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All Journal e-GIGI e-Journal Pustaka Kesehatan Dentika Dental Journal Jurnal Kesehatan Journal of Dentistry Indonesia STOMATOGNATIC- Jurnal Kedokteran Gigi ODONTO Dental Journal Dental Journal (Majalah Kedokteran Gigi) Jurnal Kedokteran Gigi Universitas Padjadjaran Padjadjaran Journal of Dentistry B-Dent, Jurnal Kedokteran Gigi Universitas Baiturrahmah Jurnal Ilmu Kedokteran dan Kesehatan Padjadjaran Journal of Dental Researchers and Students Cakradonya Dental Journal Odonto dental journal
Depi Praharani
Fakultas Kedokteran Gigi Universitas Jember
Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Immunology & microbiology Library & Information Science Medicine & Pharmacology Nursing Public Health

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PENGARUH JUMLAH KOLONI BAKTERI PLAK SUBGINGIVA TERHADAP JUMLAH TOTAL LEUKOSIT SELAMA KEHAMILAN Praharani, Depi
STOMATOGNATIC- Jurnal Kedokteran Gigi Vol 7, No 1 (2010)
Publisher : Fakultas Kedokteran Gigi Universitas Jember

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Abstract

Gingivitis can occur during pregnancy. It is caused by subgingival plaque bacterial. They can enhance because level of estrogen and progesterone are elevated during pregnancy. At patient gingivitis showed that total count of leucocyte is bigger than patients with normal periodontal tissues. The objective of this study was to investigate the influence of the number of subgingival plaque bacterial colonies to total count of leucocyte in blood periphery. Subgingival plaque samples were collected from buccal surface of maxillary first molar. Hereinafter blood taken away from vein periphery and counted by total count leucocyte. The obtained data were analyzed with linear regression test. Statistical analysis showed that the influence of the number of subgingival plaque bacterial colonies to total count of leucocyte in blood periphery was found to be significant (P<0.05). The conclusion is that the number of subgingival plaque bacterial colonies can increase total count of leucocyte in blood periphery.
PENGARUH PEMAPARAN Entamoeba gingivalis TERHADAP JUMLAH POLIMORFONUKLEAR NEUTROFIL (PMN) PADA TIKUS WISTAR JANTAN DENGAN RADANG GINGIVA Dyaningsih, Dewi Maya; Praharani, Depi
STOMATOGNATIC- Jurnal Kedokteran Gigi Vol 9, No 1 (2012)
Publisher : Fakultas Kedokteran Gigi Universitas Jember

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Abstract

Entamoeba gingivalis is one type of parasite that found in the gingival inflammation. Polymorphonuclearneutrophil (PMN) play a role at the beginning of inflammation. This study aims to determine the effect of exposure E.gingivalis to the number of PMN in Wistar male rats with gingival inflammation. The cervical of mandibular incisor ofall experimental animals was ligated with a wire to get the state of gingival inflammation and then they divided intotwo groups, namely control and treatment group. The control group was exposed to the physiological solution,whereas the treatment group were exposed to E. gingivalis for six days. Exposure performed one day after ligated.Data obtained by counting the number of PMN each group on days 4 and 7. The results show that the number ofPMN on the day of the 4th and 7th there was no significant difference between control and treatment group. Theconclusion of this study is the exposure of E. gingivalis did not affect the number of PMN in Wistar male rats withgingival inflammation.
PENGARUH PASTA GIGI EKSTRAK DAUN TEH (Camelia sinensis L) TERHADAP JUMLAH KOLONI Streptococcus sp. PADA PERMUKAAN GIGI Setyorini, Dyah; Praharani, Depi; Kurniawati, Handian E
STOMATOGNATIC- Jurnal Kedokteran Gigi Vol 8, No 1 (2011)
Publisher : Fakultas Kedokteran Gigi Universitas Jember

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Abstract

Background. The primary step of caries process (cafited tooth) is plaque deposition on the surface area of teeth. The first variety of bacteria colonized in plaque is Streptococcus. This bacteria will produce sticky extracelluler gel which could trap another bacterias. The most effective method to prevent plaque accumulation is using mechanical way, such as brushing teeth. Herbal addition at toothpaste is expected to inhibit plaque deposition. Tea is one of herbal containing flavonoid which has an antibacterial. PURPOSE. This research is purposed for knowing the effect of toothpaste containing tea leaf extract (camelia sinesis L) to colony amount of Streptococcus sp bacteria on the surface area of teeth. METHOD. The type of this research is laboratory experiment using post test control group design in research approach. The subjects is 10 children under criterias, who are given an instruction of the proper way in brushing teeth at the first day. on the eighth, ninth, tenth and eleventh days, subjects are instructed brushing their teeth using placebo toothpaste, toothpaste containing tea leaf extract in 5%, 10% and siwak toothpaste (positive control), then gargling twice. Take the plaque sample on the surface area of teeth after 1 hour. The plaque sample will be diluted and inoculated in agar media of Streptococcus. RESULT. Decreasing amount of colonyof Streptococcus sp. after brushing teeth using toothpaste containing tea leaf extract
STATUS KEBERSIHAN MULUT DAN KESEHATAN PERIODONTAL PASIEN YANG DATANG KE KLINIK PERIODONSIA RSGM UNIVERSITAS JEMBER PERIODE AGUSTUS 2009 – AGUSTUS 2010 Praharani, Depi; Ermawati, Tantin; Pujiastuti, Peni
STOMATOGNATIC- Jurnal Kedokteran Gigi Vol 8, No 3 (2011)
Publisher : Fakultas Kedokteran Gigi Universitas Jember

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Level of oral hygiene have a close relationship with periodontal health status; where good oral hygiene will minimize the occurrence of periodontal disease. Aims of this study were to assess the oral hygiene status and periodontal health patients that come to Department of Periodontics of Jember University Dental Hospital at August 2009 until August 2010. This type of research is an observational study of clinical (cross sectional). A total of 155 sample (87 males and 66 females) were examined by using Oral Hygiene Index-Simplified (OHI-S) and Periodontal Index. Good oral hygiene was seen in 11% of total sample examined, 54,2% had fair oral hygiene and rest 34,8% showed poor oral hygiene. 0,6% of total sample examined had healthy periodontal, 18,1% had simple gingivitis, 41,3% had initial of periodontal disease destructive, 37,45 had periodontal disease destructive and 2,6% had terminal disease. Oral hygiene status and periodontal health examination revealed that majority of sample had fair oral hygiene and periodontal disease destructive.
PENGARUH PENYAKIT PERIODONTAL SELAMA MASA KEHAMILAN TERHADAP JUMLAH TOTAL LEUKOSIT DAN HITUNG JENIS LEUKOSIT Nuarita, Rusliyana; Praharani, Depi; Kusumawardani, Banun
STOMATOGNATIC- Jurnal Kedokteran Gigi Vol 9, No 3 (2012)
Publisher : Fakultas Kedokteran Gigi Universitas Jember

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Abstract

The changes of hormonal during pregnancy can increase the hormones estrogen and progesterone. Increased estrogen and progesterone can cause excessive response in periodontal tissues so easily happen inflammation of the periodontal tissues. The most important aspects of the inflammation is the leukocyte reaction. The purpose of this study was to determine the effect of periodontal disease during pregnancy to the total number of leukocytes and leukocyte counts. Research procedures performed each trimester which includes examining periodontal index (PI) to assess the severity of periodontal disease and total leukocyte counts and leukocyte counts were taken from venous blood. The data obtained were analyzed with linear regression test. Statistical analysis showed that the higher the score PI will increase the total number of leukocytes. The conclusion of this study is periodontal disease during pregnancy may increase the total number of leukocytes and leukocyte counts.
Influence test of Averrhoa Bilimbi leaf extract as denture cleanser on the growth of Streptococcus Mutans Kristiana, Dewi; Praharani, Depi
Padjadjaran Journal of Dentistry Vol 23, No 3 (2011): November
Publisher : Faculty of Dentistry Universitas Padjadjaran, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1566.657 KB) | DOI: 10.24198/pjd.vol23no3.14041

Abstract

It has been believed for a long time, especially by Indonesian people, that Averrhoa Bilimbi leaves have medical efficacy. Streptococcus mutans (S. mutans) are bacteria mostly found in plaque. The plaque on denture may cause inflammation in a mucosal tissue under the denture namely denture stomatitis. This study was aimed at observing the differences of Averrhoa Bilimbi leaf extract efficacy as denture cleanser in a concentration of 4%, 8%, 16% for 15 minutes, 30 minutes, 1 hour, dan 8 hours on the growth of S. mutans. The samples were made from heat-cured resin acrylic plates with the dimension of 10x10x1 mm. Heat-cured resin acrylic plates were immersed in Averrhoa Bilimbi leaf extract with the concentration of 4%, 8%, 16% for 15 minutes, 30 minutes, 1 hour, dan 8 hours which each concentration contained 8 samples, and heat-cured resin acrylic plate was immersed in the aquades as the control. To observe the inhibiting force of Averrhoa Bilimbi leaf extract on the growth of S. mutans, it was tested using the spectrometer. The data were analyzed using one-way ANOVA. The result showed that there were significant differences (p< 05). Then the data were analyzed using LSD test and it showed that there were significant differences on heat-cured resin acrylic plates immersed in Averrhoa Bilimbi leaf extract with the concentration of 4%, 8%, 16% for 15 minutes, 30 minutes, 1 hour, dan 8 hours on the growth of S. mutans. In conclusion, Averrhoa Bilimbi leaf extract in the concentration of 16% used to immerse the acrylic plate for 8 hours effectively inhibited the growth of S. mutans.
ACTIVITY TEST OF IMMUNOMODULATORY COMPONENT FROM NEEM LEAVES TO CANDIDA ALBICANS: UJI AKTIVITAS KOMPONEN IMUNOMODULATOR DARI NEEM TERHADAP CANDIDA ALBICANS I Dewa Ayu Ratna Dewanti; I Dewa Ayu Susilawat; Purwanto; Depi Praharani
Dentika: Dental Journal Vol. 16 No. 2 (2011): Dentika Dental Journal
Publisher : TALENTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (312.333 KB) | DOI: 10.32734/dentika.v16i2.1845

Abstract

Empirically, societies have utilized neem plant to treat worm infection, scabies, malaria, candidal infection, and allergy. Several researches have indicated that consuming neem leaves could improve antibody production. Our previous research had demonstrated the existence of immunomodulatory component in neem leaves weighted 70 and 100 kDA. The aimed of study was to test the activity immunomodulatory components to Candida albicans. This research was an in vitro study with rats as experimental subject. The rats were divided into three treatment groups and one control group, which were labeled as KO (no treatment), KP1 (inoculated by C. albicans only), KP2 (inoculated by C. albicans and were given the 70 kDA immunomodulatory component), KP3 (inoculated by C. albicans and were given the 100 kDA immunomodulatory component). In day 21, preparations from rats’ tongue were done with immunohistochemistry staining and were analyzed for the activity of TNF-α macrophage phagocytosis and also for calculation of C. albicans colony from tongue swab. The result showed that there was no significant difference between the 70 and 100 kDA immunomodulatory components in increasing activity of TNF-α and macrophage phagocytosis and in decreasing the number of C. albicans colony. It was assumed that those components might actívate NF-κB and AP-1 that play role in protein transcription, which stimulated gene expression like TNF-α, which played role in phagocytosis. In conclusión, 70 dan 100 kDA immunomodulatory components increased the activity of TNF-α and macrophage phagocytosis and also decrease the number of C. albicans colony.
Jumlah Trombosit pada Mencit Diabetes setelah Pemberian Ekstrak Rumput Laut Merah (Rhodophyceae) Dahna Maudita Kartikasari; Didin Erma Indahyani; Depi Praharani
Pustaka Kesehatan Vol 7 No 3 (2019): Volume 7 No.3, 2019
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/pk.v7i3.11350

Abstract

Oxidative stress in diabetes mellitus triggers activation and increases in platelet counts that play a role in the formation of platelet plugs in the blood clotting process. If excessive blood clots occur, there is a risk of cardiovascular disease. Red seaweed extract contains carotenoids, phenols and their derivatives, fikobilin pigments, polysaccharide sulfates, and vitamins. The biggest phenol compound is flavonoids which are compounds that can produce platelet antia-gregation effects. The purpose of this study was to examine the effect of giving red seaweed extract to platelet counts in diabetic mice. The study group consisted of the treatment group of red seaweed extract, normal control, positive control and negative controls. The dosage of seaweed extract as much as 10 mg / 20 grBB is given in condition. The calculation of platelet count is carried out directly with the Brecher Cronkite method using a calculated room Improved Neubauer. The One Way ANOVA test results showed a significance value (p <0.05), that is, the group of diabetic mice given red seaweed extract had a lower platelet count of 280000 μl compared to diabetic mice given metformin as much as 379000 μl. The decrease is due to flavonoids, saponins, alkaloids, β carotene, vitamins and minerals able to stabilize the production of megakaryocytes and platelets, prevent the destruction of pancreatic β cells and prevent free radicals. It was concluded that red seaweed extract can reduce platelet counts of diabetic mice near normal values.
Daya Antibakteri Infusa Daun Asam Jawa (Tamarindus indica Linn) terhadap Streptococcus mutans (Antibacterial Activity of Asam Jawa Leaf Infuse (Tamarindus indica Linn) against Streptococcus mutans Anggi Faradiba; Achmad Gunadi; Depi Praharani
Pustaka Kesehatan Vol 4 No 1 (2016)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Abstract

Background: Dental caries is a mouth diseases caused by the interaction of host, microorganisms, substrate and time. One of the microorganisms that had played a role in occurrence dental caries is Streptococcus mutans bacteria. Infection caused by S. mutans can be treated with an natural antibacterial or synthetic antibacterial, but synthetic antibacterial has side effects in long term use so we need to do some research to find out natural materials that can be used as an antibacterial to minimize the side effects. Asam jawa leaf (Tamarindus indica L) is one of medicinal plants that have been known contains antibacterial compound namely flavonoid, saponin, alkaloid, vitamin C, chlorine and tanin. Objective: Determined the antibacterial activity of asam jawa leaf infuse against S. mutans. Methods: Laboratories experimental with post test only control group design research. Samples in this research divided into 2 control groups that is negative control (sterile aquadest), positive control (chlorhexidine) and 3 treated groups that is 25%, 50% and 100% asam jawa leaf infuse with 5 total samples for each groups. The antibacterial test was paper disc diffusion method. Antibacterial activity showed by the inhibition zone around the paper disc that indicated there is no bacterial growth. Result and Conclusion: Asam jawa leaf infuse had antibacterial activity against S. mutans and the effective concentration of the asam jawa leaf infuse which had antibacterial activity was 100%. Keywords : Antibacterial, asam jawa leaf infuse, S. mutans.
Daya Hambat Ekstrak Daun Pepaya (Carica papaya L.) terhadap Adhesi Bakteri Porphyromonas gingivalis pada Neutrofil (Inhibition of Papaya (Carica papaya L.) Leaves Extract on Adhesion of Porphyromonas gingivalis Bacteria to Neutrophils) Ermita Windya Pratiwi; Depi Praharani; Yuliana Mahdiyah Da'at Arina
Pustaka Kesehatan Vol 3 No 2 (2015)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Abstract

Adhesion process of Porphyromonas gingivalis on neutrophils significantly play a role in the onset of periodontal infection. Papaya leaves contain several active substances that might impact to bacterial adhesion. This study was aimed to determine inhibition potency of papaya leaf extract (PLE) to adhesion of Porphyromonas gingivalis to neutrophils and at different concentration. This was an experimental laboratories in vitro study on neutrophils cells from peripheral blood. Samples divided into 5 group: group I/control (without PLE), group II (PLE 25%), group III (PLE 50 %), group IV (PLE 75%), and group V (PLE 100%). Isolated neutrofil was incubated by PLE in 3 hours, then incubated to the P. gingivalis in 2.5 hours. The index of adhesion was determined by calculating average number of P. gingivalis on 100 neutrophils. The result found that inhibition potency of PLE was significantly different to all groups. The conclusion was PLE inhibited P. gingivalis adhesion on neutrophils; and there were differences of inhibition potency of bacterial adhesion of P. gingivalis bacteria among PLE at 25%, 50%, 75% and 100% concentration, which higher concentration had higher inhibition effect.   Keywords: adhesion, neutrophils, papaya leaf, Porphyromonas gingivalis
Co-Authors Achmad Gunadi Achmad Gunadi Anggi Faradiba Arina, Yuliana Mahdiyah Da'at Ayu Mashartini Prihanti Banun Kusumawardani Daat Arina, Yuliana Mahdiyah Dahna Maudita Kartikasari Desi S. Sari Desi Sandra Sari Dewi Kristiana Dewi Maya Dyaningsih Dewi Maya Dyaningsih, Dewi Maya Didin Erma Indahyani Dwi Prijatmoko Dyah Setyorini Elyda Akhya Afida Misrohmasari Ermita Windya Pratiwi Handian E Kurniawati Handian E Kurniawati, Handian E I Dewa Ayu Ratna Dewanti I Dewa Ayu Susilawat I Dewa Ayu Susilawati Inneke Dwi Lestari Izzata Barid Leni Rokhma Dewi Luh Putu Ratna Sundari Mega Bintang Bella Pertiwi Melok A. Wahyukundari Melok Aris Wahyukundari Melok Aris Wahyukundari Melok Aris Wahyukundari, Melok Aris Miftakhur Rizki Nadie Fatimatuzzahro Nafisah Nafisah Neira N. Sakinah Neira Najatus Sakinah Niken Probosari Niken Probosari Peni Pujiastuti Perdana, Mohammad Daffa Duta Prastifa, Febi Zulaikha Purwanto Purwanto , Putri, Iga Nadya Putu Sadaka Putri Rahayu Rahardyan Parnaadji Raissa Salma Hardiani Rendra Chriestedy Prasetya Rusliyana Nuarita Rusliyana Nuarita, Rusliyana Sakinah, Neira Najatus Sita Amelia Sonya Cut Yumita Putri SULISTIYANI SULISTIYANI Sumono, Agus Tantin Ermawati Tsalats, Favinas Octa Nuri Vanesha Santoso Vindy. W, Imaniar Fitriatasya Yuliana Mahdiyah Daat Arina Yuliana Mahdiyah Da’at Arina