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All Journal JOURNAL OF COASTAL DEVELOPMENT Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Journal of Global Pharma Technology
Herawati Sudoyo
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Education Mathematics Medicine & Pharmacology

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BIOPROSPECTING OF BACTERIAL SYMBIONT OF Tunicate Didemnum molle FROM SAMBANGAN, KARIMUNJAWA ISLANDS Miftahuddin Majid Khoeri; Ocky Karna Radjasa; Agus Sabdono; Herawati Sudoyo
JOURNAL OF COASTAL DEVELOPMENT Vol 14, No 3 (2011): Volume 14, Number 3, Year 2011
Publisher : JOURNAL OF COASTAL DEVELOPMENT

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Abstract

Coral reef is a productive ecosystem with high biodiversity in the sea and being targeted to find a useful bioactive compound. However, the serious problem in development of bioactive compounds from marine invertebrate is the supply problem, because to get a small amounts of active compounds a massive numbers of sea organisms are needed. Tunicate is an animal in coral reef ecosystem that produces many bioactive compounds with pharmacological activities, such as, antibacterial, antitumor, and anticancer compounds. It has been reported that bacterial symbionts of coral reef invertebrates may synthesize the same compounds as the host. The purposes of this research are to isolate and to identify microbes which have antibacterial activity against MDR bacteria based PCR 16S rRNA and to detect the existence of  PKS and NRPS biosynthetic gene fragments from tunicate bacteria of Didemnum molle. Out of 15 bacterial isolates, one isolate showed antibacterial potential against Escherichia coli and Staphylococcus sp. Molecular identification result showed that TS2A5 bacterium has a homology of 99 % with Virgibacillus sp. strain GSP17 16S ribosomal RNA gene. This isolate was also capable of amplifying  NRPS gene fragment.
RASIO SEKS DAN SEBARAN SPASIAL POPULASI GAJAH SUMATERA (Elephas maximus sumatranus) DI TAMAN NASIONAL TESSO NILO, RIAU Nico Herlambang; Haris Gunawan; Herawati Sudoyo
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 2, No 1 (2015): Wisuda Februari 2015
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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The Sumatran  Elephant (Elephas maximus sumatranus) is one of the largest mammal and endemic species on the island of Sumatra.  It’s conservation  status  is critically endangered.  The information  about  sex ratio and spatial distribution is an important component to make a priority  in  conservation strategy. The aims  of  this  study  were  to determine sex ratio and spatial distribution of Sumatran Elephant in Tesso Nilo National Park. The multiplex PCR method was used in this study to amplify fragments SRY1 and AMELY2  on the Y chromosome and fragment  PLP1  on the X chromosome  for  sex identification in Sumatran Elephant. The analysis of spatial distribution were conducted using Arc  GIS 10.1. The result indicated that sex ratio of  Sumatran Elephant population in TNNP  is 1:3  and the  distribution of Sumatran Elephant  is generally spread outside of the region TNNP.
ANALISIS MIKROSATELIT PADA SAMPEL FESES GAJAH SUMATERA (Elephas maximus sumatranus) DI TAMAN NASIONAL TESSO NILO, RIAU Beno Fariza Syahri; Haris Gunawan; Herawati Sudoyo
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 2, No 1 (2015): Wisuda Februari 2015
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

Sumatran elephant (Elephas maximus sumatranus) is  an  endemic  land mammal on Sumatra  Island which  now  is  on  critically endangered. The population of Sumatran elephant in Riau Province spread in 9 pouches of population,  one of them is population in  Tesso  Nilo National Park (TNNP).  Habitat fragmentation and  land  conversion  in protected areas caused  condition of Sumatran elephant’s population had  deteriorated. Therefore, we need a precise conservation effort  to save the population of  Sumatran elephant in TNNP. These  efforts  require  appropriate  information on the number of individuals and population genetic. The aims of this study were to determine the number of individuals, variation and frequency  of allele, and  the  level of heterozygosity of Sumatran elephant population in TNNP based on genetic information.  Molecular analysis using microsatellite marker was performed on 108 non-invasive samples which were collected using CMR method. The result revealed that there were 73 individuals of Sumatran elephant in TNNP.  The average number of allele  variation  from 13 microsatelite loci  which were  analized was 2.85 allele/locus  with average observed heterozygosity (0.507)  is higher than  expected heterozygosity  (0.490). Those  indicates that allele frequencies found to be above of the Hardy-Weinberg equilibrium. This studyproved that there is no genetic pressure of Sumatran elephant population in TNNP.
Single Nucleotide Polymorphisms of Transcription Factor 7-LIKE 2 Gene Expressed Different MRNA Isoforms in the Peripheral Blood Made Ratna Saraswati; I Gusti Ngurah Kade Mahardika; Ketut Suastika; Sukma Oktavianthi; Herawati Sudoyo; Safarina G. Malik
Journal of Global Pharma Technology Volume 10 Issue 07: (2018) July 2018
Publisher : Journal of Global Pharma Technology

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Abstract

Background: Single Nucleotide Polymorphisms (SNPs) of the Transcription Factor 7-Like 2 (TCF7L2) diabetes susceptibility gene were located in the noncoding region. Intronic SNP may play a role in mRNA splicing. This study aimed to elucidate whether SNPs of the TCF7L2 gene expressed different mRNA isoforms in the peripheral blood. Methods: Across sectional study was conducted in Legian Bali. A total of 56 subjects were grouped into carrier of TCF7L2SNPs (28 Balinese age 30-74 years,eitherheterozygote or mutant of the rs12255372 SNP [GT or TT], rs7903146 [CT or TT], and rs10885406 [AG or GG])and wild-type of TCF7L2 (28 matched in age and sex Balinese,rs12255372 [GG], rs7903146 [CC], and rs10885406 [AA])which has been identified in the previous study.In this study, the mRNA isolation was done using LS Trizol reagent (Nitrogen®) and variants of the mRNA isoform in the TCF7L2 gene was identified by reverse transcriptase-polymerase chain reaction (RT-PCR) method, using two pairs of overlapping primer.The first primer PCR products were grouped into Group-A, Group-B, Group-C and Group-D. The second primer PCR products were grouped into Group-a, Group-b, Group-c and Group-d. Oral glucose tolerant test (OGTT) was done to both groups.Blood samples were taken during fasting, at 1 hour and 2 hours after the oralglucose load, for blood glucose, and insulin level measurement. Results: There were seven pairs of the group combination including group A-a, A-c, A-d, C-a, C-c, C-dand D-c, and group C-c was the most commonly found among all subjects in this study (42.3%). Subjects with diabetes risk allele were 2.66 times more likely to express group C-ccompared to subjects without diabetes risk allele of SNPs in the TCF7L2 gene (95%CI 1.24–5.72 p=0.011). Mean response of insulin increment after oral glucose load among group C-c was higher compared with the non-group C-c, 84.63±41.01 vs. 62.02±38.09ng/ml (mean difference 22.61 95%CI 0.41–44.80 p=0.046). However, blood glucose level, fasting insulin, HOMA %B and HOMA IR were similar between the two groups. Conclusion: Variants of TCF7L2 gene expressed different mRNA isoformsin the peripheral blood.Compared to subjects without diabetes risk allele of SNPs in the TCF7L2 gene, subject with diabetes risk allele expressed dominant variant isoform mRNA 2.66 timeshigher.The higherresponse of insulin increment after oral glucosewas observedin the dominant variant which may reflectthe early condition of insulin resistant.Keywords: Diabetes, SNP TCF7L2, isoforms mRNA.
Co-Authors Agus Sabdono Beno Fariza Syahri Haris Gunawan I Gusti Ngurah Kade Mahardika I. K. Suastika MADE RATNA SARASWATI . Miftahuddin Majid Khoeri Nico Herlambang Ocky Karna Radjasa Safarina G. Malik Sukma Oktavianthi