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All Journal e-GIGI e-Journal Pustaka Kesehatan Dentika Dental Journal Journal of Dentistry Indonesia STOMATOGNATIC- Jurnal Kedokteran Gigi ODONTO Dental Journal Dental Journal (Majalah Kedokteran Gigi) Padjadjaran Journal of Dentistry Journal of Dentomaxillofacial Science B-Dent, Jurnal Kedokteran Gigi Universitas Baiturrahmah Jurnal Material Kedokteran Gigi Odonto dental journal Journal of Fisheries & Marine Dental Agromedis

Didin Erma Indahyani

Bagian Biologi Oral, Fakultas Kedokteran Gigi, Universitas Jember

Author-ID : 1157459

Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Immunology & microbiology Library & Information Science Materials Science & Nanotechnology Medicine & Pharmacology Public Health

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SIFAT OSTEOINDUKTIF SILIKA AMORPHOUS SEKAM PADI : OSTEOINDUCTIVITY OF AMORPHOUS SILICA FROM RICE HUSK Didin Erma Indahyani; Zahreni Hamzah; Izzata Barid
Dentika: Dental Journal Vol. 16 No. 2 (2011): Dentika Dental Journal
Publisher : TALENTA

Currently, bone graft is recommended to improve and support the biological repair of bone defects properly. Bone graft was found to cause death in the donor area, long pain, infection, cosmetic deformity, hematoma, the amount of blood loss, injury or damage to the nervous in the donor area. Synthetic bone graft material was developed as a scaffold that serves to template the formation of bone. The ideal scaffold should be biodegradable, osteoconductive and osteoinductive. During there graft materials have not optimally provide the morphological, mechanical properties, biocompatibility and biodegradation, and osteoinductive-osteoinductive. Silica is proven to support and promote primary osteoblast growth, synthesis of matrix proteins, collagen type I, so that it can cause bone formation. Rice husk contains of silica is high enough. Because of abundant material and its potential in bone formation, needs to be done research on silica from rice husk in the manufacture of synthetic bone graft. The purpose of this study was to analyze the amorphous silica from waste rice husks as synthetic bone graft material (scaffold), especially against osteoblasts proliferation. Type of study was an experimental laboratory. This research was conducted by isolating the amorphous silica from rice husk. Primary osteoblast cultures derived from calvaria rats aged 2 days, growing in Alpha Modified Eagle Medium (Î±-MEM) (as negative control), Î±-MEM that in condition with 58S silica (as positive control group) and Î±-MEM condition with silica from rice husk (as treatment group). Osteoblast proliferation was observed with Quick Cell Proliferation Assay Kit for 7 and 14 days. The result showed that the rice husk silica was significantly (p <0.05) osteoinductive, osteoblast cultures demonstrated that its proliferation was higher in the groups that were implanted in the culture medium condition with 58S silica. In conclusion, rice husk amorphous silica is potentially osteoinductive scaffold for synthetic bone graft material.

Jumlah Trombosit pada Mencit Diabetes setelah Pemberian Ekstrak Rumput Laut Merah (Rhodophyceae) Dahna Maudita Kartikasari; Didin Erma Indahyani; Depi Praharani
Pustaka Kesehatan Vol 7 No 3 (2019): Volume 7 No.3, 2019
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/pk.v7i3.11350

Oxidative stress in diabetes mellitus triggers activation and increases in platelet counts that play a role in the formation of platelet plugs in the blood clotting process. If excessive blood clots occur, there is a risk of cardiovascular disease. Red seaweed extract contains carotenoids, phenols and their derivatives, fikobilin pigments, polysaccharide sulfates, and vitamins. The biggest phenol compound is flavonoids which are compounds that can produce platelet antia-gregation effects. The purpose of this study was to examine the effect of giving red seaweed extract to platelet counts in diabetic mice. The study group consisted of the treatment group of red seaweed extract, normal control, positive control and negative controls. The dosage of seaweed extract as much as 10 mg / 20 grBB is given in condition. The calculation of platelet count is carried out directly with the Brecher Cronkite method using a calculated room Improved Neubauer. The One Way ANOVA test results showed a significance value (p <0.05), that is, the group of diabetic mice given red seaweed extract had a lower platelet count of 280000 μl compared to diabetic mice given metformin as much as 379000 μl. The decrease is due to flavonoids, saponins, alkaloids, β carotene, vitamins and minerals able to stabilize the production of megakaryocytes and platelets, prevent the destruction of pancreatic β cells and prevent free radicals. It was concluded that red seaweed extract can reduce platelet counts of diabetic mice near normal values.

Pengaruh Lama Pemberian Distres Kronis terhadap Aktivitas Sel Osteoklas pada Tulang Alveolar Tikus Sprague-Dawley (The Effect of Chronic Distress Duration to Osteoclast Cells Activity in Alveolar Bone of Sprague-Dawley Rats) Maria Devitha; Didin Erma Indahyani; Happy Harmono
Pustaka Kesehatan Vol 3 No 3 (2015)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Excessive and prolonged distress (chronic) can cause damage to periodontal tissues, specifically alveolar bone resorption. Alveolar bone resorption is caused by osteoclast cells activity. The purpose of the research is to determine effect of chronic distress duration to osteoclast cells activity in alveolar bone of male Sprague-Dawley rats attain the age of 3-4 months. The rats were given an electric shock stressor (Electrical Foot Shock) as distress chronic for 7, 14, and 28 days. Stressors were given for 30 minutes at 9 a.m every day during the treatment period. The amount of electrical current is 2-8 mA, 48 V voltages and 0.5 Hz frequency. Osteoclasts activity were observed histologically with 400x magnification using osteoclast Bone Interface (OBI) Index by dividing the length of howship's lacunae with osteoclasts. Result showed signifficant difference at 0.00 (p<0.05) beetwen the variable.Activity of osteoclasts in alveolar bone of Sprague-Dawley rats which were exposed to the stressor is increased when compared to the group that were not exposed to electrical shock stressor, but at day 14 decreased when compared to the other treatment groups. Keyword : chronic distress, osteoclast activity, alveolar bone

Kadar Kolesterol pada Mencit (Mus-Musculus) Diabetes Setelah Konsumsi Ekstrak Rumput Laut Coklat (Phaeophyta) Irene Fransiska; Didin Erma Indahyani; Ari Tri Wanodyo Handayani
Pustaka Kesehatan Vol 8 No 1 (2020): Volume 8 No. 1, 2020
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/pk.v8i1.11349

In the case of Diabetes Mellitus, it will trigger some changes in lipid metabolism and lipoprotein composition, one of them is cholesterol. Cholesterol is a lipid that plays an important role in the structure of cell membranes. Seaweed contains bioactive components of flavonoids which reduce cholesterol levels. Brown seaweed contains bioactive components of alkaloids, flavonoids, triterpenoids, saponins, phenol hydroquinone, tannins, chlorophyll and β-carotene pigments. The bioactive component of brown seaweed also has strong antioxidant activity that can prevent damage caused by oxidative stress on pancreatic β cell function and acts as a trap for various free radicals and can reduce diabetic rat lipid peroxidation. This study to analyze the effect of giving brown seaweed extract on cholesterol levels in diabetic mice.This study was divided into 4 sample groups, namely the normal group, negative controls (diabetic mice), positive controls (diabetic mice + 1.3 mg / 20 gr BB metformin), K1 (diabetic mice + brown seaweed extract). The seaweed dose of 10mg / 20grBB is given on a daily basis for 21 days. Checking blood cholesterol levels in serum using the POD CHOD method and measured using biolysers. Diabetic mice with brown seaweed extract were significantly (p <0.05) lower cholesterol level of 68.94 mg / dL than diabetic mice 81.75 mg / dL. It was concluded that brown seaweed extract had an effect on reducing total cholesterol levels in diabetic mice. Keywords : Cholesterol levels, Diabetic mice, Brown Seaweed

Pengaruh Pemberian Monosodium Glutamat pada Induk terhadap Fase Erupsi Gigi Anak Tikus Wistar (Rattus norvegicus) Generasi Pertama (The Effect of Monosodium Glutamate Administration on Mothers Rat (Rattus norvegicus) to the Tooth Eruption Phase of the Fir Mindiya .; Didin Erma Indahyani; Pujiana Endahl Lestari
Pustaka Kesehatan Vol 5 No 3 (2017)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Background: Monosodium glutamat (MSG) is a kind of food additives which consumes widely in society. It may damage the hypothalamic arcuate nucleus and disturb the endocrin regulation system. This condition may delay the tooth eruption phase. Aim: To analyze the effect of MSG administration on the neonatal rats tooth eruption phase, whose mothers were given MSG during gestational period also during gestational and lactation period, to determine the difference between neonatal male and female tooth eruption phase. Materials and Methods: The first molar of right mandibular of neonatal rats from the control group (K1), the treatment group which mothers were given MSG during pregnant period (K2) and the mothers which were given MSG during pregnant and lactation period (K3) were collected as samples. The samples were examined by periapical rontgent to observe the tooth eruption phase. The result was measured by Corel Draw X3. Results and Conclusion: The tooth eruption phase in the treatment group (K2 and K3) are slower than the control group (K1), and there is no difference between neonatal male and female tooth eruption phase. Kata Kunci: hypothalamic arcuate nucleus, monosodium glutamate, tooth eruption

Level Glukosa Darah pada Mencit Diabetes Setelah Pemberian Ekstrak Rumput Laut Coklat (Phaeophyta) Mega Bintang Bella Pertiwi; Didin Erma Indahyani; Depi Praharani
Pustaka Kesehatan Vol 9 No 2 (2021): Volume 9 No.2, 2021
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/pk.v9i2.12165

Diabetes mellitus (DM) is a disorder of hyperglycemia and glucose intolerance caused by insulin deficiency or insulin disorder. Insulin hormone functions in the formation of glucose as an energy source. Consequently, glucose accumulates in the blood in DM patients and affects increased free radicals within the cell and oxidative stress. In DM patients with an imbalance of oxidants and antioxidants in his plasm, therefore, patients with DM require a large amount of antioxidants intake. One alternative treatment is seaweed. Brown seaweed contains bioactive components such as polyphenols and carotenoids, which exhibit antioxidants and reduce blood glucose levels. The study aimed to analyze the effect of brown seaweed extract on the blood glucose levels in diabetic mice. The study used a research design of pre and post-test control group design. The study was divided into 4 sample groups, namely the normal group, negative controls (diabetic mice), positive control (diabetic mice + 1.3 mg / 20 gr BB metformin), P1 (diabetic mice + brown seaweed extract). The seaweed dose of 10mg / 20grBB is given daily for 21 days. Blood glucose level checks using Glucometer and strip test (Autocheck™). Brown seaweed extract has an effect on reducing blood glucose levels in diabetic mice.

Analisis Maturasi Gigi pada Tikus yang Mengkonsumsi Minyak Didin Erma Indahyani
Pustaka Kesehatan Vol 2 No 2 (2014)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Dental maturation is a process of anorganic mineral apotition and resorption ofprotein and water content in the matrix of teeth during the stage of growth and developoment.Disturbances in the stage will caused abnormalities of tooth e.i. hypoplasia andhypomineralisation that are known tooth hypomaturation. The disorder may be systemic orlocal, because of trauma, infection, hormonal, systemic diseases, nutritional deficiencies, etc.fish oil of lemuru containing Eicosanoid acid (EPA) and docohexanoic acid (DHA) are highenough. The EPA and DHA are antiinflammatory and play a role in the regulation ofsignaling molecules and imunomudulator. The purpose of this study was to analyze thematuration of teeth in rats consuming fish oil of lemuru after periodontal infection induced byLPS during amelogenesis. 30 male Wistar rats aged 5 days, divided into three groups. Thefirst group was rats induced by lypopolisacharide (LPS) in the molar region (it aims to makedental maturation disorder). The second group was rats inducted LPS and given fish oil, andthe third group was not LPS induction and not given fish oil (normal). After the rats is agesof 21 days, were done decaputation. Upper right molar teeth have been taken to doobservation in macroscopic (clinical) and microscopic (SEM). Microscopic andmacroscopic observations done scoring, and statistically analyzed using one-way ANOVAand LSD. The results of this study showed that significantly (p <0.005) rats fed fish oil oflemuru, maturation teeth better than LPS-induced rats. It was concluded that fish oil affectsthe maturation lemuru rat teeth, and prevent tooth maturation disorder is more severe.Key words : fish oil, tooth maturation, amelogenesis, SEM

Minyak ikan Lemuru (Sardinella longicep) menurunkan apoptosis osteoblas pada tulang alveolaris tikus wistar (Fish oil of Lemuru (Sardinella longicep) reduced the osteoblast apoptosis in wistar rat alveolar bone) Didin Erma Indahyani
Dental Journal (Majalah Kedokteran Gigi) Vol. 46 No. 4 (2013): December 2013
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Periodontal disease is caused by periodontopatogen bacteria resulting the alveolar bone damage. The decrease of osteoblasts and the increased of osteoclasts can cause bone destruction. The decrease of osteoblasts, due to a disturbance of differentiation, proliferation and apoptosis. Inflammatory mediators are prostaglandin E2 (PGE2), interleukin-1 (IL-1), IL-6 also tumor necrosis alpha (TNF-α) stimulates osteoblast apoptosis through gene expression, signaling molecules and receptor-forming osteoblasts. Fish oil of Lemuru, which is widely encountered in Indonesian coast, containing n-3 poly unsaturated fatty acids (n-3 PUFAs) are quite high. Consumption of fish oil shown to reduce the expression of PGE2, IL-1, IL-6 and TNF-α. Purpose: The purpose of this study was to examine the effect of Lemuru (Sardinella longicep) fish oil on osteoblast apoptosis of rat alveolar bone induced periodontal infection. Methods: Thirty Wistar rats, male, age 5 days, divided into 3 groups: group I rats induced with normal saline, group II rats induced by LPS, and group III rats induced with lemuru fish oil and LPS. Each group was divided into 2 sub-groups that would be sacrified at 13 days and 21 days of age. Fish oil was given at a dose 1ml/300-350 grams. Lipopolysaccharide (LPS) induced with the purpose to cause periodontal infection in the maxillary buccal fold molar region with dose 5μl LPS/PBS 0.03 ml. After decapitation and decalcification, the maxilla was cut in 5μm thickness. Apoptosis was analyzed on DNA and detected by TUNEL reaction (transferase-mediated digoxigenin-deoxy-UTP nick end labeling). Results: The results showed that apoptosis of osteoblast cells was significantly smaller in rats induced by Lemuru fish oil. Conclusion: The study showed that Lemuru fish oil reduced the osteoblast apoptosis of rats alveolar bone induced periodontal infection by LPS.Latar belakang: Penyakit periodontal akibat bakteri peridontopatogen, menyebabkan terjadinya kerusakan tulang alveolar. Penurunan jumlah osteoblas dan peningkatan jumlah osteoklas mengakibatkan kerusakan tulang. Penurunan jumlah osteoblas disebabkan terjadinya gangguan diferensiasi maupun proliferasi juga apoptosis. Apoptosis osteoblas dimodulasi oleh mediator-mediator inflamatori yaitu prostaglandin E2 (PGE2), interleukin-1 (IL-1), IL-6 juga tumor nekrosis alfa (TNF-α), melalui pengaruhnya pada ekspresi gen, molekul-molekul signaling maupun reseptor pembentukan osteoblas. Minyak ikan Lemuru yang banyak di pesisir Indonesia, banyak mengandung n-3 poly unsaturated fatty acid (n-3 PUFA). Konsumsi minyak ikan terbukti menurunkan ekspresi PGE2, IL-1, IL-6 maupun TNF alfa. Tujuan: Tujuan penelitian ini adalah untuk meneliti pengaruh minyak ikan Lemuru (Sardinella longicep) pada apoptosis osteoblas pada tulang alveolar tikus yang diinduksi infeksi periodontal. Metode: Tiga puluh ekor tikus Wistar, jantan, umur 5 hari, dibagi menjadi 3 kelompok yaitu: kelompok I tikus diinduksi dengan salin normal, kelompok II tikus diinduksi dengan lipopolisakarida (LPS), dan kelompok III tikus dinduksi dengan minyak ikan Lemuru dan LPS. Masing-masing kelompok dibagi menjadi "> 2 sub kelompok yaitu kelompok yang akan didekapitasi pada umur 13 hari dan umur 21 hari. Minyak ikan Lemuru diberikan dengan dosis 1ml/300-350 gram. Lipopolisakarida (LPS) diinduksikan dengan tujuan untuk menyebabkan infeksi periodontal pada buccal fold regio molar rahang atas, dengan dosis 5μl LPS/0,03PBS (konsentrasi 0,02 mg). Setelah didekapitasi dan dekalsifikasi, rahang atas dipotong dengan ketebalan 5μm. Apoptosis dianalisis pada DNA dan dideteksi dengan TUNEL reaction (Transferase-mediated digoxigenin-deoxy-UTP nick end labeling). Hasil: Hasil penelitian menunjukkan bahwa apoptosis sel osteoblas secara bermakna lebih kecil pada tikus yang diinduksi dengan minyak ikan. Simpulan: Penelitian ini menunjukkan bahwa minyak ikan Lemuru (Sardinella longicep) mampu menurunkan apoptosis sel osteoblas pada tikus Wistar yang diinduksi infeksi periodontal dengan LPS.

Lipopolysaccharide (LPS) introduction during growth and development period of rat’s tooth toward the occurrence of enamel hypoplasia Didin Erma Indahyani; Al-Supartinah Santoso; Totok Utoro; Marsetyawan HNE Soesatyo
Dental Journal (Majalah Kedokteran Gigi) Vol. 40 No. 2 (2007): June 2007
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

The aim of this study is to know the effect of lipopoly saccharide (LPS) induction during growth and development period specifically the occurrence of hypoplasia on tooth enamel. 5 day old male wistar rats divided into two groups. Group 1 (control) under went no treatment. Group 2 (treatment) under went LPS induction every 24 hour for 8 days on buccal fold right maxillary first molar. After 21 days old the rats were sacrificed and the tooth was resected. Hypoplasia Hypo calcification Index (HHI) was used to determine the degree of hypoplasia by clinical examination. Radiograph of maxilla was also taken to analyze the apacities of enamel by using COREL DRAW version 11. The result showed that group under went LPS induction hypoplasia occurred on its molar tooth and more radiolucent than control groups. The conclusion is LPS induction during growth and development period of rats tooth causing enamel hypoplasia.

The potency of Andrographis paniculata Nees extract to increase the viability of monocytes following exposure to Porphyromonas gingivalis Yani Corvianindya Rahayu; Didin Erma Indahyani; Sheila Dian Pradipta; Anis Irmawati
Dental Journal (Majalah Kedokteran Gigi) Vol. 52 No. 4 (2019): December 2019
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v52.i4.p219-223

Background: Periodontitis is a chronic infectious disease affecting the global population. In Indonesia, the prevalence of periodontal disease has reached 57.6% across all age groups. The bacterium considered as the orginator factor of periodontitis is Porphyromonas gingivalis (P. gingivalis). Herbal ingredients are currently being promoted as a form of treatment because of the minimal side effects they induce. Andrographis paniculata Nees (ApN) extract produces pharmacological effects, including ones immunomodulatory in character, rendering possible its application as a preparation for treating periodontitis. Purpose: The purpose of the study was to prove the potency of Andrographis paniculata Nees extract in increasing the viability of monocytes following exposure to P. gingivalis. Methods: The sample was divided into four groups, namely; Control negative (C-): monocytes in the medium, not exposed to P. gingivalis; Control positive (C+): monocytes in the medium, exposed to P. gingivalis; Treatment I (AP25): monocytes with 25% ApN extract, exposed to P. gingivalis; Treatment II (AP50): monocytes with 50% ApN extract, exposed with P. gingivalis. The monocytes were exposed to 100 uL P. gingivalis for 4.5 hours and stained with trypan blue. Observations were conducted using an inverted microscope at 200x magnification. The percentage of viable monocytes was calculated based on the ratio of the number of the cells which absorbed trypan blue staining to that which did not. Data was tested using a one-way ANOVA followed by an LSD test. Results: There were significant differences between the treatment groups in the number of viable monocytes (p=0.001) they contained. Monocyte viability was higher in the 25% ApN extract group than that exposed to 50% P. gingivalis. Conclusion: Andrographis paniculata Nees extract demonstrates the potency to increase monocyte viability following exposure to P. gingivalis.

Co-Authors Abul Haris Suryo Negoro Achmad Gunadi Al-Supartinah Santoso Anggraeni, Priska Amelia Anis Irmawati Ari Tri Wanodyo Handayani Catur Putri Kinasih Dahna Maudita Kartikasari Depi Praharani Fitria Arifka Rahman Ganefo, Akhmad Happy Harmono Hendri Jaya Permana Inneke Dwi Lestari Irene Fransiska Izzata Barid Kusnadi Kusnadi Luh Putu Ratna Sundari Maria Devitha Marsetyawan HNE Soesatyo Mega Bintang Bella Pertiwi Mindiya . Nafisah Nafisah Negoro, Abdul Haris Suryo Niken Probosari Niken Probosari Prehatin Trirahayu Ningrum Pujiana Endah Lestari Puspita, Indria Dwi Putu Sadaka Putri Rahayu Rahayu, Prehatin Tri Riris Aria Dewanti Rosi Endah Pratiwi Rudianto Sheila Dian Pradipta Sigiq E. Utami Sri Lestari SULISTIYANI SULISTIYANI Sumono, Agus Totok Utoro Verawati Verawati Wardani SWW, L. Dyah Purwita Wazirotus Sakinah Yani Corvianindya Rahayu Yanuar Nurdiansyah, Yanuar Yenny Yustisia Zahreni Hamzah

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