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Plantlet Formation and Acclimatization of Sugarcane cv. PS 881 with Different Types and Concentration of Auxin Prasetyo, Rendie; Sugiyono, Sugiyono; Proklamasiningsih, Elly; Dewi, Prita Sari
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 3 (2020): December 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i3.23482

This research has been carried out with a view to induce rooting andÂ plantlet formation, followed by acclimatization.Â Micro shoots of sugarcane cv. PS 881 were cultured on Murashige and Skoog medium supplemented with different types and concentration of auxins for root induction. This research conducted experimentally using a split-plot design. The main plots were three types of auxins, which consisted of IAA, IBA, and NAA. The subplots were auxin concentrations with four levels, i.e. 0 Î¼M, 5 Î¼M, 10 Î¼M, and 15 Î¼M. Significantly faster root emergence time and higher number of roots observed in the Murashige and Skoog basal medium supplemented with 10 Î¼M NAA. The best root length obtained in the Murashige and Skoog basal medium supplemented with NAA 0 Î¼M. Plantlets derived from NAA 10 Î¼M treatment showed the best performance during acclimation with a 100% survival rate. NAA at a concentration of 10 ÂµM considered to be the best treatment inÂ plantlet formation and acclimatization of sugarcane cv. PS 881. This study showed that the use of MS medium with 10 uM NAA is able to increase the growth of PS 881 sugarcane plantlets. The results of this study can increase the availability of high quality seedlings and increase national sugar production.

Improving Agarwood (Aquilaria malaccensis Lamk.) Plantlet Formation Using Various Types and Concentrations of Auxins Tamyiz, Maulana; Prayoga, Lucky; Prasetyo, Rendie; Murchie, Erik Harry; Sugiyono, Sugiyono
Caraka Tani: Journal of Sustainable Agriculture Vol 37, No 1 (2022): April
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/carakatani.v37i1.58370

Aquilaria malaccensis Lamk. is one of the most widespread agarwood-producing plants that face extinction due to overexploitation. Agarwood propagation using in vitro culture techniques is capable of producing large quantities of plants in a shorter time and free from pests and diseases. Therefore, this study was conducted to analyze the effect of auxins type and concentration on agarwood plantlet formation using a split-plot design. The main plot was the type of auxin which included IAA, IBA and NAA, while the subplot was the concentration used which consisted of 0; 5; 10; 15 and 20 µM. The variable observed was agarwood plantlet formation with parameters measured including the number of shoots and leaves, plant height, and number of roots. The results showed that the formation of agarwood plantlets was controlled by the type, concentration, and interaction between the type and concentration of auxin. Furthermore, explants cultured on Murashige Skoog (MS) medium supplemented with 10 µM IBA produced the highest number of shoots (3.39 shoots explant-1) and leaves (7.25 leaves explants-1), while the addition of 10 uM NAA resulted in the highest number of roots (2.52 roots explant-1). This is the first time a study is conducted to evaluate the effect of type and concentration of auxins on agarwood plantlet formation. The production of high-quality shoots and plantlets increased agarwood germplasm availability to prevent extinction and support sustainable production.

PENGARUH ZAT PENGATUR TUMBUH TERHADAP MULTIPLIKASI TUNAS DAN BAHAN PENYANGGA PADA PEMBENTUKAN PLANTLET KANTONG SEMAR ADRIANII (Nepenthes adrianii) DENGAN KULTUR IN VITRO Egi Nuryadin; Sugiyono Sugiyono; Elly Proklamasiningsih
Bioeksperimen: Jurnal Penelitian Biologi Vol 3, No 2: September 2017
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v3i2.5180

Nepenthes adrianii (Kantong Semar) merupakan tanaman endemik khas Gunung Slamet, termasuk dalam Convention on International Trade of Endangered Species (CITES) terdapat apendiks I (Tahun 2003) dan II yaitu tanaman ini tergolong hampir punah dan langka. Mengingat besarnya potensi yang dimiliki tanaman ini, maka perlu adanya upaya konservasi untuk mengembangkan dan melestarikannya. Penerapan bioteknologi kultur jaringan atau kultur in vitro merupakan solusi yang tepat untuk melestarikan dan mengembangkan tanaman ini. Kultur in-vitro digunakan untuk multiplikasi tunas dan pembentukan plantlet.Penelitian ini bertujuan untuk: 1) mempelajari pengaruh interaksi BAP dan NAA pada multiplikasi tunas: 2) menentukan konsentrasi interaksi BAP dan NAA yang paling baik untuk memacu multiplikasi tunas; 3) mempelajari pengaruh bahan penyangga eksplan dan NAA pada pembentukan plantlet kantong semar (Nepenthes adrianii); dan 4) menentukan jenis bahan penyangga eksplan dan konsentrasi zat pengatur tumbuh NAA yang paling baik untuk memacu pembentukan plantlet kantong semar (Nepenthes adrianii).Metode yang digunakan adalah metode eksperimental, penelitian ini terdiri atas 2 tahap yaitu multiplikasi tunas dan pembentukan plantlet. Tujuan penelitian pada tahap multiplikasi tunas adalah untuk perbanyakan tunas dan tujuan penelitian pada tahap pembentukan plantlet adalah untuk mendapatkan tanaman kecil yang sejati. Tahap Multiplikasi Tunas menggunakan Rancangan Acak Lengkap (RAL) pola faktorial dengan 2 faktor. Faktor I adalah konsentrasi BAP yang terdiri dari 4 taraf yaitu 0 µM, 5 µM, 10 µM, dan 15 µM, Faktor II konsentrasi NAA yang terdiri dari 4 taraf yaitu yaitu 0 µM, 0,5 µM, 1 µM, dan 1,5 µM. Tahap Pembentukan Plantlet menggunakan Rancangan Petak Terpisah (Split Plot Design) sebagai petak utama adalah bahan penyangga eskplan (P) yaitu: agar dan jembatan kertas saring, sedangkan sebagai anak petak adalah konsentrasi NAA yang terdiri dari 4 taraf yaitu 0 µM, 5 µM, 10 µM, dan 15 µM. Parameter yang diukur dalam multiplikasi tunas yaitu waktu muncul tunas, waktu muncul daun, waktu muncul akar, jumlah tunas, jumlah daun dan jumlah akar. Parameter yang diukur dalam pembentukan plantlet yaitu jumlah tunas, jumlah daun, jumlah akar, daun terpanjang, akar terpanjang dan tinggi tanaman. Data yang diperoleh dianalisis menggunakan Analisis Ragam (Anova : Analysis of Variance) dengan tingkat kepercayaan 95%. Pengujian F menunjukan hasil sangat nyata kemudian dilanjutkan dengan uji Beda Nyata Jujur (BNJ) untuk mengetahui perbedaan antar perlakuan.Hasil penelitian menunjukan bahwa pada tahap multiplikasi tunas terbaik didapat pada perlakuan interaksi BAP 10 mM dan NAA 0,5 mM. Tahap Pembentukan plantlet terbaik di dapat pada perlakuan interaksi bahan penyangga jembatan kertas saring dengan zat pengatur tumbuh NAA 5 mM dan 10 mM.

UPAYA MEMACU PERTUMBUHAN TUNAS MIKRO KENTANG KULTIVAR GRANOLA DENGAN JENIS DAN KONSENTRASI SITOKININ BERBEDA Adinda RN Pratama; Sugiyono Sugiyono; Lucky Prayoga; Ali Husni
Scripta Biologica Vol 1, No 3 (2014)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Micro-shoot growth is the first step in the potato microtuber formation. The main factors were known controlling microtuber formation including the media type, the concentration of sucrose, the type and concentration of plant growth regulators, temperature, and photoperiodicity. This research aimed to determine the influence of the t of cytokinin and its concentration on the micro-shoot formation of Granola cultivar of potato (Solanum tuberosum L.), as well as to determine the best cytokinin type and its concentration on micro-shoot formation of Granola cultivar potato (Solanum tuberosum L.). The research was experimental in a split-plot design. The main plot was the type of cytokinin (BAP and Kinetin), and the subplot was cytokinin concentration at five levels of concentrations, i.e., 0, 5, 10, 15 and 20 µM. This study replicated each treatment three times which resulted in obtaining 30 experimental units. The data obtained were then analyzed using an analysis of variance, followed by honest significant difference test at 95 and 99% levels of confidence. The results showed that the formation of micro-shoot of Granola cultivar of potato was controlled by the type and concentration of cytokinin used. The addition of 5‒15 μM of BAP was found to be the best treatments to stimulate micro-shoot formation of Granola cultivar of potato.

The Application of Two Steps Culture in Agarwood, Aquilaria malaccensis, In Vitro Culture Improves Microshoots Induction and Development Vindi Ambara Putri; Sugiyono Sugiyono; Lucky Prayoga; Rendie Prasetyo; Sharon Hilary
Scripta Biologica Vol 9, No 1 (2022)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.sb.2022.9.1.1373

Agarwood (Aquilaria malaccensis) is an important species with high economic value and has many benefits, which led to an overexploitation in its natural habitat. An effort to both provides sufficient seedling for agarwood cultivation and conservation is therefore much needed. This study has been carried out with a view to determine the effect of media types and BAP concentrations in two steps cultivation system on agarwood microshoot induction. This was a two stage-experiments i.e., microshoots induction and optimizing shoot development. The research results showed that the interaction between different media type and BAP concentrations had no significant effect on agarwood microshoot induction. Subsequent culture on MS media without any BAP addition showed that explant derived from MS medium solidified with 2.5 gr. L-1 Phytagel produced 2.36 ± 0.48 shoots/explant and 3.69 ± 1.16 leaves/explant. In addition, explant derived from culture on MS medium supplemented with 4 µM BAP produced 2.28 ± 0.61 shoots/explant. This is for the first time the application of two steps culture system for Agarwood (Aquilaria malaccensis) has been deployed and how the habituation phenomenon is handled.

Banana Cultivars Microshoot Induction and Plantlet Formation Using Cytokinin and Auxin Sugiyono Sugiyono; Prita Sari Dewi; Rendie Prasetyo
Caraka Tani: Journal of Sustainable Agriculture Vol 36, No 2 (2021): October
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/carakatani.v36i2.50425

Banana is a horticultural plant with very high potentials, which contains carbohydrates and vitamins that are useful in fulfilling people's food and nutritional needs. Hence, this study aims to produce superior banana seedlings and develop a protocol for their mass production using a plant in vitro culture technique. This was a two stage-experiment i.e. microshoot production and plantlet formation. The result showed that Gebyar cultivar produced more shoots than the Kepok Kuning cultivar, with an average of 4.25 microshoots explant-1. However, Kepok Kuning produced more leaves than Gebyar, with an average of 4.64 leaves plantlet-1. Banana shoots cultured on the media containing Indole-3-acetic acid (IAA) at a concentration of 2.5 µM produced the highest leaves number. Meanwhile, those cultured on the media containing 1-Naphthalenesacetic acid (NAA) at a concentration of 7.5 µM produced the highest roots number. A Murashige and Skoog (MS) medium supplemented with 6-Benzylaminopurine (BAP) up to 30 µM and the one supplemented with 7.5 μM of NAA are suitable for Kepok Kuning and Gebyar cultivars micropropagation with regard to microshoot induction and plantlet formation, respectively.

The Effect of Media Types and NAA Concentrations on Agarwood (Aquilaria malaccensis Lamk.) Shoot Development in In Vitro Culture Ilham Warfa’ni; Lucky Prayoga; Rendie Prasetyo; Erik Harry Murchie; Sugiyono Sugiyono
AgriHealth: Journal of Agri-food, Nutrition and Public Health Vol 3, No 1 (2022): April
Publisher : Research and Development Center for Food, Nutrition and Public Health (P4GKM) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/agrihealth.v3i1.60346

Agarwood (Aquilaria malaccensis Lamk.) is a forest product commodity with high economic value, but its availability in nature is decreasing due to deforestation and overexploitation. The availability of high-quality seedlings is necessary to meet the demand and conserve the sources of agarwood plants. Therefore, this study investigates the interaction effect between media types and 1-Naphthaleneacetic acid (NAA) concentrations on agarwood shoot development. It also aims to determine the best medium type and NAA concentration to stimulate agarwood shoot development. This research had two stages, namely the shoot induction and development. The shoot induction stage was conducted experimentally, using a completely randomized design (CRD) on a factorial treatment pattern of 2 factors i.e., the types of media and NAA concentrations. The shoots produced from the induction stage were cultured on Murashige-Skoog (MS) media with the same media types but without any NAA addition. Furthermore, the variables observed in both stages were the development of agarwood shoots, and the parameters measured included the number of shoots, number of leaves and shoot lengths. The results showed that the development of agarwood shoots was controlled by the type of medium used. Liquid MS medium supported with filter paper bridge produced the highest average number of shoots with 4.93 and 5.87 shoots explants-1 and length at 3.25 and 3.64 cm explant-1 in the induction and development stage, respectively. These findings will facilitate mass propagation of agarwood shoots, and in turn the availability of agarwood plantlets supports its conservation and production.

The Improvement of Mini Tuber Production of Granola Potato Cultivar in Aeroponics System Sugiyono Sugiyono; Lucky Prayoga; Elly Proklamasiningsih; Khavid Faozi; Rendie Prasetyo
Biosaintifika: Journal of Biology & Biology Education Vol 13, No 1 (2021): April 2021
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v13i1.27714

Potato is an important food crop in both developed and developing countries and it is the fourth most important food crop after wheat, maize, and rice. Potato production in Indonesia has decreased in recent years, mainly due to the use of low quality tuber seed. The objective of this research was to determine the effect of plant materials, types of nutrients, and planting density on mini tuber formation, as well as to determine the best aeroponics conditions for mini tuber formation of Granola cultivar of potato. This research has been carried out experimentally using a Split-split Plot Design. The main plot was planting materials which consisted of plantlet and knol. The subplot was the type of nutrition media which consisted of Farran nutrition, Otazu nutrition, and AB-commercial mix. The sub-sub-plot was planting density which consisted of 50, 75, and 100 plants/m2. The research results showed that knol planted in aeroponics system on AB-commercial mix at a planting density of 75 plants/m2 resulted in the best mini-tuber production. In this study, for the first time three important factors for potato mini-tuber production have thoroughly been investigated in Indonesia. This finding will be used by Kledung Horticultural Seed Garden, Temanggung, for mass production of high-quality mini-tuber seed, which in turn will provide sufficient tuber seed for the farmer in its surrounding areas. By increasing the production of high-quality tuber seed, it is expected that it will contribute to the improvement of potato productivity and increase Indonesian potato production.

Plantlet Formation and Acclimatization of Sugarcane cv. PS 881 with Different Types and Concentration of Auxin Rendie Prasetyo; Sugiyono Sugiyono; Elly Proklamasiningsih; Prita Sari Dewi
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 3 (2020): December 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i3.23482

This research has been carried out with a view to induce rooting andÂ plantlet formation, followed by acclimatization.Â Micro shoots of sugarcane cv. PS 881 were cultured on Murashige and Skoog medium supplemented with different types and concentration of auxins for root induction. This research conducted experimentally using a split-plot design. The main plots were three types of auxins, which consisted of IAA, IBA, and NAA. The subplots were auxin concentrations with four levels, i.e. 0 Î¼M, 5 Î¼M, 10 Î¼M, and 15 Î¼M. Significantly faster root emergence time and higher number of roots observed in the Murashige and Skoog basal medium supplemented with 10 Î¼M NAA. The best root length obtained in the Murashige and Skoog basal medium supplemented with NAA 0 Î¼M. Plantlets derived from NAA 10 Î¼M treatment showed the best performance during acclimation with a 100% survival rate. NAA at a concentration of 10 ÂµM considered to be the best treatment inÂ plantlet formation and acclimatization of sugarcane cv. PS 881. This study showed that the use of MS medium with 10 uM NAA is able to increase the growth of PS 881 sugarcane plantlets. The results of this study can increase the availability of high quality seedlings and increase national sugar production.

Modification of Media Formulation and Agar Concentration to Improve Pitcher Plant (Nepenthes mirabilis (Lour.) Druce) Micropropagation for Conservation and Microfloriculture Development Andes Khofifah Putri; Rendie Prasetyo; Elly Proklamasiningsih; Paul A. Davison; Sugiyono Sugiyono
AgriHealth: Journal of Agri-food, Nutrition and Public Health Vol 5, No 1 (2024): April
Publisher : Research and Development Center for Food, Nutrition and Public Health (P4GKM) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/agrihealth.v5i1.85361

The pitcher plant (Nepenthes mirabilis (Lour.) Druce) is a unique plant listed in the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES) Appendix II and is protected in Indonesia. Conventional propagation of N. mirabilis is difficult and takes a longer time. Therefore, in vitro culture method is proposed. This study aimed to determine the best and most economical media formulation and agar concentration for N. mirabilis micropropagation. This research has been carried out experimentally using a completely randomized two-factor factorial design. The first factor was the media formulation (full-strength Murashige and Skoog (MS), half-strength MS, half-strength MS + AB mix, and AB mix) and the second factor was agar concentrations (6, 8, and 10 g l-1). Twelve treatment combinations were obtained and repeated 5 times to produce 60 experimental units. The explants were apical microshoots (1.5 cm long with 5 leaflets). The cultures were incubated at 24 ℃ under continuous light for 16 weeks. The parameters measured included shoot emergence time, number of shoots, number of leaves, and shoot length. The data were analyzed using variance analysis followed by Duncan’s multiple range test at a 95% confidence level. The results showed that half-strength MS medium resulted in the highest number of shoots and leaves and the longest shoot length, whereas adding 8 g l-1 agar resulted in the fastest shoot emergence time. Half-strength MS medium solidified with 8 g l-1 agar could produce many N. mirabilis (Lour.) Druce microshoots to support both conservation and microfloriculture development.

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