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All Journal e-Journal Pustaka Kesehatan Journal of Dentistry Indonesia STOMATOGNATIC- Jurnal Kedokteran Gigi ODONTO Dental Journal INDONESIAN JOURNAL OF PHARMACY Dental Journal (Majalah Kedokteran Gigi) Health Notions MDJ (Makassar Dental Journal) Denta: Jurnal Kedokteran Gigi Cakradonya Dental Journal Odonto dental journal Dental Agromedis
Sumono, Agus
Basic Dentistry Department, Dentistry Faculty, Universitas Jember
Dentistry Health Professions Library & Information Science Medicine & Pharmacology Nursing Public Health

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Journal : Odonto dental journal

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THE EFFECTIVENESS OF ANCHOVY INTAKE ON EPITHELIAL SOCKET THICKNESS POST EXTRACTION Tecky Indriana; Agus Sumono; Kunti Sholihah
Odonto : Dental Journal Vol 9, No 1 (2022): July 2022
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (551.283 KB) | DOI: 10.30659/odj.9.1.40-45

Abstract

Background: Epithelial formation or re-epithelialization is one of the parameters in the wound closure. Nutrition has become a major factors in the successful outcome during this process. Anchovy (Stolephorus sp.) contains several proteins, vitamins, and minerals that can act as supplements to support wound closure. This research proposed to understand the effect of anchovy intake toward epithelial thickness of the rats post tooth extraction.Method: This research was an experimental laboratory with a post-test-only control group design using 24 rats as a sample. Rats were divided into 2 groups, the control and the treatment group with 12 rats each. The mandibular left first molar of the samples was extracted, then given with aquadest (the control) and anchovy powder (the treatment) during 3, 5, and 7 days. All rats were decapitated after 24 hours from the last treatment, followed by tissue processing and staining with Hematoxylin-Eosin (HE) for examination. A microscope that connects with optilab on a magnification of 100x was used to measure the epithelial thickness on the thickest and thinnest part of the epithelial. The data were analysed with One-way ANOVA test and LSD test. Result: The results showed that the epithelial thickness of the treatment group was significantly increased compared to the control group (p<0.05). Conclusion: This study concludes that the anchovy (Stolephorus sp.) intake affects increasing epithelial socket thickness of the rats post tooth extraction.
Cytotoxicity analysis of alginate impression materials based red seaweed extract on cultured gingival fibroblast cells Praharani, Depi; Barid, Izzata; Indahyani, Didin Erma; Probosari, Niken; Lestari, Sri; Sulistiyani, Sulistiyani; Sumono, Agus
Odonto : Dental Journal Vol 11, No 2 (2024): December 2024
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.11.2.290-297

Abstract

Background: Alginate is an impression material that is widely used in dentistry. Alginate can actually also be obtained from natural materials such as red seaweed. The impression procedure causes the impression material to come into contact with oral tissues including the gingiva. Ideally, the materials used must also meet requirements such as low toxicity or non-toxicity so that tissue damage does not occur. This study aims to analyze the cytotoxicity of alginate impression materials from red seaweed extract in gingival fibroblast cell. Method: This experimental laboratory design using post-test only control group design. The research groups consisted of: sodium alginate extract group, red seaweed extract-based alginate impression material, positive control and negative control. Cytotoxicity was tested on gingival fibroblast cell cultures and the effect was analyzed using the MTT assay. Exposure to gingival fibroblast cell cultures was differentiated in three time durations: 5 minutes, 10 minutes and 15 minutes. Each time duration was repeated three times. MTT-formazan production is a method used to measure cell viability (living cells). The data obtained were statistically analyzed using two-way ANOVA test and Tukey HSD post hoc test. Result: There was no significant difference in the average cell viability between the red seaweed extract-based alginate impression material group and the negative control group at an exposure duration of 5 minutes, which was more than 90%. Conclusion: The red seaweed extract-based alginate impression material has no toxic effect on gingival fibroblast cells at 5 minutes exposure.
Co-Authors Agustin Wulan Agustin Wulan SD Ahmad Masruri Amiyatun Naini Banun Kusumawardani Budi Yuwono Budi Yuwono Depi Praharani Didin Erma Indahyani Disaera Distrina Fitrian Sari Durrotul Lamiah Dwi Warna Aju Fatmawati Fadhila Zidni Ilma Fitria Arifka Rahman Halimatus Sa'diyah Hasyim Halimatus Sa'diyah Hasyim I Dewa Ayu Ratna Dewanti Izzata Barid Kharishah Muslihah Kunti Sholihah Leliana Sandra Devi Ade Putri Lusi Hidayati, Lusi Muhammad Nurul Amin Nafisah Nafisah Niken Probosari Nurud Diniyah R. Rahadyan Parnaadji R. Rahardyan Parnaadji Raditya Nugroho Rizqiyatul Amiliyah Roedy Budirahardjo Safira Annisa Yasmin Pambudi Sri Lestari SULISTIYANI SULISTIYANI Tecky Indriana Titis Mustikaningsih Handayani Yuni Mulya Utami