Article Per Year (5 Year)
p-Index From 2020 - 2025
0.983
P-Index
This Author published in this journals
All Journal The Indonesian Biomedical Journal Poltekita : Jurnal Ilmu Kesehatan Abdimas: Jurnal Pengabdian Masyarakat Universitas Merdeka Malang Jurnal Ilmiah Medicamento Journal of Clinical Microbiology and Infectious Diseases
Dwija, Ida Bagus Nyoman Putra
Unknown Affiliation
Humanities Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health

Published : 5 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 5 Documents
Search

 1 
Various types of extended spectrum β-lactamases: a literature review Rastuti, Made Rina; Budayanti, Ni Nyoman Sri; Dwija, Ida Bagus Nyoman Putra
Journal of Clinical Microbiology and Infectious Diseases Vol. 3 No. 2 (2023): Available online: December 2023
Publisher : Indonesian Society for Clinical Microbiology (Perhimpunan Dokter Spesialis Mikrobiologi Klinik Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51559/jcmid.v3i2.52

Abstract

The inappropriate use of antibacterials causes the spread of resistance in bacteria and increases the health burden of infection due to the nature of resistance to many classes of antibiotics which are referred to as multi-resistant (multidrug resistant). Extended spectrum β-lactamase (ESBL) is one of the enzymes that cause multi-resistance, where bacteria with this enzyme become resistant to third-generation cephalosporin antibiotics which are often used to treat gram-positive and gram-negative bacterial infections in humans with minimal side effects. β -lactamase is an enzyme capable of hydrolyzing the β-lactam ring in β -lactam class antibiotics so that antibiotics become inactive. Mutations in the gene that encodes this enzyme produce a β-lactamase enzyme which can break down the β-lactam ring in all penicillin and cephalosporin antibiotics. The classification of β-lactamases is complex due to genetic, biochemical properties, and substrate affinity for β-lactamase inhibitors. There are also various types of ESBL enzyme-coding genes including TEM, SHV, CTX-M, VEB, PER, OXA, SFO-1, BEL-1, BES-1, TLA-1, and GES. This study aims to review various types of extended-spectrum β-lactamases.
Training hotel staff in early emergency management to enhance tourist comfort at Ubud Raya Resort area Ani, Luh Seri; Hendrayana, Made Agus; Gandari, Ni Komang Matalia; Laksmi, Ida Ayu Agung; Agastiya, I Made Cahyadi; Vittala, Govinda; Juniartha, I Gusti Ngurah; Dwija, Ida Bagus Nyoman Putra
Abdimas: Jurnal Pengabdian Masyarakat Universitas Merdeka Malang Vol. 9 No. 1 (2024): February 2024
Publisher : University of Merdeka Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26905/abdimas.v9i1.12128

Abstract

Tourists who stay at the hotel have a risk of illness and/or experiencing emergency problems that interfere with the sense of security and comfort for tourists. One factor for successful emergency management is first aid proficiency. However, the knowledge and skills of hotel staff are relatively lacking in handling emergency problems for tourists.  Based on this, this service was held to train the skills of hotel staff in handling emergencies for tourists. Service activities are carried out by presentation methods and practice on basic life support. The target of this activity is the hotel staff of Ubud Raya Resort. Dedication materials were given in 5 meetings. The training material has been adjusted to travel medicine subjects and based on the results of previous research by the implementation team. The implementation of this service went smoothly and as planned. Based on the results of the pretest and posttest, an increase in the knowledge and skills of participants was obtained. Based on interviews, it was found that service participants received positive benefits from the service carried out. Thus, concluded that this service was successfully carried out despite the limitations in its implementation. This community service advice re-conducting basic life support training for hotel staff so that the knowledge and skills received are beneficial in the long term.
Bioaktivitas dan Identifikasi Molekuler Jamur yang Diisolasi dari Sedimen di Pantai Amed dan Tulamben Wulandari, Ni Made Widya; Dwijayanti, Ni Kadek; Putri, Ni Putu Ananda Eka; Putra, I Putu Yogi Astara; Leliqia, Ni Putu Eka; Wibowo, Joko Tri; Dwija, Ida Bagus Nyoman Putra; Ariantari, Ni Putu
Jurnal Ilmiah Medicamento Vol 11 No 1 (2025): Jurnal Ilmiah Medicamento
Publisher : Fakultas Farmasi Universitas Mahasaraswati Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36733/medicamento.v11i1.10745

Abstract

Marine fungi have demonstrated the ability to produce promising new bioactive compounds. This study aims to isolate fungi from the marine sediments collected from Amed and Tulamben Beaches, Bali, Indonesia and investigate the bioactivity of the fungal extracts. Following fungal isolation, pure isolates were obtained and fermented on rice media containing salt and without salt. Next, secondary metabolites were extracted with ethyl acetate, then separated by liquid-liquid extraction using methanol containing 10% water and n-hexane. The resulting MeOH extracts were analyzed for their phytochemical content and tested for their antimicrobial and antioxidant activities using the disc diffusion and 2,2-diphenyl-1-picrylhydrazyl (DPPH) method, respectively. In total, three fungal isolates were obtained. Molecular identification from the Internal Transcribed Spacer (ITS) region of the isolates showed the closest relationship to Aspergillus sp., Aspergillus tamarii, and Trichoderma sp. Phytochemical analysis showed all fungal extracts contained alkaloids and polyphenols. Moreover, extract from Trichoderma sp. SED-TU (1) (NS) inhibited Staphylococcus aureus, Staphylococcus epidermidis, and Streptococcus mutans with inhibition zone diameters of 4.69±0.16; 3.51±0.01, and 3.49±0.25 mm, respectively. Extract from Aspergillus tamarii RM-S-SED-AM (S) inhibited Candida albicans with an inhibition zone of 7.04±0.22 mm and also had strong antioxidant activity with an IC50 value of 62.00±0.35 µg/mL. This study indicates that fungal isolates from marine sediments have great potential as a source of bioactive natural products.
The Overview of Nutritional Status and Blood Glucose Levels of Pregnant Women at the Ubud Health Center, Bali Rinawati, Luh Putu; Sarihati, I Gusti Agung Dewi; Sundari, Cok Dewi Widhya Hana; Dhyanaputri, I Gusti Ayu Sri; Bekti, Heri Setiyo; Dwija, Ida Bagus Nyoman Putra
Poltekita : Jurnal Ilmu Kesehatan Vol. 17 No. 4 (2024): February
Publisher : Poltekkes Kemenkes Palu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33860/jik.v17i4.2045

Abstract

  The nutritional status of pregnant women can be determined by measuring the size of mid-upper arm circumference (MUAC) and body mass index (BMI). Women with excessive nutritional status have a high risk of various complications. This study aims to describe the nutritional status and blood glucose levels of pregnant women at the Ubud Health Center, Bali. This descriptive study was conducted on 27 pregnant women who had antenatal care at the Ubud Health Center. MUAC size was measured using a measuring tape, while BMI was measured by calculating body weight in kilograms divided by height in meters squared. Blood glucose levels were measured using the point of care testing (POCT) method. From 27 respondents, it was found that 23 respondents had normal MUAC, 3 respondents had less than normal, and 1 respondent was obese. Based on BMI, 14 respondents had normal BMI, 5 respondents were overweight, and 8 respondents were obese. For blood glucose levels, all respondents have normal levels. Respondents who have MUAC less than normal can be at risk of chronic energy deficiency, while respondents with the obese category can be at risk of GDM, hypertension, and cesarean section. Pregnant women with GDM can increase the risk of type 2 DM, cardiovascular disease and have the potential to give birth macrosomic babies. Pregnant women with GDM almost never give complaints, so screening is necessary. Screening can be done by measuring the nutritional status of pregnant women and blood glucose levels during antenatal care at health center.
HPV18 E6/E7 Mutation and Their Association with The Expression Level of Tumor Suppressor Proteins p53 and pRb among Indonesian Women with Cervical Cancer Mahendra, I Nyoman Bayu; Prayudi, Pande Kadek Aditya; Dwija, Ida Bagus Nyoman Putra
The Indonesian Biomedical Journal Vol 17, No 1 (2025)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v17i1.3400

Abstract

BACKGROUND: The E6/E7 mutation contributes to the intra-typic variant of HPV18 which may differ in their oncogenic potential. E6 and E7 target the tumour suppressor protein p53 and pRb, respectively, and their degradation play a crucial role in cervical carcinogenesis. However, the prevalence of HPV18 E6/E7 variants among Indonesian women with cervical cancer has not been elucidated. Therefore, this study was conducted to characterize the HPV18 variants among Indonesian women with cervical cancer and their association with tumor suppressor protein p53 and pRb.METHODS: A hundred Indonesian women with pathologically proven cervical cancer were consecutively recruited into the study. Polymerase chain reaction (PCR) was performed to detect HPV18 DNA E6 and E7 oncogenes using specific primers and the variants was determined through nucleotide sequencing. Expressions of p53 and pRb were analyzed through immunohistochemistry by using specific antibodies targeting p53 and pRB.RESULTS: The rate of HPV18 positivity was 24%. The rate of E6 and E7 mutation was 45.4% and 59.1%, respectively. Those with E6 mutation had significantly higher expression of p53 and pRb as compared to those with wildtype E6 (p<0.05). Subjects with E7 mutation only had higher expression of pRb (p<0.05). Phylogenetic analysis revealed that 54.5% subjects had genetic sequences closely related to Asian lineages, particularly A1, A4, and A5 sublineage. Interestingly, 3 subjects had genetic sequences closely related to MK813921, a newly identified sequences. However, 45.5% subjects had distinct genetic sequences that did not related to the reference sequence used in this study.CONCLUSION: E6 and E7 mutation was common among Indonesian women with HPV18 cervical cancer and associated with the level of tissue p53 and pRb expression.KEYWORDS: HPV18 E6/E7, mutation, epidemiology, Indonesian women
Co-Authors Agastiya, I Made Cahyadi Cokorda Dewi Widhya Hana Sundari Gandari, NK. Matalia Heri Setiyo Bekti I Gusti Agung Dewi Sarihati I Gusti Ayu Sri Dhyanaputri I Gusti Ngurah Juniartha Joko Tri Wibowo, Joko Tri Laksmi, Ida Ayu Agung Luh Seri Ani Made Agus Hendrayana Ni Kadek Dwijayanti Ni Nyoman Sri Budayanti Ni Putu Ariantari Ni Putu Eka Leliqia Nyoman Bayu Mahendra Prayudi, Pande Kadek Aditya Putra, I Putu Yogi Astara Putri, Ni Putu Ananda Eka Rastuti, Made Rina Rinawati, Luh Putu Vittala, Govinda Wulandari, Ni Made Widya