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Pembentukan Hidroksikarbonat Apatit Oleh Pasta Injeksi Bioactive Glass Berbasis Silika Abu Ampas Tebu Dan Penambahan Karageenan Rumput Laut Didin Erma Indahyani; Izzata Barid; Riris Aria Dewanti
STOMATOGNATIC - Jurnal Kedokteran Gigi Vol 20 No 2 (2023)
Publisher : Fakultas Kedokteran Gigi Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/stoma.v20i2.44009

Root caries is common in the elderly, usually detected late, involving enamel, cementum, and dentin below the cementoenamel junction (CEJ). Remineralisation is used to treat it when cavitation has not occurred. Bioactive glass triggers remineralisation by forming hydroxycarbonate apatite (HCA) at an early stage. Injectable paste preparations are needed to overcome these problems. The use of hyaluronic acid and carrageenan polymers supports the physical and mechanical properties of the paste preparation. The purpose this research to analyse the formation of HCA on the tooth root surface by administering bioactive glass (BAG) injection paste with the addition of seaweed carrageenan. This study was a laboratory experimental study with three research groups, namely treatment group 1 (BAG paste and hyaluronic acid), treatment 2 (BAG paste, hyaluronic acid and carrageenan), and control group (BAG without polymer). All groups were immersed in artificial saliva, incubated at 37oC for six days and then tested by Scanning Electron Microscope (SEM) to see the formation of HCA and FTIR. HCA was quantified by a scoring method according to predetermined score criteria. SEM showed HCA formation in both treatment groups, not the control group. There is HCA formation on the tooth surface by administering BAG injection paste with seaweed carrageenan.

The Application of Al-Qahtani Method for Dental Age Estimation of Down Syndrome Children Tridiyanti, Frita Rahima; Apriyono, Dwi Kartika; Barid, Izzata
Jurnal Kesehatan Vol 15 No 1 (2024): Jurnal Kesehatan
Publisher : Poltekkes Kemenkes Tanjung Karang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26630/jk.v15i1.4332

Dental age estimation is one of the methods in the forensic dental examination. Dental age estimates are often carried out to complete the victim's identity using methods from experts such as Al-Qahtani. There have been many studies related to estimating tooth age using the Al-Qahtani method in normal humans but not in human subjects with special needs, such as children with down syndrome. This study aims to determine the estimated dental age using the Al-Qahtani method in children with down syndrome and to differentiate between chronological age and dental age in children with down syndrome. This research is a cross-sectional study. The research subjects were 13 children with down syndrome who attended special schools in the Jember Regency and met the sample criteria. Tooth age estimation is done by matching the results of panoramic radiographs with the Al-Qahtani Atlas. Data were analyzed using Shapiro-Wilk, Levene's Test, and paired sample T-Test. Estimating dental age using the Al-Qahtani method shows varying dental age values, and there is a delay in the dental age of boys compared to girls with down syndrome. The dental age of all research subjects was delayed from their chronological age. The dental age of children with Down syndrome estimated using the Al-Qahtani method shows an average value of 1 year 2.5 months, and there is a difference between dental age and chronological age with a difference of 1 year seven months. Therefore, adjustments need to be made if the Al-Qahtani method is applied to children with down syndrome.

Komposit Bioactive Glass Berbasis Silika Abu Ampas Tebu dan Gum Mimba Menstimulasi Proses Remineralisasi Permukaan Gigi Indahyani, Didin Erma; Barid, Izzata; Verawati, Verawati
STOMATOGNATIC - Jurnal Kedokteran Gigi Vol 21 No 1 (2024)
Publisher : Fakultas Kedokteran Gigi Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/stoma.v21i1.47331

Dentin hypersensitivity results from exposed dentinal tubules. One of the treatments used is remineralization of dentin and cementum. Bioactive glass (BAG) is a material that can stimulate the remineralization process due to its silica content. Sugarcane bagasse contains high levels of silica and is used as a BAG material. Adding binders and emulsifiers to paste forms increases bonding and stability and makes application easier. Neem gum is a polysaccharide, has emulsifying properties, and can unite two or more ingredients with different properties, increasing the strength of the ingredients. The study aims to analyse the effect of neem gum and bioactive glass composites based on silica bagasse ash on tooth remineralisation. There were three research groups, namely control (BAG paste), treatment A (hyaluronic acid BAG paste) and treatment B (BAG paste, hyaluronic acid and neem gum). The injection paste was applied to the teeth [using cow teeth] on the mesial surface, then soaked in artificial saliva for six days and stored in an incubator at 370C. The remineralization process was observed by forming hydroxycarbonate apatite (HCA) on the tooth root surface using a scoring method based on the Scanning Electron Microscope (SEM) results. CA formation in the BAG, hyaluronic acid and neem gum composite group had a significantly (p<0.05) higher HCA formation score than the group without gum. The control group experienced a washout, so no HCA was formed. BAG composite paste with silica based on bagasse ash and neem gum stimulates the remineralization process marked by the formation of HCA on the tooth root surface.

The value of imbibition and syneresis for dental impression on red seaweed: a laboratory experiment Indahyani, Didin Erma; Barid, Izzata; Anggraeni, Priska Amelia
Padjadjaran Journal of Dentistry Vol 35, No 3 (2023): November 2023
Publisher : Faculty of Dentistry Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol35no3.49071

ABSTRACT Introduction: Alginate is a type of elastic dental impression, which is an irreversible hydrocolloid. The main components in sodium alginate can be obtained naturally through the extraction process from seaweed (Kappaphycus alvarezii). Several physical characteristics must be fulfilled by dental impressions, such as dimensional stability related to imbibition and syneresis. ANSI/ADA specification No.18 regarding the maximum permitted change in alginate impression materials may not exceed 0.5%. This research aimed to analyze the value of imbibition and syneresis on dental impressions of red seaweed extract (K.alvarezii). Methods: The research was conducted using an experimental laboratory method with a post-test-only control group design. There were two groups, namely the control group of commercial dental impressions and the treatment group using dental impressions with the sodium alginate extracted from red seaweed (K.Alvarezii) by the acid method, and the FTIR test was performed to analyze compound sodium alginate. Imbibition and syneresis measurements were carried out using a calliper on a plaster cast model from negative mould casting results, where previously, the mould was soaked in water for imbibition and evaporated for syneresis. Results: Imbibition and syneresis tests showed that the impression material group with sodium alginate from red algae was significantly lower than those with commercial impression materials. It was concluded that the impression material with sodium alginate from red seaweed had an effect on reducing the percentage of syneresis (p≤0.05) and imbibition (p≤0.05) of the impression material. Based on the results of our research, it was found that the alginate impression material extracted from red seaweed (k.alvarezii) had an imbibition value of 0.33% (p≤0.05) and syneresis of 0.28%, (p≤0.05) lower compared to alginate impression materials. commercial alginate impression material. Conclusion: The imbibition and syneresis values of red seaweed alginate show an excessively low value compared to commercial alginates. KEYWORDS dental impression,sodium alginate, Kappaphycus alvarezii, imbibition, syneresis

Elastic Recovery Properties of Alginate Impression Materials Based on Red Algae Kappaphycus alvarezii Indahyani, Didin Erma; Barid, Izzata; Nafisah, Nafisah; Praharani, Depi; Sumono, Agus; Probosari, Niken; Sulistiyani, Sulistiyani
Journal of Dentistry Indonesia Vol. 30, No. 3
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Alginate impression materials in dentistry are used to form details of the oral cavity organs. The alginate content in the impression material functions to form a gel. One source of alginate is algae. Objective: This study aims to determine the recovery elasticity and setting time of alginate impression materials made from red algae K. alvarezii. Methods: The sodium alginate in the composition of this alginate impression material was extracted from red algae K. alvarezii. The alginate impression material was tested for setting time and recovery elasticity and compared with commercial impression material (Hygedent®). Results: The elastic recovery of red algae sodium alginate impression materials was 97.43% which was not significantly different from the commercial impression materials (98.42%). These results showed that this alginate impression material has the same properties as commercial impression materials. In the setting time test, the the red algal alginate impression material was much longer than the commercial impression material, 1.39 m and 3.30 m respectively. Conclusion: It was concluded that the alginate impression material from the red algae K. alvarezii had a long setting time. At the same time, the recovery of elasticity did not differ from that of commercially available alginate impression materials.

Cytotoxicity analysis of alginate impression materials based red seaweed extract on cultured gingival fibroblast cells Praharani, Depi; Barid, Izzata; Indahyani, Didin Erma; Probosari, Niken; Lestari, Sri; Sulistiyani, Sulistiyani; Sumono, Agus
Odonto : Dental Journal Vol 11, No 2 (2024): December 2024
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.11.2.290-297

Background: Alginate is an impression material that is widely used in dentistry. Alginate can actually also be obtained from natural materials such as red seaweed. The impression procedure causes the impression material to come into contact with oral tissues including the gingiva. Ideally, the materials used must also meet requirements such as low toxicity or non-toxicity so that tissue damage does not occur. This study aims to analyze the cytotoxicity of alginate impression materials from red seaweed extract in gingival fibroblast cell. Method: This experimental laboratory design using post-test only control group design. The research groups consisted of: sodium alginate extract group, red seaweed extract-based alginate impression material, positive control and negative control. Cytotoxicity was tested on gingival fibroblast cell cultures and the effect was analyzed using the MTT assay. Exposure to gingival fibroblast cell cultures was differentiated in three time durations: 5 minutes, 10 minutes and 15 minutes. Each time duration was repeated three times. MTT-formazan production is a method used to measure cell viability (living cells). The data obtained were statistically analyzed using two-way ANOVA test and Tukey HSD post hoc test. Result: There was no significant difference in the average cell viability between the red seaweed extract-based alginate impression material group and the negative control group at an exposure duration of 5 minutes, which was more than 90%. Conclusion: The red seaweed extract-based alginate impression material has no toxic effect on gingival fibroblast cells at 5 minutes exposure.

Cytotoxicity analysis of alginate impression materials based red seaweed extract on cultured gingival fibroblast cells Praharani, Depi; Barid, Izzata; Indahyani, Didin Erma; Probosari, Niken; Lestari, Sri; Sulistiyani, Sulistiyani; Sumono, Agus
Odonto : Dental Journal Vol 11, No 2 (2024): December 2024
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.11.2.290-297

Background: Alginate is an impression material that is widely used in dentistry. Alginate can actually also be obtained from natural materials such as red seaweed. The impression procedure causes the impression material to come into contact with oral tissues including the gingiva. Ideally, the materials used must also meet requirements such as low toxicity or non-toxicity so that tissue damage does not occur. This study aims to analyze the cytotoxicity of alginate impression materials from red seaweed extract in gingival fibroblast cell. Method: This experimental laboratory design using post-test only control group design. The research groups consisted of: sodium alginate extract group, red seaweed extract-based alginate impression material, positive control and negative control. Cytotoxicity was tested on gingival fibroblast cell cultures and the effect was analyzed using the MTT assay. Exposure to gingival fibroblast cell cultures was differentiated in three time durations: 5 minutes, 10 minutes and 15 minutes. Each time duration was repeated three times. MTT-formazan production is a method used to measure cell viability (living cells). The data obtained were statistically analyzed using two-way ANOVA test and Tukey HSD post hoc test. Result: There was no significant difference in the average cell viability between the red seaweed extract-based alginate impression material group and the negative control group at an exposure duration of 5 minutes, which was more than 90%. Conclusion: The red seaweed extract-based alginate impression material has no toxic effect on gingival fibroblast cells at 5 minutes exposure.

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