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Respon pertumbuhan dan produksi padi beras merah (Oryza nivara) terhadap cekaman kekeringan pada fase pertumbuhan berbeda dan pemupukan nanosilika Rita Sugiarto; Budi Adi Kristanto; Dwi Retno Lukiwati
Journal of Agro Complex Vol 2, No 2 (2018)
Publisher : Department of Agriculture, Animal and Agricultural Sciences, Diponegoro University, Semara

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (573.879 KB) | DOI: 10.14710/joac.2.2.169-179

Abstract

The purpose of this research was to analyze the response of growth and production of red rice (Oryza nivara) under drought and nanosilica fertilization condition. This research was conducted in Greenhouse and Laboratory of and Plant Physiology and Breeding of at Faculty of Animal and Agricultural Sciences, Diponegoro University, Semarang from April to July 2017. The research was arranged using completely randomized factorial design. The first factor was drought stress (control, drought 20-35 DAP (Days After Planting), 40-55 DAP and 55-70 DAP). The second factor was the use of nanosilika fertilizers (without nanosilika, with nanosilica). The data were analyzed by anova and followed by Duncan’s Multiple Range Test (DMRT). The results showed that the drought stress during tiller production stage decreased the total number of tillers, productive tillers and grain weight per clump, but not in weight of one thousand seeds. Drought stress during flowering stage and seed filling only decreases the weight of one thousand seeds. Application of nanosilica increases the number of productive tillers and the weight of grain per hill. There was no interaction between drought stress treatment and nanosilika fertilization. Keywords: red rice, production, drought, nanosilica.
Respon pertumbuhan dan hasil cabai rawit (Capsicum frustescens l.) akibat inokulasi cendawan mikoriza arbuskular dan pemupukan fosfat Amalia Ramadhina Ghaisani; Dwi Retno Lukiwati; I Mansur
Journal of Agro Complex Vol 4, No 1 (2020): JOAC Online
Publisher : Department of Agriculture, Animal and Agricultural Sciences, Diponegoro University, Semara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/joac.4.1.50-59

Abstract

ABSTRACT This research aimed to identify the impact of various species of arbuscular mycorrhiza inoculation and various phosphate fertilizer and their interaction on growth and yield of chili (Capsicum frustescens L.). The research used factorial experiment 4x4 with complete randomized design and consisted of 3 replication. Mycorrhiza factor consisted of M0: without mycorrhiza, M1: Glomus agregatum 20 g/pot, M2: Acaulospora sp 20 g/pot dan M3: Glomus agregatum 10 g/pot ­+ Acaulosporasp 10 g/pot. Phosphate fertilizer factor consisted of P0: without phosphate fertilizer, P1: TSP 125 kg P2O5 /ha, P2: rock phosphate (RP) 125 kg P2O5/ha dan P3: guano 125 kg P2O5/ha. The research variables measured were plant height, number of leaves, number of branches, yield of chili and dry shoot weight. Data are processed by Analysis of Variance and continued by Duncan’s Multiple Range Test (DMRT). The result showed that G.agregatum + Acaulospora sp inoculation can increase number of leaves, meanwhile G.agregatum + Acaulospora sp, G.agregatum and Acaulospora sp inoculation can increase number of branches and yield of chili. TSP fertilizer can increase number of branches, meanwhile TSP, RP and guano fertilizer can increase plant height, number of leaves, yield of chili, dry shoot weight.Keywords : Capsicum frustescens, arbuscular mycorrhiza, TSP, rock phosphate, guano ABSTRAK Penelitian bertujuan untuk mengetahui pengaruh spesies CMA dan jenis pupuk fosfat yang berbeda, serta interaksinya terhadap pertumbuhan dan produksi cabai rawit. Penelitian menggunakan percobaan faktorial 4x4 dengan Rancangan Acak Lengkap (RAL) yang terdiri dari 3 ulangan. Faktor pertama adalah spesies inokulum CMA, yaitu M0: tanpa mikoriza, M1: Glomus agregatum 20 g/pot, M2: Acaulospora sp 20 g/pot dan M3: Glomus agregatum 10 g/pot ­+ Acaulosporasp 10 g/pot. Faktor kedua adalah jenis pupuk fosfat, yaitu P0: tanpa pupuk fosfat, P1: TSP 125 kg P2O5 /ha, P2: batuan fosfat (BP) 125 kg P2O5/ha dan P3: guano 125 kg P2O5/ha. Parameter penelitian adalah tinggi tanaman, jumlah daun, jumlah cabang, jumlah cabai dan berat kering tajuk. Data diolah dengan analisis ragam (uji F) dan dilanjutkan dengan uji DMRT. Hasil penelitian menunjukkan bahwa inokulasi G.agregatum + Acaulospora sp dapat meningkatkan jumlah daun. Inokulasi G.agregatum + Acaulospora sp, G.agregatum maupun Acaulospora sp dapat meningkatkan jumlah cabang dan jumlah cabai. Pemupukan TSP dapat meningkatkan jumlah cabang. Pemupukan TSP, BP maupun guano dapat meningkatkan tinggi tanaman, jumlah daun, jumlah cabai dan berat kering tajuk.Kata kunci : Capsicum frustescens, cendawan mikoriza arbuskular, TSP, batuan fosfat, guano 
Produksi biomassa ketumbar (Coriandrum sativum) dengan jarak tanam dan jenis pupuk hayati Maja Diego Armando Girsang; Budi Adi Kristanto; Dwi Retno Lukiwati
Journal of Agro Complex Vol 4, No 2 (2020): JOAC Online
Publisher : Department of Agriculture, Animal and Agricultural Sciences, Diponegoro University, Semara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/joac.4.2.108-115

Abstract

ABSTRACT The purpose of the research was to determine the effect of plant spacing and type of biofertilizer to the production of coriander biomass. This study used Randomized Block Design with two factors and repeated 3 times. The first factor was the different plant spacing (A) ie 20 × 20 cm (A0), 20 × 15 cm (A1) and 20 × 10 cm (A2). The second factor was the type of biofertilizer (B) ie without biofertilizer (B0), with biofertilizer A (B1) and with biofertilizer B (B2). The parameters observed were number of leaves, leaf area, leaf chlorophyll content, plant biomass per plant and production of plant biomass per plot. Data were analyzed using Duncan Multiple Range Test (DMRT) test of 5% level. The result of the analysis of different treatment range of spacing and the application of biofertilizer did not give interaction on the production of coriander biomass. Plant spacing of 20×20 cm (A0) increases leaf area and chlorophyll contents. Plant spacing of 20×10 cm (A2) yields the highest crop production per plot. The application of biofertilizer significantly increases the number of leaves and leaf area compared without biofertilizer. Keywords: Coriander, plant spacing and biofertilizer ABSTRAK  Penelitian ini bertujuan untuk mengetahui pengaruh jarak tanam dan jenis pupuk hayati berbeda terhadap produksi biomassa ketumbar. Penelitian ini menggunakan Rancangan Acak Kelompok dengan dua faktor dan diulang sebanyak 3 kali. Faktor pertama adalah jarak tanam yang berbeda (A) yaitu 20 × 20 cm (A0), 20 × 15 cm (A1) dan 20 × 10 cm (A2). Faktor kedua adalah jenis pupuk hayati (B) yaitu tanpa pupuk hayati (B0), dengan pupuk hayati A (B1) dan dengan pupuk hayati B (B2). Parameter yang diamati adalah jumlah daun, luas daun, kandungan klorofil daun, biomassa ketumbar per tanaman dan produksi biomassa ketumbar per petak. Data dianalisis dengan menggunakan uji Duncan Multiple Range Test (DMRT) taraf 5%. Hasil analisis ragam perlakuan jarak tanam dan jenis pupuk hayati berbeda tidak memberikan interaksi pada produksi biomassa ketumbar. Jarak tanam 20 × 10 cm (A0) meningkatkan luas daun dan kandungan klorofil. Jarak tanam 20 × 10 cm (A2) menghasilkan produksi tanaman per petak yang paling tinggi. Aplikasi pupuk hayati secara signifikan meningkatkan jumlah daun dan luas daun dibanding tanpa pupuk hayati. Kata kunci : Ketumbar, jarak tanam dan pupuk hayati. 
Pengaruh sistem tanam dan pupuk organik terhadap karakter agronomi turi dan rumput benggala pada tanah salin Rokhimun Tolib; Florentina Kusmiyati; Dwi Retno Lukiwati
Journal of Agro Complex Vol 1, No 2 (2017)
Publisher : Department of Agriculture, Animal and Agricultural Sciences, Diponegoro University, Semara

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (224.181 KB) | DOI: 10.14710/joac.1.2.57-64

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Penelitian bertujuan untuk mengkaji pengaruh sistem tanam serta dosis pupuk kandang terhadap karakter agronomi turi dan rumput benggala pada tanah salin. Salinitas tanah yang digunakan termasuk sedang, dengan daya hantar listrik 4,10 dS/m. Pupuk kandang (pukan) yang digunakan berasal dari ternak sapi. Rancangan percobaan yang digunakan, rancangan acak kelompok monofaktor terdiri dari 9 perlakuan dengan 3 kali ulangan, yaitu monokultur turi tanpa pukan (M1); monokultur turi + 10 ton pukan/ha (M2); monokultur turi + 20 ton pukan/ha (M3); tumpangsari turi dan rumput benggala + 0 ton pukan/ha (M4); tumpangsari turi dan rumput benggala + 10 ton pukan/ha (M5); tumpangsari turi dan rumput benggala + 20 ton pukan/ha (M6); monokultur rumput benggala tanpa pukan (M7); monokultur rumput benggala + 10 ton pukan/ha (M8); monokultur rumput benggala + 20 ton pukan/ha (M9). Hasil penelitian menunjukkan bahwa aplikasi pupuk kandang dengan dosis 10 ton/ha mampu meningkatkan jumlah daun, kadar klorofil a, kadar klorofil total, serta produksi bahan kering turi dan rumput benggala pada tanah salin. Sistem tanam tumpangsari mampu meningkatkan efisiensi penggunaan lahan. Kata kunci : Agronomi, sistem tanam, organik, turi, rumput benggala.
Respon pertumbuhan true shallot seed beberapa varietas bawang merah (Allium cepa l.) terhadap aplikasi giberelin Intan Shofa Elshyana; Dwi Retno Lukiwati; Karno Karno
Journal of Agro Complex Vol 3, No 3 (2019): Online
Publisher : Department of Agriculture, Animal and Agricultural Sciences, Diponegoro University, Semara

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (158.236 KB) | DOI: 10.14710/joac.3.3.114-123

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Penelitian ini bertujuan untuk mengetahui respon pertumbuhan beberapa varietas benih bawang merah terhadap beberapa dosis GA3 terhadap pertumbuhan bawang merah. Penelitian ini dilaksanakan pada tanggal 5 Maret – 6 Juli 2018 di Screen HouseAgroecotechnopark dan Laboratorium Fisiologi dan Pemuliaan Tanaman, Universitas Diponegoro, Semarang. Penelitian ini akan menggunakan Rancangan Acak Lengkap Faktorial 4x3 dengan 4 kali ulangan. Faktor pertama yaitu dosis GA3 terhadap terdiri atas 4 taraf yaitu M0: 0 ppm, M1: 50 pm, M2:100 ppm dan M3: 200 ppm. Faktor kedua yaitubeberapa varietas benih bawang merah terdiri atas 3 taraf yaitu V1: Bima, V2: Tutuk dan V3: Lokananta. Parameter yang diamati yaitu Indek vigor, daya kecambah, potensi tumbuh maksimum, tinggi tanaman, jumlah daun, jumlah anakan dan berat basah total tanaman. Data dianalisis ragam dan diuji lanjut dengan Duncan’s Multiple Range Test (DMRT). Hasil penelitian menunjukkan perlakuan dosis GA3 100 ppm dengan varietas Lokananta memberikan hasil nyata lebih tinggi dibanding perlakuan lainnya, pada parameter indeks vigor dan daya kecambah. Perlakuan dosis GA3 50 ppm dengan varietas tuktuk memberikan hasil nyata lebih tinggi dibanding perlakuan lainnya, pada jumlah daun dan anakan bawang merah. Kata Kunci : Allium cepa L.,true shallot seed, giberelin, pertumbuhan. 
Inokulasi berbagai spesies cendawan mikoriza arbuskular (CMA) terhadap pertumbuhan dan kemampuan akumulasi logam Pb pada tanaman marigold (Tagetes erecta L.) Fageta Dwi Safitri; Dwi Retno Lukiwati; I Mansur
Journal of Agro Complex Vol 4, No 1 (2020): JOAC Online
Publisher : Department of Agriculture, Animal and Agricultural Sciences, Diponegoro University, Semara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/joac.4.1.60-68

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ABSTRACTThis research aimed to indentify the impact of single species (G. fasciculatum, Acaulospora sp., G. etunicatum), double species, and multi-species AMF inoculation for plant growth and Pb accumulation ability of marigold plant. The research used monofactor randomized complete design with eight treatments and three replications.The treatments were M0. Control, M1. G. fasciculatum, M2. Acaulospora sp., M3. G. etunicatum, M4. G. fasciculatum + Acaulospora sp., M5. G. fasciculatum + G. etunicatum, M6. Acaulospora sp. + G. etunicatum, dan M7. G. fasciculatum + Acaulospora sp. + G. etunicatum. The parameters observed were plant height, number of branches, BCF, TF, and final concentration of Pb. Data analyzed using anova and followed by DMRT at α = 5%. The result indicated that AMF inoculation had significantly impact to the plant growth of marigold, BCF value, and Pb final concentration, but had no impact to the TF value. Inoculation of G. fasciculatum(M1) showed the highest plant growth of marigold. Inoculation of G. fasciculatum + Acaulospora sp. + G. Etunicatum(M7)showed the highest ability of Pb accumulation (BCF) and the lowest Pb final concentration in growing media. Based on BCF and TF values, the mechanism of Pb accumulation by marigold is phytoextraction.Keywords: Marigold, Pb, G. fasciculatum, Acaulospora sp., G. etunicatum. ABSTRAK Penelitian ini bertujuan untuk mengkaji pengaruh inokulasi CMA tunggal (G. fasciculatum, Acaulospora sp., G. etunicatum), ganda, dan multi-spesies terhadap pertumbuhan tanaman dan kemampuan akumulasi Pb pada marigold. Penelitian dilaksanakan menggunakan Rancangan Acak Lengkap (RAL) monofaktor dengan delapan perlakuan dan tiga ulangan. Perlakuan yang diberikan adalah M0. Kontrol, M1. G. fasciculatum, M2. Acaulospora sp., M3. G. etunicatum, M4. G. fasciculatum + Acaulospora sp., M5. G. fasciculatum + G. etunicatum, M6. Acaulospora sp. + G. etunicatum, dan M7. G. fasciculatum + Acaulospora sp. + G. etunicatum. Parameter penelitian meliputi tinggi tanaman, jumlah cabang, TF, BCF, dan konsentrasi Pb akhir media tanam. Data dianalisis ragam dan dilanjutkan uji jarak berganda Duncan pada taraf α = 5%. Hasil penelitian menunjukkan inokulasi berbagai spesies CMA berpengaruh terhadap pertumbuhan tanaman, nilai BCF, dan kadar Pb akhir media tanam, tetapi tidak berpengaruh terhadap nilai TF. Inokulasi G. fasciculatum(M1)memberikan pertumbuhan tanaman marigold tertinggi. InokulasiG. fasciculatum + Acaulospora sp, dan G. etunicatum(M7) menunjukkan kemampuan akumulasi Pb (BCF) tertinggi dan kadar Pb akhir media tanam terendah. Berdasarkan nilai BCF dan TF, mekanisme tanaman marigold dalam mengakumulasi logam Pb adalah fitoekstraksi.Kata kunci : Marigold, Pb, G. fasciculatum, Acaulospora sp., G. etunicatum. 
Pertumbuhan dan produksi jagung manis (Zea mays saccharata) dengan pemupukan “Kotpi Plus” Nyoman Adhitya Putra Dewangga; Dwi Retno Lukiwati; Budi Adi Kristanto
Journal of Agro Complex Vol 2, No 3 (2018)
Publisher : Department of Agriculture, Animal and Agricultural Sciences, Diponegoro University, Semara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/joac.2.3.229-234

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The research aimed to increase the quality of manure to “Kotpi Plus” and to study the effect on growth and yield of sweet corn. The research used monofactor randomized block design with six treatments and 5 replications. The treatments were T0. Control; T1. 100% “Kotpi Plus” + 0% Urea and TSP (UT); T2. 75% “Kotpi Plus” + 25% UT, T3. 50% “Kotpi Plus” + 50% UT; T4. 25% “Kotpi Plus” + 75% UT; and T5. 0% “Kotpi Plus” + 100% UT. Parameters measured were plant height, leaf number, cob length, cob weight with cornhusk, and sum of seed row in a cob. Data were subjected to analysis of variance and followed by DMRT at α = 5%. The result on plant height and leaf number showed that T4 167.7 cm was significantly higher compared to control and 8.21 leaves significantly higher compared to control and T1, respectively. “Kotpi Plus” combined with inorganic fertilizer produced cob length significantly higher compared to control. Cob weight with cornhusk on T4 was significantly higher compared to T0 and T1. The given of “Kotpi Plus” fertilizer was not affecting on sum of seed row in a cob. Keywords: Gliricidia sepium, growth, phosphate rock, sweet corn, yield.
Produksi dan nutrisi kedelai dengan inokulasi ganda (cendawan mikoriza arbuskular + Bradyrhizobium japonicum) dan pemupukan fosfat Artha Rizki Anggarani; Dwi Retno Lukiwati; D Wulandari
Journal of Agro Complex Vol 4, No 1 (2020): JOAC Online
Publisher : Department of Agriculture, Animal and Agricultural Sciences, Diponegoro University, Semara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/joac.4.1.16-22

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ABSTRACT The use of rock phosphate with mycorrhizal arbuscular fungi and rhizobium inoculation can be an alternative source to replace SP-36 role to increase soybean production. The objective of the research was to evaluate the effect of dual inoculation (Mycorrhizal Arbuscular Fungi+ Bradyrhizobium japonicum) and phosphate fertilization on dry weight, N and P straw concentration of soybean. The study used monofactor completely randomized design with five repliactions of treatments T0 (control), T1 (RP fertilizer), T2 (SP-36 fertilizer), T3 (mycorrhizalinoculation), T4 (B. japonicuminoculation), T5 (mycorrhizal+ RP), T6 (mycorrhizal + SP-36), T7 (B. japonicum + RP), T8 (B. japonicum + SP-36), T9 (dual inoculation), T10 (dual inoculation + RP), T11 (dual inoculation + SP-36). Parameters measured were seed weight, dry weight, N and P concentration of soybean straw. Data were analyzed by anova and DMRT at α = 5%. The results showed that T10 was non significant different on seed weight with T2. The T10 was higher in N and P straw concentration compared to T2 respectively. The use of RP with single mycorrhizal or double inculation could be recommended source of P to replace SP-36.Keywords: Dry weight, mycorrhiza, rhizobium, rock phosphate, soybean ABSTRAK Penggunaan batuan fosfat (BP) dengan inokulasi cendawan mikoriza arbuskular (CMA) dan rhizobium diharapkan mampu menggantikan peran SP-36 untuk meningkatkan produksi kedelai. Penelitian bertujuan untuk mengkaji pengaruh inokulasi ganda (Cendawan Mikoriza Arbuskular+ Bradyrhizobium japonicum) dan pemupukan fosfat terhadap produksi dan nutrisi kedelai. Penelitian menggunakan Rancangan Acak Lengkap (RAL) monofaktor lima ulangan dengan perlakuan T0 (kontrol), T1 (pupuk BP), T2 (pupuk SP-36), T3 (inokulasi CMA), T4 (inokulasi B. japonicum), T5 (CMA + BP), T6 (CMA + SP-36), T7 (B. japonicum + BP), T8 (B. japonicum + SP-36), T9 (inokulasi ganda), T10 (inokulasi ganda + BP), T11 (inokulasi ganda + SP-36). Parameter yang diamati adalah berat biji, berat kering jerami, kadar N dan P jerami kedelai. Data dianalisis ragam dan uji lanjut Duncan’s Multiple Range Test (DMRT) pada taraf α = 5%. Hasil biji dan berat kering jerami T10 tidak berbeda nyata terhadapT2. Perlakuan T10 menghasilkan kadar N dan P jerami nyata lebih tinggi dibanding perlakuan T2. Penggunaan BP dengan inokulasi CMA tunggal maupun ganda dapat menjadi alternatif sumber P yang lebih ramah lingkungan dibanding SP-36.Kata Kunci: Berat kering, batuan fosfat, kedelai, mikoriza, rhizobium 
PENINGKATAN PRODUKSI DAN KECERNAAN BAHAN KERING Centrosema pubescens DAN Pueraria phaseoloides OLEH PEMUPUKAN BATUAN FOSFAT DAN INOKULASI MVA Dwi Retno Lukiwati
Jurnal Ilmu-Ilmu Pertanian Indonesia Vol 9, No 1 (2007)
Publisher : Program Studi Ilmu Tanah Fakultas Pertanian UNIB

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/jipi.9.1.1-5

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Centrosema pubescens (centro) and Pueraria phaseoloides (puero) are important forage legumes as protein and mineral sources for ruminant livestock in the tropics.   However, most of the land that used for forage production is characterized by a low phosphorus content.   As the high cost of superphosphate is a major limiting factor, a combination of rock phosphate (RP) fertilization and vesicular-arbuscular mycorrhizae (VAM) fungi inoculation maybe a promising technique. A field experiment was conducted in Bogor during 7 months, on a latosolic soil (low pH and low available Bray II extractable P) to evaluate the effects of RP fertilization and VAM inoculation and their interaction on dry matter (DM) production and in vitro dry matter digestibility. A completely randomized block design with 3 replicates was used. The main experiment consisted therein the combination of three factors as follows 1) legume species (centro, puero), 2) VAM inoculation (with, and without VAM inoculation), and 3) rock phosphate fertilization (0, 44, 87, 131, and 175 kg P ha-1).  The period of defoliation was used as sub factor (defoliation I, II, and III).   Results showed that DM production and DM digestibility of puero was significantly higher (P<0.05) compared to centro after defoliation.  Dry matter digestibility of VAM inoculated puero was significantly higher (P<0.05) compared to uninoculated one. Rock phosphate fertilization significantly increased (P<0.05) DM production of VAM inoculated legume. Dry matter production was not significantly different (P>0.05) with or without VAM inoculation.  When inoculated, rock phosphate fertilization increased DM production of legume.   Success of VAM inoculation in the field affected by effectiveness of indigenous- VAM fungi or depending upon VAM inoculum potential.
PENINGKATAN PRODUKSI DAN NILAI NUTRISI HIJAUAN Pueraria phaseoloides OLEH PEMUPUKAN FOSFOR DALAM SUSPENSI FERMENTASI Acetobacter-Saccharomyces Dwi Retno Lukiwati; Novik Nurhidayat; C. Anggit Hatmaji Wibowo; J. Bambang Tri Nurdewanto
Jurnal Ilmu-Ilmu Pertanian Indonesia Vol 7, No 2 (2005)
Publisher : Program Studi Ilmu Tanah Fakultas Pertanian UNIB

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/jipi.7.2.82-86

Abstract

Puero (Pueraria phaseoloides) which is important legume as protein and mineral source is sensitive to the phosphorus deficiency. However, because of the high cost of superphosphate (SP), the focus is now on rock phosphate fertilizer (RP). Rock phosphate fertilizer, as one of the natural P sources, is relatively cheaper than SP but it slowly available to plants. Fermentation solution of Acetobacter-Saccharomyces (FSAS) maybe a promising technique to overcome this problem. A greenhouse experiment was conducted during 8 weeks on acid latosolic soil and low phosphorus availability. A completely randomized design with 5 treatments, and 4 replicates was used. The treatments were contro, RP, SP, RP+FSAS, and SP+FSAS. Phosphorus fertilizer level was 200 kg P2O5 ha-1 (2.22 g RP per pot or 1.67 g SP per pot). Puero was cut and measured for dry matter (DM), crude protein content (CP), S and Se content on 8 weeks after planting. All data were analyzed by the GLM procedure of SAS. Significant differences among the treatments were calculated by DMRT. The results show that SP+FSAS increased DM production, CP and Se content significantly higher compared to SP (P<0.05). However, RP+FSAS resulted DM production, CP and S content of puero did not difference compared to SP, but Se content significantly higher (P<0.05). Phosphorus fertilization (RP, SP) + FSAS could increase Se content of puero. No differences in DM production, CP and S content were found between RP+FSAS compared to SP fertilization.