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All Journal METAMORFOSA Journal of Biological Sciences Jurnal Biologi Udayana SIMBIOSIS Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Mikologi Indonesia
Meitini Proborini Wahyuni
Program Studi Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Udayana. Jl. Raya Kampus UNUD, Jimbaran, Kuta Selatan, 80361, Bali
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology

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Identifikasi Senyawa Aktif Ekstrak Daun Tembelekan (Lantana camaraL.) Yang Berpotensi Sebagai Pengendali JamurFusarium solani (Mart.) Sacc. Penyebab Layu Batang dan Busuk Akar Tanaman Pisang Kepok(Musa paradisiacaforma typica) Ni Made Dewi Wahyuni; Ni Putu Adriani Astiti; Meitini Wahyuni Proborini
Metamorfosa: Journal of Biological Sciences Vol 6 No 2 (2019)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2019.v06.i02.p08

Abstract

Banana plants have been used as parts of ritualism by Hindu’S people in Bali nevertheles these plants are often infected by fungal pathogens. Fusarium sp is one of the most common fungal pathogen infecting these plants. Commonly to release this pathogen the usage synthetic fungicides. To reduce the use of these synthetic fungicides, alternative method such as using natural compound of fungicides has been researched. The main objective of this research was to investigate the compounds of tembelekan leaf extract to control the growth of F. solani (Mart.) Sacc, the causative agent of stem wilt and root rot in kapok banana by using GC-MS. The result of identification using GC-MS identified that there are four compounds is 2,2,4-Trimethyl-1,3-pentanediol diisobutyrate, Hexadecanoic acid, Terephthali acid, and Squalene.
ISOLASI DAN IDENTIFIKASI BAKTERI PENYEBAB BUSUK LUNAK PADA UMBI WORTEL (Daucus carota L.) VARIETAS LOKAL DI BALI Ni Wayan Desi Bintari; Retno Kawuri; Meitini Wahyuni Proborini
Metamorfosa: Journal of Biological Sciences Vol 2 No 1 (2015)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

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Abstract

Soft rot bacteria infection in carrot tuber (D. carota L.) causes severe economic losses. Soft rot disease can be caused by various bacteria belonging to Enterobacteriaceae. This study aimed to isolate and identify bacteria as causal agent of soft rot disease in local carrot variety in Bali. Samples were collected at Badung Tradisional Market, Denpasar, Bali. Isolation was carried out by serial dilution method (Platting Method). Eight bacteria (BL1, BL2, BL3, BL4, BL5, BL6, BL7 and BL8) were isolated from soft rot tuber. BL6 isolate showed positive result in Postulat Koch test that caused soft rot on carrot tuber. The result of identification by Microgen™ GnA+B-ID System and identification book Bergeys’s Manual of Determinative Bacteriology Ninth Edition (Holt et al., 1994), BL6 was identified as Citrobacter.
Identifikasi Jamur Endofit pada Tanaman Anggur Bali (Vitis vinifera L. Var Alphonso lavalle) serta Potensi Antagonisnya terhadap Botrytis cinerea Pers. Penyebab Penyakit Busuk Kelabu Putu Anggan Pradipta Utama; Meitini Proborini Wahyuni; Ni Made Susun Parwanayoni
Metamorfosa: Journal of Biological Sciences Vol 8 No 2 (2021)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2021.v08.i02.p01

Abstract

This research aims to isolate and test the ability of endophytic fungi from the Bali Grape plant (Vitis vinifera L. var Alphonso lavalle) against botrytis cinerea pers. cause of gray rot in Grapes Bali. This research is an exploration study that is then continued with experimental. This research sample was isolated from Balinese grapes, namely healthy roots, stems, and leaves as well as diseased fruit parts from Balinese vineyards in Banyupoh Village, Gerokgak, Buleleng, Bali. Isolates of endophyte fungi that are successfully isolated are then tested using dual culture methods against pathogenic fungi. The test results of five isolates of endophytic fungi obtained three types of fungi that have the tasteless power of two types of fungi Trichoderma spp. (1) (2) and Mucor spp. with consecutive bonding power of 90.67 ± 16.17%, 75.67 ± 31.13%, and 82.67 ± 39.02. These results show that endophyte fungi have potential as biocontrol agents. Keywords : Mushroom endophyte, Botrytis cinerea Pers., Biocontrol
Eksplorasi Dan Identifikasi Mikroba Pada Rhizosfer Tanaman Stroberi (Fragaria x ananassa Dutch.) Di Kawasan Pancasari Bedugul Gustu Widi Putra; Yan Ramona; Meitini Wahyuni Proborini
Metamorfosa: Journal of Biological Sciences Vol 7 No 2 (2020)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2020.v07.i02.p09

Abstract

The main objective of this research was to isolate and identify microbes residing in the rhizosphere zone of strawberry (Fragaria x ananassa Dutch.), cultivated at Pancasari village, Bedugul Bali. This was an explorative and descriptive research conducted from April to July 2018. Soil samples were collected from the rhizosphere of strawberry plants and microbes residing in them were isolated by applying dilution and platting method, purified, and identified at the Food Microbology Laboratory, Faculty of Agricultural Technology, Udayana University. Important characteristics of the isolated microbes were compared to those specified in references, such as Bergey’s Manual of Determine Bacteriology 9th Edition (Holt et al., 1994) for bacterial isolates and Fungal Identification keys (Barnett and Hunter, 1972; dan Pitt and Hocking, 2009) for fungal isolates. Microbes successfully isolated and identified in this research included fungi and bacteria belong to Aspergillus flavus, Aspergillus niger, Fusarium oxysporum, and Colletotrichum sp., Pseudomonas sp., and Bacillus sp. Keywords: rhizosphere, strawberry, microbes.
Eksplorasi dan Identifikasi Jamur Pelarut Fosfat di Kawasan Hutan Taman Nasional Bali Barat (TNBB) Riswanda Dwiky Priyanta; Meitini Wahyuni Proborini; Anak Agung Raka Dalem
Metamorfosa: Journal of Biological Sciences Vol 6 No 1 (2019)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2019.v06.i01.p21

Abstract

Research on the exploration and identification of phosphate solvent fungi has never been carried out in West Bali National Park (TNBB), therefore researchers aims to explore and identify microscopic fungi to be used as phosphate solvent fungi which later will be taken from each plant soil samples (Lantana camara) that the presence is very common in TNBB. The research was implemented in two stages. The first stage is exploration of soil fungi in the field (TNBB) and identification of fungal species and the second stage is the phosphate solvent fungus test on Pikovskaya media. The results of the identification of the fungi obtained as follow: Aspergillus niger, Aspergillus bertholletius, Aspergillus flavus, Aspergillus isolate 4, Aspergillus isolate 5, Penicillium citrinum, and Trichoderma amazonicum. From the entire types of fungi obtained, there are onlybfour fungi that have the potential as phosphate solvents, namely Aspergillus niger, Aspergillus flavus, Aspergillus bertholletius and Penicillium citrinum with the presence of clear zones on Pikovskaya media. Fungi that has the best potential in the process of phosphate dissolution is Aspergillus niger. Key words: Rhizosfer, Lantana camara, clear zone, phosphate solvent fungus
Perubahan Golongan Darah Berdasarkan Pengaruh Waktu dan Mikroorganisme Yang Berperan Muhamad Masyrur; I Ketut Junitha; Meitini W. Proborini
Metamorfosa: Journal of Biological Sciences Vol 6 No 2 (2019)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2019.v06.i02.p05

Abstract

Blood is one of the most important evidence in the forensic analysis. Blood has a special characteristic or identity of the owner. Blood can be analyzed blood type to know the owner of the blood. But in the course of time, the blood will be degraded so it will be change of blood type into blood type O. One of the contributing factors is the presence of microorganisms that it grow in the blood. The material of this research is the human blood of blood type A, B, AB and O from 4 (four) respondents of 3 (three) males and 1 (one) female who are 20 to 40 years old. Each sampel of 150 µL was dropped on sterile gauze size 2 x 6 cm, then it was dried. Sample were stored at room temperature (± 30 oC) for 0, 30, 60, 90 and 120 days. Sampel was tested for blood type using absorption elution method based on the work instructions from The Police Forensic Laboratory of Denpasar Branch in 2016. The results of this research indicate that the human dried blood stored on sterile gauze media for 30 and 60 days has not changed its blood type. Storage of the human dried blood for 90 and 120 days, it have changed blood type with found antigen A or B that was not detected back through absorbtion elution analysis on blood group A, B and AB. This research found 9 species of fungi and 7 species of bacteria that they grow on the blood samples. Species of Fungi and bacteria that have high ability to degrading all blood group (A, B and AB) are Acromonium carticola, Alternaria alternata, Aspergillus flavus, Aspergillus niger, Penicillium sp and Bacillus coagulans. While species of fungi and bacteria that have high ability to degrading blood group B or AB are Fusarium sp, Trichoderma harzianum, Trichoderma viridae, Bacillus sp and Staphylococcus cohnii.
Hambatan in Vitro Cendawan Antagonis pada Fusarium sp., Penyebab Penyakit pada Tanaman Buah Naga (Hylocereus undatus (Haw.) Britton & Rose) Dewa Ayu Andriastini; Yan Ramona; Meitini Wahyuni Proborini
Metamorfosa: Journal of Biological Sciences Vol 5 No 2 (2018)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2018.v05.i02.p14

Abstract

A research on in vitro inhibition of fungal antagonists, isolated from dragon fruit plantation in Sembung village, Bali, on Fusarium sp. (the disease causative agent of dragon fruit plant) was conducted with the main objective to investigate the effectiveness of these fungal antagonists to inhibit the in vitro growth of the pathogen. Dual assay method was applied in this experiment. The results showed that three potential fungal antagonists were successfully isolated in this research and they were identified as Trichoderma harzianum, Aspergillus niger, dan Paecilomyces lilacinus. All these fungal antagonists showed antagonistic activity against Fusarium sp. which was statistically significant (p<0.05) when compared to control. This indicated that all antagonist isolates were potential to be developed as biocontrol agent candidates.
IDENTIFIKASI ANTAGONIS DARI Xanthomonas campestris YANG DIISOLASI DARI RHIZOSPHERE PERKEBUNAN BROKOLI (Brassica oleracea var. italica) DI DESA KEMBANG MERTA, KABUPATEN TABANAN, BALI Nadya Treesna Wulansari; Yan Ramona; Meitini Wahyuni Proborini
Metamorfosa: Journal of Biological Sciences Vol 2 No 1 (2015)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

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Abstract

The main objectives of this research were to isolate and identify antagonists of Xanthomonas campestris from rhizosphere zone of broccoli plants. Soil samples were collected from broccoli farm located at Kembang Merta village, Tabanan, Bali. Isolation and identification of the antagonists were conducted at the Laboratory of Microbiology, Udayana University. Two fungal (Trichoderma harzianum and  Trichoderma viride) and two bacterial (Bacillus sp. and Pseudomonas sp.) antagonists potentially to be developed as biocontrol agents of Xanthomonas campestris were successfully identified in this research.
Aplikasi Spora Endomikoriza, Kompos Dan Trichoderma Spp. Meningkatkan Pertumbuhan Bibit Kakao (Theobroma cacao L.) Tadzkia Hanifah Akbar; Meitini Wahyuni Proborini; Made Ria Defiani
Metamorfosa: Journal of Biological Sciences Vol 9 No 1 (2022)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2022.v09.i01.p05

Abstract

Pembibitan kakao (Theobroma cacao L.) merupakan salah satu upaya untuk mempertahankan agroforestri dan menjaga fungsi hutan. Penelitian bertujuan menganalisis kombinasi antara jamur endomikoriza, kompos, dan Trichoderma spp. terhadap pertumbuhan bibit kakao serta menentukan konsentrasi yang terbaik untuk meningkatkan pertumbuhan bibit kakao. Penelitian dilakukan di laboratorium taksonomi tumbuhan (mikologi) dan green house Persemaian Permanen Suwung, Badan Pengelolaan Daerah Aliran Sungai dan Hutan Lindung Hutan Anyar. Perlakuan disusun secara RAK (Rancangan Acak Kelompok) dengan 5 perlakuan yang terdiri dari 5 ulangan dan 3 unit tanaman untuk setiap ulangan. Perlakuan pertama menggunakan tanah steril (kontrol negatif), perlakuan kedua dengan tanah steril + kompos 10 g + Trichoderma spp 10 mL (kontrol positif), perlakuan ketiga dengan tanah steril dan inokulasi endomikoriza Glomus sp 100 butir + kompos 10 gram + Trichoderma spp. 10 mL, perlakuan keempat dengan tanah steril dan inokulasi endomikoriza Glomus sp 150 butir + kompos 10 g + Trichoderma spp. 10 mL, dan perlakuan kelima dengan tanah steril dan inokulasi endomikoriza Glomus sp 200 butir + kompos 10 g+ Trichoderma spp. 10 mL. Hasil penelitian menunjukkan bahwa perlakuan kelima (200 spora endomikoriza, 10 mL Trichoderma spp., dan 10 g kompos) merupakan kombinasi terbaik untuk pertumbuhan bibit kakao dilihat dari nilai parameter tinggi tanaman sebesar 29,03 cm, berat kering tanaman 3,55 g, berat kering akar 1,83 g, dan persentase kolonisasi endomikoriza sebesar 6,33 %. Kata kunci: Green house, Glomus sp., agroforestri, persentase kolonisasi
ISOLASI DAN IDENTIFIKASI BAKTERI PENYEBAB PENYAKIT BUSUK LUNAK PADA BUAH STROBERI (Fragaria x ananassa) Made Mega Yuliasari; Retno Kawuri; Meitini Wahyuni Proborini
Metamorfosa: Journal of Biological Sciences Vol 2 No 1 (2015)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

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Abstract

Soft rot on strawberry fruit was found in strawberry (F. x ananassa) plantation in Candi Kuning, Bedugul, Bali. Soft rot on strawberry fruit can be caused by microorganism i.e. bacteria. Objectives of the research were to isolate pathogen causing soft rot on strawberry fruit with plating method and to identify bacteria causing soft rot by using Kit MicrogenTM GNA+B-ID System and Bergey’s Manual of Determinative Bacteriology reference (Holt et al., 1994). Results showed there were five isolates of bacteria (IB-1, IB-2, IB-3, IB-4, and IB-5). Positive result of Postulat Koch showed that bacteria causing soft rot on strawberry is IB-1. Identification that was done by using Kit MicrogenTM GNA+B-ID System and Bergey’s Manual of Determinative Bacteriology reference (Holt et al., 1994), showed that the isolate IB-1 is Weeksella
Co-Authors Anak Agung Devina Asana Putri Anak Agung Gde Raka Dalem Anak Agung Ngurah Nara-Kusuma Anak Agung Yulia Anggiani Bekti Revi Rahayu Bernadina Metboki Deny Suhermawan Yusup Dewa Ayu Andriastini Dewa Ngurah Suprapta ENIEK KRISWIYANTI Gustu Widi Putra I Gede Sugita Artana I Ketut Junitha I KETUT MUKSIN I Ketut Sundra I MADE ADNYANA IDA AYU ASTARINI Ida Ayu Putu Suryanti Ida Bagus Gede Darmayasa Ida Bagus Gede Daymayasa Inna Narayani Irsan Nuhantoro Job Nico Subagio Junita Hardini Krisdayani, Putu Mita Lila, Kadek Dian Lila Sawitri Kumala Lilis Riana Tambunan Lily Windasari Made Mega Yuliasari Made Ria Defiani MADE SUSUN PARWANAYONI Maghfirotun Nisa Muhamad Masyrur Nadya Treesna Wulansari Naomi Anggi Triarta Ni Luh Desy Wulandari Ni Luh Suriani Ni Made Dewi Wahyuni Ni Putu Adriani Astiti Ni Putu Muni Raisani Ni Wayan Desi Bintari Pinki Prahara Taurisia Putu Anggan Pradipta Utama Putu Ayu Sukmawati Putu Shinta Devi Retno Kawuri Risha Masfufah Riswanda Dwiky Priyanta Sukmawati Sukmawati Sukmawati Sukmawati Tadzkia Hanifah Akbar Wijayanti, Febri Eka Wira Purnama Santi Yan Ramona