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All Journal Jurnal Ilmu Dasar BERKALA SAINSTEK Pro-Life Jurnal Biodjati JATI EMAS (Jurnal Aplikasi Teknik dan Pengabdian Masyarakat) Journal of Natural Sciences
Winarsa, Rudju
Biology Department, Faculty Of Mathematic And Natural Sciences, Universitas Jember, Jl. Kalimantan 37, Jember, East Java, Indonesia, 68121
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Control & Systems Engineering Earth & Planetary Sciences Education Engineering Environmental Science Immunology & microbiology Mathematics Physics Public Health Other

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Production and Characterization od Xylanase from Actinomyces ATG 70 Using CornCob Xylan Substrate Farrennina, Tasya Preira; Putri, Amelia Fahreza; Arimurti, Sattya; Winarsa, Rudju; Utarti, Esti
Jurnal ILMU DASAR Vol. 26 No. 2 (2025)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jid.v26i2.53702

Abstract

Xilan is a polysaccharide that can be used as a substrate for producing xylanase. Xylan can be obtained from processing xylan-rich agricultural waste such as corn cobs, which contain 12.4% xylan. Xilan can be hydrolyzed into xylose and xylooligosaccharide (XOS) using the enzyme Xilanase. Actinomycetes have dominant xylanolytic species, Actinomyces ATG 70 has semi-qualitative xylanolytic ability with an enzyme activity index of 3.21±0.55 in xylan media. This study was conducted by extracting xylan from corn cobs, rejuvenating Actinomyces ATG 70, preparing the inoculum, creating a standard curve, producing xylanase with pH optimization of the medium, testing xylanase activity, characterizing the pH and temperature of crude xylanase, and identifying the morphology and biochemical characteristics of Actinomyces ATG 70. Optimization of the pH of the xylanase production medium was achieved at pH 8, with xylanase activity of 10.07±0.13 U/mL. The crude xylanase was characterized for pH and temperature effects, and the optimal pH for crude xylanase was found to be pH 6 with xylanase activity of 7.64±0.66 U/mL, and the optimal temperature was 50°C with xylanase activity of 11.17±0.33 U/mL. The identification results showed that Actinomyces ATG 70 belongs to Gram-positive bacteria, with a positive catalase test, white colonies, cream-colored aerial mycelium, and cream-colored pigmentation. The spore structure consists of long chains of conidia, streptococcus-shaped cells, and can form aerial mycelium, thus the isolate belongs to the genus Streptomyces.
Co-Authors Agus Subekti Alya, Fathia Anisa Azhari, Fitri Esti Utarti Farah Salma Elida Farrennina, Tasya Preira Hidayat Teguh Wiyono Imron Rosyidi, Moh. Kahar Muzakhar Khofiya, Zunairoh Nidaan Khofiya, Zunairoh Nidaan Kusumah, Maulana S. Ningsih, Tutus Ervian Paramita Pratiwi Purwatiningsih Purwatiningsih Purwatiningsih Purwatiningsih Putri, Amelia Fahreza Ramdhan Putrasetya Rasit, Abdur S Siswanto S. Siswanto Sattya Arimurti Siswoyo Siswoyo Sutoyo Sutoyo