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All Journal JPSCR : Journal of Pharmaceutical Science and Clinical Research Jurnal Ilmiah Medicamento
Ni Putu Eka Leliqia
Jurusan Farmasi Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Udayana Bukit Jimbaran Badung, 80363, Indonesia
Environmental Science Health Professions Medicine & Pharmacology Public Health

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Jamur dari Mangrove sebagai Sumber Senyawa Metabolit Sekunder Bioaktif yang Berpotensi sebagai Kandidat Antikanker: Review Literatur Kristiani, Ni Kadek Ari; Putra, I Putu Yogi Astara; Putri, Ni Wayan Prasanthi Swarna; Leliqia, Ni Putu Eka; Ujam, Nonye Treasure; Ariantari, Ni Putu
Jurnal Ilmiah Medicamento Vol 9 No 2 (2023): Jurnal Ilmiah Medicamento
Publisher : Fakultas Farmasi Universitas Mahasaraswati Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36733/medicamento.v9i2.6910

Abstract

Cancer was the leading cause of death, which accounting for nearly 10 million deaths globally in 2020. Currently, cancer treatment still relies on chemotherapy, however, most anticancer drugs showed non-specific toxicity to normal cell proliferation resulting in various side effects, and are ineffective against many forms of cancer. In addition, the increasing case of chemoresistance of cancer cells to chemotherapy has boosted the discovery of new anticancer agents. Natural products are known as the origin of several clinically used anticancer agents, e.g. taxol and vincristine. Among natural products, mangrove-derived fungi are of particular scientific interest evidenced by the increasing rate of publications on cytotoxic secondary metabolites reported. Hence, this literature review aims to provide comprehensive information on cytotoxic secondary metabolites isolated from mangrove-derived fungi, which might contribute to the search for anticancer leads from natural resources. Data were collected from original research articles published on scientific-based sources such as Google Scholar, PubMed, Taylor and Francis, Elsevier, and MDPI, in the range of 2011-2022. Fifty-four cytotoxic secondary metabolites with IC50 values below 10 µM were described herein, which were classified in to 8 groups of metabolites. These compounds were reported from 16 genera of mangrove-associated fungi. Among them, Aspergillus and Penicillium were the most frequent producers of cytotoxic metabolites, suggesting their enormous potential as a source of pharmacophores for anticancer candidates.
Bioaktivitas dan Identifikasi Molekuler Jamur yang Diisolasi dari Sedimen di Pantai Amed dan Tulamben Wulandari, Ni Made Widya; Dwijayanti, Ni Kadek; Putri, Ni Putu Ananda Eka; Putra, I Putu Yogi Astara; Leliqia, Ni Putu Eka; Wibowo, Joko Tri; Dwija, Ida Bagus Nyoman Putra; Ariantari, Ni Putu
Jurnal Ilmiah Medicamento Vol 11 No 1 (2025): Jurnal Ilmiah Medicamento
Publisher : Fakultas Farmasi Universitas Mahasaraswati Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36733/medicamento.v11i1.10745

Abstract

Marine fungi have demonstrated the ability to produce promising new bioactive compounds. This study aims to isolate fungi from the marine sediments collected from Amed and Tulamben Beaches, Bali, Indonesia and investigate the bioactivity of the fungal extracts. Following fungal isolation, pure isolates were obtained and fermented on rice media containing salt and without salt. Next, secondary metabolites were extracted with ethyl acetate, then separated by liquid-liquid extraction using methanol containing 10% water and n-hexane. The resulting MeOH extracts were analyzed for their phytochemical content and tested for their antimicrobial and antioxidant activities using the disc diffusion and 2,2-diphenyl-1-picrylhydrazyl (DPPH) method, respectively. In total, three fungal isolates were obtained. Molecular identification from the Internal Transcribed Spacer (ITS) region of the isolates showed the closest relationship to Aspergillus sp., Aspergillus tamarii, and Trichoderma sp. Phytochemical analysis showed all fungal extracts contained alkaloids and polyphenols. Moreover, extract from Trichoderma sp. SED-TU (1) (NS) inhibited Staphylococcus aureus, Staphylococcus epidermidis, and Streptococcus mutans with inhibition zone diameters of 4.69±0.16; 3.51±0.01, and 3.49±0.25 mm, respectively. Extract from Aspergillus tamarii RM-S-SED-AM (S) inhibited Candida albicans with an inhibition zone of 7.04±0.22 mm and also had strong antioxidant activity with an IC50 value of 62.00±0.35 µg/mL. This study indicates that fungal isolates from marine sediments have great potential as a source of bioactive natural products.
Aktivitas Antimikroba dan Antioksidan Jamur Laut yang Berasosiasi dengan Spons Pseudoceratina sp. dari Perairan Pantai Amed Adi, I Made Agus Kusuma; Witantri, Ni Komang Diah Eka; Wulandari, Ni Made Widya; Leliqia, Ni Putu Eka; Wirajana, I Nengah; Wibowo, Joko Tri; Ware, Ismail; Ariantari, Ni Putu
Jurnal Ilmiah Medicamento Vol 12 No 1 (2026): Jurnal Ilmiah Medicamento (In progress)
Publisher : Fakultas Farmasi Universitas Mahasaraswati Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36733/medicamento.v12i1.13356

Abstract

Background: Marine fungi are organisms capable of growth and spore production in the marine environment and establishing symbiotic relationships with other marine organisms. Because of their high biochemical diversity, marine fungi are considered a potential source for the discovery of new natural compounds with various biological activities.Objective: This study aims to conduct molecular identification and assess the bioactivity potential of the methanol extract obtained from endophytic fungi associated with the marine sponge Pseudoceratina sp. from the Amed Coastal Waters, Bali.Methods: Eight marine fungi were isolated in this study and identified through molecular biology protocol as Aspergillus tamarii SP-3-1-4, Aspergillus nomiae SP-3-2 (A), Penicillium citrinum SP-3-2-1, Aspergillus protuberus SP-3-2-3, Aspergillus sydowii SP-3-2-4, Aspergillus sydowii RM NS SP-3B, Aspergillus clavatonanicus RM NS SP-3-2 A, and Aspergillus nomiae RM NS SP-3-2 B. The fungi were subsequently fermented on rice media containing salt and/or without salt. In the final stage of fermentation, secondary metabolites were extracted using ethyl acetate, followed by liquid-liquid extraction using methanol containing 10% water and n-hexane. The resulting methanolic extracts were subjected to phytochemical analysis and bioassays.Results: The phytochemical screening results of the methanol extracts showed that all extracts contained alkaloids. Among the tested extracts, the extract obtained from the fermentation of the fungus A. protuberus SP-3-2-3 on rice medium with the addition of salt showed the highest activity against Methicillin-resistant Staphylococcus aureus ATCC 3351 with an inhibition zone diameter of 7.99±0.20 mm. The fungus A. sydowii SP-3-2-4 cultured with and without salt in rice media exhibited the most potent antioxidant capacity, with IC50 values of 37.02±1.12 and 32.48±0.81 µg/mL. Conversely, A. nomiae SP-3-2 (A), fermented without salt, displayed the highest toxicity with an LC50 value of 0.88±0.69 µg/mL.Conclusion: Based on the pharmacological potential of extracts produced by the marine fungi associated with the sponge Pseudoceratina sp. found in the present study, identification of bioactive secondary metabolites and their mode of action is propitious for further investigation.
Potensi Krim Solid Lipid Nanoparticle (SLN) Kombinasi Ekstrak Bunga Gemitir dan Alga Merah untuk Mengatasi Jerawat akibat Propionibacterium acnes Suari, Anak Agung Istri Padma; Kartini, Ni Komang Triayu Cita; Bandem, I Gede Yudistira Perdangga; Hantari, Ni Kadek Dwi Cahyani Cipta; Leliqia, Ni Putu Eka; Ariantari, Ni Putu
JPSCR: Journal of Pharmaceutical Science and Clinical Research Vol 10, No 1 (2025)
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jpscr.v10i1.92332

Abstract

Bunga gemitir (Tagetes erecta L.) dan alga merah (Kappaphycus alvarezii (Doty) L.M.Liao) merupakan bahan alam yang telah dilaporkan memiliki aktivitas antibakteri dan antioksidan. Penelitian ini bertujuan untuk mengetahui efek antibakteri kombinasi ekstrak etanol bunga gemitir dan alga merah terhadap bakteri penyebab jerawat, Propionibacterium acnes ATCC 1223, serta aktivitasnya setelah diformulasi dalam bentuk sediaan sediaan krim solid lipid nanoparticle (SLN). Serbuk kering bunga gemitir dan alga merah diekstraksi dengan metode maserasi menggunakan pelarut etanol 96%. Terhadap ekstrak yang diperoleh, dilakukan skrining fitokimia. Selanjutnya, masing-masing ekstrak diuji aktivitas antibakterinya terhadap P. acnes secara individual dengan metode mikrodilusi. Aktivitas antibakteri kombinasi kedua ekstrak selanjutnya diuji dengan metode mikrodilusi checkerboard. Berdasarkan hasil pengujian aktivitas antibakteri, 4 perbandingan kombinasi ekstrak dibuat dalam bentuk sediaan krim SLN yaitu formula F1, F2, F3, dan F4. Sediaan F1–F4 dievaluasi melalui uji organoleptis, homogenitas, pH, daya sebar, dan daya lekat. Aktivitas antibakteri krim SLN diuji dengan metode difusi sumuran. Berdasarkan uji skrining fitokimia yang dilakukan, flavonoid, alkaloid dan fenol terdeteksi pada kedua ekstrak, sedangkan steroid hanya terdeteksi pada ekstrak bunga gemitir. Pada pengujian aktivitas antibakteri ekstrak secara individual, ekstrak bunga gemitir dan alga merah memiliki aktivitas lemah dengan nilai konsentrasi hambat minimum (KHM) sebesar 4 dan 16 mg/mL. Kombinasi ekstrak bunga gemitir dan alga merah menghasilkan aktivitas antibakteri lebih tinggi daripada aktivitas masing-masing ekstrak secara individual terhadap P. acnes, dan dikategorikan memiliki efek aditif dengan nilai FKI sebesar 0,5312. Setelah diformulasi dalam bentuk krim SLN, hanya formula F4 yang mengandung 0,1 g ekstrak bunga gemitir dan 0,2 g ekstrak alga merah memberikan aktivitas antibakteri terhadap P. acnes dengan diameter zona hambat sebesar 16,762,46 mm. Hasil penelitian ini menunjukkan sediaan krim SLN F4 yang mengandung kombinasi ekstrak bunga gemitir dan alga merah potensial untuk diinvestigasi lebih lanjut untuk pengembangan sediaan untuk mengatasi jerawat berbasis bahan alam. 
Co-Authors Adi, I Made Agus Kusuma Bandem, I Gede Yudistira Perdangga Dwija, Ida Bagus Nyoman Putra Hantari, Ni Kadek Dwi Cahyani Cipta I Nengah Wirajana Joko Tri Wibowo, Joko Tri Kartini, Ni Komang Triayu Cita Kristiani, Ni Kadek Ari Melinda, Meylana Ayu Ni Kadek Dwijayanti Ni Putu Ariantari Putra, I Putu Yogi Astara Putri, Ni Putu Ananda Eka Putri, Ni Wayan Prasanthi Swarna Sinta Devi, Ni Nyoman Ayu Sri Suari, Anak Agung Istri Padma Ujam, Nonye Treasure Ware, Ismail Wiryani, Ida Ayu Witantri, Ni Komang Diah Eka Wulandari, Ni Made Widya