Article Per Year (5 Year)
p-Index From 2020 - 2025
2.064
P-Index
This Author published in this journals
All Journal Jurnal Ilmu Dasar BERKALA SAINSTEK UNEJ e-Proceeding Jurnal Kimia Riset Jurnal Biodjati Indonesian Chimica Letters InSTEM Prosiding Seminar Nasional Pengabdian kepada Masyarakat (SINAPMAS) Journal of Bio-molecule Research And Engineering
Wuryanti Handayani
Jurusan Kimia, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Jember
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Control & Systems Engineering Earth & Planetary Sciences Education Immunology & microbiology Mathematics Physics Other

Published : 19 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 19 Documents
Search

 1   2 
Optimization pH of Enzymatic Hydrolysis of Endo-1,4-β-Xylanase for Xylooligosaccharides Production Ratnadewi, Anak Agung Istri; Kurniawan, Andika Ade; Handayani, Wuryanti
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UNEJ e-Proceeding

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Xylooligosaccharides (XOS) with polymerisation degree between 2 till 10 monomer  have prebiotic effect for better digestion system. In this research, production of XOS was performed by enzymatic hydrolysis of xylan with endo-1,4-β-xylanases enzyme. Endo 1,4-β-xylanases enzyme was aqcuired from Bacillus sp. isolated from termite’s abdominal.Meanwhile oat xylan was used as substrate. Optimal condition of enzymatic hydrolysis was evaluated at pH: 4, 5, 6, and 7 with incubation time from 5 to 20 h.pH 5 was optimum pH to produce XOS from 0.8 % xylan oat at 40 oC.The hydrolysis product purified and analyzed by thin layer chromatographyyielding spot of xylobiose, xylotriose, xilotetraose and xilopentaose. Further analysis by HPLC indicated dominant xilopentaosa X5 (3522 ppm) among  the other XOS   X2 (14 ppm), X3 (43 ppm) and X4 (15 ppm). Keywords: Xylooligosaccharides, endo-1,4-β- endoxylanase
Screening Fitokimia dan Studi Aktivitas Ekstrak Daun Sintok (Cinnamomum sintoc Bl.) Sebagai Antioksidan dan Antihiperlipidemia Kumalasari, Ardine; Handayani, Wuryanti; Siswoyo, Tri Agus
BERKALA SAINSTEK Vol 7 No 1 (2019)
Publisher : Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bst.v7i1.9916

Abstract

Cinnamomum sintoc Bl. merupakan tanaman yang memiliki kandungan minyak atsiri di kulit batangnya yang digunakan untuk antioksidan, antihiperlipidemia dan lain-lain. Dari penelitian ini, senyawa metabolit sekunder dari daun sintok (kadar air 54,7%±0,69) diekstrak dengan metanol dan dianalisis dengan reagen spesifik. Daun sintok diekstraksi secara maserasi bertingkat dengan meningkatkan kepolaran pelarut, yaitu n-heksana, etil asetat, metanol. Uji antioksidan dan antihiperlipidemia dilakukan pada setiap ekstrak(HS, ES, MS) menggunakan standar asam galat equivalent. Total fenolik dan total flavonoid dihitung menggunakan kurva standar asam galat dan kuersetin, hasil total fenolik dari setiap ekstrak antara lain HS (39,23±2,79 mg AGE/g); ES (110,77±2,37 mg AGE/g); dan MS (283,63±3,96 mg AGE/g). Aktivitas antioksidan pada ekstrak ditentukan dengan kemampuan ekstrak untuk meredam DPPH sedangkan aktivitas antihiperlipidemia ekstrak ditentukan dengan kemampuan ekstrak untuk menghambat kinerja lipase. Potensi ekstrak daun sintok terhadap antioksidan cukup tinggi hanya untuk ekstrak MS, sedangkan potensi terhadap antihiperlipidemia untuk semua jenis ekstrak. Kata Kunci: Cinnamomum sintoc Bl., fitokimia, maserasi, fenolik, antioksidan, antihiperlipidemia.
OPTIMASI KONSENTRASI SUBSTRAT XILAN AMPAS TAHU TERHADAP ENDO-Β-1,4-D-XYLANASE UNTUK MEMPRODUKSI XILOOLIGOSAKARIDA Anak Agung Istri Ratnadewi; Wuryanti Handayani; Siti Nur Avida
Jurnal Kimia Riset Vol. 1 No. 2 (2016): Desember
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (496.779 KB) | DOI: 10.20473/jkr.v1i2.3084

Abstract

AbstrakAmpas tahu merupakan limbah samping dari proses pengolahan tahu dan susu kedelai. Ampas tahu berpotensi sebagai sumber xilan. Xilan digunakan sebagai substrat endo-β-1,4-D-xilanase untuk menghasilkan xilooligosakarida. Penelitian ini digunakan xilan ampas yang telah dihilangkan lemak dan protein tanpa penghilangan lignin (X1nD). Xilan ampas tahu tanpa penghilangan lemak dan protein tetapi dilakukan penghilangan lignin (X2D). Enzim yang digunakan adalah endo-β-1,4-D-xilanase dari isolat Bacillus sp. asal abdomen rayap. Optimasi variasi konsentrasi substrat bertujuan untuk mengetahui konsentrasi optimum dalam menghasilkan xilooligosakarida. Produk hidrolisis yang dihasilkan dianalisis menggunakan metode Miller untuk mengetahui total gula pereduksi. Produk hidrolisis konsentrasi optimum dianalisis menggunakan Kromatografi Lapis Tipis (KLT) untuk mengetahui komponen penyusun xilooligosakarida. Substrat X1nD dan X2D optimum pada konsentrasi 6% dan 5% dengan total gula pereduksi sebesar 0,196 mg/ml dan 0,211 mg/ml. Komponen penyusun xilooligosakarida ampas tahu berupa xilotriosa (X3), xilotetraosa (X4), dan xilopentaosa (X5).Kata Kunci: Ampas tahu, endo-β-1,4-D-xilanase, xilan, xilooligosakarida. AbstractOkara is a waste byproduct of the processing of tofu and soy milk. Okara potential as a source of xylan. Xylan is used as the substrate endo-1,4-β-D-xylanase to produce xyloologosaccharide. This study used okara xylan had eliminated fat and protein without removal of lignin (X1nD). Okara xylan out without the removal of fat and protein but do removal of lignin (X2D). The enzyme used is endo-1,4-β-D-xylanase of isolates of Bacillus sp. From abdominal termites. Optimization of substrate concentration variation aims to determine the optimum concentration in generating xyloologosaccharide. Hydrolysis products were analyzed using Miller method to determine total reducing sugars. The optimum concentration of hydrolysis products were analyzed using Thin Layer Chromatography (TLC) to determine the components of xyloologosaccharide. X1nD and X2D optimum substrate at a concentration of 6% and 5% to the total reducing sugars of 0.196 mg/ml and 0.211 mg/ml. Xyloologosaccharide of okara components of the pulp out the form xylotriose (X3), xylotetraose (X4), and xylopentaose (X5).Keywords: Okara, endo-1,4-β-D-xylanase, xylan, xyloologosaccharide
Production and Characterization of Enzyme β-Endoxylanase from Bacteria of Termite-intestinal System A. A. Istri Ratnawati; Wuryanti Handayani; Ni Nyoman Tri Puspaningsih
Jurnal ILMU DASAR Vol 8 No 2 (2007)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (111.567 KB)

Abstract

A mesophile bacteria isolated from intestinal systems of local-soil termite, produced an extracellular β-endoxylanase upon growth on xylan. Optimum production of the enzyme was found in media containing oatspelt xylan at 37oC after sixth hours. The activities of its β-endoxylanase on oat-spelt xylan was investigated. It had an optimum pH and temperature, 5.0 and 40o C, respectively. However, pH stability occurred between 5.08.0. The enzyme was stable at 40o C for four hours and possessed a half life of four hours. β-endoxylanase had an apparent molecular mass of 45.000 to 66.200 Dalton as determined by SDS-PAGE. Analysis of zymogram using SDS-Xylan-PAGE indicated that enzymes could degrade oat-spelt xylan as substrates.
Inovasi Penambahan Prebiotik Xilooligosakarida Dan Peningkatan Pemasaran Pada Produk Usaha Kecil Penghasil Kue Pia di Kota Jember Anak Agung Istri Ratnadewi; Wuryanti Handayani; Sudarko Sudarko; Nyoman Gede Krishnabudi
Prosiding Seminar Nasional Pengabdian kepada Masyarakat (SINAPMAS) Perguruan Tinggi Mengabdi: Berkarya dan Berinovasi Untuk Membangun Masyarakat Semakin Tangguh di Mas
Publisher : Prosiding Seminar Nasional Pengabdian kepada Masyarakat (SINAPMAS)

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Program Pengabdian Berbasis Penelitian (PPBP) ini untuk memberdayakan ekonomi usaha pembuatan kue khas Jember. UKM yang dilibatkan adalah UKM produk kue Pia merk “Syam”, usaha ini tidak jauh dari Universitas Jember berkisar 7 Km. Merk kue Pia “Syam” merupakan salah satu usaha kue home industri yang melibatkan ibu-ibu rumah tangga di sekitarnya. Pemasaran masih disekitar toko-toko kue disekitar kota Jember. Usaha kue ini meskipun sudah berjalan lama di mulai usaha sejak 2006, namun masih relatif tradisional sehingga memerlukan pengembangan untuk dapat bersaing untuk menghasilkan produk kue yang berkualitas dan dapat bersaing di pasaran. Permasalahan yang muncul adalah (1) bagaimana meningkatkan kualitas produk sehingga mampu menjadi produk andalan yang dicari pengguna, dengan produk kue yang mempunyai nilai qisi dan kualitas yang terjamin, (2) bagaimana meningkatkan pemasaran produk kue Pia. Berdasarkan permasalahan tersebut maka solusi yang dapat diterapkan adalah membantu mitra bagaimana kualitas produk ditingkatkan menggunakan konsep sains dan teknologi dari praktisi perguruan tinggi. Dalam kegiatan pengabdian ini Kelompok KeRis Enzim akan membantu mengatasi masalah pertama dengan meningkatkan kualitas kue dengan memberikan inovasi pada produk kue dengan penambahan bahan prebiotik xilooligosakarida (XOS) melalui teknologi enzimatis. Kandungan prebiotik XOS diperoleh dari penambahan enzim xilanase pada adonan kulit kue Pia yang berbasis bahan tepung. Secara in-situ enzim ini akan menghidrolisis substrat pada adonat kulit kue Pia menghasilkan prebiotik XOS yang sangat bermanfaat untuk kesehatan. Enzim xilanase merupakan hasil penelitian Kelompok KeRis Enzim telah diuji stabilitas dan kemampuannya menghasilkan produk prebiotik XOS dan enzim ini telah mempunyai No paten IDP 000075875. Prebiotik merupakan oligosakarida yang tidak terdigesti dan menguntungkan untuk pertumbuhan bakteri yang baik dalam sistem pencernaan, dapat membantu penyerapan nutrisi dan melancarkan metabolism kolesterol dalam tubuh. Dengan kandungan prebiotik XOS, produk kue yang dihasilkan akan mempunyai nilai lebih dibanding produk lain, sehingga akan mempunyai ciri khas yang berbeda dengan produk yang sudah ada dipasaran. Pada permasalahan kedua Kelompok Keris Enzim membantu meningkatkan pemasaran dengan mengenalkan metode pemasaran modern berbasis IT sehingga produk mudah dikenal dan mudah dipesan. Bentuk kegiatan yang akan kami lakukan melalui pendampingan intensif pembuatan produk dengan penambahan bahan prebiotik XOS dengan teknik enzimatis. Kegiatan pemasaran usaha ini masih konvensional yaitu dengan menawarkan produk kepada kenalan dan orang terdekat melalui Whatsapp dan Facebook pribadi pemilik usaha saja. Hal ini membuat cakupan pemasaran dan produktivitas usaha rumah tangga Kue Pia “Syam” Jember kurang dapat berkembang dan terbilang belum dapat bersaing di pasar. Perlu adanya pelatihan dan pendampingan untuk membantu kegiatan promosi, pemasaran serta branding produk kue pia dengan memanfaatkan fasilitas akun sosial media dan aplikasi lainnya untuk dapat menjawab peluang pasar yang lebih luas secara online seperti misalnya terafiliasi dengan jasa layan antar produk semacam Gofood, Grabfood atau jasa layan antar lokal sejenis di area Jember dan sekitarnya. Selain itu untuk mengembangkan sayap agar permintaan tidak sebatas di Jember saja, maka perlu juga memasukkan unsur pemasaran digital atau ecommerce semacam Shopee, Bukalapak, Tokopedia dan lain-lain.Kata kunci : UKM Syam, Kue Pia, prebiotik, xilooligosakarida
The Application of Slow Sand Filtration Technology for Improving Water Quality at Taman Gading Housing Area, Kaliwates District, Jember Muflihah, Yeni Maulidah; Utomo, Denny Trias; Handayani, Wuryanti; Sulistiyo, Yudi Aris; Andarini, Novita; Asnawati, Asnawati; Indarti, Dwi
Jurnal Inovasi Sains dan Teknologi untuk Masyarakat Vol. 1 No. 2 (2023): November
Publisher : Faculty of Mathematics and Natural Sciences, University of Jember. Jl. Kalimantan No.37, Krajan Timur, Jemberlor, Kec. Sumbersari, Jember Regency, East Java 68121

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/instem.v1i2.470

Abstract

Water is a basic need for all living organisms. Water quality implies living quality. Several parameters influencing water quality include minerals, dissolved organic substances, and turbidity. The water consumed must meet proven water quality standards. However, water quality still needs to be improved in several areas before consumption. The well water in residential areas placed on rice fields generally needs to meet the requirements for consumption. Many Taman Gading housing complex residents, especially in RW 036 RT 006, still use the well water to meet their daily needs. The well water quality in this area tends to be smelly and somewhat misty. Proper treatment is needed to improve the quality of well water in this area to reduce the negative impact on the population's health in the long term. This training focused on well water purification and filtration using slow sand filtration technology (SPL) to improve water quality. This method uses activated charcoal, sand, gauze, and pumice as filter media in specific proportions. The use of this method is effective and efficient in reducing the turbidity level of the sample water to the threshold for drinking water (<500 ppm), with a neutral pH (close to 7.00). This technology is effortless for residents to use and apply independently at a relatively low cost.
Determination Kinetic Parameters of Endo-β-1,4-D-Xylanase from Abdomenal Termites with Xylan Oat and Birchwood Istri Ratnadewi, Anak Agung; Linda Faiqotul Himmah; Tri Mulyono; Wuryanti Handayani; NG. Krishnabudi; Sudarko
Indonesian Chimica Letters Vol. 1 No. 1 (2022)
Publisher : Department of Chemistry, Faculty of Mathematics and Natural Sciences, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (564.421 KB) | DOI: 10.19184/icl.v1i1.3

Abstract

Parameters kinetic (KM, VMax, and kCat) endo-β-1,4-D-xylanase under optimum conditions with oat spelt xylan and birchwood substrate have been investigated in this study. Hydrolysis of endo-β-1,4-D-xylanase using variation of substrate concentration (b/v) ranging from 0.2 to 1.2%. Variation of incubation time is up to 20 hours with 4 hours interval at the optimum temperature of the enzyme, 40°C. The results obtained from this study were the KM of endo-β-1,4-D-xylanase for oat spelt xylan and birchwood were 4.10 mg/ml and 0.681 mg/ml, respectively. VMax values, and kCat for oat spelt xylan substrate of 0.28 mg/ml.jam and 1.7 x 10-3 s-1. While VMax, and kCat for birchwood substrate that is 0.117 mg/U/jam and 7 x 10-4 s-1. From the results of this study we found that endo-β-1,4-D-xylanase can hydrolaze substrates which have differences solubility.
Optimization of Centrifugation Speed and pH in Extraction of Uricase Enzyme from Goat Liver Handayani, Wuryanti; Esti Utarti; Riki Juni Krismiadi; AA. Istri Ratnadewi
Indonesian Chimica Letters Vol. 1 No. 2 (2022)
Publisher : Department of Chemistry, Faculty of Mathematics and Natural Sciences, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (719.421 KB) | DOI: 10.19184/icl.v1i2.203

Abstract

The human body does not have an enzyme that can break down uric acid, so the accumulation of uric acid can cause disease. This problem can be overcome by uricolytic therapy by utilizing the activity of the uricase enzyme. In this study, the uricase enzyme was extracted from goat liver by optimizing the centrifugation speed and the extraction pH. The purpose of the optimization is to get maximum uricase activity. Uricase extraction to optimize centrifugation speed using borate buffer pH 8.5 then centrifuged at 7.000; 9.000; 11.000; 13.000 and 15.000 rpm with a temperature of 4oC. Furthermore, pH optimization was carried out using pH buffers 6, 7, 8, 9, 10 and 11 by centrifuging the optimum speed obtained. The crude extract obtained was further tested for its enzyme activity . The results showed that the highest uricase activity was achieved if the extraction was carried out at pH 8 using centrifugation at an optimum speed of 13,000 rpm. The higher uricase activity indicates that the extracted uricase concentration is increasing.
Optimum pH Buffer of Phosphate and Carbonate on The Crude Extraction of Uricase Enzyme from Goat Liver: Uricase Wuryanti Handayani; Leyla Novita Brigiyanti; Sudarko; Istri Ratnadewi, Anak Agung
Indonesian Chimica Letters Vol. 2 No. 1 (2023)
Publisher : Department of Chemistry, Faculty of Mathematics and Natural Sciences, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/icl.v2i1.365

Abstract

Uricase enzyme (urate oxidase) is an enzyme that catalyze the oxidation of uric acid in the presence of oxygen to produce allantoin, carbon dioxide (CO2) and hydrogen peroxide (H2O2). Human and primates do not have this enzyme while other mammals have it in the liver therefore the uricase enzymes are extracted from goat liver use a buffer that is compatible with the human buffer system. The type of buffer selected adjusted at the appropriate pH. The optimum uricase pH ranged from 7.5 to 9.5. The selection of buffer type is adjusted to the human buffer system. The purpose of the study was to determine the effect of the type and pH of the extraction buffer on the total activity, protein total and specific activity of crude uricase. The types of buffer selected are phosphate buffer and carbonate buffer, while the selected pH is 7.5; 8.5; and 9.5. The method used is enzyme extraction, then determination of enzyme activity and protein content to determine the specific activity of the enzyme. The results obtained the highest total enzyme activity at pH 8.5 both in carbonate buffer (0.0481 U/mL) and phosphate buffer (0.0383 U/mL). The highest protein total in carbonate buffer was at pH 9.5 (4.55 mg/mL) while the highest value was in phosphate buffer pH 8.5 (4.1 mg/mL). The specific activity of uricase pH 8.5 was carbonate buffer (0.0114 U/mg) and phosphate buffer (0.0094 U/mg). The highest uricase specific activity value was at pH 8.5 for both carbonate and phosphate buffer types and in the long term it is used as a gout therapy.
Characterization of Polysulfone Membrane with Variation of Ethanol Concentrations in Coagulation Bath for Ultrafiltration Membrane Indarti, Dwi; Henry Adi Syahputra Sidabutar; Handayani, Wuryanti; Piluharto, Bambang
Indonesian Chimica Letters Vol. 2 No. 1 (2023)
Publisher : Department of Chemistry, Faculty of Mathematics and Natural Sciences, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/icl.v2i1.366

Abstract

The aim of this research is to study influence of ethanol concentrations in coagulation bath to charecteristic of physical properties and polysulfone membrane performance. Ethanol concentrations that used is 0; 40; 50; 60 ; 70; 80; 90 %. Process of making for polysulfone ultrafiltration membrane is prepared with phase inversion technique. Polysulfone membrane is made from polysulfone solved in N,N- dimetilacetamida (DMAc) and additive poly(ethylene glikol) (PEG) 600 in proportion 18 %: 77 %: 5 %. The result showed that increasing ethanol concentrations causes the increasing of density and increase concentrations from 0 - 50 % causes increasing swelling degree, but decreasing swelling degree from concentrations 60 - 90 %. However influence of ethanol concentration in coagulation bath to membrane performance is increasing of water flux, permeability coefficient, and rejection coefficient to dextran. It based on of result that polysulfone membrane with ethanol concentrations 80 dan 90 % included membrane classification ultrafiltration by rejection coefficient value above 90 %.
Co-Authors A. A. Istri Ratnawati AA. Istri Ratnadewi Achmad Sjaifullah Agung Budi Santoso Anak Agung Istri Ratnadewi Andika Ade Kurniawan, Andika Ade Andriana Kusuma Pertiwi Asnawati Asnawati Asnawati Aulia, Eldiani Bambang Piluharto Belkis, Malika Busroni Citra, Priscillia Denny Trias Utomo, Denny Trias Dwi Indarti Esti Utarti Eva Tyas Utami Febriani, Nurul Afifah Firdausa, Fita Kurnia Henry Adi Syahputra Sidabutar I Nyoman Adi Winata Ika Oktavianawati Istri Ratnadewi, Anak Agung Kristiyono, Rimba Candra Kumalasari, Ardine Kumalasari, Ardine Letisya, Nanda Leyla Novita Brigiyanti Linda Faiqotul Himmah Medayani, Rani Dian Muhamad Kiki Afindia Joenata Muhammad Reza Nasrul Amaliyatun Naja NG. Krishnabudi Ni Nyoman Tri Puspaningsih Novita Andarini Nugraha, Ari Satya Nyoman Gede Krishnabudi Oktavianawati , Ika Riki Juni Krismiadi Rohmawati, Manis Siti Nur Avida Sudarko Sudarko Sudarko Tri Agus Siswoyo Tri Mulyono Tri Mulyono Utari, Dwi Purwita Yeni Maulidah Muflihah Yudi Aris Sulistiyo, Yudi Aris