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I MADE WINANTARA
Dinas Pertanian dan Pangan Kabupaten Badung
Agriculture, Biological Sciences & Forestry

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Isolasi dan Seleksi Bakteri Antagonis Sebagai Alternatif Pengendalian Penyakit Layu Stroberi I GUSTI NGURAH PRABU WIRA SANJAYA; GUSTI NGURAH ALIT SUSANTA WIRYA; TRISNA AGUNG PHABIOLA; I MADE WINANTARA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.8, No.2, April 2019
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Isolation and Selection of Antagonis Bacteria as an Alternative Control of Strawberry Wilt Diseases Strawberry (Fragaria sp.) is a plant that can produce fruit and a members of Rosaceae familia that entered Indonesia in the 1980s and began cultivativated in Candi Kuning Village, Bali at 1983. The farmer reported in 2017 at Candi Kuning Village, Bali, there are many strawberries plants are exposed to wilting disease caused by Fusarium oxysporum which can decreased strawberry production up to 80%. The purpose of this study is to find alternatives ways of chemical control that can cause environmental damage. Biological control, especially the use of antagonistic bacteria, has potential as a replacement because it is much more environmentally friendly and can create sustainable agriculture. The method used are isolation and selection, in vitro inhibitory test, and hypersensitive test on tobacco plants. Research activities included (1) sampling, (2) Fusarium oxysporum isolation from symptomatic strawberry plants, (3) isolation and selection of antagonistic bacterial candidates, (4) antagonistic candidate bacteria inhibitory test against Fusarium oxysporum by dual cultur method in vitro, and (5) hypersensitive test of antagonistic bacterial candidates on tobacco plant leaves. The result of this research is Fusarium oxysporum successfully isolated, obtained 24 candidates of antagonistic bacteria and only 4 candidates of antagonistic bacterial isolates capable of well inhibiting the development of Fusarium oxysporum with the average inhibitory percentage are more than 60 % which is the best inhibitory percentage of 90.17 % and in the hypersensitive test, the bacterial candidates tested were non-pathogenic to the plant.
Metode Grafting Tanaman Tomat Menggunakan Batang Bawah Terong Untuk Mengatasi Genangan dan Mengendalikan Penyakit Tular Tanah di Kebun Percobaan Fakultas Pertanian Universitas Udayana I PUTU SUDIARTA; GUSTI NGURAH ALIT SUSANTA WIRYA; I MADE WINANTARA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.8, No.1, Januari 2019
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Grafting Method of Tomato using rootstock of eggplant to minimize problems caused by flooding and soil-borne diseases in Faculty of Agriculture Research Field Udayana University The study was conducted to determine the effectiveness of the Grafting Method of Tomato using rootstock of eggplant to minimize problems caused by flooding and soilborne diseases in Faculty of Agriculture Research Field Udayana University. The experimental design was used a Randomize bloc design (RBD), with 3 treatments including control and 9 replications. The treatments are, grafting, non-grafting and control usually use by the farmers. The first step of the research is grafting or tomato as a scion with eggplant as a rootstock. The grafting method was used is base one the AVRDC-method. The result showed the grafted tomato was free from wilting disease on the other hand the non-grafted tomato showed the symptom of wild diseases, the data indicated the grafted tomato was resistant by the soil-borne diseases including bacteria wilt as well as Fusarium wilt disease. For flooding we cannot compare the significantly result between grafted tomato and non-grafted tomato because the season is not wet season. The suggestion of this research is recommended to doing the research in wet season for more clear understand the effect of grafted tomato to the flooding.
Isolasi dan Seleksi Bakteri Antagonis untuk Pengendalian Penyakit Busuk Batang Panili (Vanilla planifolia Andrews) Secara In Vitro I MADE ARIMBAWA; GUSTI NGURAH ALIT SUSANTA WIRYA; I MADE SUDANA; I MADE WINANTARA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.8, No.2, April 2019
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

Isolation and Selection of Antagonistic Bacteria to Control Stem Rot Disease on Vanilla Plant (Vanilla planifolia Andrews) by In Vitro Test. Isolation, selection, and identification of effective microbes are important steps to obtain biological agents. The perpose of this research was to get potential bacteria as controlling agents for stem rot of vanilla. The research was conducted at Plant Disease Laboratory, Faculty of Agriculture, Udayana University, Denpasar, from December 2017 to February 2018. The research comprised of isolation and identification the pathogen of stem rot of vanilla, pathogenicity test, isolation and identification of antagonistic bacteria, in vitro test of antagonistic bacteria ability to controled the pathogen of stem rot of vanilla, and hypersensitive response of antagonistic bacteria on tobacco plant. The result showed that the pathogen of stem rot on vanilla plants was identified as Fusarium oxysporum. There of 21 isolates of bacteria that has been isolated from field, there were 7 isolates could suppress of F. oxysporum growth are isolates P001, P002, P003, P004, P005, BM02, and BM03. In vitro test of antagonistic bacterial with F. oxysporum showed that isolate P002 could suppress pathogen growth with the highest percentage (98,36%), P005 (98,34%), P001 (92,78%), P003 (82,02%), P004 (80,34%), BM02 (71,80%), and BM03 (66,70%). Hypersensitive respons test showed that isolates P001, P002, P003, P004, P005, BM02, and BM03 weren’t pathogenic for plants.
Co-Authors Gusti Ngurah Alit Susanta Wirya I GUSTI NGURAH PRABU WIRA SANJAYA I Made Arimbawa I MADE SUDANA I Putu Sudiarta TRISNA AGUNG PHABIOLA