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All Journal HAYATI Journal of Biosciences Hemera Zoa Media Veteriner Forum Pasca Sarjana Jurnal Veteriner Ecotrophic, Journal of Environmental Science Microbiology Indonesia BERITA BIOLOGI Jurnal Matematika Sains dan Teknologi Indonesian Journal of Natural Pigments
BIBIANA W LAY
Department Of Biology, Faculty Of Biotechnology, Atma Jaya Catholic University, Jalan Jenderal Sudirman No. 51, Jakarta 12930, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Education Environmental Science Immunology & microbiology Mathematics Medicine & Pharmacology Veterinary Other

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Journal : Jurnal Veteriner

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POTENSI NETRALISASI IMUNOGLOBULIN Y ANTITETANUS YANG DIISOLASI DARI TELUR AYAM (THE POTENCY NETRALIZATION OF ANTI TETANUS IMMUNOGLOBULIN Y THAT WERE ISOLATED FROM CHICKEN EGGS) I Nyoman Suartha; I Wayan Teguh Wibawan; Retno Damayanti Soejoedono; Bibiana W. Lay
Jurnal Veteriner Vol 8 No 2 (2007)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The porpuse of study was to explore the potential use of? anti tetanus IgY from eggs yolk as a substitute for anti tetanus serum raised in ?horses. The eggs were collected from chickens which have previously been immunized with tetanus toxoid. Neutralization potency test of anti tetanus IgY determined by ?Spearman-Karber method.? The highest mean titer of anti tetanus of egg yolk was 80.16 ? 33.55 IU/ml and the lowest was 1.69 ? 0.63 IU/ml. The concentration? of purified IgY was 1.644 ? 0.424 mg/ml. Spearman-Karber value of potency of anti tetanus IgY are 35 IU/ml. ?This research concluded that Chickens was capable of produced of anti tetanus in eggs yolk with value of potency are 35 IU/ml.
Peranan Zona Pelusida Sebagai Barier Terhadap Cemaran Escherichia coli K99 (THE ROLE OF ZONA PELLUCIADA AS A BARRIER OF E COLI K99 CONTAMINATION) I Wayan Batan; Bibiana Widiati Lay; Ita Djuwita; Supar .; Arief Boediono
Jurnal Veteriner Vol 14 No 2 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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The aim of the study was to investigate the role of zona pelucida (ZP) as a barrier of embryo againstE.coli K99 contamination.   The complete randomized design was used in this study.  The embryos weregiven treatment as a follow : 1) embryos without ZP were contaminated with E.coli K99;  2) embryo withintact ZP were contaminated with E.coli K99;  and  3) embryos  with intact ZP were not contaminated withE.coli K99, as a control.  In each treatment there was 15 replication and in each replication there was oneembryo.  The embryos were incubated in incubator at 37°C and 5% CO2 atmosphere.  The embryos wereobserved every six hours in 24 hours using inverted microscope.  The result showed that embryos withintact ZP could develop in culture contaminated with  E.coli K99, while embryos without ZP becomedegenerated.  The viability of intact embryos was 75% and the embryos without ZP were 65%.  Embryosculture in contaminated medium could develop from eight cells embryo into morulla stage of embryo,compact morulla, and blastocyst. E.coli K99 contamination could inhibit embryo development.  In conclusion,ZP could protect embryo against E.coli K99 contamination.
Co-Authors A.A. Ketut Agung Cahyawan W Aisjiah Girinda Anas Miftah Fauzi ANASTASIA ASRI WIDYASARI Arief Boediono DWI ANDREAS SANTOSA Ekowati Handharyani Etty Riani Felianti, Felianti Hermonoadi Huminto Hernomoadi . Hernomoadi Hoeminto I Dewa Ketut Sastrawidana I Nyoman Suartha I Wayan Batan I wayan Teguh Wibawan Ita Djuwita KHUSNUL YAQIN latifah K Darusman Latifah K. Darusman M.B. Malole . Nurhasanah . Nurhasanah Nurhasanah Retno Damayanti Soejoedono Stella Magdalena Sugyo Hastowo Supar . Surjono Hadi Sutjahjo Warjoto, Renna Eliana Wiwin Winarsih