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All Journal Medicina E-Jurnal Medika Udayana Jurnal Veteriner Health Science Journal of Indonesia The Indonesian Biomedical Journal
Dewa Ayu Agus Sri Laksmi
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Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Medicine & Pharmacology Nursing Public Health Veterinary

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Journal : Jurnal Veteriner

 1 
Mosquito-specific viruses (family Flaviviridae, genus Flavivirus) Diisolasi pada Nyamuk Anopheles vagus di Bali Putu Ayu Asri Damayanti; I Nyoman Mantik Astawa; Anak Agung Ayu Mirah Adi; I Made Sudarmaja; I Kadek Swastika; Dewa Ayu Agus Sri Laksemi; Ni Luh Putu Eka Diarthini
Jurnal Veteriner Vol 22 No 2 (2021)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (203.933 KB) | DOI: 10.19087/jveteriner.2021.22.2.189

Abstract

Mosquito-specific viruses (MSVs) adalah virus yang hanya dapat bereplikasi pada sel nyamuk. Virus ini terdiri dari berbagai genus, salah satunya yang paling banyak ditemukan adalah dari famili Flaviviridae, genus Flavivirus. Namun, data keberadaan dan karakteristik MSVs dan vektornya di Bali saat ini sangat terbatas. Oleh karena itu, pengamatan untuk memperluas penemuan keragaman vektor dan filogenetik MSVs famili Flaviviridae, genus Flavivirus di Bali dilakukan pada tahun 2016-2018. Nyamuk dewasa ditangkap menggunakan light trap dan dikelompokkan berdasarkan spesies. Isolasi dan propagasi virus dilakukan pada galur sel C6/36 dan baby hamster kidney-21 (BHK-21). Identifikasi virus dilakukan dengan menggunakan one step reverse-transcriptase polymerase chain reaction (RT-PCR). Terdapat dua pool yang berasal dari nyamuk Anopheles vagus menampakan cythopathic effect (CPE) hanya pada galur sel C6/36 dari total 158 pool. Virus yang diisolasi memiliki persentase identity sekuen nukleotida tertinggi 97% dan sekuen asam amino 96% dengan virus Culex theileri Flavivirus isolat JKT-8650 yang diisolasi pada tahun 1981. Selanjutnya, virus dinamakan Mosquito Flavivirus Isolate Bali (MFB) dengan accession numbers KY995166 dan KY290258. Analisis filogenetik menunjukan bahwa MFB berada satu kluster dengan Culex theileri Flavivirus (CTFV) dari Indonesia, Culex Flavivuruses-Myanmar, Culex theileri Flavivirus-Portugal, dan Mosquito Flavivirus-Turki. Terdapat delapan nukelotida dan enam asam amino yang berbeda antara MFB dan CTFV Indonesia. Pada penelitian ini dapat disimpulkan bahwa MSVs dari famili Flaviviridae, genus Flavivirus berhasil diisolasi dari nyamuk An. vagus di Bali.
Seroprevalensi yang Tinggi dan Faktor-Faktor Risiko Toksoplasmosis pada Darah Donor dan Wanita di Bali (HIGH SEROPREVALENCE AND RISK FACTORS OF TOKSOPLASMOSIS AMONG BLOOD DONORS AND WOMEN IN BALI) Dewa Ayu Agus Sri Laksemi; Wayan Tunas Artama; Mahardika Agus Wijayanti
Jurnal Veteriner Vol 14 No 2 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (124.775 KB)

Abstract

Toxoplasmosis is an important public health problem because of its worldwide distribution, economicand social impact due to high sequele that may cause such as mental retardation and blindness in children.The aims of this study were to asses serological prevalence of toxoplasmosis in donors and women in Baliand get an overview of association between risk factors and toxoplasmosis infection, i.e.: comprising catownership, food pattern, occupation related to contact with raw meat and activities related to contact withsoil. Serum samples were collected from donors consecutively, while simple cluster design was used forsampling woman. Data on demographics and risk factors for toxoplasmosis were obtained usingquestionnaire. Serological prevalence of toxoplasmosis in donors was 35,9%, while in women was 63.9%.Serological prevalence of toxoplasmosis  in donors at District Badung was 29,2%, Tabanan 36.8%, Gianyar25.0%, Denpasar 41.1%, Klungkung 25.0%, and Bangli 8.3%. Serological prevalence of toxoplasmosis  inwomen at District Badung was 33.3%, Tabanan 66.5%, Gianyar 82.5%, Denpasar 71.1%, Klungkung 81.5%and Bangli 16.7%. Risk factor that play a role in toxoplasmosis infection were food pattern and occupationrelated to contact with soil. The seroprevalence of toxoplasmosis in voluntary blood donors and child-bearing age is relatively high due to local habbit of Balinese society that consume raw meat called lawarand sate
Penurunan Kadar Trigliserida pada Tikus Jantan dengan Terapi Growth Hormone (DECREASE OF TRIGLYCERIDE LEVEL IN MALE RAT BY GROWTH HORMONE TREATMENT) I Gusti Ayu Dewi Ratnayanti; I Wayan Sugiritama; Ida Ayu Ika Wahyuniari; Ni Made Linawati; I Gusti Ngurah Mayun; Dewa Ayu Agus Sri Laksemi; I Gusti Ngurah Sri Wiryawan; I Gusti Kamasan Nyoman Arijana
Jurnal Veteriner Vol 14 No 2 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (143.93 KB)

Abstract

The use of growth hormone (GH) as cardiovascular disease treatment is still controversial. In thispreliminary study the effect of growth hormone therapy on plasma triglyceride level in dyslipidemia wasexamined. Pre and post control group design study was done using 20 dyslipidemic (total cholesterol >200mg/dL) male rats, age 11–12 month-old. The subjects were divided into four groups, aquadest (P0), GH0.02 IU/day (P1), GH 0.04 IU/day (P2), and GH 0.08 IU/day (P3). All subjects were given high cholesteroldiet for three weeks to achieve dyslipidemic in blood level. Aquadest and GH were injected subcutaneouslyonce daily for two weeks. Triglyceride plasma level was measured on day 22nd and 38th by using colorimetricenzymatic test. The mean of pre test plasma triglyceride level of all groups was 136.30 mg/dL and nosignificant difference was found among the groups (p > 0.05). Growth hormone therapy significantly reducedplasma triglyceride level of P1 by 11.78% (118.82 mg/dL, p < 0.01), P2 by 23.46% (103.41 mg/dL, p < 0.01),and P3 by 35.15% (90.22 mg/dL, p < 0.01). Comparison of  post test data amomg the groups showedsignificant difference (p < 0.01). This study show that growth hormone therapy could reduce plasmatriglyceride level in dyslipidemic rat. However, further research is needed to more understand the effect ofthe therapy on cardiovascular diseases.
Co-Authors Anak Agung Ayu Mirah Adi I Gede Gita Sastrawan I Gusti Ayu Dewi Ratnayanti I Gusti Kamasan Arijana I Gusti Ngurah Mayun I Gusti Nyoman Sri Wiryawan I Kadek Swastika I Ketut Tunas I Made Sudarmaja I NYOMAN MANTIK ASTAWA I Wayan Sugiritama Ida Ayu Dewi Wiryantini Jordaniel Setiabudi Made Indira Dianti Sanjiwani Mahardika Agus Wijayanti Ni Komang Indriyani Ni Luh Ariwati Ni Luh Gede Dian Ratna Dewi Ni Luh Putu Eka Diarthini Ni Made Linawati Ni Putu Sanjiwani Ni Putu Wardani Putu Ayu Asri Damayanti Wayan Tunas Artama