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Pro-inflammatory cytokine (IL-6) and total count lymphocyte profiles in COVID-19 patients with different severity levels Shinta Dewi Permata Sari; Wening Tri Mawanti; Dewi Martalena; Erlin Listiyaningsih; Rizkyana Avissa; Rini Latifah; Wawang S Sukarya
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 53, No 3 (2021)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

COVID-19 is caused by the SARS-CoV-2 infection that attacked the human respiratory system. In severe conditions, it causes pneumonia, kidney failure, acute respiratory distress syndrome (ARDS), and even death. The SARS-CoV-2 infection triggers the immune cells to secrete an excess of pro-inflammatory cytokines lead to cytokine storm. It is believed to become one of the mechanisms that cause the ARDS condition. The level of pro-inflammatory cytokines will differ with each case severity. This study aimed to evaluate the profile of pro-inflammatory cytokines in COVID-19 patients with different severity. Therefore, it could be used as therapeutic approach for cytokine storm conditions. It was a cross sectional study using plasma samples of COVID-19 patients from Jakarta Islamic Hospital, Pondok Kopi and Dr. M. Goenawan Partowidigdo Hospital, Cisarua, Indonesia. The COVID-19 patients with severe (n=20) and mild to moderate (n=25) severity were involved in this study. As negative control plasma sample from healthy subjects (n=13) was used. Plasma IL-6 levels were measured using the ELISA technique and plasma lymphocyte levels were measured using a hematology analyzer. The results showed that no significant difference between severity and gender was observed (p=0.256). Meanwhile, there is a significant difference in IL-6 level between negative control, mild-moderate, and severe categories (p=0.015). The average IL-6 level in severe categories was higher than mild-moderate and negative control categories, with values 105.375, 59.75, and 64.577 pg/mL, respectively. This result becomes supporting evidence that there is a cytokine storm condition in severe COVID-19 patients. Furthermore, the lymphocyte level in the severe group is significantly lower than the mild to moderate group. This result may indicate lymphocytopenia in the severe group.

The Relation of TMPRSS2 Gene Polymorphism to COVID-19 Severity of Indonesian Population in Jakarta Erlin Listiyaningsih; Rizkyana Avissa; Shinta Dewi Permata Sari; Wening Tri Mawanti; Dewi Martalena
BIOEDUSCIENCE Vol 6 No 3 (2022): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Background: The COVID-19 disease caused by the SARS-CoV-2 virus has diverse symptoms, ranging from asymptomatic, mild symptoms such as flu-like illness and pneumonia to acute respiratory distress syndrome, which ends in death. Until now, the mechanism of the COVID-19 disease that causes widespread symptoms and the severity and factors that influence it are still unclear. During viral internalization, it needs to be cleaved by the serine protease encoded by the TMPRSS2 gene. It is hypothesized that higher expression of the TMPRSS2 gene causes higher virus internalization into cells, leading to more severe symptoms in patients. Methods: The Single Nucleotide Polymorphism Genotype Test was carried out to prove whether the TMPRSS2 gene affects the severity of COVID-19, as evidenced in other viral respiratory diseases. With a better understanding of gene expression related to this disease, it is hoped that we can better understand the mechanism of COVID-19 and establish better therapies and prevention against it. In this study, 68 COVID-19 patients participated and were categorized into two groups based on their clinical symptoms, namely mild symptoms without symptoms (n=12) and Moderate-Severe symptoms (n=56). PBMC cells were isolated from the patient. Then the DNA was extracted and used as a template in the SNP Genotyping of the TMPRSS2 rs2070788 gene variant. Results: The results showed that 35 samples had A/A homozygous genotypes, 29 A/G heterozygous samples, and 4 G/G homozygous samples. n=29) and heterozygous A/G (n=23), whereas only 4 were homozygous G/G. In addition, the homozygous G/G genotype was only detected in the moderate-severe group. Conclusions: A more significant number of samples from the asymptomatic mild symptom group is needed to statistically prove that homozygous G/G variants or G alleles are generally associated with the severity of COVID-19 patients.

The Relation of TMPRSS2 Gene Polymorphism to COVID-19 Severity of Indonesian Population in Jakarta Erlin Listiyaningsih; Rizkyana Avissa; Shinta Dewi Permata Sari; Wening Tri Mawanti; Dewi Martalena
BIOEDUSCIENCE Vol 6 No 3 (2022): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Background: The COVID-19 disease caused by the SARS-CoV-2 virus has diverse symptoms, ranging from asymptomatic, mild symptoms such as flu-like illness and pneumonia to acute respiratory distress syndrome, which ends in death. Until now, the mechanism of the COVID-19 disease that causes widespread symptoms and the severity and factors that influence it are still unclear. During viral internalization, it needs to be cleaved by the serine protease encoded by the TMPRSS2 gene. It is hypothesized that higher expression of the TMPRSS2 gene causes higher virus internalization into cells, leading to more severe symptoms in patients. Methods: The Single Nucleotide Polymorphism Genotype Test was carried out to prove whether the TMPRSS2 gene affects the severity of COVID-19, as evidenced in other viral respiratory diseases. With a better understanding of gene expression related to this disease, it is hoped that we can better understand the mechanism of COVID-19 and establish better therapies and prevention against it. In this study, 68 COVID-19 patients participated and were categorized into two groups based on their clinical symptoms, namely mild symptoms without symptoms (n=12) and Moderate-Severe symptoms (n=56). PBMC cells were isolated from the patient. Then the DNA was extracted and used as a template in the SNP Genotyping of the TMPRSS2 rs2070788 gene variant. Results: The results showed that 35 samples had A/A homozygous genotypes, 29 A/G heterozygous samples, and 4 G/G homozygous samples. n=29) and heterozygous A/G (n=23), whereas only 4 were homozygous G/G. In addition, the homozygous G/G genotype was only detected in the moderate-severe group. Conclusions: A more significant number of samples from the asymptomatic mild symptom group is needed to statistically prove that homozygous G/G variants or G alleles are generally associated with the severity of COVID-19 patients.

Peningkatan Pengetahuan dan Deteksi Dini Obesitas pada Remaja di SMA Muhammadiyah 3, Jakarta Shinta Dewi Sari; Muhamad Arif Budiman; Roito Elmina Gogo Harahap; Gitrif Qonsolanisota; Rido Dawami; Tasya Alleandra
Jurnal SOLMA Vol. 12 No. 1 (2023)
Publisher : Universitas Muhammadiyah Prof. DR. Hamka (UHAMKA Press)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/solma.v12i1.9952

Background: Obesitas merupakan salah satu penyakit yang memiliki prevalensi cukup tinggi di Jakarta dan dapat dialami oleh berbagai kalangan, mulai dari anak-anak, remaja, hingga dewasa. Sebagian besar remaja di kota Jakarta terbiasa mengkonsumsi makanan cepat saji, memiliki pola hidup sedentary, serta adanya penurunan aktivitas akibat pandemi memicu timbulnya risiko obesitas pada remaja. Kegiatan pengabdian masyarakat ini bertujuan untuk memberikan edukasi mengenai obesitas dan pemeriksaan dini obesitas pada remaja guna mencegah terjadinya obesitas remaja maupun mencegah keberlanjutan obesitas dari remaja hingga dewasa. Method: Kegiatan pengabdian masyarakat dilakukan di SMA Muhammadiyah 3, Jakarta dengan jumlah peserta sebanyak 24 siswa kelas 12 IIS1. Kegiatan ini berupa pemberian penyuluhan mengenai obesitas dan pemeriksaan tinggi badan, berat badan, dan lingkar pinggang guna mengetahui status IMT para siswa dan deteksi dini kondisi obesitas. Hasil: Hasil pengukuran IMT menunjukkan bahwa sekitar 41% siswa memiliki status IMT normal, sekitar 24% memiliki status obesitas, 19% dengan status IMT overweight, dan 16% dengan status IMT underweight. Siswa dengan status IMT overweight dan obesitas harus menjaga pola makan dan meningkatkan aktivitas fisiknya. Hasil rerata penilaian post-test dan pre-test menunjukkan adanya peningkatan pemahaman dari para siswa mengenai materi obesitas setelah diberikan penyuluhan/penjelasan materi. Kesimpulan: Penyuluhan menjadi sarana yang cukup efektif untuk meningkatkan pemahaman para siswa mengenai materi obesitas dan konsumsi makan-makanan yang bergizi baik serta rutin melakukan aktivitas fisik perlu dilakukan oleh para siswa SMA Muhammadiyah 3 guna mencegah terjadinya obesitas yang berkelanjutan hingga dewasa.

Specific Primer Design to Detect Connexin-36 Gene in Rattus Norvegicus Brain Sri Suciati Ningsih; Alfina Septiasari; Endin Nokik Stujanna; Rizkyana Avissa; Irena Ujianti; Shinta Dewi Permatasari; Wawang S. Sukarya
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 7 No 1 (2024): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v7i1.17985

Connexin-36 (Cx36) merupakan salah satu protein kanal antar sel. Protein ini paling banyak ditemukan pada otak. Gen Cx36 mengekspresikan protein penghubung Cx36 yang membentuk sinaps listrik. Penelitian ini bertujuan untuk memperoleh desain primer terbaik untuk menganalisis ekspresi Cx36 pada otak tikus dengan metode Quantitative Real Time PCR (qRT-PCR). Jenis penelitian ini adalah penelitian true experimental menggunakan mRNA dari 4 otak tikus. Analisis in silico menggunakan tools Primer 3 dan OlygoAnalizerTM by IDTdengan menggunakan database gen pada NCBI. Tiga primer dengan kriteria yang paling optimum kemudian dievaluasi dengan metode qRT-PCR. Berdasarkan hasil penelitian ketiganya mampu mengamplifikasi gen Cx36 dengan menggunakan qRT-PCR. Hasil primer terbaik menunjukkan melt curve tunggal pada primer no.5 gen Cx36 pada kisaran Tm 80-81oC. Hasil perbandingan uji in silico primer Cx36 yang sudah pernah dipublikasi sebelumnya menunjukkan bahwa primer no. 5 juga memiliki nilai optimal pada setiap kriteria. Maka hasil desain primer yang memiliki spesifisitas dan efektifitas yang optimum untuk mengamplifikasi gen Cx36 pada otak tikus Rattus norvegicus ialah primer no.5 (F: 5â€™-ATTTCCCGCTTCTACATCATCCAAG-3â€™ dan R: 5â€™-CACAGCAAACATGAACACCAGAAAG-3â€™).

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