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All Journal International Journal of Biosciences and Biotechnology METAMORFOSA Journal of Biological Sciences Jurnal Mikologi Indonesia Metamorfosa: Journal of Biological Science Open Access DRIVERset
Junita Hardini
Program Studi Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Udayana. Jl. Raya Kampus UNUD, Jimbaran, Kuta Selatan, 80361, Bali, Indonesia.
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology

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DIVERSITY OF SPECIES CRUSTOSE LICHEN OF Plumeria spp. IN BALI ISLAND Junita Hardini; Rina Sri Kasiamdari; Santosa Santosa; Purnomo Purnomo
Metamorfosa: Journal of Biological Sciences Vol 5 No 1 (2018)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2018.v05.i01.p19

Abstract

The research of species crustose lichen was conducted in the lowlands of the Bali Island and was conducted randomly at a height of 0-500 m above sea level. The aim is to find out the diversity of species crustose lichen living in Plumeria spp. The method used is descriptive qualitative analysis method. Data collection was done by field observation and specimen collection. The lichen specimens were observed and identified morphologically, anatomically, and chemically. In this study found of four families consisting of six genera and 15 species, namely Graphina sp., Phaeographina sp., Graphis sp., Graphis immersella, G. nilgiriensis, G. modesta, G. nana, and G. conferta (Graphidaceae); Lecanora sp.1, and Lecanora sp.2 (Lecanoraceae); Lepraria sp. (Stereocaulaceae); Caloplaca sp. (Teloschistaceae). The most common species is Graphis sp. (88%).
Keanekaragaman Jenis Tumbuhan Bahan Ramuan “Boreh Basanbuat” Untuk Memperlancar Produksi Air Susu Ibu (ASI) di Bali Enik Kriswiyanti; Ni Nyoman Darsini; Junita Hardini; Ni Putu Ariwathi
Metamorfosa: Journal of Biological Sciences Vol 8 No 2 (2021)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2021.v08.i02.p15

Abstract

Boreh (bahasa Bali) merupakan salah satu bentuk ramuan tradisional berupa campuran rempah-rempah yang dihaluskan, ditambah cairan (air/arak/minyak kelapa) kemudian dilulurkan pada bagian tubuh. Salah satu boreh yang dapat digunakan untuk memperlancar produksi air susu ibu (ASI) adalah boreh basanbuat. Penelitian ini bertujuan untuk mengetahui jenis dan bagian tumbuhan yang dapat digunakan untuk membuat boreh basanbuat di Bali. Metode penelitian yang digunakan: purposive sampling, pengumpulan data dengan cara wawancara, observasi dan dokumentasi dengan pedagang crakenan, pengobat tradisional (battra) dan masyarakat dari kota dan kabupaten wilayah propinsi Bali, sebanyak 90 responden. Hasil penelitian didapatkan 54 jenis tumbuhan (52 genus, 29 familia) untuk membuat 64 jenis ramuan, jenis tumbuhan yang paling banyak digunakan dari anggota famili Zingiberaceae, bagian tumbuhan yang paling banyak digunakan adalah daun (23,21%). Ramuan boreh basanbuat umumnya menggunakan 5-6 jenis tumbuhan, yaitu menggunakan: beras (Oryza sativa L.), biji kelabet (Trigonella feonum-graecum L.), akar wangi (Androphogon zizanioides (L) Urban), kayu/bubuk cendana (Santalum album L.), daun delem/ nilam (Pogostemon cablin (Blanco) Benth.) dan rimpang kencur (Kaemferia galanga L). Manfaat bahan boreh selain memperlancar produksi ASI, juga untuk aroma terapi, antiinflamasi, anti pembengkakan, antibakteri, antijamur, dan lain sebagainya.
Tumbuhan Pewarna Alami Dan Pengolahannya Pada Tenun Ikat Amarasi Di Desa Tunbaun, Kecamatan Amarasi Barat, Kabupaten Kupang, Nusa Tenggara Timur Fransiska Nitti; Junita Hardini; Made Pharmawati
Metamorfosa: Journal of Biological Sciences Vol 9 No 1 (2022)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2022.v09.i01.p17

Abstract

This study aims to identify the types of plants, its parts that are used and the processing methods for amarasi weaving fabrics. Data collection in this study were carried out in of the village of Tunbaun, West Amarasi Sub-district, Kupang District, East Nusa Tenggara as one of the centers for the production of amarasi woven fabrics. Qualitative method was used in this study with interview, observation, documentation and literature study techniques. The results showed that the weaving craftsmen in Tunbaun village, used four types of plants as natural dye for weaving including noni root bark (Indigofera tinctoria L., Fabaceae), indigo leaves (Indigofera tinctoria L., Fabaceae), young teak leaves (Tectona grandis L.f., Lamiaceae) and turmeric rhizome (Curcuma domestica Val., Zingiberaceae). Processing of ground noni root bark, then boiled in water to get a red color. Indigo leaves are soaked for 2x24 hours, added lime and let with solution and allowed to stand for 24 to obtain a blue indigo paste. Young teak leaves are boiled in water until they get a purple color. While the turmeric rhizome is grated and then boiled in water to get a yellow color. Keywords: Amarasi, Natural Dyes, Weaving
Potential of Awar-awar Leaf Methanol Extract (Ficus septica Burm. f.) Against Aspergillus niger on Balinese Lontar Ni Komang Deny Julyeda; Ida Bagus Gde Darmayasa; Junita Hardini
Metamorfosa: Journal of Biological Sciences Vol 8 No 2 (2021)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2021.v08.i02.p10

Abstract

Ficus septica Burm. f. is a wild plant that has antifungal compounds. Exploration of antifungal compounds from natural material important in inhibiting the growth of Aspergillus niger, one of Balinese lontar destructive fungus. Natural material use to reduse lontar damage due to synthetic materials. This study aims to inhibition of awar-awar leaf methanol extract (in vitro). The method used was a complete randomized design with 7 treatments for the concentration of the awar-awar leaf methanolic extract of 10.000, 20.000, 30.000, 40.000, 50.000 ppm (b/v), positive control (nystatin), and negative control (methanol). The minimum inhibitory concentration (MIC) test uses 6 treatments for the concentration of the awar-awar leaf methanolic extract of 100, 500, 1000, 2000 ppm (w/v), positive control (nystatin), and negative control (methanol). Giving the extract to the diffusion well with potato dextrose agar (PDA) media. The research data were analyzed statistically ANOVA (DMRT with 5% significance level) with the SPSS 23 version for windows. The results showed that the awar-awar leaf methanol extract had MIC at a concentration of 500 ppm (w/v). Concentrations of 10.000-50.000 ppm (w/v) indicate the inhibition zone diameter of 20.50 mm, respectively; 23.00 mm; 23.50 mm; 23.75 mm; 24.75 mm. The concentration of 10.000 ppm (w/v) has higher inhibition than the positive control. The concentration of 20.000 ppm (w/v) was the optimum concentration of inhibition of awar-awar leaf methanol extract. Conclusion of this research was awar-awar leaf methanol extract had anti-fungal compounds which inhibited the growth of A. niger. This extract can be used as an alternative to inhibit the growth of fungi that cause lontar damage. Keyword: Aspergillus niger, destructive fungi, Ficus septica Burm. f.
Peranan FMA Glomus sp. dan Pupuk Anorganik terhadap Produktivitas Tanaman Kedelai (Glycine max (L.) Merrill) var. Lokal Bali Naomi Anggi Triarta; Meitini Wahyuni Proborini; Junita Hardini
Jurnal Mikologi Indonesia Vol 3, No 2 (2019): Desember 2019
Publisher : Perhimpunan Mikologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (259.158 KB) | DOI: 10.46638/jmi.v3i2.60

Abstract

Produktivitas tanaman kedelai (Glycine max var. Lokal Bali) dapat ditingkatkan melalui pemupukan. Salah satu pupuk hayati yang bisa diaplikasi sebagai pendamping pupuk anorganik adalah fungi mikoriza arbuskula (FMA). Penelitian ini bertujuan untuk mengetahui peranan FMA Glomus sp. dan pupuk NK terhadap produktivitas tanaman kedelai lokal Bali. Penelitian ini dilakukan di laboratorium Taksonomi Tumbuhan/Mikologi dan Shading house Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana. Penelitian ini menggunakan Racangan Acak Kelompok (RAK) dengan 5 perlakuan yaitu (A) Tanah steril (kontrol) atau tanpa inokulasi FAM dan pupuk NK; (B) Tanah steril, 50 spora Glomus sp.; (C) Tanah steril, 50 spora Glomus sp. dan pupuk NK 5 g; (D) Tanah steril, 50 spora Glomus sp. dan pupuk NK 10 g; (E) Tanah steril, 50 spora Glomus sp. dan pupuk NK 15 g. Variabel yang diamati adalah tinggi tanaman, berat kering tajuk, berat kering akar, jumlah polong isi dan polong hampa, berat basah dan berat kering polong, dan persentase kolonisasi FMA Glomus sp. Hasil penelitian menunjukkan bahwa pemberian 50 butir spora Glomus sp. dan pupuk NK 5 g pada perlakuan C berperan meningkatkan produktivitas tanaman kedelai terlihat pada jumlah polong isi sebanyak 51 polong per tanaman yang berbeda nyata secara statistik dan memiliki berat kering sebesar 9,10 g, tetapi tidak memberikan pengaruh yang berbeda nyata terhadap pertumuhan tanaman kedelai.
Callus Induction In Leucaena (Leucaena leucocephala (Lam.) de Wit) As An Effort To Provide Target Transformation Through Agrobacterium tumefaciens Ni Nyoman Nila Arieswari; Ida Ayu Astarini; Junita Hardini; Austin Ryan Garrido; Debora Margareth; Jennifer Crismonika; Sebastian S. Cocioba
International Journal of Biosciences and Biotechnology Vol 10 No 1 (2022): INTERNATIONAL JOURNAL OF BIOSCIENCES AND BIOTECHNOLOGY
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2022.v10.i01.p02

Abstract

Leucaena is a plant that produces biomass productivity in the form of hardwood for fuel with low humidity and high calorific value. However, Leucaena is also classified as an invasive plant which can cause the urgency of native plant species and ecosystems in Indonesia. Therefore, the formation of sterile Leucaena needs to be done, one of which is through genetic transformation using Agrobacterium tumefaciens. Callus is used as a target for transformants in the genetic transformation process, so it is necessary to use appropriate media and PGR. This study aimed to determine the type of media and the concentration of 2,4-D on callus induction. This research is an experimental study with a completely randomized design (CRD) method with two factors. The first factor is the type of media (MS and WPM) and the second factor is the concentration of 2,4-D (0; 0.25; 0.50; 0.75; 1.00; 1.25 and 1.50 mgL-1 ). Each treatment was repeated three times so that 42 experimental units were obtained. Parameters observed were callus initiation, callus fresh weight (gram), callus texture and color. Quantitative data is analyzed by analysis of variance (ANOVA). The results showed that the use of media had a significant effect (P<0.05) on callus fresh weight. The use of 2,4- D concentration had a significant effect (P>0.05) on callus texture. The use of WPM media resulted in the fastest callus emergence time (6.67±0.57), the best callus texture (crumb callus type 2) and the best callus color (green). Meanwhile, the highest fresh weight (2,48±0.83) was in the use of MS media. The fastest callus emergence time occurred in the control (without the addition of 2,4-D) (7.33±0.57 and 6.67±0.57), the highest average fresh callus weight (2,48±0.83 and 2.35±0.32) occurred in the treatment with the addition of 1.00 mgL-1 2,4-D with a crumb callus texture of type 2 and callus green color only appeared in the treatment with a concentration of 0.25 mgL-1.
Identifikasi dan Skrining Fitokimia Jamur Endofit pada Mangrove Sonneratia alba J.E. Smith Anak Agung Putri Suci Hati; Fainmarinat Selviani Inabuy; Junita Hardini
Metamorfosa: Journal of Biological Sciences Vol. 11 No. 01 (2024)
Publisher : Program Magister Ilmu Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2024.v11.i01.p8

Abstract

Jamur endofit dikenal sebagai sumber alternatif senyawa bioaktif tumbuhan. Tumbuhan mangrove Sonneratia alba J.E. Smith diketahui berperan sebagai inang bagi jamur endofit. Penelitian ini bertujuan mengidentifikasi jenis jamur endofit pada tumbuhan S. alba dan skrining kelompok senyawa metabolit sekunder yang dihasilkan oleh jamur endofit. Sampel diperoleh di Taman Hutan Raya Ngurah Rai, Pamogan, Denpasar Selatan. Pengambilan sampel berupa daun, buah, dan kulit batang tumbuhan yang sehat. Organ daun, buah, dan kulit batang S. alba diisolasi secara in vitro pada media Potato Dextrose Agar (PDA). Isolat murni jamur endofit dimurnikan menurut karakteristik morfologi kemudian ditumbuhkan pada media beras. Isolat murni diidentifikasi dan dilakukan skrining fitokimia secara kolorimetri, meliputi uji alkaloid, flavonoid, dan terpenoid. Dari tujuh isolat jamur endofit diperoleh pada penelitian ini, lima isolat telah teridentifikasi yaitu Colletotrichum dan Phyllosticta dari daun. Penicillium dan Neopestalotiopsis sonneratae dari buah, dan Trichoderma harzianum dari kulit batang. Dua isolat endofit lainnya, yakni dari daun dan kulit batang belum teridentifikasi. Ketujuh isolat menghasilkan metabolit sekunder golongan alkaloid dan terpenoid.  
Identifikasi dan Skrining Fitokimia Jamur Endofit pada Mangrove Sonneratia alba J.E. Smith Anak Agung Putri Suci Hati; Fainmarinat Selviani Inabuy; Junita Hardini
Metamorfosa: Journal of Biological Sciences Vol. 11 No. 01 (2024)
Publisher : Program Magister Ilmu Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2024.v11.i01.p8

Abstract

Jamur endofit dikenal sebagai sumber alternatif senyawa bioaktif tumbuhan. Tumbuhan mangrove Sonneratia alba J.E. Smith diketahui berperan sebagai inang bagi jamur endofit. Penelitian ini bertujuan mengidentifikasi jenis jamur endofit pada tumbuhan S. alba dan skrining kelompok senyawa metabolit sekunder yang dihasilkan oleh jamur endofit. Sampel diperoleh di Taman Hutan Raya Ngurah Rai, Pamogan, Denpasar Selatan. Pengambilan sampel berupa daun, buah, dan kulit batang tumbuhan yang sehat. Organ daun, buah, dan kulit batang S. alba diisolasi secara in vitro pada media Potato Dextrose Agar (PDA). Isolat murni jamur endofit dimurnikan menurut karakteristik morfologi kemudian ditumbuhkan pada media beras. Isolat murni diidentifikasi dan dilakukan skrining fitokimia secara kolorimetri, meliputi uji alkaloid, flavonoid, dan terpenoid. Dari tujuh isolat jamur endofit diperoleh pada penelitian ini, lima isolat telah teridentifikasi yaitu Colletotrichum dan Phyllosticta dari daun. Penicillium dan Neopestalotiopsis sonneratae dari buah, dan Trichoderma harzianum dari kulit batang. Dua isolat endofit lainnya, yakni dari daun dan kulit batang belum teridentifikasi. Ketujuh isolat menghasilkan metabolit sekunder golongan alkaloid dan terpenoid.  
Co-Authors Anak Agung Putri Suci Hati Austin Ryan Garrido Candra Dewi Oktaviawati Debora Margareth Deny Christine Sidabutar ENIEK KRISWIYANTI Fainmarinat Selviani Inabuy Fransiska Nitti IDA AYU ASTARINI Ida Bagus Gede Darmayasa Jennifer Crismonika MADE PHARMAWATI Meitini Proborini Wahyuni Naomi Anggi Triarta Ni Komang Deny Julyeda NI NYOMAN DARSINI Ni Nyoman Nila Arieswari Ni Putu Ariwathi Nyoman Mega Antari Purnomo Purnomo Rina Sri Kasiamdari SANG KETUT SUDIRGA Santosa Santosa Sebastian S. Cocioba