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PRODUKSI GUM ARABIC BALURAN SEBAGAI PENDUKUNG PENGEMBANGAN WISATA KAMPUNG BANTENG DI KARANG TEKOK SEBAGAI WILAYAH PENYANGGA TN BALURAN Kusumah, Maulana S.; Wiyono, Hidayat Teguh; Subekti, Agus; Muzakhar, Kahar; Winarsa, Rudju
JATI EMAS (Jurnal Aplikasi Teknik dan Pengabdian Masyarakat) Vol 4 No 1 (2020): Jati Emas (Jurnal Aplikasi Teknik dan Pengabdian Masyarakat)
Publisher : Dewan Pimpinan Daerah (DPD) Forum Dosen Indonesia JATIM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36339/je.v4i1.272

This article is the result of PPDM (Mitra Desa Service Program) about Baluran bos javanicus (Banteng) village tourism. Development of bull village tourism is an effort to solve the problem of wild grazing in Bunaken National Park. In the event of Banteng Village Tourism, it is necessary to support tourism, namely creative industries, agro-tourism, and NTFP production (non-timber forest products). One of the NTFPs that is relied upon is Arabic gum. Currently, cattle breeders in the banteng village area have been able to produce Arabic gum as a result of the introduction of tapping technology by the 2019 PPDM team. The dedication method is in the form of dissemination and field practice. Three groups representing breeders were trained to tap acacia gum through a drilling method combined with ethephon induction as GIS. One week after application, the group begins harvesting gum and submits the results to the group leader. Then the group leader sends the results to the Cooperative in Pondok Pesantren Assalam, Sumberanyar, Banyuputih Situbondo. The amount of Baluran Arabic gum that was collected by the group for three months reached 143.9 kg. This service activity concludes that the strength in producing Baluran Arabic gum is significant in improving the welfare of breeders in supporting the maintenance and retention of a Banteng.

FREKUENSI ALEL GOLONGAN DARAH SISTEM A-B-O PADA POPULASI SUKU OSING DESA KEMIREN KABUPATEN BANYUWANGI Amania, Novita; Wiyono, Hidayat Teguh; Oktarianti, Rike
BIOMA Vol 5, No 1 (2020): BIOMA : JURNAL BIOLOGI DAN PEMBELAJARAN BIOLOGI
Publisher : Universitas Muhammadiyah Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32528/bioma.v5i1.3680

Suku Osing di desa Kemiren, Banyuwangi memiliki tradisi perkawinan upek-upekkan, yaitu perkawinan antar kerabat. Perkawinan ini termasuk dalam perkawinan endogami yang menyebabkan menurunnya variasi genetik. Penelitian ini bertujuan untuk mengetahui proporsi fenotip, frekuensi alel, dan kesetimbangan genetic Hardy-Weinberg golongan darah ABO pada populasi suku Osing di Desa Kemiren. Pengambilan sampel dilakukan secara acak. Identifikasi golongan darah dilakukan dengan metode slide test. Analisis penelitian menggunakan uji Chi-square. Hasil penelitian menunjukkan golongan darah pada populasi suku Osing di Desa Kemiren sebagai berikut: golongan darah O (45,95%), A (23,99%), B (21,97%), dan AB (8,09%). Frekuensi alel masing-masing adalah alel IA (0,16), frekuensi alel IB (0,16), dan frekuensi alel i (0,68). Hasil pengujian kesetimbangan genetik Hardy-Weinberg dengan uji Chi-square menunjukkan tidak ada penyimpangan yang signifikan.

Efek Ekstrak Daun Seledri (Apium graveolens L.) Terhadap Struktur Histologi Ginjal Tikus (Rattus norvegi Nana Zaimatul Husna; Mahriani Mahriani; Hidayat Teguh Wiyono
Metamorfosa: Journal of Biological Sciences Vol 8 No 1 (2021)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2021.v08.i01.p10

The kidneys are an important excretory organ for removing the rest of the body's metabolism. Kidney stones can affect kidney function. Celery contains flavonoid compounds that can improve the histological structure of the kidneys. This study aims to determine the effect of celery extract on the histological structure of the kidneys, namely the diameter of the glomerulus and tubule with protein deposits. This research is experimental with completely randomized design (CRD). The treatment group was divided into 4 groups, namely, negative control (K-), rats were only given food and drink; positive control (K+), rats induced Ethylene glycol (EG) 0.75% + Ammonium Chloride (AK) 2%, dose 1 (D1), rats induced EG 0.75% + AK 2% and celery extract 100 mg / kg, dose 2 (D2), rats induced EG 0.75% + AK 2% and celery extract 200 mg / kgBB. Celery extract was given for 14 days by gavage. Celery extract at doses of 100 and 200 mg / kgBB were able to improve the histological structure of the rats' kidneys induced by EG 0.75% and AK 2%. Keywords: celery, glomerular diameter, protein deposits

Distribusi dan Frekuensi Alel Golongan Darah Sistem ABO dan Rhesus pada Penduduk Pulau Gili Ketapang Probolinggo Kirana Eka Rezki; Rike Oktarianti; Hidayat Teguh Wiyono; Purwatiningsih Purwatiningsih
Jurnal Ilmiah Biosaintropis (Bioscience -Tropic) Vol 7 No 1 (2021): Agustus 2021
Publisher : Fakultas Matematika & Ilmu Pengetahuan Alam - Universitas Islam Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (461.049 KB)

Sistem penggolongan darah pada manusia yang banyak dikenal adalah sistem ABO dan rhesus. Tujuan penelitian ini adalah Mengetahui distribusi golongan darah sistem ABO dan rhesus serta frekuensi alel pada populasi penduduk pulau Gili Ketapang, kabupaten Probolinggo. Pengambilan sampel dilakukan secara acak dengan cara dilakukan pengundian jumlah sampel yang diambil sebanyak 384 jiwa. Identifikasi golongan darah sistem ABO pada penelitian ini dilakukan dengan metode slide dengan prinsip antigen (aglutinogen) yang direaksikan dengan antibodi (aglutinin). Hasil penelitian menunjukkan distribusi golongan darah sistem ABO dan Rhesus O+ (46,61%), B+ (24,22%), A+ (23,18%), dan AB+ (5,99%). Frekuensi alel IA(0,16), frekuensi alel IB (0,16), frekuensi alel i (0,68), dan frekuensi alel rhesus positif (Rh+) adalah 1

Prevalensi Buta Warna pada Siswa Sekolah Dasar di Pulau Gili Ketapang Kabupaten Probolinggo Nailatur Rifdah; Rike Oktarianti; Hidayat Teguh Wiyono; Asmoro Lelono; Husnatun Nihayah
Jurnal Ilmiah Biosaintropis (Bioscience -Tropic) Vol 7 No 2 (2022): Januari 2022
Publisher : Fakultas Matematika & Ilmu Pengetahuan Alam - Universitas Islam Malang

Buta warna merupakan salah satu kendala dalam proses pembelajaan setiap siswa tingkat dasar yang harus diketahui sejak dini. Buta warna utamanya disebabkan oleh faktor genetik. Buta warna merupakan kelainan genetik sex linkage pada kromosom X. Tujuan dari penelitian ini yaitu untuk mengetahui prevalensi penderita buta warna, frekuensi alel buta warna dan normal, dan mengetahui diagram silsilah keluarga penderita buta warna. Pengujian buta warna menggunakan metode Ishihara. Siswa yang diambil sebagai sampel yaitu 255 siswa yang telah dipilih secara acak, dengan rentang usia 8-12 tahun. Pengambilan sampel sesuai dengan prosedur pada Ethical clearance nomor 1224/UN25.8/KEPK/DL/2021. Hasil penelitian menunjukkan bahwa prevalensi penderita buta warna sebesar 3.14% dan prevalensi rang normal sebesar 96.86%. Frekuensi alel buta warna dan alel normal berturut-turut adalah 0,023 dan 0,977. Diagram silsilah pada keluarga penderita buta warna menunjukkan bahwa pola pewarisan buta warna adalah pola pewarisan bersilang (criss cross inheritance).

Relationship Bali Cattle (Bos sondaicus Muller) and Banteng (Bos bibos d'alton) Approach Through The Craniometric Arya Mahdi; Hidayat Teguh Wiyono; Suratno Suratno
Jurnal ILMU DASAR Vol 14 No 2 (2013)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Relationship can be seen from the similarity of the cow and bull. One method to find out about the phylogenetic relationship between species is by using cranial morphometry (craniometrics). The purpose of this research was to identify morphological cranium Bali cattle , banteng baluran , and banteng merubetiri based on the parameters measured .This research was conducted in August and ending in October 2013 , held at the National Park Baluran and Merubetiri National Park. The design of this research is descriptive quantitative research , using data analysis kalster ( cluster analysis). Parameters of this research using 15 indicators that have been established in accordance with the research Hayashi , 1982. Materials used in this study are adult male cranium Bali cattle obtained from abattoir ( Slaughterhouse ) Denpasar in January to July 2013 a total of six specimens . Cranium of an adult male bull that used a total of six specimens . Five specimens were collected from the National Park consisting Baluran ( two cranium that is banteng baluran 4 and 5 in 1992 ) and the ( three cranium is banteng baluran1, banteng baluran 2 , and banteng baluran 3 in the 2012-2013 ) . Cranium specimen obtained from the sixth bull collection Merubetiri National Park . Measuring instruments used in this study is the large calipers with a precision level of 0.01 cm . The results of this study showed that the cranium cluster of Bali cattle with coefficient range of 0.016 to 0.042 in contrast to the first cluster is bull cranium specimen glaze 1 bull , bull glaze 2 , 3 baluran bull , and the bull merubetiri 1 , with a coefficient value of 0.047 to 0.161 range . Bali cattle cranium differ significantly with cluster cranium second bull is a bull glaze glaze glaze bull 4 and 5 with the coefficient of 0.162 to 0.251 range. Merubetiri bull glaze and form two clusters , the first cluster bull bull glaze consisting of 1 , 2 bull glaze , glaze bull bull merubetiri 3 and 1 , while the second consists of a cluster bull bull bull glaze glaze 4 and 5 . Turns bull glaze specimens collected in 1992 in contrast to the bull glaze specimens collected in 2012 and 2013. Bull glaze specimens collected in 2012 and 2013, closer to the bull merubetiri specimens . And the bull specimens closer distance coefficient with Bali cattle. The conclusion of this study is the overall specimen Bali cattle entered in a cluster , different from the first cluster and cluster bull bull both . The first cluster by cluster bull bull both are in different clusters. Keywords: cluster analysis , relationship , craniometric , bali cattle and banteng.

Profile of Interleukin-4 and Interferon-γ of Balb/c Mice after Salivary Gland Extract of Anopheles sundaicus s.l. Vaccination and Infected by Plasmodium berghei-ANKA Ali Machrus; Adrial Adrial; Yunita Armiyanti; Hidayat Teguh Wiyono; Kartika Senjarini
Jurnal ILMU DASAR Vol 15 No 2 (2014)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Malaria infection is initiated when sporozoites are inoculated into a vertebrate host via the salivary glands of an Anopheles mosquito. During Anopheles bite, the salivary glands release components that include vasomodulator and immunomodulators. The salivary components of vectors have important role in transmission of pathogen. Therefore, if these components were injected repeatedly into a vertebrate host, it can stimulate host immune system and inhibit the transmission of the pathogen into the host. This could be observed the increasing level of IFN-γ and decreasing level of IL-4 in mice model of malaria after vaccination with salivary gland ekstract (SGE) from An sundaicus s.l. It has also been proven that this mechanisms was related with pathogen of malaria. This was supported by the reduction of parasitemie rate in those mice model after infection by P. Berghei. Keywords: An sundaicus s.l., IFN-γ, IL-4,immunomodulators, salivary gland , TBV

FREKUENSI ALEL GOLONGAN DARAH SISTEM A-B-O PADA POPULASI SUKU OSING DESA KEMIREN KABUPATEN BANYUWANGI Novita Amania; Hidayat Teguh Wiyono; Rike Oktarianti
BIOMA Vol 5, No 1 (2020): BIOMA : JURNAL BIOLOGI DAN PEMBELAJARAN BIOLOGI
Publisher : Universitas Muhammadiyah Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32528/bioma.v5i1.3680

Suku Osing di desa Kemiren, Banyuwangi memiliki tradisi perkawinan upek-upekkan, yaitu perkawinan antar kerabat. Perkawinan ini termasuk dalam perkawinan endogami yang menyebabkan menurunnya variasi genetik. Penelitian ini bertujuan untuk mengetahui proporsi fenotip, frekuensi alel, dan kesetimbangan genetic Hardy-Weinberg golongan darah ABO pada populasi suku Osing di Desa Kemiren. Pengambilan sampel dilakukan secara acak. Identifikasi golongan darah dilakukan dengan metode slide test. Analisis penelitian menggunakan uji Chi-square. Hasil penelitian menunjukkan golongan darah pada populasi suku Osing di Desa Kemiren sebagai berikut: golongan darah O (45,95%), A (23,99%), B (21,97%), dan AB (8,09%). Frekuensi alel masing-masing adalah alel IA (0,16), frekuensi alel IB (0,16), dan frekuensi alel i (0,68). Hasil pengujian kesetimbangan genetik Hardy-Weinberg dengan uji Chi-square menunjukkan tidak ada penyimpangan yang signifikan.

An Extracellular Pectinase from ISH16 Bacteria Isolated Induced by Coffee Pulp Waste Substrate Kahar Muzakhar; Farah Salma Elida; Ramdhan Putrasetya; Siswoyo Siswoyo; Rudju Winarsa; Hidayat Teguh Wiyono
Jurnal Biodjati Vol 7, No 2 (2022): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v7i2.20279

An α-1,4-glycosidic bonds galactoses pectin, mainly composed of a D-galacturonic acid chain, are important biomaterial widely used in industries. Utilizing this material, a bioprocess, including the biocatalysis pectinase, is often needed. Pectinase production was optimized in 7 days SSF at 37°C, and the pectinase activities were daily measured by the method of Somogy-Nelson. The optimum pectinase production was 0.166 U/ml on the fourth day SSF. Purification using open column ion exchange chromatography DEAE cellulose DE-52 resulted in 1030.9 folds of pectinase purity with a yield of 25.9%. The enzyme was at optimal activity at pH six and attended stable in the pH range of 5.5-8, while optimal activity at a temperature of 50°C and was stable in the range of 30-45°C. The pectinase activity increased by 120% with the addition of 10 mM Mg2+, and 95% retained when 10 mM Ca2+ was added. The presence of 10 mM Na+, K+, and Fe2+ resulted in a slight effect of activity at 85%, 83%, and 78%. However, it was strongly inhibited by 10 mM Al3+ and retained 25%. Based on the results above, the microbial utilization of coffee pulp waste by ISH16 bacteria pectinolytic is one opportunity to produce valuable pectinase with low-cost production, so comprehensive examination in large-scale production is needed too. In this paper, all research detail steps were described.

Prevalensi Kemampuan Mengecap Phenylthiocarbamide (PTC) Pada Penduduk Pulau Gili Ketapang Kabupaten Probolinggo Rike Oktarianti; Siti Agustina Wulandari; Hidayat Teguh Wiyono; Mahriani Mahriani
Metamorfosa: Journal of Biological Sciences Vol 10 No 1 (2023)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2023.v10.i01.p07

The taste of sensitivity of Phenylthiocarbamide (PTC), is autosomal dominant trait inherited. The taster controled by dominant allele while the non-taster determinated by recessive allele. The purpose of this study was to determine the prevalence and frequency of taster and non-taster alleles for PTC, as well as non-taster inheritance patterns at the population of Gili Ketapang island, Probolinggo district. Determination of the sample or respondents was carried out by randomly, total sample were 384 people, aged from 15-30 years. Detection of the taste sensitivity PTC was ask to respondents to taste the PTC solution. The PTC solution used was from the lowest concentration of 0.32mg/L (P13) to the highest concentration of 1300 mg/L (P1). The results showed that the prevalence of taster and non-taster were 96.88% and 3.12%, respectively. The frequency of taster (T) and non-taster alleles was 0.83 and non-taster was 0.17. The pedigree analysis of non-taster showed that non-taster individuals were born from heterozygous taster pairs (Tt) or from marriages between heterozygous taster individual (Tt) with non-tasters (tt). Keyword: Phenylthiocarbamide (PTC), taster, non taster

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