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All Journal Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology
Warsidah Warsidah
Marine Science Department, Faculty of Mathematics and Natural Sciences, Universitas Tanjungpura, Pontianak, Kalimantan Barat
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ANTIBACTERIAL ACTIVITY FROM ETHANOL AND ETHYL ACETATE EXTRACTS OF Padina pavonica HAUCK FROM KABUNG ISLAND AGAINST Escherichia coli Warsidah Warsidah; Ikha Safitri; Mega Sari Juane Sofiana; Shifa Helena
Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Vol 18, No 1 (2022): SAINTEK PERIKANAN
Publisher : Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ijfst.18.1.1-6

Abstract

Macroalgae is marine biological resources that play a crucial role and have an important economic value. They synthesize bioactive compounds with different kind of biological activities, such as antioxidant, antitumor, including antibacterial. In the health sector, infectious diseases from bacteria is one of the problems that still increasing. In addition, E. coli besides having the capacity to be pathogenic agent, it also showed mutlidrug resistence (MDR). Antibiotic resistance is a serious problem worldwide. The increase of this phenomenon leads to the exploration of new antibiotics using natural resources as an effort to overcome the problem. Padina pavonica Hauck belongs to Phaeophyceae spreeding along Kabung Island waters, but its existence has not been widely used. The objective of this study was to evaluate antibacterial activity from ethanol and ethyl acetate extract of P. pavonica Hauck from Kabung Island, West Kalimantan against E. coli. The extraction was  made using ethanol and ethyl acetate solvents, and the antibacterial activity test was realized with concentration series of 5 ppm, 10 ppm, 15 ppm, and 20 ppm. The quantitative assessment of the antibacterial test showed that both ethanol and ethyl acetate extracts of P. pavonica Hauck had potential antibacterial activity against E. coli. The inhibition zone of ethanol extract was 11.6 mm, while ethyl acetate extract was 12.6 mm, respectively. These two solvents were included to the bacteriostatic category.
ANTIOXIDANT AND ANTI-INFLAMMATORY ACTIVITIES FROM ETHANOL EXTRACT OF Eucheuma cottonii FROM LEMUKUTAN ISLAND WATERS WEST KALIMANTAN Mega Sari Juane Sofiana; Ikha Safitri; Warsidah Warsidah; Shifa Helena; Sy Irwan Nurdiansyah
Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Vol 17, No 4 (2021): SAINTEK PERIKANAN
Publisher : Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ijfst.17.4.247-253

Abstract

Free radicals contribute to human health problem resulting in various human diseases, including central nervous system injury, cancer, inflammations, and the decrease of organ function related to oxidation. This condition has encouraged the effort of finding new natural antioxidant and anti-inflammatory sources. Macroalgae act as excellent natural resources due to their bioactive potential with diverse applications in various fields. Eucheuma cottonii belonging to Rhodophyceae grow abundantly along Lemukutan Island waters, however, their existence has not been exploited. This study aims to evaluate antioxidant and anti-inflammatory activities of ethanol extract of E. cottonii from Lemukutan Island waters, West Kalimantan. The observation of antioxidant activity was done using the method of DPPH (1,1-diphenyl-2-picrylhydrazyl) with UV-vis spectrophotometer, while anti-inflammatory acyivity activity was determined using the RBCs membrane stability method. The ethanolic extract of E. cottonii had potential antioxidant activity with IC50 of 127.75 ppm and was classified as moderate category. Extracts showed also anti-inflammatory acyivity activity with the concentration of 219.83 ppm. Red macroalgae E. cottonii can be used as potential natural antioxidant and anti-inflammatory agent.
EFFECT OF COOKING METHODS ON THE REDUCTION OF LEAD (Pb) CONTENT IN KEPAH (Polymesoda erosa) SHELLS Warsidah Warsidah; Mega Sari Juane Sofiana; Agus Yuliono; Lucky Hartanti; Dwi Gusmalawati; Ikha Safitri; Risko Risko; Jannati Jannati
Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Vol 18, No 3 (2022): SAINTEK PERIKANAN
Publisher : Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ijfst.18.3.%p

Abstract

Kepah shells have important economic value and are widely used as food ingredients because they contain high nutritional content. Heavy metals that enter the waters will generally be accumulated in sediments and absorbed by biota. As filter feeder, Kepah accumulate heavy metals from the environment that toxic to humans. This study aimed to determine the effect of cooking methods on the concentration of lead (Pb) in Kepah shell. The measurement of Pb content was conducted according to SNI 2345.5-2011 by Atomic Absorption Spectrophotometer (AAS). Result showed that different cooking methods influenced the Pb content in Kepah shells. Frying and boiling processes reduced the Pb concentration of 46.34% and 31.48%, respectively. According to the result, frying suggested as the best cooking method to provide healthful food for consumption.
Co-Authors Dwi Gusmalawati Helena, Shifa Ikha Safitri Jannati Jannati Lucky Hartanti Mega Sari Juane Sofiana Risko, Risko Sy Irwan Nurdiansyah Yuliono, Agus