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Spectrophotometric Analysis of Caffeine Content in Coffee Mistletoe (Dendrophthoe petandra L.) Apriliana, Gepsa; Indarti, Dwi; Muflihah, Yeni Maulidah
Indonesian Chimica Letters Vol. 2 No. 2 (2023)
Publisher : Department of Chemistry, Faculty of Mathematics and Natural Sciences, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/icl.v2i2.5606

Abstract

Caffeine, a well-known alkaloid presents in plants such as tea, coffee, and kola nuts, was thoroughly analyzed in coffee mistletoe. Our study successfully extracted caffeine using dichloromethane and chloroform, incorporating qualitative testing and robust method validation with a visible spectrophotometer. Notably, dichloromethane yielded the highest caffeine extract at 0.042 grams. The qualitative tests distinctly confirmed the presence of caffeine, evidenced by a color change with Parry's reagent and a maximum absorption wavelength of 273 nm. We also pinpointed the caffeine complex’s maximum wavelength at 640 nm, further validating our findings against a standard solution. Our method validation showed impressive linearity (r = 0.9974), with limits of detection at 1.81 ppm and quantitation at 6.02 ppm. The accuracy ranged between 90.0% and 97.5%, while the precision values were consistently around 98%. Additionally, we determined the water content of the coffee mistletoe leaf powder to be 8.733%. This comprehensive analysis establishes a strong foundation for the caffeine content in coffee mistletoe.
The Effect of Concentrated Seawater Salinity on Soybean Protein Coagulation in Tofu Production Rohmawati, Manis; Muflihah, Yeni Maulidah; Handayani, Wuryanti; Asnawati; Mulyono, Tri
Indonesian Chimica Letters Vol. 4 No. 1 (2025)
Publisher : Department of Chemistry, Faculty of Mathematics and Natural Sciences, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/icl.v4i1.5885

Abstract

Seawater, which has a salinity of 35 ‰, contains essential ions such as chloride, sodium, sulfate, magnesium, and calcium. These ions play a crucial role in the coagulation of proteins. Salt-based coagulants are among the oldest and most commonly used in tofu production. Additionally, several metal cations exhibit similar coagulating effects on soybean proteins. Tofu can be produced by adding salt coagulants, like calcium sulfate (commonly known as tofu stone) and seawater extract. The seawater extract was obtained from seawater through evaporation in three distinct ponds, with varying evaporation times that can lead to differences in salinity and density. In this experiment, we used coagulants from these three ponds, labeled A, B, and C. Coagulant C, derived from the pond with the longest evaporation time, has the highest salinity of 310 ‰ and a density of 1.220 g/cm³. The mass of the tofu produced shows a consistent pattern among coagulants A, B, and C: an initial increase followed by a decrease, which is influenced by the salting-out and salting-in processes. When used at a volume of 15 mL, Coagulant C yielded the highest mass at 179.426 grams and the lowest water content at 71.152%, demonstrating its effectiveness in protein coagulation.