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I MADE SUDANA
Program Studi Agroekoteknologi, Fakultas Pertanian Universitas Udayana
Agriculture, Biological Sciences & Forestry

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Aplikasi Agens Hayati dan Humus untuk Menekan Populasi Fusarium oxysporum f.sp. cubense Penyebab Busuk Batang Pisang NI KADEK LIA SWANDEWI; I MADE SUDANA; I KETUT SUADA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.8, No.4, Oktober 2019
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Application of Biological Agent and Humus to Suppress Population Fusariumoxysporum f.sp. cubense the Cause of Banana Stem Rot. The study aimed to determine the interaction between P. fluorescens, Trichoderma spp. fungus, and humus in suppressing the population of Fusariumoxysporum f.sp. cubense in the soil. The design used was a randomized blockdesign (RBD) of 2 factors namely antagonistic microbes and humus. There were 18treatments with 3 replications so that 54 experimental units were obtained. Thevariables observed were the population of Foc, population of P. fluorescens,population of Trichoderma spp., and soil pH. The results showed that thecombination of biological agent and humus could reduce the population of Foc. Thehigher concentration of humus combined with biological agent showed thedecreasing population of Foc which causes rot of banana stems.
Isolasi dan Seleksi Bakteri Antagonis untuk Pengendalian Penyakit Busuk Batang Panili (Vanilla planifolia Andrews) Secara In Vitro I MADE ARIMBAWA; GUSTI NGURAH ALIT SUSANTA WIRYA; I MADE SUDANA; I MADE WINANTARA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.8, No.2, April 2019
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Isolation and Selection of Antagonistic Bacteria to Control Stem Rot Disease on Vanilla Plant (Vanilla planifolia Andrews) by In Vitro Test. Isolation, selection, and identification of effective microbes are important steps to obtain biological agents. The perpose of this research was to get potential bacteria as controlling agents for stem rot of vanilla. The research was conducted at Plant Disease Laboratory, Faculty of Agriculture, Udayana University, Denpasar, from December 2017 to February 2018. The research comprised of isolation and identification the pathogen of stem rot of vanilla, pathogenicity test, isolation and identification of antagonistic bacteria, in vitro test of antagonistic bacteria ability to controled the pathogen of stem rot of vanilla, and hypersensitive response of antagonistic bacteria on tobacco plant. The result showed that the pathogen of stem rot on vanilla plants was identified as Fusarium oxysporum. There of 21 isolates of bacteria that has been isolated from field, there were 7 isolates could suppress of F. oxysporum growth are isolates P001, P002, P003, P004, P005, BM02, and BM03. In vitro test of antagonistic bacterial with F. oxysporum showed that isolate P002 could suppress pathogen growth with the highest percentage (98,36%), P005 (98,34%), P001 (92,78%), P003 (82,02%), P004 (80,34%), BM02 (71,80%), and BM03 (66,70%). Hypersensitive respons test showed that isolates P001, P002, P003, P004, P005, BM02, and BM03 weren’t pathogenic for plants.
Co-Authors Gusti Ngurah Alit Susanta Wirya I Ketut Suada I Made Arimbawa I MADE WINANTARA NI KADEK LIA SWANDEWI