The conclusion of this study is that in both treatments of estrus synchronization using sponge progesterone and zinc supplementation can cause estrus synchronization effectively . The duration of estrus in group 1 (9 days of implant) was Shorter than group 2 (14 days of implant). The onset of estrus time of group 1 (9 days of implant) was longer than Group 2 (14 days of implant). The body temperature of the experimental animals increases with the occurrence of estrus.

PENDAMPINGAN KEGIATAN DIPPING PUTING PADA TERNAK KAMBING PERAH DI DESA TURGO, TURI, SLEMAN, YOGYAKARTA Clara Ajeng Artdita; Morsid Andityas; Ronny Martien; Maria Advensia Eka Setyaningrum; Achmad Fauzi
Bahasa Indonesia Vol 17 No 01 (2020): Sarwahita : Jurnal Pengabdian Kepada Masyarakat
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21009/sarwahita.171.01

Abstract Dipping is one of the main process for the stage of milking process and it serves to prevent the the bacteria infected the udder on post-milking process. Mastitis can occur because of this stage not being carried out. The program partner is in the Turgo village, Turi, Sleman, Yogyakarta. This partner has obstacles in the magement of Ettawa crossbreed (PE) goat productivity including milking goat Standard Operating Management (SOP). The purpose of t his community service is to make farmer feel easier to do the dipping method by using herbal-based products sprayer to be applied to PE goats after milking to prevent occurence of mastitis. The method of this program is introducing PE goat cultivation by applying the concept of good farming practices and the importance of preventing mastitis, utilizing home yard to plant Piper betle linn. As a basis for making teat dip sprayer, and improving the productivity of PDE goat as dairy goat with herbal teat dip sprayer using ionic gelation method. Teat dip sprayer technology on dairy goats is expected to increase the productivity of PE goat milk in this partner “Kelompok Ternak”. The result is an increasing of farmer’s understanding and the sustainability of milking SOP of dipping with herbal products which easy to apply (sprayer); So that the livestock health and productivity are increases, then it will impact in economic of this farmer community.Abstrak Dipping merupakan proses pencelupan puting ambing ternak perah yang termasuk ke dalam tahapan proses pemerahan susu dan berfungsi untuk mencegah berkembangnya bakteri paska pemerahan. Kasus mastitis dapat terjadi akibat tahapan ini tidak dilakukan usai pemerahan. Mitra program ini adalah kelompok ternak di desa Turgo, Turi, Sleman, Yogyakarta. Mitra ini memiliki kendala dalam manajemen pengelolaan kambing PE produktif termasuk Standar Operasional Prosedur (SOP) dalam pemerahan susu kambing. Tujuan dari pengabdian ini adalah untuk memudahkan petani peternak dalam melakukan metode dipping dengan produk berbahan herbal untuk diaplikasikan pada ternak perahnya usai dilakukan pemerahan sebagai agen antibakterial untuk mencegah kejadian mastitis. Metode yang akan dilakukan melalui program ini yaitu memperkenalkan budidaya kambing PE dengan menerapkan konsep good farming practices dan pentingnya pencegahan mastitis, memanfaatkan pekarangan rumah untuk ditanami daun sirih hijau sebagai dasar pembuatan dipping sprayer, dan memperbaiki produktivitas ternak perah dengan herbal dipping sprayer menggunakan metode ionic gelation. Pemanfaatan teknologi dipping sprayer pada kambing perah diharapkan dapat meningkatkan produktivitas susu kambing PE di Kelompok Ternak mitra ini. Hasil akhir berupa terbentuknya pemahaman peternak serta keberlanjutan SOP pemerahan berupa dipping dengan produk yang mudah pengaplikasiannya (spray dipping) sehingga ternak sehat, produktivitas meningkat, dan ekonomi masyarakat pedesaan mampu terangkat.

Isolasi dan identifikasi Escherichia coli dari Sumber Air Minum Kandang Broiler serta Uji Aktivitas Antibakteri Lidah Buaya: Isolation and Identification Escherichia coli form Broiler Farm Water Source and Aloe Vera Antibacterial Activity Test Dian Meididewi Nuraini; Morsid Andityas; Adi Paramarta; Nur Rohman Najib; Agustina Dwi Wijayanti
Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Science) Vol. 10 No. 2 (2020): Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Ve
Publisher : Fakultas Peternakan Universitas Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46549/jipvet.v10i2.116

Abstract Colibacillosis is one of the most problematic issues in the boiler industry. However, the antibiotic overuse has induced Escherichia coli resistance so that other alternative to reduce colibacillosis is needed. One of the alternatives is using aloe vera (Aloe barbadensis Miller), which has been widely used as an antibacterial agent. This study aims to isolate and identify E. coli from the broiler drinking water source and test the aloe vera antibacterial activity against it. Escherichia coli were isolated from well in three broiler farms in Moyudan District, Sleman, Yogyakarta that previously had colibacillosis. Escherichia coli were isolated using eosin methylene blue (EMB) agar and the metallic sheen colony was tested to confirm the biochemist reaction. The pure isolate of E. coli was used in the aloe vera inhibition test using Muller Hinton agar (MHA) by a Well Diffusion method. Aloe vera was processed using aquades and ethanol 70%. The aquades infusion was diluted into 12.5%, 25%, 50%, 75%, and 100% and the extract ethanol 70% was diluted into 10%, 12.5, %, 25%, 40%, and 50%. The bacterial identification showed that one of three samples contained E. coli which was then used for inhibition test. The result showed no inhibition zone in the aquades infusion while ethanol extract showed an inhibition zone in concentration 25%, 40%, and 50% of aloe vera extract with a diameter 19.5 mm, 24 mm, and 25 mm. It can be concluded that aloe vera ethanol extract has inhibitory activity against E. coli in poultry drinking water with a minimum concentration of 25%. Keywords: Aloe vera; Broiler drinking water; Escherichia coli; Inhibitory activity Abstrak Colibacilosis masih menjadi permasalahan dalam industri broiler. Penggunaan antibiotik berlebihan telah menyebabkan resistensi sehingga perlu alternatif lain. Salah satu alternatif adalah menggunakan bahan alami seperti adalah lidah buaya (Aloe barbadensis Miller) yang memilliki aktivitas antibakteri. Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi E. coli dari sumber air minum di kandang broiler serta menguji aktivitas inhibisi lidah buaya terhadap bakteri tersebut. Air yang digunakan sebagai sumber E. coli berasal dari sumur di tiga peternakan broiler di Kecamatan Moyudan, Sleman, Yogyakarta yang memiliki riwayat infeksi colibacilosis. Bakteri E. coli diisolasi menggunakan media eosin methylene blue (EMB) dan diuji sifat biokimia untuk mengkonfirmasi sifat bakteri E. coli. Isolat murni E. coli digunakan pada uji daya hambat bakteri dengan metode difusi sumuran menggunakan media Muller Hinton Agar (MHA). Lidah buaya diproses menggunakan aquades dan ethanol 70%. Infusa aquades diencerkan menjadi konsentrasi 12,5%, 25%, 50%, 75%, dan 100% dan ekstrak ethanol 70% diencerkan menjadi 10%, 12., %, 25%, 40%, dan 50%. Hasil isolasi menunjukan bahwa satu sumber air dari sumur di Desa Kolowenang mengandung E. coli yang kemudian digunakan pada pengujian daya hambat. Hasil pengujian menunjukan tidak ada daya hambat yang terbentuk pada infusa aquades sedangkan ekstrak etanol lidah buaya 25%, 40%, dan 50% menunjukan adanya zona hambat sebesar 19,5 mm, 24 mm, dan 25 mm berturut-turut. Ekstrak etanol lidah buaya pada penelitian ini memiliki kemampuan menghambat pertumbuhan E. coli yang bersumber dari air minum broiler dengan konsentrasi terendah 25%. Kata kunci: Air minum broiler; Escherichia coli; Lidah buaya; Daya hambat bakteri

Biofilm Properties and Their Association to Antibiotic Resistance of Staphylococcus aureus from Animal Isolates Aziz, Fatkhanuddin; Fitriana, Fauziah; Setyorini, Dian Ritma; Prihanani, Nur Ika; Andityas, Morsid
JURNAL ILMIAH PETERNAKAN TERPADU Vol. 13 No. 2 (2025)
Publisher : DEPARTMENT OF ANIMAL HUSBANDRY, FACULTY OF AGRICULTURE, UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jipt.v13i2.p558-571

Staphylococcus aureus (S. aureus) is a Gram positive bacterium that has many virulence factor including the ability to produce biofilm. Biofilm formation is one of the important ability in the persistance and resistant to antibiotic treatment. This study aimed to determine the phenotypic and genotypic characteristics of S. aureus isolates from animal origin in their capacity to produce biofilm in vitro. Eight S. aureus isolates collection from goat mastitis and raw chicken meat origin were phenotypically evaluated the ability to produce biofilm in 96 well culture plate, while genotypic were determinated by detecting the icaA, icaC and icaD genes. We employed minimum of inhibitory concentration (MIC) data from previous research to clarify their correlation to biofilm production in respected isolates. The results showed that 75% (6/8) of S. aureus isolates had ability to produced biofilm, whereas 50% (4/8) showed the elevation of biofilm production after glucose was added. PCR determination showed that majority isolates were positive for icaA, icaC and icaD genes, while one of the isolates was negative for the icaA. The statistical analysis tests indicated no correlation between the optical density of biofilm production and MIC of antibiotics. Further research is needed to clarify the association of biofilm and antibiotic resistance.

Komparasi Metode coa Polymorphism dan coa Typing pada Bakteri Staphylococcus aureus yang Diisolasi dari Sumber Berbeda Aziz, Fatkhanuddin; Fitriana, Fauziah; Setyorini, Dian Ritma; Prihanani, Nur Ika; Putri, Shafira Amalia; Maulina, Tifa Restyka; Dewi, Vira Kartika; Andityas, Morsid; Lestari, Fajar Budi; Hidayah, Nurulia; Ummami, Risa; Fauzi, Achmad
Jurnal Sain Veteriner Vol 42, No 1 (2024): April
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.89815

Staphylococcus aureus (S. aureus) merupakan golongan bakteri koagulase positif yang menyebabkan berbagai macam penyakit klinis pada manusia dan hewan. Determinasi pola gen coa merupakan salah satu metode yang sering digunakan dalam lingkup studi epidemiologi S. aureus. Penelitian ini dilakukan untuk membandingkan metode coa polymorphism dan coa typing pada isolat S. aureus yang diisolasi dari sumber berbeda. Tujuh belas koleksi isolat S. aureus asal susu pasteurisasi, susu mastitis kambing peranakan etawa, dan daging ayam segar ditumbuhkan dari stok gliserol -80oC, kemudian dilakukan ekstraksi DNA dan amplifikasi gen penyandi koagulase (coa) dengan teknik PCR menggunakan primer untuk coa polymorphism dan coa typing. Diketahui, level diskriminasi coa typing lebih variatif dibandingkan dengan coa polymorphism pada 17 isolat yang diuji. Metode coa typing menunjukkan 7 tipe berbeda, sedangkan coa polymorphism hanya 3. Empat dari 17 isolat tidak dapat ditentukan coa typing, menunjukkan potensi pengembangan tipe baru coa typing untuk isolat-isolat asal Indonesia, untuk kepentingan studi epidemiologi.

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