Article Per Year (5 Year)
p-Index From 2021 - 2026
0.61
P-Index
This Author published in this journals
All Journal Jurnal Sain Veteriner Jurnal Ilmu Ternak Veteriner WARTAZOA Indonesian Bulletin of Animal and Veterinary Sciences JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) Jurnal Medik Veteriner
Tatan Kostaman
Unknown Affiliation
Biochemistry, Genetics & Molecular Biology Veterinary

Published : 12 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 12 Documents
Search

 1   2 
Primordial Germ Cells Quality of Chicken Embryo from Post Cryopreservation Blood Samples Azizah, Nurul; Kostaman, Tatan; Tribudi, Yuli Arif; Kusumaningrum, Diana Andrianita; Suyatno, Suyatno; Muttaqin, Zultinur; Koswara, Eko; Gunawan, Muhammad
Jurnal Medik Veteriner Vol. 7 No. 2 (2024): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol7.iss2.2024.292-299

Abstract

The optimal cellular reservoir for the conservation and propagation of endangered poultry is acknowledged to be primordial germ cells (PGCs). This study aimed to evaluate and compare the efficacy of cryopreserving PGCs derived from purified sources and blood samples. The experimental design encompassed two treatment groups, each iterated six replication. Blood samples that had been filled with 500 µL of phosphate-buffered saline without Ca2+ and Mg2+ (PBS) underwent centrifugation at 1200 rpm for six minutes. The supernatant was discarded and the pellet was mixed with 500 µL 10% FBS-PBS and was then subdivided for cryopreservation and PGCs purification. Cryopreservation included the controlled freezing of blood and purified PGCs samples in Nalgene cryogenic vials with the incorporation of banker cells. Following thawing, statistical analyses revealed no significant variance in the mean PGCs count between purified PGCs and blood samples (p > 0.05). The percentages of PGCs mortality and the recovery rate demonstrated statistical significance (p < 0.05). Further investigations indicated that cryopreserving blood samples significantly increased the recovery rate of PGCs while decreasing post-thaw mortality. Morphology of PGCs from blood cryopreservation showed similar morphology to PGCs in general. The potential application of cell-based cryo-banking will contribute to the preservation of poultry genetic diversity for sustainability and adaptation to future poultry demands.
The Conservation on Indonesian Native Chicken Biodiversity Through Primordial Germ Cells Cryopreservation Tribudi, Yuli Arif; Kostaman, Tatan
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 9, No 1 (2022): JITRO, January
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (555.682 KB) | DOI: 10.33772/jitro.v9i1.20400

Abstract

ABSTRACT Primordial germ cells (PGCs) can be cryopreserved and used as a potential tool for the preservation of poultry biodiversity. By transplanting donor PGCs into recipient embryos to produce chimera germline, PGCs can migrate to developing gonads where germ cells are produced, thus enabling reproduction of offspring derived from donor PGCs via mating of chimera germline. Collection of PGCs from the circulation of chickens. Fertile eggs were incubated in a portable incubator for 2.5 days. Purification of circulating PGCs with nycodenz. Then the PGCs were frozen using the 5% DMSO cryoprotectant and to determine the success of cryopreservation, the donor PGCs were transplanted into the recipient embryos. The number of circulating PGCs was 50.58 ± 5.79 cells per embryo, the percentage of PGCs viability after thawing was 80.18 ± 1.25%. From the results of the PGCs transplant by looking at the migration of donor PGCs in recipient embryos with PKH-16 staining, it is clear. In conclusion, PGCs cryopreservation in this study can generally support the survival of good chickens and it is hoped that this PGCs conservation protocol can be used for other native chickens.Keywords: PGC, cryopreservation, chicken, conservation
Co-Authors A B Dharmayanthi A.R. Setioko Cece Sumantri Diana Andrianita Kusumaningrum, Diana Andrianita I Ketut Suatama I Khaerunnisa K Kinoshita Koswara, Eko M. A. Setiadi M. Fachrudin Muhammad Gunawan NURUL AZIZAH Rona Saumy Safitry S Darwati S Sopiyana S Sopiyana, S S. Sopiyana Suyatno Suyatno T Akiyama Tuti L. Yusuf Yuli Arif Tribudi Zultinur Muttaqin