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All Journal PROSIDING SEMINAR NASIONAL Jurnal Biologi Tropis
Yusfi Afidah
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology

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Journal : Jurnal Biologi Tropis

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DNA Barcode Analysis of Dendrobium sp. Based On rbcL Sequence Dwinianti, Edia Fitri; Durotun Ainiyah; Yusfi Afidah; Mukhamad Su'udi
Jurnal Biologi Tropis Vol. 25 No. 4 (2025): Oktober-Desember
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i4.10177

Abstract

Morphological characterization of Dendrobium orchids needs to be supported by the utilization of DNA barcoding as a faster and more accurate approach. This study aims to identify the rbcL marker as a potential barcode for Dendrobium sp. collected from Jenggawah, Jember. The isolation of Dendrobium sp. genomic DNA was performed using the CTAB method, and the isolated DNA was amplified using PCR. The PCR products were used for sequencing analysis, and the sequence data were analyzed using several bioinformatics programs namely BLAST, ClustalX 2.1, and MEGA11. The results showed that the Dendrobium sp. sequence had a high level of homology with Calanthe alpina (MN192480.1), with a genetic similarity of 99.82%. The sequence analysis results of rbcL in Dendrobium sp. showed low genetic variation, which made rbcL less effective for use as a molecular marker at the species level. The rbcL barcode showed universal results for Dendrobium sp., so it needs to be supported by molecular identification using other markers such as matK and ITS for a more effective identification method in distinguishing between species.
Co-Authors Durotun Ainiyah Dwinianti, Edia Fitri Fatimatuz Zuhro Hasni Ummul Hasanah Hoesain, Mohammad Mukhamad Su'udi Sugeng Winarso