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All Journal METAMORFOSA Journal of Biological Sciences Jurnal Pertanian Agros
Bernadina Metboki
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology

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Journal : METAMORFOSA Journal of Biological Sciences

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Kemampuan Antagonis Cendawan Endofit terhadap Rhizoctonia sp. Penyebab Penyakit Busuk Akar Tanaman Sorgum (Sorghum bicolor L.) secara In Vitro Aloysius Rusae; Bernadina Metboki; Blasius Atini
Metamorfosa: Journal of Biological Sciences Vol 5 No 2 (2018)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2018.v05.i02.p09

Abstract

Sorgum (Sorgum bicolor L.) is a serelia plant which is potential to becultivated and grown in marginal areas. The advantages of sorghum lie in its extensive agroecological adaptability and resistance to drought. This commodity has a high nutrient content, so it is very good to be used as a source of food and alternative animal feed. Rhizoctonia sp. The cause of root rot is one of the important pathogens in sorghum plants. These pathogens can survive in the soil and can also survive in seeds. Microorganism that developed symbiosis with Zorgum is useful for growth stimulation and biocontrol. The objectives of the researcha re toidentify endofit mushroom that developed symbiosis on some varieties of zorgum and to make an antagonis test on Rhizoctonia sp, the cause of spoiled root disease. This research is conducted in several streas: firstly, take the sample of same varieties of zorgum lokal merah,lokal putih,and numbu in Naiola in Kecamatan Bikomi Selatan while the isolation and identification are held in Faculty of Agrociture of University of Timor and secendly, do antagonist test on Rhizoctonia sp. The study on lokal merah variety has found 7 izolats: Aspergilus sp 1, Aspergilus sp 2, Aspergilus sp 3, Fusarium sp 1, Fusarium sp 2, Trichoderma sp 2. Mushroom endofits found in lokal putih variety are Aspergilus sp.2, Aspergilus sp.3, Fusarium sp.1, dan Trichoderma sp.2. Mushrooms on numbu variety are Fusarium sp. 2, Aspergilus sp.2 dan Trichoderma sp.1. Endofit Mushroom from theroot ofzorgum has 3 genus Aspergilus sp, Fusarium sp dan Trichoderma sp. Trichoderma sp 1 has 20,1 % antagonist character.
EFEKTIVITAS EKSTRAK KULIT BATANG AMPUPU (Eucalyptus alba Reinw. Ex. Blume) DALAM MENGHAMBAT PERTUMBUHAN JAMUR Fusarium sp. PENYEBAB BUSUK TONGKOL JAGUNG (Zea mays L.) Bernadina Metboki; Ni Putu Adriani Astiti; Meitini Wahyuni Proborini
Metamorfosa: Journal of Biological Sciences Vol 3 No 2 (2016)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2016.v03.i02.p01

Abstract

The fungal-related diseases were responsible as one of many reasons for low production of maize in Indonesia. Controlling fungal contaminants using chemical is hazardous for human nowadays. Thus the need of environmental friendly vegetative-based fungicide such as from the extract of Ampupu tree bark is important. The objective of this study was to investigate the antifungal effect from Ampupu tree bark crude extract in response to growth of Fusarium sp, which responsible as the causal agent of corncob rot. The inhibition test on growth of Fusarium sp. by Ampupu tree bark crude extract was performed with diffusion well method on PDA as media. The process involved the infusion of crude extracts at 1.5%, 3.0% and 4.5% concentration, respectively, into diffusion well on each Petri disk. The result showed that by in vivo treatment, crude extract of Ampupu tree bark with concentration of 1.5%, 3,0% and 4.5%, were able to inhibit the fungal growth of Fusarium miniliforme as the causal agent of corncob rot with diameter of inhibition zone 0.18 mm, 1.85 mm and 2.01 mm, respectively. The higher the concentration of the crude extract given, the larger the diameter of inhibition zone formed.
Co-Authors Aloysius Rusae Blasius Atini Lukman Muhamad Masa Lusia Herlina Lawa Hale Mardit N Nalle Meitini Proborini Wahyuni Ni Putu Adriani Astiti Umbu Joka