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PROFIL METABOLIT AKAR HANJELI (Coix lacryma-jobi l.) KETAN DAN HANJELI BATOK DI SUKABUMI Pratiwi, Zahra Apriyani; Nilawati, Tina Safaria; Kusdianti, Kusdianti
BIOTIKA Jurnal Ilmiah Biologi Vol 23, No 2 (2025): BIOTIKA DESEMBER 2025
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/biotika.v23i1.61537

Abstract

Penelitian ini bertujuan untuk mendapatkan profil metabolit sekunder yang terkandung dalam akar dan daun hanjeli kultivar ketan dan batok serta aktivitas antioksidan pada akar dan daun hanjeli batok. Sampel akar dan daun hanjeli diambil dari Desa Wisata Hanjeli, Kabupaten Sukabumi. Sampel diekstrak mengunakan metode maserasi dengan pelarut etanol p.a. 70%. Kandungan metabolit akar dan daun hanjeli dianalisis menggunakan Gas Chromatography-Mass Spectrometry (GC-MS) dan diidentifikasi menggunakan pustaka WILLEY09TH. Hasil penelitian menunjukkan akar hanjeli ketan mengandung 9 senyawa, sedangkan akar hanjeli batok mengandung 5 senyawa. Kedua akar hanjeli didominasi oleh senyawa 4-vinilfenol (Akar hanjeli ketan = 24.40%, akar hanjeli batok = 35.12%) yang termasuk ke dalam golongan fenolik. Pada akar hanjeli ketan dan hanjeli batok terdapat 4 senyawa golongan fenolik yang sama dengan konsentrasi yang berbeda yaitu 4-vinilfenol, 2-Metoksi-4-vinilfenol, koniferil alkohol, dan 4-allil-2,6-dimetoksifenol. Penelitian ini menunjukkan adanya perbedaan kandungan metabolit pada akar hanjeli dalam kultivar yang berbeda.
Response of Pinus merkusii Jung. & Devr. Megagametophyte Explants Cultured on DCR Medium with Different 2,4-D and Kinetin Combination Nabila, Afini Zulafa; Rahmat, Adi; Kusdianti, Kusdianti; Yulianty, Nurfitri
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Publisher : Universitas Pendidikan Indonesia and Konsorsium Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Pinus merkusii Jung. Devr. is a multipurpose tree species that is widely cultivated for timber, resin production, and land conservation. Nearly all parts of the tree are utilized, making it highly valuable. However, the high demand for its products is not met by its low natural regenerative capacity, which poses challenges for sustainable management. To address this, somatic embryogenesis, an in vitro propagation technique, has been explored as an alternative method for multiplying conifer species like Pinus merkusii. This study aims to assess the in vitro response of megagametophyte explants cultured on DCR medium with various combinations of 2,4-D and kinetin. The concentrations of 2,4-D tested were 0 µM, 7 µM, 9 µM, and 11 µM, while kinetin was tested at 0 µM, 2 µM, 3 µM, and 4 µM. The explants used were megagametophytes from female strobili measuring 5-7 cm, containing young zygotic embryos in the preembryo stage. After two months of cultivation in the dark, the explants exhibited responses such as enlargement, callus formation, and germination. The highest response rates for explant enlargement, callus formation, and germination were observed in the combinations of DK00 and DK04 (33.33%), DK70 and DK114 (33.33%), and DK72 and DK114 (44.44%). No somatic embryo formation was observed, possibly due to the age of the explants.