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Uji Silang Digital sebagai Alternatif Uji Banding Manual Pemeriksaan Mikroskopis Malaria di Kulon Progo Elsa Herdiana Murhandarwati; Anis Fuad; Guntur Budi Herwanto; Rizqiani Amalia Kusumasari; Mahardika Agus Wijayanti; supargiyono supargiyono
Jurnal Pengabdian kepada Masyarakat (Indonesian Journal of Community Engagement) Vol 4, No 1 (2018): September
Publisher : Direktorat Pengabdian kepada Masyarakat Universitas Gadjah Mada

Malaria is an infectious disease in tropical countries including Indonesia which causes serious clinical manifestations and even death. Indonesia Ministry of Health targets that malaria is eliminated from this country by 2030. In Java, malaria is targeted to be eliminated by 2015. However, elimination has not been achieved because some areas, including Kulon Progo, Daerah Istimewa Yogyakarta often experiences outbreaks (Kejadian Luar Biasa) in recent years. As microscopic diagnosis is a gold standard diagnosis for malaria, thus accurate microscopic examination is important. Disappearance of malaria cases in malaria pre-elimination areas causes laboratory workers/microscopists are less exposed to experience in identifying malaria. As part of quality assurance, continuous quality control is expected to maintain the accuracy of malaria diagnosis in endemic areas. However, the cross-check sistem as a part of this quality control strategy still has constraints, such as: transportation costs, limited human resources and irregularity. Ibm team of Faculty of Medicine UGM has successfully developed a digital microscopic cross-check sistem. This method is able to bridge the distance and time constraint also transportation costs of slide submissions to be cross-checked to the referral laboratory. Our study partners were microscopists of Kulon Progo Primary Health Centers (Puskesmas) facilitated with digital devices installed in a microscope. Partners were trained to: 1. take microscopic images of suspected malaria parasites and 2. familiarize with digital cross-check sistem. The successful implementation of digital malaria cross-check is expected to improve the accuracy of malaria diagnosis and assist the Government in malaria elimination program especially in Kulon Progo. This system has been registered for Intellectual Property Right no. 091306 obtained from Department of Justice and Human Right of Republic of Indonesia.

Uji Validitas dan Efikasi Crypto/Giardia Duo-Strip dalam Mendeteksi Cryptosporidium spp. di Desa Tulehu, Kabupaten Maluku Tengah, Provinsi Maluku Rizqiani Amalia Kusumasari; Muh. Syairaji
Jurnal Kesehatan Vokasional Vol 5, No 3 (2020): Agustus
Publisher : Sekolah Vokasi Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jkesvo.55361

Latar Belakang: Cryptosporidium spp. adalah salah satu enteropatogen pada manusia dan umumnya ditemui terutama pada anak-anak di negara berkembang. Prevalensi Cryptosporidium spp. di Indonesia masih belum diketahui dan penelitian terkait juga masih sangat jarang. Saat ini, beberapa perusahaan telah mengembangkan tes diagnostik sederhana dan cepat untuk mendeteksi infeksi Cryptosporidium spp. tetapi informasi mengenai keakuratannya pada kondisi di Indonesia masih belum diketahui. Tujuan: Mengetahui validitas alat Crypto/Giardia Duo-Strip dan prevalensi Cryptosporidium spp. pada salah satu wilayah pedesaan di timur Indonesia.Metode: Penelitian ini menggunakan desain potong-lintang dan dilakukan tahun 2015. Sampel tinja dikumpulkan dari seluruh masyarakat di desa Hurnala, Maluku Tengah. Validitas (Sensitifitas, Spesifisitas) dan Efikasi (Nilai Prediktif Positif dan Negatif) dari Crypto/Giardia Duo-Strip dinilai menggunakan Modified acid-fast staining sebagai standar referensi. Hasil: Dari 731 sampel tinja, terdapat 261 sampel positif Cryptosporidium spp. menggunakan Modified acid-fast staining dan 204 sampel positif menggunakan Crypto/Giardia Duo-Strip. Sensitivitas, Spesifisitas, PPV, dan NPV Crypto/Giardia Duo-Strip masing-masing sebesar 38.78%, 78.99%, 35.77%, 69.64% dengan Likelihood Ratio+ (LR+): 1.77 and (LR-): 0.79. Kesimpulan: Prevalensi infeksi Cryptosporidium spp. di desa Hurnala, Maluku Tengah cukup tinggi yaitu 36% (261/731). Kemampuan alat Crypto/Giardia Duo-Strip masih relatif rendah untuk mendeteksi Cryptosporidium spp. di wilayah Indonesia.

Pelatihan mikroskopis dan entomologi pada tenaga laboratorium dan entomologis lokal di Kecamatan Kokap untuk mendukung eliminasi malaria di Kabupaten Kulon Progo, DIY Elsa Herdiana Murhandarwati; Rizqiani Amalia Kusumasari; Purwono Purwono; Kuswati Kuswati; Sulistyawati Sulistyawati; Sitti Rahmah Umniyati; Mahardika Agus Wijayanti; Tri Baskoro Tunggul Satoto
Journal of Community Empowerment for Health Vol 2, No 1 (2019)
Publisher : Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada

Malaria is a serious public health problem in Indonesia. This disease is one of several indicators that have been targeted by the Millennium Development Goals (MDGs) 2015 to become Sustainable Development Goals (SDGs). In malaria-endemic areas such as in Kokap, Kulon Progo, Yogyakarta, reported malaria cases declined in 2000 with no case, but increased in 2011 following by some outbreaks in 2012. Future outbreaks could be avoided when health personnel including Primary Health Care (PHC) microscopists and entomologist surveyors were equipped with adequate trainings related to malaria. This training also be used for refreshment for existing personnel to support elimination at the Kokap I, Kokap II, and several nearby PHCs. In October 2013, the Department of Parasitology, Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada carried out microscopic training and basic entomology related to malaria in the working area of PHC in Kokap. Training methods were giving material and direct activities in the field. The training provided basic entomology training, malaria microscopic training and malaria vector mapping using a Global Positioning System (GPS). The training facilitated PHC personnel to do correct identification of Plasmodium species, identification of old and new vector breeding sites, and found out how to map vector distribution and malaria cases in their area. The results showed that there was an increase in knowledge from PHC personnel who participated in the pre-test and post-test. From field activity, three adult mosquitoes species were found i.e. Anopheles vagus, An. annularis and An. maculatus. Nulliparous and parous mosquitoes from adult mosquito based on the examination of their tracheole skeins of ovarium were found from the collected mosquitoes although oocyst in midgut and sporozoite in salivary gland were not found during adult mosquito surgical. Similar trainings were expected to be applied in other similar areas in the future.

STUDENTS’ PERCEPTION ON THE USE OF A MASSIVE OPEN ONLINE COURSE FOR PARASITOLOGY: A PRELIMINARY STUDY Utomo, Prattama Santoso; Kusumasari, Rizqiani Amalia; Murhandarwati, Elsa Herdiana; Widyandana, Doni
Jurnal Pendidikan Kedokteran Indonesia: The Indonesian Journal of Medical Education Vol 12, No 2 (2023): Juni
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpki.82183

Background: The COVID-19 pandemic had a significant impact on medical education globally. The learning process has been shifted to online learning to ensure health and safety measures. Online learning approaches and applications are also established rapidly, including the Massive Open Online Course (MOOC), which can support learning in many topics and facilitate a large number of students effectively and efficiently. Department of Parasitology and Department of Medical Education and Bioethics, Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada developed the Central Nervous System (CNS) Parasitic Infection module as an online learning for both undergraduate and postgraduate levels.Aims: The study aimed to evaluate and explore students’ perception of the CNS Parasitic Infection online module development in an MOOC format.Methods: This is an evaluation study using an explorative qualitative approach. The pilot implementation of the CNS Parasitic Infection module was evaluated using in-depth interviews with ten (10) students of the Master in Tropical Medicine and the Master in Biomedical Science programs. The participants were requested to share their learning experience on the module, and their suggestions for module improvement. The interviews were conducted online using Zoom. The interview transcripts were analyzed using thematic analysis.Results: Implementing the CNS Parasitic Infection module using MOOC asynchronous approach received both positive and negative responses from participants. A total of 6 themes were identified from participants, for instance, learning outcomes achievement, interactive e-case design, learning flexibility, course timing, network issues, and the need for learning directions.Conclusion: CNS Parasitic Infection can be delivered to students using an asynchronous MOOC format. Students’ outcomes achievement was sufficient using this method, and overall, students appreciated the implementation of the program.

Uji Validitas dan Efikasi Crypto/Giardia Duo-Strip dalam Mendeteksi Cryptosporidium spp. di Desa Tulehu, Kabupaten Maluku Tengah, Provinsi Maluku Kusumasari, Rizqiani Amalia; Syairaji, Muh.
Jurnal Kesehatan Vokasional Vol 5, No 3 (2020): August
Publisher : Sekolah Vokasi Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jkesvo.55361

Latar Belakang: Cryptosporidium spp. adalah salah satu enteropatogen pada manusia dan umumnya ditemui terutama pada anak-anak di negara berkembang. Prevalensi Cryptosporidium spp. di Indonesia masih belum diketahui dan penelitian terkait juga masih sangat jarang. Saat ini, beberapa perusahaan telah mengembangkan tes diagnostik sederhana dan cepat untuk mendeteksi infeksi Cryptosporidium spp. tetapi informasi mengenai keakuratannya pada kondisi di Indonesia masih belum diketahui. Tujuan: Mengetahui validitas alat Crypto/Giardia Duo-Strip dan prevalensi Cryptosporidium spp. pada salah satu wilayah pedesaan di timur Indonesia.Metode: Penelitian ini menggunakan desain potong-lintang dan dilakukan tahun 2015. Sampel tinja dikumpulkan dari seluruh masyarakat di desa Hurnala, Maluku Tengah. Validitas (Sensitifitas, Spesifisitas) dan Efikasi (Nilai Prediktif Positif dan Negatif) dari Crypto/Giardia Duo-Strip dinilai menggunakan Modified acid-fast staining sebagai standar referensi. Hasil: Dari 731 sampel tinja, terdapat 261 sampel positif Cryptosporidium spp. menggunakan Modified acid-fast staining dan 204 sampel positif menggunakan Crypto/Giardia Duo-Strip. Sensitivitas, Spesifisitas, PPV, dan NPV Crypto/Giardia Duo-Strip masing-masing sebesar 38.78%, 78.99%, 35.77%, 69.64% dengan Likelihood Ratio+ (LR+): 1.77 and (LR-): 0.79. Kesimpulan: Prevalensi infeksi Cryptosporidium spp. di desa Hurnala, Maluku Tengah cukup tinggi yaitu 36% (261/731). Kemampuan alat Crypto/Giardia Duo-Strip masih relatif rendah untuk mendeteksi Cryptosporidium spp. di wilayah Indonesia.

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