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All Journal Jurnal Anestesiologi Indonesia Jurnal Kedokteran Syiah Kuala Oto Rhino Laryngologica Indonesiana Biomolecular and Health Science Journal JUXTA: Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga International Journal of Nasopharyngeal Carcinoma Indian Journal of Forensic Medicine & Toxicology Folia Medica Indonesiana
Yusuf, Muhtarum
Department Of Otorhinolaryngology-Head And Neck Surgery, Faculty Of Medicine, Universitas Airlangga - Dr. Soetomo General Hospital, Surabaya
Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Public Health

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Ekspresi protein P53 dan HSP70 pada sel punca karsinoma nasofaring yang resisten terhadap radioterapi Muhtarum Yusuf; Ahmad Chusnu Romdhoni; Widodo Ario Kentjono; Fedik Abdul Rantam
Oto Rhino Laryngologica Indonesiana Vol 44, No 2 (2014): Volume 44, No. 2 July - December 2014
Publisher : PERHATI-KL

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1061.659 KB) | DOI: 10.32637/orli.v44i2.93

Abstract

Latar belakang: Pada penderita Karsinoma Nasofaring (KNF) masih sering ditemukan kekambuhan meskipun sudah mendapat terapi yang lengkap. Penelitian terbaru membuktikan bahwa kekambuhan disebabkan oleh sel punca KNF yang resisten terhadap radioterapi. Mekanisme resistensi sel punca kanker terhadap radioterapi diduga karena hambatan terhadap apoptosis dan atau memicu proliferasi. Hambatan terhadap apoptosis disebabkan oleh penurunan protein p53 (wild type), selain over-ekspresiHsp70. Tujuan: Menjelaskan mekanisme resistensi sel punca KNF terhadap radioterapi berdasarkan profil ekspresi protein p53(wild type)dan Hsp70. Metode: Penelitian true experimental dengan menggunakan rancangan randomisasi kelompok kontrol sebelum dan sesudah tes. Kultur sel punca KNF dibagi menjadi dua kelompok, masing-masing 16 sampel. Pada kelompok perlakuan diberikan paparan radioterapi dengan dosis 1,5 Gy menggunakan pesawat Linac, lalu diinkubasi selama 24 jam. Sebelum dan sesudah perlakuan pada kedua kelompok diperiksa ekspresi p53 (wild type) dan Hsp70. Pemeriksaan menggunakan metode flowcytometry. Hasil: Ekspresi p53 (wild type) antara kelompok perlakuan dan kontrol terdapatperbedaan yang tidak bermakna dengan p=0,576 (p≥0,05). Ekspresi Hsp70, antara kelompok perlakuan dan kontrol terdapat perbedaan yang tidak bermakna dengan p=0,172 (p≥0,05). Kesimpulan: Tidak terdapatperubahan ekspresi p53 (wild type) dan Hsp70 pada sel punca KNF yang resisten terhadap radioterapi.Kata kunci : Sel punca KNF, p53 (wild type), Hsp70, karsinoma nasofaring ABSTRACTBackground: Recurrences are frequently occurred in nasopharyngeal  carcinoma (NPC) patients, eventhough they had received complete therapy. Recent studies have proved that those recurrences were caused by NPC cancer stem cells that resistant to radiotherapy. Mechanisms of resistance of cancer stem cells to radiotherapy is assumed due to the block of apoptosis and or proliferation inducing. The block of apoptosis was caused by the decrease of p53 (wild type) expression, in addition to Hsp70 over expression. Objective: To find out the mechanism of NPC stem cells that resistant to radiotherapy based on profiles of protein p53 (wild type) and Hsp70 expression. Methods: Using true experimental study by randomizedpre and post test control group design. The cultured NPC stem cells were divided into two groups, with 16 samples each. The treatment group had 1,5Gy dose of radiotherapy exposure with Linac device, then incubated for 24 hours. Both groups were examined for p53 (wild type) and Hsp70 expressions before and after treatment. The examinations were conducted by flowcytometry method. Result: The P53 (wild type) expression between the treatment and control group showed insignificant difference with p=0.576(p≥0.05). The Hsp70 expression between treatment and control group showed insignificant difference with p=0.172 (p≥0.05). Conclusion: There were no changes of p53 (wild type) and Hsp70 expressions on NPC stem cells that resistant to radiotherapyKeywords: NPC stem cells, p53 (wild type), Hsp70, nasopharyngeal carcinoma
Clinicopathological profile of nasopharyngeal carcinoma in 2016-2019 at Dr. Soetomo General Hospital Karindra Amadea Susetiyo; Etty Hary Kusumastuti; Muhtarum Yusuf; Rosa Falerina
Oto Rhino Laryngologica Indonesiana Vol 52, No 1 (2022): VOLUME 52, NO. 1 JANUARY - JUNE 2022
Publisher : PERHATI-KL

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32637/orli.v52i1.474

Abstract

ABSTRACTBackground:.The number of nasopharyngeal carcinoma (NPC) cases is increasing and causing death, placing this cancer in fifth place as the cause of death in Indonesia. The initial clinical symptoms that are less specific often cause patients coming with advanced stage conditions. Therefore, the study of clinical and pathological profiles of NPC patients are required. Objective: To find out the clinicopathological profile of  NPC patients in Dr. Soetomo General Hospital in 2016-2019. Methods: This study was descriptive research with a retrospective study using medical records of NPC patients in Dr. Soetomo General Hospital from 2016 to 2019. Results:  192 NPC patients that meet the criteria were divided according to their age, gender, histopathological classification, stage, and post-therapy response. Out of 192 patients, 22 patients had post-therapy response based on histopathological data. Conclusion: NPC patients in Dr. Soetomo General Hospital in 2016-2019 were found in the range of 14-77 years old, most patients were in the age group 41-≤50 years old (66 patients, 34%). There were more male than female patients (73%). The most common clinical symptoms experienced by patients were a lump on the neck (82%), local ear complaints (79%), and local nasal complaints (75%). The most common histopathological subtype was non-keratinizing squamous cell carcinoma undifferentiated in 181 patients (94%). The highest stage of patients was IV A in 122 patients (64%). In 18 (82%) from 22 patients, based on histopathology examination had good criteria on therapy response. ABSTRAKLatar belakang: Angka kasus karsinoma nasofaring yang (KNF) kian meningkat dan dapat menyebabkan kematian, menempatkan kanker ini pada urutan ke lima di Indonesia. Gejala klinis awalnya yang kurang spesifik seringkali menyebabkan penderita datang dengan kondisi stadium lanjut. Oleh karena itu, profil penderita KNF secara klinis dan gambaran histopatologinya perlu diteliti lebih lanjut. Tujuan: Mengetahui profil klinikopatologi penderita KNF di RSUD Dr. Soetomo tahun 2016-2019. Metode: Penelitian secara deskriptif dengan studi retrospektif menggunakan rekam medis penderita KNF di RSUD Dr. Soetomo tahun 2016-2019. Hasil:  Sebanyak 192 penderita KNF yang memenuhi kriteria dibagi berdasarkan kategori usia, jenis kelamin, klasifikasi histopatologi, stadium, dan respon pasca terapi. Dari 192 penderita ditemukan sebanyak 22 penderita memiliki data respon pasca terapi berdasarkan histopatologi. Kesimpulan: Penderita KNF di RSUD Dr.Soetomo tahun 2016-2019 ditemukan pada rentang usia 14-77 tahun dengan penderita terbanyak pada rentang usia 41- 50 yaitu 66 (34%) serta lebih banyak terjadi jenis kelamin laki-laki yaitu 140 (73%). Gejala klinis yang paling banyak dialami penderita adalah benjolan pada leher 158 (82%), keluhan lokal telinga 151 (79%), dan lokal hidung 144 (75%). Subtipe histopatologi terbanyak adalah nonkeratinizing squamous cell carcinoma undifferentiated yaitu 181 (94%) penderita. Stadium terbanyak penderita adalah IV A yaitu 122 (64%) penderita. Sebanyak 18 (82%) dari 22 penderita berkriteria baik pada respon terapi berdasarkan histopatologi.
COMPARISONS OF CYTOLOGICAL RESULTS FROM BRUSHING METHOD UNDER NASOPHARYNGOSCOPY GUIDANCE AND HISTOPATHOLOGICAL RESULTS FROM BLIND BIOPSY OF NASOPHARYNX IN NASOPHARYNGEAL CARCINOMA PATIENTS Selvianti; Irwan Kristyono; Muhtarum Yusuf
INTERNATIONAL JOURNAL OF NASOPHARYNGEAL CARCINOMA Vol. 1 No. 02 (2019): International Journal of Nasopharyngeal Carcinoma
Publisher : TALENTA PUBLISHER

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (652.314 KB) | DOI: 10.32734/ijnpc.v1i2.1150

Abstract

Introduction: Nasopharyngeal carcinoma (NPC) is one of the common head and neck malignancies found in Indonesia. Diagnosis was based on histopathological result of tumor tissues taken from the nasopharynx. Histopathological from nasopharyngeal blind biopsy remains a golden standard of NPC diagnosis. Blind biopsy causes many problems for patients including pain sensation, risk of bleeding and traumatic effect. Another technique for obtaining specimen for evaluation malignant cells of nasopharynx was by cytological investigation. Cytology evaluation could detect malignant cells from smear specimen. Cytological specimen could be obtained from nasopharynx by brushing method. Cells are collected by brushing then processed with liquid-based technique; specimens were smeared and stained for cytology evaluation. Since nasopharynx location is invisible, then nasopharyngoscopy guidance was used to obtain a brushing specimen. Objective: To analyze an agreement between cytological results from nasopharyngeal brushing under nasopharyngoscopy and histopathological results from blind biopsy as a golden standard for the detection of malignant cells in NPC patients. Method: Diagnostic test with the cross-sectional comparative study. Population of this study was NPC patients who came to outpatient Department of Otorhinolaryngology Head and Neck Surgery Dr. Soetomo Hospital Surabaya. Samples were collected with consecutive sampling method during February until May 2011. During that period we obtained 36 patients as study samples. First we performed brushing method under nasopharyngoscopy guidance and then blind biopsy of nasopharynx on each patient. Cytology preparation processed with liquid-based technique liquiPREP and Papanicolaou stain. Histopathologic preparation used hematoxylin and eosin (H&E) stain. Sample data was tested by McNemar test and Kappa association test. Result: 36 samples were obtained consisted of 27 male (75%) and 9 female (25%). The result for cytological evaluation in detecting malignant cells of NPC patients was sensitivity 87.10%, specificity 80%, positive predictive value 96.43%, negative predictive value 50% and accuracy 86.11%. Statistically, analysis result with McNemar test was no significant difference and from Kappa association test there was a significant association between brushing method under nasopharyngoscopy guidance and blind biopsy. Conclusion: There was an agreement between brushing method under nasopharyngoscopy guidance and blind biopsy in detecting malignant cells of NPC.
The correlation between IL-10 expression and histopathological type in nasopharynx carcinoma patients Hamita Hamita; Muhtarum Yusuf; Manshur Shidiq Wiyadi
Oto Rhino Laryngologica Indonesiana Vol. 50 No. 1 (2020): Volume 50, No. 1 January - June 2020
Publisher : PERHATI-KL

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32637/orli.v50i1.353

Abstract

Background: Nasopharyngeal carcinoma (NPC) is a malignancy originated from nasopharyngeal epithelial cells. NPC therapy response could be predicted from histopathological type, but some patients with the same histopathological type, showed a different therapy response. Interleukin (IL)-10 expression is expected to be able to predict a better response of therapy in NPC patients. Purpose: To find out the correlation between IL-10 expression and histopathological type in NPC patients. Method: An analytic observational study with cross sectional approach towards 33 samples taken from the Oncology Polyclinic of Outpatient Unit of Otorhinolaryngology Head and Neck Surgery Department, Dr. Soetomo General Hospital. Formalin-fixed paraffin-embedded biopsy specimens were obtained. The IL-10 expression was studied with immunohistochemistry using rabbit polyclonal antibody Anti IL-10. Assessment of the staining used Allred scale. The Fisher exact test was utilized to determine the correlation of IL-10 expression and histopathological type of NPC, with p value = 0.05. Result: The result of IL-10 expression in NPC patients with histopathological WHO type I NPC obtained 1 sample (8.3%) with strong positive expression and 2 samples (9.5%) with weak positive expression. In patients with histopathological WHO type II NPC obtained 2 samples (16.7%) with strong positive expression and 12 samples (57.1%) with weak positive expression. In patients with histopathological WHO type III NPC obtained 9 samples (75%) with strong expression and 7 samples (33.3%) with weak positive expression. Conclusion: There was moderate positive correlation between IL-10 expression and histopathological type in NPC patients.Keywords: nasopharyngeal carcinoma, IL-10 expression, histopathological type ABSTRAK Latar belakang: Karsinoma nasofaring (KNF) adalah suatu keganasan yang berasal dari epitel nasofaring. Respon terapi KNF selama ini dapat dinilai berdasarkan tipe histopatologi, namun pada kenyataannya penderita KNF dengan tipe histopatologi sama dapat menunjukkan respon terapi berbeda. Pemeriksaan ekspresi interleukin (IL)-10 diharapkan dapat memberikan prediksi lebih baik mengenai respon terapi pada penderita KNF. Tujuan: Mengetahui hubungan antara ekspresi IL-10 dengan tipe histopatologi pada penderita KNF. Metode: Penelitian observasional analitik dengan pendekatan cross sectional pada 33 sampel yang diperoleh dari Poliklinik Onkologi Unit Rawat Jalan, Departemen THTBedah Kepala Leher, RSUD Dr Soetomo. Ekspresi IL-10 diperiksa dari blok parafin dengan teknik pemulasan imunohistokimia menggunakan rabbit polyclonal antibody Anti IL-10. Ekspresi IL-10 dinilai dengan menggunakan skala Allred. Uji Fisher exact digunakan untuk menentukan korelasi antara ekspresi IL-10 dan tipe histopatologi KNF, dengan p = 0,05. Hasil: Ekspresi IL-10 pada KNF WHO tipe I didapatkan ekspresi positif kuat 1 penderita (8,3%) dan ekspresi positif lemah 2 penderita (9,5%). Hasil ekspresi IL-10 pada KNF WHO tipe II didapatkan ekspresi positif kuat 2 penderita (16,7%) dan ekspresi positif lemah 12 penderita (57,1%). Hasil ekspresi IL-10 pada KNF WHO tipe III 9 penderita (75%) dengan ekspresi positif kuat dan 7 penderita (33,3%) dengan ekspresi positif lemah. Kesimpulan: Didapatkan korelasi positif sedang antara ekspresi IL-10 dan tipe histopatologi pada penderita KNF
Laryngomalacia: diagnosis and management at Otorhinolaryngology Head and Neck Surgery Department Dr.Soetomo Hospital, Surabaya Muhtarum Yusuf; Puji Utami
Oto Rhino Laryngologica Indonesiana Vol. 50 No. 2 (2020): Volume 50, No. 2 July - December 2020
Publisher : PERHATI-KL

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32637/orli.v50i2.405

Abstract

ABSTRACTBackground: Laryngomalacia is an abnormality of the larynx that leads to the inward collapse of the supraglottic structure during inspiration. The condition is primarily characterized by congenital stridor in infants and children. Diagnosis is often made based on the larynx visualization during inspiration. Laryngomalacia is generally recovers spontaneously at the age 2-5 years old, surgery is reserved for severe cases. Purpose: To obtain data of the clinical appearance, diagnosis, and therapy of laryngomalacia. Method: An observational study on medical records of all laryngomalacia patients at the Department of Otorhinolaryngology Head and Neck Surgery, Faculty of Medicine Universitas Airlangga /Dr. Soetomo General Hospital, Surabaya from January 1st, 2017 to December 31st, 2018. Data evaluation included clinical symptoms, type of laryngomalacia, comorbid factors, the age range of clinical improvement, and the management. Result: The main complaint of laryngomalacia was inspiratory stridor in 63 patients (82.89%), followed by dyspnea, snoring, hoarseness, and choking. Endoscopy finding mostly was type 1 in 69 patients (90.79%), the others were type 2 and 3. Comorbid factor mostly was neurological disease in 16 patients (21.03%), followed by congenital heart disease, congenital abnormality, prematurity, and laryngopharyngeal reflux. The age group of clinical improvement majority were <12 months, followed by >12-24 months, and no improvement in 56 patients (73.69%). Conservative therapy was conducted in 73 patients (96.05%) and 3 patients were tracheotomized. Conclusion: The main complaint of laryngomalacia was inspiratory noises, mostly found was laryngomalacia type 1.The highest comorbid factor was neurological disease. The most common management was conservative therapy. ABSTRAKLatar belakang: Laringomalasia adalah kolapsnya struktur supraglotik laring saat inspirasi. Laringomalasia umumnya sembuh spontan pada umur 2-5 tahun, pembedahan hanya dilakukan pada kasus yang berat.Tujuan: Memperoleh gambaran klinis penderita laringomalasia, diagnosis dan terapi. Metode: Penelitian observasional pada semua rekam medik yang lengkap penderita laringomalasia  di Departemen THT-KL RSUD Dr. Soetomo, Surabaya, periode 1 Januari 2017 - 31 Desember 2018. Data yang dievaluasi meliputi gejala klinik, tipe, faktor komorbid, usia perbaikan gejala, dan terapi. Hasil: Keluhan utama adalah napas berbunyi pada 63 penderita (82,89%), disusul sesak napas, mengorok saat tidur, suara parau, dan mudah tersedak. Temuan  endoskopik mayoritas tipe 1 pada  69 penderita (90,79%), sisanya tipe 2 dan 3. Faktor komorbid terbanyak penyakit neurologis pada   16 penderita (21,03%), disusul kelainan jantung bawaan, anomali kongenital, prematur, dan refluks laring faring. Usia perbaikan klinis mayoritas <12 bulan, diiikuti >12-24 bulan dan belum membaik 56 penderita (73,69%). Terapi konservatif dilakukan pada 73 penderita (96,05%) dan 3 penderita menjalani trakeotomi. Kesimpulan: Keluhan utama napas berbunyi, mayoritas laringomalasia tipe 1. Faktor komorbid terbanyak penyakit neurologis. Laringomalasia mayoritas diterapi secara konservatif.
Correlation between Cell Proliferation with Cervical Lymphoid Node Status in Nasopharyngeal Carcinoma Patients Nugroho, Puguh Setyo; Yusuf, Muhtarum; Hidayati, Titiek Ahadiyah
Folia Medica Indonesiana Vol. 57, No. 1
Publisher : Folia Medica Indonesiana

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Several studies showed that the index of nasopharyngeal carcinoma (NPC) cell growth could be used to assess the carcinogenesis interaction factor, development and prognosis of NPC. Cell proliferation index could always be assessed with Ki-67 protein expression test. This research was conducted to study the correlation between cell proliferation index with cervical lymphoid node status in NPC in clinical manifestation to asses the progressivity and prognosis on NPC patients. This study used cross sectional design. Biopsy tissue specimen were acquired from 35 NPC patients clinically divided into four criteria of cervical lymphoid node status (N0, N1, N2 and N3). Expression of Ki-67 protein was acquired by immunohistochemistry test using monoclonal rabbit antibody anti-human Ki-67 clone 901-325-091911 (Biocare Medical, LCC. 4040 Pike Line, CA 94520 USA). The measurement of Ki-67 protein was conducted by pathology consultant. Spearman statistic test was performed to asses the correlation between Ki-67 protein expression and cervical lymphoid node status. The statistical significance was defined as p<0.05. Positive expression of Ki-67 protein was found in 33 patients; 4 patients with N0 (11.43%), 5 patients with N1 (14.29%), 9 patients with N2 (25.71%), and 15 patients with N3. Negative expression of Ki-67 protein was found in 2 patients with N0 (5.71%). The Spearman test resulted at p=0.0001 with correlation coefficient of 0.758. The correlation between Ki-67 protein expression with cervical lymphoid node resulted in a significant correlation (p<0.05). In conclusion, cell proliferation index has correlation with cervical lymphoid node status in NPC patients.
Co-Authors Ahmad Chusnu Romdhoni Andriana, RA Meisy DYAH FAUZIAH, DYAH Falerina, Rosa Fatimah, Nurmawati Fedik Abdul Rantam Finna Permata Putri Hamita Hamita Hamita, Hamita Hidayati, Titiek Ahadiyah Karindra Amadea Susetiyo KUSUMASTUTI, ETTY HARY Manshur Shidiq Wiyadi Mansur Shidiq Wiyadi Maulydia, Maulydia Nabilah Puspa Utami Nugroho, Puguh Setyo Puji Utami Sabilarrusydi Sabilarrusydi Sarah Izdihar Selvianti Sofia Tiarini Tha&#039;atam Mardhiyah Titiek Ahadiyah Hidayati Turchan, Agus Widodo Ario Kentjono Wiyadi, Manshur Shidiq