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All Journal Elkawnie ALCHEMY Jurnal Penelitian Kimia
Halimatussakdiah Halimatussakdiah
Samudra University
Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Energy Engineering Environmental Science Materials Science & Nanotechnology

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Anticancer Activity of N-Hexane Extract from Sphagneticola trilobata (L.) J.F Pruski Against MCF-7 Breast Cancer Cell Vivi Mardina; Tisna Harmawan; Halimatussakdiah Halimatussakdiah; Syafruddin Ilyas; Masitta Tanjung
Elkawnie: Journal of Islamic Science and Technology Vol 6, No 1 (2020)
Publisher : Universitas Islam Negeri Ar-Raniry Banda Aceh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/ekw.v6i1.6183

Abstract

Abstract : Sphagneticola trilobata (L.) J.F. Pruski is one of the perennial herbs that is widely used by the national and international community to treat various diseases including cancer. The objective of this study was to assessment the anticancer activity of n-hexane extract of S. trilobata leaves for inhibiting the growth of MCF-7 breast cancer cells in vitro by MTT (microculture tetrazolium salt) method. The n-hexane extract of sernai leaves was obtained from the maceration process of samples that were collected from the Langsa city, Aceh. The cytotoxicity test was carried out by incubating MCF-7 cells which had been exposed to several series of sample levels, viz. 1000; 500; 100; 50; 25; 10; 5 and 1 µg/mL. LC50 values are calculated using probit analysis. The results revealed that the n-hexane extract of S. trilobata leaves was cytotoxic against breast cancer cells (MCF-7) with an LC50 value of  0.037 μg /mL.Abstrak : Sphagneticola trilobata (L.) J.F. Pruski merupakan salah satu tanaman herbal yang digunakan secara luas oleh masyarakat nasional dan internasional untuk mengobati berbagai penyakit termasuk kanker. Penelitian ini bertujuan untuk mengetahui aktivitas antikanker ekstrak n-heksana daun S. trilobata dalam menghambat pertumbuhan sel kanker payudara MCF-7 secara in vitro dengan metode MTT (microculture tetrazolium salt). Ekstrak n-heksana daun sernai diperoleh dari proses maserasi sampel yang dikoleksi dari kota Langsa, Aceh. Uji sitotoksisitas dilakukan dengan menginkubasi sel MCF-7 yang telah dipaparkan beberapa seri  kadar sampel yaitu 1000, 500, 100, 50, 25, 10, 5 dan 1 µg/mL. Nilai LC50 dihitung dengan menggunakan analisa probit. Hasil penelitian menunjukkan bahwa ekstrak n-heksana daun S. trilobata bersifat sitotoksik terhadap sel kanker payudara (MCF-7) dengan harga LC50 sebesar 0,037 µg/mL. 
Free Radical Scavenging, Cytotoxic Activity, and Antioxidant-Targeted Molecular Docking Analysis of Akar Kusim Besar (Artabotrys sp.) Methanolic Extract from Ketambe, Indonesia Halimatussakdiah Halimatussakdiah; Ulil Amna; Vivi Mardina; Misdi Misdi
ALCHEMY Jurnal Penelitian Kimia Vol 22, No 1 (2026): March
Publisher : UNIVERSITAS SEBELAS MARET (UNS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/alchemy.22.1.108079.200-216

Abstract

Akar Kusim Besar (Artabotrys sp.), a vine from the Annonaceae family and an orangutan food source in Ketambe (Indonesia), is known to contain diverse bioactive metabolites. This study evaluated the phytochemical profile, antioxidant activity, cytotoxicity, and molecular docking of its methanolic leaf extract. Phytochemical screening confirmed the presence of alkaloids, steroids, terpenoids, saponins, flavonoids, phenolics, and tannins. The extract showed strong radical scavenging activity (IC50 = 100.816 µg/mL) and cytotoxicity against Artemia salina larvae (LC50 = 57.702 µg/mL), indicating potential bioactivity attributable to flavonoids and phenolic compounds. Gas Chromatography-Mass Spectrometry (GC-MS) analysis detected 30 compounds, of which 12 were subjected to molecular docking against human glutathione peroxidase 7 (2P31). Stigmasta-3,5-diene exhibited the strongest binding affinities, while several compounds complied with Lipinski's Rule of Five, supporting their potential druglikeness. Together, these findings suggest that Artabotrys sp. exhibits promising antioxidant and cytotoxic activities, and that molecular docking provides mechanistic insight into its anticancer potential.
Phytochemical Examination, GC-MS Analysis, and Antibacterial Activity of Methanol Extract of Dadap Leaves (Erythrina variegata L.) Against Staphylococcus aureus and Escherichia coli Bacteria using Disc Diffusion Method Yulia Theodora Situmorang; Halimatussakdiah Halimatussakdiah; Ulil Amna; Vivi Mardina
ALCHEMY Jurnal Penelitian Kimia Vol 22, No 1 (2026): March
Publisher : UNIVERSITAS SEBELAS MARET (UNS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/alchemy.22.1.99255.264-273

Abstract

This study examines the antibacterial activity of a methanol extract from the leaves of Dadap (Erythrina variegata L.) against Staphylococcus aureus and Escherichia coli. Bacterial infections are a major health issue in Indonesia, so the development of plant-based natural medicines is being pursued. The Dadap plant has the potential to treat bacterial infections. This study aims to identify the types of secondary metabolites in the methanol extract of Dadap leaves, analyze the active compounds using Gas Chromatography-Mass Spectroscopy (GC-MS), and evaluate their antibacterial activity. Extraction was performed by maceration with methanol, and antibacterial testing was conducted by disk diffusion on Mueller-Hinton Agar with extract concentrations of 5, 10, 20, and 40% (w/v). The extracts were diluted in sterile distilled water. The results exhibited antibacterial activity against S. aureus and E. coli, with inhibition zones of 14 mm (strong) and 8.8 mm (moderate) at a 40% concentration, respectively. These activities might be linked to the active compounds found in the Dadap leaves extract using phytochemical and GC-MS analysis. The phytochemical analysis showed that the E. variegata L. leaf extract contains alkaloids, steroids, terpenoids, saponins, flavonoids, phenols, and tannins. Furthermore, the GC-MS chromatogram identified 11 compounds, including 7 with antibacterial activity, such as neophytadiene, 3,7,11,15-tetramethyl-2-hexadecen-1-ol, hexadecanoic acid, methyl ester, n-hexadecanoic acid, phytol, methyl stearate, and stigmasterol. Overall, the crude extract of Dadap leaves showed a potential candidate for development as an antibiotic.
Anticancer Activity of N-Hexane Extract from Sphagneticola trilobata (L.) J.F Pruski Against MCF-7 Breast Cancer Cell Vivi Mardina; Tisna Harmawan; Halimatussakdiah Halimatussakdiah; Syafruddin Ilyas; Masitta Tanjung
Elkawnie Vol. 6 No. 1 (2020)
Publisher : Faculty of Science and Technology Universitas Islam Negeri Ar-Raniry

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/ekw.v6i1.6183

Abstract

Abstract : Sphagneticola trilobata (L.) J.F. Pruski is one of the perennial herbs that is widely used by the national and international community to treat various diseases including cancer. The objective of this study was to assessment the anticancer activity of n-hexane extract of S. trilobata leaves for inhibiting the growth of MCF-7 breast cancer cells in vitro by MTT (microculture tetrazolium salt) method. The n-hexane extract of sernai leaves was obtained from the maceration process of samples that were collected from the Langsa city, Aceh. The cytotoxicity test was carried out by incubating MCF-7 cells which had been exposed to several series of sample levels, viz. 1000; 500; 100; 50; 25; 10; 5 and 1 µg/mL. LC50 values are calculated using probit analysis. The results revealed that the n-hexane extract of S. trilobata leaves was cytotoxic against breast cancer cells (MCF-7) with an LC50 value of  0.037 μg /mL.Abstrak : Sphagneticola trilobata (L.) J.F. Pruski merupakan salah satu tanaman herbal yang digunakan secara luas oleh masyarakat nasional dan internasional untuk mengobati berbagai penyakit termasuk kanker. Penelitian ini bertujuan untuk mengetahui aktivitas antikanker ekstrak n-heksana daun S. trilobata dalam menghambat pertumbuhan sel kanker payudara MCF-7 secara in vitro dengan metode MTT (microculture tetrazolium salt). Ekstrak n-heksana daun sernai diperoleh dari proses maserasi sampel yang dikoleksi dari kota Langsa, Aceh. Uji sitotoksisitas dilakukan dengan menginkubasi sel MCF-7 yang telah dipaparkan beberapa seri  kadar sampel yaitu 1000, 500, 100, 50, 25, 10, 5 dan 1 µg/mL. Nilai LC50 dihitung dengan menggunakan analisa probit. Hasil penelitian menunjukkan bahwa ekstrak n-heksana daun S. trilobata bersifat sitotoksik terhadap sel kanker payudara (MCF-7) dengan harga LC50 sebesar 0,037 µg/mL. 
Co-Authors Masitta Tanjung Misdi Misdi Syafruddin Ilyas Tisna Harmawan Ulil Amna Vivi Mardina Yulia Theodora Situmorang